ViewGene
Locus Name:
GeneID:
Location:

GeneID:575
TrivialName: PA14_29490
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_29490
PAO1 Ortholog Locus:PA2677
Sequence Length:1728
Protein Length:575
Start: 2557190
Stop: 2558917
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06065.1|AE004695_18 (AE004695) probable type II secretion protein [Pseudomonas aeruginosa PAO1]
Identities = 574/575 (99%), Positives = 575/575 (100%)
Sequence:
ATGTCCTCTGAGGCCGTTTCCCCGGGCCCGCAGGTGACGGCCGAGTGGATACCGCTGGGGCAGCGTTTGCTC
GAACGCGGGTTGGTGAGCGGCCAGGAACTCGAGCGGGCGCTCGACCTGCAACGCCGGCTTGGCGGCCGCCTG
GGAGGTATTCTCGTGCGTTCCGGAGCCATCTCGGAAAATACCCTCATGCAGGTGCTGGCGGAGCAACTGCGG
TTGCCGCTGGTGGGGGACGACCTGCGGAAGCCGAGCGAAGAGTCCATCGCCGCATTCCTGCTCAGCACCCCG
ATCAATACCGACTGGTTCGTCGAAGAGCAACTGGTGGTCTGGGAGGAGGGCGAGCAACTGCTGTTCGCGGCG
CGTGATCCGTTATCTCCTTCGATACGCGAAACCCTGGGCTATTTCTATCCGGAGCGCTCGATTCAAGCAGTG
CTGTGCCGCTCCCAGGATCTGGACGGCTGGTTGGAGCATTCCCTGGACCTGGCCCGGCAAGGTGCGGATCGG
CACGCCTACGGCAGCGAAGACATCCGCTACCTGCGGGAGCTGGCGGAGGAGGCGCCGGTAGTCGAACTCGTC
AACAACGTGATGAGCCAGGCCATGGAAAAGCGCTCTTCGGATATTCACATCGAACCCCGGGAGTTCGACTTC
CGTATCCGCTTCCGCGTCGACGGCGTCCTGCACGACCAACTGAGCCTTCCCCGCGAGCGATTCGCGGCGGTT
GTCTCGCGGATCAAGCTGATCTCCGCCATCGACATCGCGGAGCGACGGTTGCCGCAGGATGGACGCATGAGC
ATCCGGGTCAGCGGTACGGAGCTCGACGTGCGGGTCTCCACCTTGCCAGGGGTGCATGGCGAATCGGTGGTC
ATGCGCCTGTTGCCGAAGGAGCGCGAGGGGTTGCGCCTGGACCGGCTCGGGATGCTCCCGGACCACTTGCGG
AGGATGCGTGCATGGGTAGCGGAACCCCACGGCATCATCCTGGTGACCGGCCCCACCGGATCGGGCAAGTCC
ACCACGCTCTATGGCGCGCTGGAGGAAATCAATGATGGCGTGCGCAAGCTCATCAGCGTGGAAGATCCCGTG
GAGTACCAGGTGCCCAACATCACCCAGGTCCAGGTCCATGCCGACATCGGCCTGACCTTCGCTGCCGCCTTG
CGCTCGATCCTGCGGCAGGATCCCGATGTGATCATGATCGGGGAGATACGCGACCTGGAGACGGCGGAGATC
GCCGTACAGTCCTCGCTCACCGGTCACCTGGTGCTTTCGACCCTGCATACCAACGATGCGGTGAGTGCCTTC
ACGCGCTTGATCGACATGGGCGTGGAACCCTTCCTGGTGGCTTCGCCGGTGCGCGGTGTCCAGGCCCAGCGA
CTGGTGCGCAGGTTGTGCGCCCACTGCGCGGAACCTTGCGAGCCAACCCTTGCTGCGGCCGATCTGGCAGCG
ACCGAGGCGGCCACCGCGCGGCTGTTCCCGGGGCAGGCAGCGCAATGGCGCGTGGCGCGAGGCTGCGGCCGC
TGCGAAGGTACCGGCTACAGCGGCCGTGTGGGCATCTACGAACTCGTCGATCTGTCTTCCGAGATGCGCGAC
CTGATCGTGCAGAGAGCTTCCCTCGAACAGATGCATCGGCTTGCCGCCAGCCAGGGACAGCGCAATCTGCGT
GAAGATGGCTTGATCAAGGCCTGGTTGGGTATGACCAGTCTTGACGAGGTGCATCGCGTGACCAGCGGCTAA
Translation:
MSSEAVSPGPQVTAEWIPLGQRLLERGLVSGQELERALDLQRRLGGRLGGILVRSGAISENTLMQVLAEQLR
LPLVGDDLRKPSEESIAAFLLSTPINTDWFVEEQLVVWEEGEQLLFAARDPLSPSIRETLGYFYPERSIQAV
LCRSQDLDGWLEHSLDLARQGADRHAYGSEDIRYLRELAEEAPVVELVNNVMSQAMEKRSSDIHIEPREFDF
RIRFRVDGVLHDQLSLPRERFAAVVSRIKLISAIDIAERRLPQDGRMSIRVSGTELDVRVSTLPGVHGESVV
MRLLPKEREGLRLDRLGMLPDHLRRMRAWVAEPHGIILVTGPTGSGKSTTLYGALEEINDGVRKLISVEDPV
EYQVPNITQVQVHADIGLTFAAALRSILRQDPDVIMIGEIRDLETAEIAVQSSLTGHLVLSTLHTNDAVSAF
TRLIDMGVEPFLVASPVRGVQAQRLVRRLCAHCAEPCEPTLAAADLAATEAATARLFPGQAAQWRVARGCGR
CEGTGYSGRVGIYELVDLSSEMRDLIVQRASLEQMHRLAASQGQRNLREDGLIKAWLGMTSLDEVHRVTSG*
AnnotationID:584GeneID:575
AnnotatorUID: diggins
Modification Date/Time: 2005-03-07 18:06:59
Gene Name:
Confidence Code:3
GeneProduct:putative type II secretion protein
Cell Localization:()
Synonyms:hplR
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(19) Protein secretion/export apparatus
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG2804,cd01129,pfam00437
Secondary Functional Category(ies):intracellular trafficking and secretion
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN49573.1|AE011406_12 (AE011406) general secretion pathway protein E [Leptospira
           interrogans serovar lai str. 56601]
          Length = 557

 Score =  414 bits (1063), Expect = e-114
 Identities = 234/570 (41%), Positives = 352/570 (61%), Gaps = 31/570 (5%)

>gb|AAO56795.1| (AE016867) general secretion pathway protein E [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 585

 Score =  403 bits (1036), Expect = e-111
 Identities = 253/568 (44%), Positives = 342/568 (60%), Gaps = 34/568 (5%)








 
Structural Features:
COG2804, PulE, Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, 
ATPase PilB 
[Cell motility and secretion / Intracellular trafficking and secretion]
CD-Length = 500 residues,  98.2% aligned
Score =  529 bits (1364), Expect = 3e-151
Identities = 241/502 (48%), Positives = 325/502 (64%), Gaps = 24/502 (4%)

cd01129, PulE-GspE
CD-Length = 264 residues, 100.0% aligned
Score =  358 bits (920), Expect = 1e-99

pfam00437, GSPII_E, Type II/IV secretion system protein.
CD-Length = 283 residues, 100.0% aligned
Score =  242 bits (620), Expect = 7e-65
Identities = 123/291 (42%), Positives = 171/291 (58%), Gaps = 20/291 (6%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1153




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18295




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36348




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:575_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:575
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
983
                           PA2677_tr
                           translation of PA2677
                           575
                           99.82
                           99.99
                           0
                           2899.0
     

     
     
38925
                           gb|AAG06065.1|AE004695_18
                           (AE004695) probable type II secretion protein [Pseudomonas aeruginosa PAO1]
                           575
                           99.82
                           99.99
                           0
                           2899.0
     

     
     
38929
                           gb|AAM39976.1|
                           (AE012165) general secretion pathway protein E [Xanthomonas campestris pv. campestris str. ATCC 33913]
                           617
                           42.94
                           57.37
                           80
                           1066.0
     

     
     
38931
                           gb|AAC27375.3|
                           (L02630) XpsE [Xanthomonas campestris]
                           622
                           41.96
                           55.46
                           102
                           1053.0
     

     
     
38928
                           gb|AAO56795.1|
                           (AE016867) general secretion pathway protein E [Pseudomonas syringae pv. tomato str. DC3000]
                           630
                           41.58
                           55.55
                           100
                           1077.0
     

     






      

          
GC ORFID: 43577How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2677Glimmer Score: 

          
Start: 2396736Stop: 2395009

          
Length: 1728


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA2677 translation of PA2677Homolog Bit Score 1121.0

          
Other Homologs:  psyr_15may02_Scaffold11_revised_gene1763 (ORF: BLASTX 405.0), t_PA0686 (ORF: BLASTX 392.0), t_PA3103 (ORF: BLASTX 391.0), psyr_15may02_Scaffold2_revised_gene3914 (ORF: BLASTX 332.0), t_PA2677 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCCTCTGAGGCCGTTTCCCCGGGCCCGCAGGTGACGGCCGAGTGGATACCGCTGGGGCAGCGTTTGCTC 
GAACGCGGGTTGGTGAGCGGCCAGGAACTCGAGCGGGCGCTCGACCTGCAACGCCGGCTTGGCGGCCGCCTG 
GGAGGTATTCTCGTGCGTTCCGGAGCCATCTCGGAAAATACCCTCATGCAGGTGCTGGCGGAGCAACTGCGG 
TTGCCGCTGGTGGGGGACGACCTGCGGAAGCCGAGCGAAGAGTCCATCGCCGCATTCCTGCTCAGCACCCCG 
ATCAATACCGACTGGTTCGTCGAAGAGCAACTGGTGGTCTGGGAGGAGGGCGAGCAACTGCTGTTCGCGGCG 
CGTGATCCGTTATCTCCTTCGATACGCGAAACCCTGGGCTATTTCTATCCGGAGCGCTCGATTCAAGCAGTG 
CTGTGCCGCTCCCAGGATCTGGACGGCTGGTTGGAGCATTCCCTGGACCTGGCCCGGCAAGGTGCGGATCGG 
CACGCCTACGGCAGCGAAGACATCCGCTACCTGCGGGAGCTGGCGGAGGAGGCGCCGGTAGTCGAACTCGTC 
AACAACGTGATGAGCCAGGCCATGGAAAAGCGCTCTTCGGATATTCACATCGAACCCCGGGAGTTCGACTTC 
CGTATCCGCTTCCGCGTCGACGGCGTCCTGCACGACCAACTGAGCCTTCCCCGCGAGCGATTCGCGGCGGTT 
GTCTCGCGGATCAAGCTGATCTCCGCCATCGACATCGCGGAGCGACGGTTGCCGCAGGATGGACGCATGAGC 
ATCCGGGTCAGCGGTACGGAGCTCGACGTGCGGGTCTCCACCTTGCCAGGGGTGCATGGCGAATCGGTGGTC 
ATGCGCCTGTTGCCGAAGGAGCGCGAGGGGTTGCGCCTGGACCGGCTCGGGATGCTCCCGGACCACTTGCGG 
AGGATGCGTGCATGGGTAGCGGAACCCCACGGCATCATCCTGGTGACCGGCCCCACCGGATCGGGCAAGTCC 
ACCACGCTCTATGGCGCGCTGGAGGAAATCAATGATGGCGTGCGCAAGCTCATCAGCGTGGAAGATCCCGTG 
GAGTACCAGGTGCCCAACATCACCCAGGTCCAGGTCCATGCCGACATCGGCCTGACCTTCGCTGCCGCCTTG 
CGCTCGATCCTGCGGCAGGATCCCGATGTGATCATGATCGGGGAGATACGCGACCTGGAGACGGCGGAGATC 
GCCGTACAGTCCTCGCTCACCGGTCACCTGGTGCTTTCGACCCTGCATACCAACGATGCGGTGAGTGCCTTC 
ACGCGCTTGATCGACATGGGCGTGGAACCCTTCCTGGTGGCTTCGCCGGTGCGCGGTGTCCAGGCCCAGCGA 
CTGGTGCGCAGGTTGTGCGCCCACTGCGCGGAACCTTGCGAGCCAACCCTTGCTGCGGCCGATCTGGCAGCG 
ACCGAGGCGGCCACCGCGCGGCTGTTCCCGGGGCAGGCAGCGCAATGGCGCGTGGCGCGAGGCTGCGGCCGC 
TGCGAAGGTACCGGCTACAGCGGCCGTGTGGGCATCTACGAACTCGTCGATCTGTCTTCCGAGATGCGCGAC 
CTGATCGTGCAGAGAGCTTCCCTCGAACAGATGCATCGGCTTGCCGCCAGCCAGGGACAGCGCAATCTGCGT 
GAAGATGGCTTGATCAAGGCCTGGTTGGGTATGACCAGTCTTGACGAGGTGCATCGCGTGACCAGCGGCTAA