ViewGene
Locus Name:
GeneID:
Location:

GeneID:4455
TrivialName: PA14_45290
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_45290
PAO1 Ortholog Locus:PA1482
Sequence Length:468
Protein Length:155
Start: 4037211
Stop: 4036744
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04871.1|AE004577_6 (AE004577) cytochrome C-type biogenesis protein CcmH [Pseudomonas aeruginosa PAO1]
Identities = 154/155 (99%), Positives = 155/155 (100%)
Sequence:
ATGAAGCGTTTCCTCGCCACCGCGCTGCTCGGTATCGCCCTCTGCGGCGTGGCCCGGGCCGCCATCGACACC
TACGAGTTCGCCAGCGACGCCGAGCGCGAGCGCTTCCGCAACCTGACCCAGGAACTGCGCTGCCCGAAGTGC
CAGAACCAGGACATCGCCGACTCCAACGCACCGATCGCCGCCGACCTGCGCAAGCAGATCTACGGCCAGTTG
CAGCAGGGCAAGAGCGACGGCGAGATCGTCGACTACATGGTTGCCCGCTACGGTGACTTCGTTCGCTACAAG
CCGCCGGTCAACGAGCGTACCTGGCTGCTCTGGTTCGGTCCGGGCGCCTTGCTGCTGTTCGGCGTGCTGGTG
ATCGGCGTGATCGTCCTGCGCCGCCGGCGCACCGCCGCCAAGGTGCAAACCACCCTGTCCGCCGAGGAGCAA
GCGCGCCTCGCCAACCTGTTGAAGAACGACAAATGA
Translation:
MKRFLATALLGIALCGVARAAIDTYEFASDAERERFRNLTQELRCPKCQNQDIADSNAPIAADLRKQIYGQL
QQGKSDGEIVDYMVARYGDFVRYKPPVNERTWLLWFGPGALLLFGVLVIGVIVLRRRRTAAKVQTTLSAEEQ
ARLANLLKNDK*
AnnotationID:4461GeneID:4455
AnnotatorUID: diggins
Modification Date/Time: 2005-02-24 17:05:22
Gene Name:ccmH
Confidence Code:2
GeneProduct:cytochrome C-type biogenesis protein CcmH
Cell Localization:()
Synonyms:cycL
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(12) Energy metabolism
Alternate Gene Product Name:cytochrome c-type biogenesis protein CycL
Functional Category Confidence Code:5
COGs:COG3088,pfam03918
Secondary Functional Category(ies):central intermediary metabolism
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO57099.1| (AE016869) cytochrome c-type biogenesis protein CycL [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 156

 Score =  201 bits (512), Expect = 9e-52
 Identities = 99/155 (63%), Positives = 118/155 (76%)

>emb|CAA88020.1| (Z47979) periplasmic or inner membrane associated protein
           [Pseudomonas fluorescens]
          Length = 149

 Score =  170 bits (430), Expect = 3e-42
 Identities = 85/129 (65%), Positives = 103/129 (79%), Gaps = 1/129 (0%)

>gb|AAM85143.1|AE013760_8 (AE013760) putative cytochrome c-type biogenesis protein [Yersinia
           pestis KIM]
          Length = 170

 Score =  144 bits (364), Expect = 1e-34
 Identities = 77/147 (52%), Positives = 101/147 (68%), Gaps = 4/147 (2%)








 
Structural Features:
COG3088, CcmH, Uncharacterized protein involved in biosynthesis 
of c-type cytochromes 
[Posttranslational modification, protein turnover, chaperones]
CD-Length = 153 residues,  98.7% aligned
Score =  198 bits (505), Expect = 2e-52
Identities = 86/154 (55%), Positives = 104/154 (67%), Gaps = 3/154 (1%)

pfam03918, CcmH, Cytochrome C biogenesis protein. 
CD-Length = 149 residues,  98.7% aligned
Score =  173 bits (439), Expect = 9e-45
Identities = 75/148 (50%), Positives = 98/148 (66%), Gaps = 1/148 (0%)






   
Genomic Context:






   
Comment:


















    ReferenceID:8911




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:26053




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36499




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:4455_cdd.html


    		




 
URL:

















    ReferenceID:36564




  
Author/Investigator(s):
        Fabianek RA, Hofer T, Thony-Meyer L.





  
Title:
        Characterization of the Escherichia coli CcmH protein reveals new insights into the redox pathway required for cytochrome c maturation.




  
PubMed:
        9914305


MedLine:
        




    
Source:
Arch Microbiol. 1999 Jan;171(2):92-100.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9914305

















    ReferenceID:36565




  
Author/Investigator(s):
        Tanapongpipat S, Reid E, Cole JA, Crooke H.





  
Title:
        Transcriptional control and essential roles of the Escherichia coli ccm gene products in formate-dependent nitrite reduction and cytochrome c synthesis.




  
PubMed:
        9716493


MedLine:
        




    
Source:
Biochem J. 1998 Sep 1;334 ( Pt 2):355-65.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9716493

















    ReferenceID:37952




  
Author/Investigator(s):
        Thony-Meyer L, Fischer F, Kunzler P, Ritz D, Hennecke H.





  
Title:
        Escherichia coli genes required for cytochrome c maturation.




  
PubMed:
        7635817


MedLine:
        




    
Source:
J Bacteriol. 1995 Aug;177(15):4321-6.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=7635817









           
Homologs By Global Alignment 

           

       



     
Gene ID:4455
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6798
                           PA1482_tr
                           translation of PA1482
                           155
                           99.35
                           99.99
                           0
                           788.0
     

     
     
182043
                           gb|AAG04871.1|AE004577_6
                           (AE004577) cytochrome C-type biogenesis protein CcmH [Pseudomonas aeruginosa PAO1]
                           155
                           99.35
                           99.99
                           0
                           788.0
     

     
     
182044
                           gb|AAO57099.1|
                           (AE016869) cytochrome c-type biogenesis protein CycL [Pseudomonas syringae pv. tomato str. DC3000]
                           156
                           63.46
                           75.64
                           1
                           512.0
     

     
     
182045
                           gb|AAC44228.1|
                           (U44827) CycL [Pseudomonas fluorescens]
                           158
                           63.29
                           73.41
                           5
                           476.0
     

     
     
182046
                           emb|CAA88020.1|
                           (Z47979) periplasmic or inner membrane associated protein [Pseudomonas fluorescens]
                           156
                           56.41
                           70.51
                           8
                           426.0
     

     






      

          
GC ORFID: 47457How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1482Glimmer Score: 

          
Start: 916698Stop: 917165

          
Length: 468


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1482 translation of PA1482Homolog Bit Score 308.0

          
Other Homologs:  psyr_15may02_Scaffold13_revised_gene2232 (ORF: BLASTX 193.0), t_PA1482 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAGCGTTTCCTCGCCACCGCGCTGCTCGGTATCGCCCTCTGCGGCGTGGCCCGGGCCGCCATCGACACC 
TACGAGTTCGCCAGCGACGCCGAGCGCGAGCGCTTCCGCAACCTGACCCAGGAACTGCGCTGCCCGAAGTGC 
CAGAACCAGGACATCGCCGACTCCAACGCACCGATCGCCGCCGACCTGCGCAAGCAGATCTACGGCCAGTTG 
CAGCAGGGCAAGAGCGACGGCGAGATCGTCGACTACATGGTTGCCCGCTACGGTGACTTCGTTCGCTACAAG 
CCGCCGGTCAACGAGCGTACCTGGCTGCTCTGGTTCGGTCCGGGCGCCTTGCTGCTGTTCGGCGTGCTGGTG 
ATCGGCGTGATCGTCCTGCGCCGCCGGCGCACCGCCGCCAAGGTGCAAACCACCCTGTCCGCCGAGGAGCAA 
GCGCGCCTCGCCAACCTGTTGAAGAACGACAAATGA