ViewGene
Locus Name:
GeneID:
Location:

GeneID:5876
TrivialName: PA14_37970
AnnotatorUID: danlee
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_37970
PAO1 Ortholog Locus:
Sequence Length:321
Protein Length:106
Start: 3387894
Stop: 3388213
Strand: +
Type:
ChromosomeID:2
Status: INACTIVE
Frame Discrepancy: TRUE
Missense Discrepancy:TRUE
Comments:
Inactivated 2/01/06. PA14_37960 (GeneID 5877) and PA14_37970 (GeneID 5876) replaced by PA14_37965 (GeneID 7379). Homology: gb|AAG05440.1|AE004631_9 (AE004631) cyanate lyase [Pseudomonas aeruginosa PAO1]
Identities = 82/82 (100%), Positives = 82/82 (100%)
Sequence:
atgccctgccagaggcggcggcgcgcaaggtcgccgcccaactcggcatggacgacgacgCGGtgCtcctgc
ttgcaaAGCATCCCGCTGcGcggCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTAC
GAGATGCTGCAAGTCTACGGCAGCACCCTGAAGGCACTGGTCCACGAACAGTTCGGCGACGGCATCATCAGC
GCGATCAACTTCAAGCTGGACATCAAGAAGGTCGAGGACCCGGAAGGCGGCTCGCGGGCGGTGATCACCCTC
GACGGCAAGTACCTGCCGACCAAACCGTTCTGA
Translation:
MPCQRRRRARSPPNSAWTTTRCSCLQSIPLRGSIPGGIPSDPTIYRFYEMLQVYGSTLKALVHEQFGDGIIS
AINFKLDIKKVEDPEGGSRAVITLDGKYLPTKPF*
AnnotationID:5877GeneID:5876
AnnotatorUID: danlee
Modification Date/Time: 2006-02-01 16:04:31
Gene Name:
Confidence Code:4
GeneProduct:
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:()
Alternate Gene Product Name:cyanase
Functional Category Confidence Code:5
COGs:pfam02560
Secondary Functional Category(ies):central intermediary metabolism
EC Number:4.3.99.1
Status:INACTIVE
Pathway:






 
Homology:
>gb|AAG54689.1|AE005213_5 (AE005213) cyanate aminohydrolase, cyanase [Escherichia coli
           O157:H7 EDL933]
          Length = 156

 Score =  145 bits (367), Expect = 3e-35
 Identities = 70/82 (85%), Positives = 75/82 (91%)

>gb|AAO55448.1| (AE016862) cyanate lyase [Pseudomonas syringae pv. tomato str.
           DC3000]
          Length = 155

 Score =  137 bits (344), Expect = 2e-32
 Identities = 67/82 (81%), Positives = 75/82 (91%), Gaps = 1/82 (1%)








 
Structural Features:
pfam02560, Cyanate_lyase, Cyanate lyase C-terminal domain.
CD-Length = 74 residues,  93.2% aligned
Score =  119 bits (301), Expect = 4e-29
Identities = 47/69 (68%), Positives = 58/69 (84%)







   
Genomic Context:






   
Comment:
Inactivated 2/01/06. PA14_37960 (GeneID 5877) and PA14_37970 (GeneID 5876) replaced by PA14_37965 (GeneID 7379).


















    ReferenceID:11665




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:28872




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32649




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:5876_cdd.html


    		




 
URL:

















    ReferenceID:34393




  
Author/Investigator(s):
        Anderson PM, Sung YC, Fuchs JA.





  
Title:
        The cyanase operon and cyanate metabolism.




  
PubMed:
        2094285


MedLine:
        




    
Source:
FEMS Microbiol Rev. 1990 Dec;7(3-4):247-52.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2094285

















    ReferenceID:34396




  
Author/Investigator(s):
        Sung YC, Fuchs JA.





  
Title:
        Identification and characterization of a cyanate permease in Escherichia coli K-12.




  
PubMed:
        2670891


MedLine:
        




    
Source:
J Bacteriol. 1989 Sep;171(9):4674-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2670891

















    ReferenceID:34397




  
Author/Investigator(s):
        Sung YC, Fuchs JA.





  
Title:
        Characterization of the cyn operon in Escherichia coli K12.




  
PubMed:
        3049588


MedLine:
        




    
Source:
J Biol Chem. 1988 Oct 15;263(29):14769-75.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3049588

















    ReferenceID:34400




  
Author/Investigator(s):
        Sung YC, Anderson PM, Fuchs JA.





  
Title:
        Characterization of high-level expression and sequencing of the Escherichia coli K-12 cynS gene encoding cyanase.




  
PubMed:
        2822670


MedLine:
        




    
Source:
J Bacteriol. 1987 Nov;169(11):5224-30.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2822670









           
Homologs By Global Alignment 

           

       



     
Gene ID:5876
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
7928
                           PA2052_tr
                           translation of PA2052
                           158
                           53.16
                           55.06
                           54
                           410.0
     

     
     
197894
                           gb|AAG05440.1|AE004631_9
                           (AE004631) cyanate lyase [Pseudomonas aeruginosa PAO1]
                           158
                           53.16
                           55.06
                           54
                           410.0
     

     
     
197896
                           emb|CAE12407.1|
                           (BX571859) cyanate lyase (cyanate hydrolase) (cyanase) [Photorhabdus luminescens subsp. laumondii TTO1]
                           162
                           48.14
                           54.32
                           62
                           387.0
     

     
     
197903
                           gb|AAO55448.1|
                           (AE016862) cyanate lyase [Pseudomonas syringae pv. tomato str. DC3000]
                           156
                           46.79
                           55.12
                           51
                           332.0
     

     
     
197897
                           gb|AAG54689.1|AE005213_5
                           (AE005213) cyanate aminohydrolase, cyanase [Escherichia coli O157:H7 EDL933]
                           158
                           45.56
                           51.26
                           54
                           355.0
     

     






      

          
GC ORFID: 48878How Found: Glimmer

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: Glimmer Score: 

          
Start: 1566017Stop: 1565697

          
Length: 321


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2052 translation of PA2052Homolog Bit Score 167.0

          
Other Homologs:  t_PA2052 translation of PA2052 (BLASTP 167), psyr_15may02_Scaffold8_revised_gene7887 related to: gi|15597248|ref|NP_250742.1| (NC_002516) cyanate lyase [Pseudomonas aeruginosa] pir||C83390 cyanate lyase PA2052 [imported] - Pseudomonas aeruginosa (strain PAO1) gb|AAG05440.1|AE004631_9 (AE004631) cyanate lyase [Pseudomonas aeruginosa] (BLASTP 137)

          
GC ORF Sequence

           
atgccctgccagaggcggcggcgcgcaaggtcgccgcccaactcggcatggacgacgacgCGGtgCtcctgc 
ttgcaaAGCATCCCGCTGcGcggCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTAC 
GAGATGCTGCAAGTCTACGGCAGCACCCTGAAGGCACTGGTCCACGAACAGTTCGGCGACGGCATCATCAGC 
GCGATCAACTTCAAGCTGGACATCAAGAAGGTCGAGGACCCGGAAGGCGGCTCGCGGGCGGTGATCACCCTC 
GACGGCAAGTACCTGCCGACCAAACCGTTCTGA