ViewGene
Locus Name:
GeneID:
Location:

GeneID:4846
TrivialName: PA14_02630
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_02630
PAO1 Ortholog Locus:PA0215
Sequence Length:405
Protein Length:134
Start: 233582
Stop: 233986
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03604.1|AE004459_8 (AE004459) probable transporter [Pseudomonas aeruginosa PAO1]
Identities = 132/134 (98%), Positives = 132/134 (98%)
Sequence:
ATGATTATCTACGGTGTCGCACTGCTGTCTCTGTGTACCCTCGCCGGGCTGTTCATCGGCGAGATCCTTGGC
GTGCTGCTCGGCGTGCCGGCCAACGTCGGTGGCGTCGGCATCGCCATGCTGCTGCTGATCTTCGTCGGCGGC
TACCTGGGCAAGCGCGGCCTGCTCTCGGCGAAGACCGAGCAGGGCGTGGAGTTCTGGAGCGCGGTCTACATT
CCCATCGTGGTCGCCATGGCCGCCCAGCAGAACGTCCTCGGCGCGCTGAGCGGCGGGCCGGCCGCTATCCTC
GCCGGCATCGCCGCGGTCGCCGTGGCCTTCGCCCTGGTCCCGCTGCTCGACCGCTTCGGCCGGAAGAAAGCC
GAAGGCGCCCCGCCGTTGAAACCGCGCACCAGCGTGCAGGGGTGA
Translation:
MIIYGVALLSLCTLAGLFIGEILGVLLGVPANVGGVGIAMLLLIFVGGYLGKRGLLSAKTEQGVEFWSAVYI
PIVVAMAAQQNVLGALSGGPAAILAGIAAVAVAFALVPLLDRFGRKKAEGAPPLKPRTSVQG*
AnnotationID:4851GeneID:4846
AnnotatorUID: diggins
Modification Date/Time: 2005-03-23 12:12:37
Gene Name:
Confidence Code:3
GeneProduct:putative malonate carrier protein
Cell Localization:()
Synonyms:mdcL
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:putattve malonate transporter, MadL subunit
Functional Category Confidence Code:5
COGs:pfam03817
Secondary Functional Category(ies):membrane proteins
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>dbj|BAA36211.1| (AB017138) malonate transporter [Pseudomonas putida]
          Length = 163

 Score =  140 bits (353), Expect = 1e-33
 Identities = 75/133 (56%), Positives = 94/133 (70%), Gaps = 2/133 (1%)

>gb|AAF20286.1|AF121266_3 (AF121266) malonate carrier protein MdcL [Acinetobacter
           calcoaceticus]
          Length = 133

 Score =  113 bits (283), Expect = 2e-25
 Identities = 58/114 (50%), Positives = 80/114 (70%)








 
Structural Features:
pfam03817, MadL, Malonate transporter MadL subunit.
CD-Length = 125 residues, 100.0% aligned
Score =  148 bits (376), Expect = 2e-37
Identities = 86/125 (68%), Positives = 100/125 (80%)






   
Genomic Context:






   
Comment:
"madL" also a possible gene name


















    ReferenceID:9692




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:26833




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33579




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:4846_cdd.html


    		




 
URL:

















    ReferenceID:35461




  
Author/Investigator(s):
        Chohnan S, Kurusu Y, Nishihara H, Takamura Y.





  
Title:
        Cloning and characterization of mdc genes encoding malonate decarboxylase from Pseudomonas putida.




  
PubMed:
        10339824


MedLine:
        




    
Source:
FEMS Microbiol Lett. 1999 May 15;174(2):311-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10339824









           
Homologs By Global Alignment 

           

       



     
Gene ID:4846
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
7223
                           PA0215_tr
                           translation of PA0215
                           134
                           98.50
                           98.50
                           0
                           642.0
     

     
     
188853
                           gb|AAG03604.1|AE004459_8
                           (AE004459) probable transporter [Pseudomonas aeruginosa PAO1]
                           134
                           98.50
                           98.50
                           0
                           642.0
     

     
     
188855
                           gb|AAO58507.1|
                           (AE016874) malonate transporter, MadL subunit [Pseudomonas syringae pv. tomato str. DC3000]
                           145
                           49.65
                           65.51
                           15
                           342.0
     

     
     
188857
                           gb|AAC45407.1|
                           (U87980) MadL [Malonomonas rubra]
                           134
                           48.50
                           67.16
                           5
                           313.0
     

     
     
188856
                           dbj|BAC46542.1|
                           (AP005939) malonate carrier protein [Bradyrhizobium japonicum USDA 110]
                           145
                           48.27
                           63.44
                           12
                           337.0
     

     






      

          
GC ORFID: 47848How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0215Glimmer Score: 

          
Start: 6034923Stop: 6035327

          
Length: 405


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0215 translation of PA0215Homolog Bit Score 251.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3324 (ORF: BLASTX 137.0), t_PA0215 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGATTATCTACGGTGTCGCACTGCTGTCTCTGTGTACCCTCGCCGGGCTGTTCATCGGCGAGATCCTTGGC 
GTGCTGCTCGGCGTGCCGGCCAACGTCGGTGGCGTCGGCATCGCCATGCTGCTGCTGATCTTCGTCGGCGGC 
TACCTGGGCAAGCGCGGCCTGCTCTCGGCGAAGACCGAGCAGGGCGTGGAGTTCTGGAGCGCGGTCTACATT 
CCCATCGTGGTCGCCATGGCCGCCCAGCAGAACGTCCTCGGCGCGCTGAGCGGCGGGCCGGCCGCTATCCTC 
GCCGGCATCGCCGCGGTCGCCGTGGCCTTCGCCCTGGTCCCGCTGCTCGACCGCTTCGGCCGGAAGAAAGCC 
GAAGGCGCCCCGCCGTTGAAACCGCGCACCAGCGTGCAGGGGTGA