ViewGene
Locus Name:
GeneID:
Location:

GeneID:4152
TrivialName: PA14_42850
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_42850
PAO1 Ortholog Locus:PA1674
Sequence Length:546
Protein Length:181
Start: 3808774
Stop: 3809319
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05063.1|AE004595_2 (AE004595) GTP cyclohydrolase I precursor [Pseudomonas aeruginosa PAO1]
Identities = 181/181 (100%), Positives = 181/181 (100%)
Sequence:
ATGTCCCTGGAACAACACTACTCCTCGATCCTCACCCAGCTCGGCGAGGACGTTAACCGCGAAGGTCTGCTG
GACACGCCCAAGCGTGCCGCCAAGGCCATGAAGTACCTGTGCCGCGGCTACCAACAGAGCCTGGAAGAAGTC
GTCAACGGTGCCCTGTTCAGCTCCGACAACAGCGAAATGGTATTGGTCAAGGACATCGAGCTGTACTCGCTC
TGCGAGCACCACCTGCTGCCCTTCATCGGCAAGGCCCACGTGGCCTATATTCCGAACGGCAAGGTGCTCGGC
CTGTCGAAGGTCGCGCGGATCGTCGACATGTACGCCCGCCGCCTGCAGATCCAGGAGAACATGAGCCGGCAG
ATCGCCGAGGCGGTTCAGCAGGTCACCGGCGCCCTCGGCGTCGCGGTGGTGATCCAGGCCCAGCACATGTGC
ATGATGATGCGCGGCGTGGAGAAGCAGAACTCGTCCATGGTCACTTCGGTGATGCTCGGCGAATTCCGCGAC
AACGCCGCCACCCGCAGCGAATTCCTCAGCCTGATCCGCTGA
Translation:
MSLEQHYSSILTQLGEDVNREGLLDTPKRAAKAMKYLCRGYQQSLEEVVNGALFSSDNSEMVLVKDIELYSL
CEHHLLPFIGKAHVAYIPNGKVLGLSKVARIVDMYARRLQIQENMSRQIAEAVQQVTGALGVAVVIQAQHMC
MMMRGVEKQNSSMVTSVMLGEFRDNAATRSEFLSLIR*
AnnotationID:4159GeneID:4152
AnnotatorUID: diggins
Modification Date/Time: 2005-06-01 07:07:21
Gene Name:folE2
Confidence Code:2
GeneProduct:GTP cyclohydrolase I precursor
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0302,cd00642
Secondary Functional Category(ies):
EC Number:3.5.4.16
Status:ACTIVE
Pathway:
Folate biosynthesis
Homology:
>gb|AAN67442.1|AE016371_8 (AE016780) GTP cyclohydrolase I [Pseudomonas putida KT2440]
          Length = 190

 Score =  321 bits (823), Expect = 1e-87
 Identities = 158/181 (87%), Positives = 176/181 (97%)

>gb|AAO55553.1| (AE016863) GTP cyclohydrolase I [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 181

 Score =  320 bits (819), Expect = 3e-87
 Identities = 160/181 (88%), Positives = 176/181 (97%)

>gb|AAO90329.1| (AE016962) GTP cyclohydrolase I [Coxiella burnetii RSA 493]
          Length = 184

 Score =  241 bits (615), Expect = 2e-63
 Identities = 116/180 (64%), Positives = 146/180 (81%)
Structural Features:
COG0302, FolE, GTP cyclohydrolase I [Coenzyme metabolism]
CD-Length = 195 residues,  92.3% aligned
Score =  285 bits (730), Expect = 3e-78
Identities = 110/180 (61%), Positives = 138/180 (76%)

cd00642, GTP_cyclohydro1, GTP cyclohydrolase I (GTP-CH-I) 
catalyzes the conversion of GTP into dihydroneopterin triphosphate.
CD-Length = 185 residues,  97.3% aligned
Score =  253 bits (648), Expect = 8e-69
Identities = 103/180 (57%), Positives = 134/180 (74%)
Genomic Context:






   
Comment:


















    ReferenceID:8306




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:25449




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37179




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:4152_cdd.html


    		




 
URL:

















    ReferenceID:37876




  
Author/Investigator(s):
        Hatakeyama K, Inoue Y, Harada T, Kagamiyama H.





  
Title:
        Cloning and sequencing of cDNA encoding rat GTP cyclohydrolase I. The first enzyme of the tetrahydrobiopterin biosynthetic pathway.




  
PubMed:
        1985963


MedLine:
        




    
Source:
J Biol Chem. 1991 Jan 15;266(2):765-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=91093270&dopt=Abstract

















    ReferenceID:37877




  
Author/Investigator(s):
        Schoedon G, Redweik U, Frank G, Cotton RG, Blau N.





  
Title:
        Allosteric characteristics of GTP cyclohydrolase I from Escherichia coli.




  
PubMed:
        1459137


MedLine:
        




    
Source:
Eur J Biochem. 1992 Dec 1;210(2):561-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=93092993&dopt=Abstract









           
Homologs By Global Alignment 

           

       



     
Gene ID:4152
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6416
                           PA1674_tr
                           translation of PA1674
                           181
                           99.99
                           99.99
                           0
                           902.0
     

     
     
175670
                           gb|AAG05063.1|AE004595_2
                           (AE004595) GTP cyclohydrolase I precursor [Pseudomonas aeruginosa PAO1]
                           181
                           99.99
                           99.99
                           0
                           902.0
     

     
     
175672
                           gb|AAO55553.1|
                           (AE016863) GTP cyclohydrolase I [Pseudomonas syringae pv. tomato str. DC3000]
                           181
                           88.39
                           97.23
                           0
                           819.0
     

     
     
175671
                           gb|AAN67442.1|AE016371_8
                           (AE016780) GTP cyclohydrolase I [Pseudomonas putida KT2440]
                           190
                           83.15
                           92.63
                           9
                           823.0
     

     
     
6417
                           PA3438_tr
                           translation of PA3438
                           186
                           78.49
                           85.48
                           5
                           743.0
     

     






      

          
GC ORFID: 47154How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1674Glimmer Score: 

          
Start: 1145135Stop: 1144590

          
Length: 546


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1674 translation of PA1674Homolog Bit Score 352.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene561 (ORF: BLASTX 322.0), t_PA3438 (ORF: BLASTX 292.0), psyr_15may02_Scaffold3_revised_gene5034 (ORF: BLASTX 289.0), t_PA1674 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCCCTGGAACAACACTACTCCTCGATCCTCACCCAGCTCGGCGAGGACGTTAACCGCGAAGGTCTGCTG 
GACACGCCCAAGCGTGCCGCCAAGGCCATGAAGTACCTGTGCCGCGGCTACCAACAGAGCCTGGAAGAAGTC 
GTCAACGGTGCCCTGTTCAGCTCCGACAACAGCGAAATGGTATTGGTCAAGGACATCGAGCTGTACTCGCTC 
TGCGAGCACCACCTGCTGCCCTTCATCGGCAAGGCCCACGTGGCCTATATTCCGAACGGCAAGGTGCTCGGC 
CTGTCGAAGGTCGCGCGGATCGTCGACATGTACGCCCGCCGCCTGCAGATCCAGGAGAACATGAGCCGGCAG 
ATCGCCGAGGCGGTTCAGCAGGTCACCGGCGCCCTCGGCGTCGCGGTGGTGATCCAGGCCCAGCACATGTGC 
ATGATGATGCGCGGCGTGGAGAAGCAGAACTCGTCCATGGTCACTTCGGTGATGCTCGGCGAATTCCGCGAC 
AACGCCGCCACCCGCAGCGAATTCCTCAGCCTGATCCGCTGA