ViewGene
Locus Name:
GeneID:
Location:

GeneID:5866
TrivialName: PA14_38390
AnnotatorUID: danlee
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_38390
PAO1 Ortholog Locus:
Sequence Length:657
Protein Length:218
Start: 3421638
Stop: 3422295
Strand: +
Type:
ChromosomeID:2
Status: INACTIVE
Frame Discrepancy: TRUE
Missense Discrepancy:TRUE
Comments:
Inactivated 9-26-05. Replaced by 7358 (which combined 5866 and 3927). Homology: gb|AAG05407.1|AE004628_5 (AE004628) RND multidrug efflux membrane fusion protein precursor [Pseudomonas aeruginosa PAO1]
Identities = 190/191 (99%), Positives = 190/191 (99%)
Sequence:
GTGCCCCTTCCCGGGCAAGCTCGCGAGTTCACGAATCGAGGGACACCCATGCACATCCAATGGACCGGCTCG
CTGCGCGGGCTGCTGGCGGCCCTGGTCGCCCTATTCCTGCTGGGCTGCGAAGAAGCAGCGGACACCGGGAAG
ACTGCGGAGGCCCCCGCCGAGGTCGGCGTGATCGTCGCCAGGCCGGCGCCCATCGGCATCACCAGCGAGCTG
CCCGGACGCCTGGAAGCGTACCGCCAGGCCGAGGTGCGGGCGCGCGTCGCCGGCATCGTCACCCGTCGCCTG
TACGaggAaggccagGACGTCCGCGCCGGCACCGTGCTGTTCcaGATCGACCCGGCGCCCTTgaaggcgGCC
CTGGACATCAGCCGCGGCGCCCTGGCCCGGGCcgaggcCAGCCACGCGGCGGCGGCCGACAAGCTCAAGCGC
TACGCCGACCTGATCAagGACCGCGCCATCAGCGAACGCGAGTACACCGAAGCGCAGACCGACGCGCGCCAG
GCCCTGGCGCAGATCGCCTCGgCcaagGCCGAACtggagcAgGCCCGCCTGCGCCTGGGCTACGCCACGGTC
ACCGCGCCGATCGACGGCCGCGCGCGGCGCGCGCTGGTcacCGaagcgcgctggtcGGcgaggacTcGCCGA
CACCGCTGA
Translation:
MPLPGQAREFTNRGTPMHIQWTGSLRGLLAALVALFLLGCEEAADTGKTAEAPAEVGVIVARPAPIGITSEL
PGRLEAYRQAEVRARVAGIVTRRLYEEGQDVRAGTVLFQIDPAPLKAALDISRGALARAEASHAAAADKLKR
YADLIKDRAISEREYTEAQTDARQALAQIASAKAELEQARLRLGYATVTAPIDGRARRALVTEARWSARTRR
HR*
AnnotationID:5867GeneID:5866
AnnotatorUID: danlee
Modification Date/Time: 2005-09-26 14:02:56
Gene Name:amrA
Confidence Code:
GeneProduct:putative membrane fusion protein
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:()
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:
Secondary Functional Category(ies):transport of small molecules
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAC27753.1| (AF072887) putative membrane fusion protein AmrA [Burkholderia
           pseudomallei]
          Length = 399

 Score =  207 bits (527), Expect = 4e-53
 Identities = 109/191 (57%), Positives = 139/191 (72%)

>gb|AAN68425.1|AE016475_4 (AE016784) multidrug efflux RND membrane fusion protein MexC
           [Pseudomonas putida KT2440]
          Length = 378

 Score =  169 bits (428), Expect = 1e-41
 Identities = 95/187 (50%), Positives = 123/187 (65%), Gaps = 3/187 (1%)

>gb|AAN69057.1|AE016538_6 (AE016786) multidrug efflux RND membrane fusion protein
           [Pseudomonas putida KT2440]
          Length = 391

 Score =  166 bits (419), Expect = 1e-40
 Identities = 93/179 (51%), Positives = 123/179 (68%), Gaps = 1/179 (0%)








 
Structural Features:






   
Genomic Context:






   
Comment:
possible frameshift; see gene #3927


















    ReferenceID:15729




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:28855




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32676




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:5866_cdd.html


    		




 
URL:

















    ReferenceID:34387




  
Author/Investigator(s):
        Wang W, Zhang W, Chen H, Chiao J, Zhao G, Jiang W.





  
Title:
        Molecular and biochemical characterization of a novel two-component signal transduction system, amrA- amkA, involved in rifamycin SV production in Amycolatopsis mediterranei U32.




  
PubMed:
        12375106


MedLine:
        




    
Source:
Arch Microbiol. 2002 Nov;178(5):376-86. Epub 2002 Aug 22.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12375106

















    ReferenceID:34390




  
Author/Investigator(s):
        Ribardo DA, McIver KS.





  
Title:
        amrA encodes a putative membrane protein necessary for maximal exponential phase expression of the Mga virulence regulon in Streptococcus pyogenes.




  
PubMed:
        14617188


MedLine:
        




    
Source:
Mol Microbiol. 2003 Oct;50(2):673-85.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=14617188









           
Homologs By Global Alignment 

           

       



     
Gene ID:5866
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
7926
                           PA2019_tr
                           translation of PA2019
                           412
                           46.60
                           47.08
                           210
                           915.0
     

     
     
197816
                           gb|AAG05407.1|AE004628_5
                           (AE004628) RND multidrug efflux membrane fusion protein precursor [Pseudomonas aeruginosa PAO1]
                           412
                           46.60
                           47.08
                           210
                           915.0
     

     
     
197817
                           gb|AAF14522.1|AF147719_2
                           (AF147719) putative membrane fusion protein; AmrA [Pseudomonas aeruginosa]
                           412
                           46.60
                           47.08
                           210
                           915.0
     

     
     
197822
                           gb|AAD22454.1|AF116283_2
                           (AF116283) membrane fusion protein [Pseudomonas aeruginosa]
                           218
                           43.57
                           43.57
                           121
                           470.0
     

     
     
197818
                           dbj|BAA34299.1|
                           (AB015853) MexX [Pseudomonas aeruginosa]
                           410
                           41.95
                           42.43
                           213
                           784.0
     

     






      

          
GC ORFID: 48868How Found: Glimmer

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: Glimmer Score: 

          
Start: 1532272Stop: 1531616

          
Length: 657


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2019 translation of PA2019Homolog Bit Score 360.0

          
Other Homologs:  t_PA2019 translation of PA2019 (BLASTP 360), t_PA4599 translation of PA4599 (BLASTP 169), psyr_15may02_Scaffold1_revised_gene220 related to: gi|19070022|emb|CAD24338.1| (AJ431260) MexC protein [uncultured bacterium] (BLASTP 155)

          
GC ORF Sequence

           
GTGCCCCTTCCCGGGCAAGCTCGCGAGTTCACGAATCGAGGGACACCCATGCACATCCAATGGACCGGCTCG 
CTGCGCGGGCTGCTGGCGGCCCTGGTCGCCCTATTCCTGCTGGGCTGCGAAGAAGCAGCGGACACCGGGAAG 
ACTGCGGAGGCCCCCGCCGAGGTCGGCGTGATCGTCGCCAGGCCGGCGCCCATCGGCATCACCAGCGAGCTG 
CCCGGACGCCTGGAAGCGTACCGCCAGGCCGAGGTGCGGGCGCGCGTCGCCGGCATCGTCACCCGTCGCCTG 
TACGaggAaggccagGACGTCCGCGCCGGCACCGTGCTGTTCcaGATCGACCCGGCGCCCTTgaaggcgGCC 
CTGGACATCAGCCGCGGCGCCCTGGCCCGGGCcgaggcCAGCCACGCGGCGGCGGCCGACAAGCTCAAGCGC 
TACGCCGACCTGATCAagGACCGCGCCATCAGCGAACGCGAGTACACCGAAGCGCAGACCGACGCGCGCCAG 
GCCCTGGCGCAGATCGCCTCGgCcaagGCCGAACtggagcAgGCCCGCCTGCGCCTGGGCTACGCCACGGTC 
ACCGCGCCGATCGACGGCCGCGCGCGGCGCGCGCTGGTcacCGaagcgcgctggtcGGcgaggacTcGCCGA 
CACCGCTGA