ViewGene
Locus Name:
GeneID:
Location:

GeneID:412
TrivialName: PA14_43850
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_43850
PAO1 Ortholog Locus:PA1596
Sequence Length:1905
Protein Length:634
Start: 3905972
Stop: 3904068
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04985.1|AE004587_9 (AE004587) heat shock protein HtpG [Pseudomonas aeruginosa PAO1]
Identities = 634/634 (100%), Positives = 634/634 (100%)
Sequence:
ATGAGCGTGGAAACCCAAAAAGAGACACTGGGCTTTCAGACTGAAGTGAAGCAACTGCTTCACCTGATGATT
CATTCTCTCTATTCGAACAAGGAAATCTTCCTCCGCGAACTGATTTCCAACGCCTCGGACGCCGCCGACAAG
CTGCGTTTCGAAGCCCTGGCCAATCCCGAGCTGCTCGAGGGCGGCGCCGAGCTGAAGATCCGCGTCAGCTTC
GACAAGGAAGCCAACACCGTCACCCTGGAAGACAACGGCATCGGCATGAGCCGCGAGGATGTGGTGACTCAC
CTGGGCACCATCGCCAAGTCCGGGACCGCCGACTTCCTGAAGAACTTGTCTGGCGACCAGAAGAAGGATTCG
CACCTGATCGGCCAGTTCGGCGTCGGTTTCTACAGCGCCTTCATCGTCGCCGACAAGGTCGATGTCTACTCC
CGTCGCGCCGGCCAGCCGGCCAGCGAAGGCGTGCACTGGTCGTCGAAGGGCGAGGGCGAGTTCGACGTCGCC
ACCATCGACAAGCCCGAGCGCGGCACCCGCATCGTCCTCCACCTGAAAAAGGGCGAGGAGGAGTTCGCCGAT
GGCTGGCGCCTGCGCAACGTGATCAAGAAATACTCCGATCACATCGCTTTGCCGATCGAGCTACCCAAGGAA
TTCCACGGCGAAGAGGCCGACAAGCCGGCCGAGCCGGAATGGGAAACGGTCAACCGCGCCAGCGCGCTGTGG
ACCCGTCCGCGCGCCGAGGTGAAGGACGAGGAATACCAGGAGTTCTACAAGCACGTCGCCCATGACTTCGAG
AACCCGCTGAGCTGGAGCCACAACAAGGTCGAAGGCAAGCTGGAATACACCTCGCTGCTCTACGTGCCGGGC
CGCGCGCCGTTCGACCTGTACCATCGCGAGGCGCCACGAGGCCTGAAGCTGTACGTACAGCGCGTGTTCATC
ATGGACCAGGCCGACGAGTTCCTGCCGCTGTACCTGCGCTTCATCAAGGGTGTGGTCGACTCCAACGACCTG
TCGCTGAACGTCTCCCGGGAAATCCTGCAGAAGGATCCGGTGATCGACTCGATGAAGTCGGCGTTGACCAAG
CGCGTGCTGGACATGCTGGAGAAACTGGCGAAGAACGAGCCGGAGCAGTACAAGACCTTCTGGAAGAACTTC
GGCCAGGTCCTCAAGGAAGGCCCGGCGGAAGACTTCGGCAACAAGGAGAAGATCGCCGGCCTGCTGCGCTTC
GCCTCCACCGGCGACGATTCGGGCGAGCAGAGCGTAGCGCTGGCCGACTACATCGGTCGGATGAAGGAAGGC
CAGGACAAGATCTACTACCTCACCGGCGAGAGCTACTCGCAGGTGAAGAACAGCCCGCACCTGGAAGTCTTC
CGCAAGAAGGGCATCGAGGTGCTGCTGCTCACCGATCGCATCGACGAGTGGCTGATGAGCTACCTGCCGGAG
TTCGACGGCAAGCAGTTCGTCGATGTCGCCCGCGGCGACCTGGACCTGGGCAGCCTGGACTCGGAAGAGGAC
AAGAAGGCCCAGGAAGAAGTCGCCAAGAGCAAGGAAGGCCTGATCGAGCGCCTGAAGAAGGTGCTCGACGAG
CAGGTCAGCGAAGTCCGCGTCTCCCACCGCCTGACCGATTCGCCGGCGATCCTCGCCATCGGCGAACAGGAC
CTTGGCCTGCAGATGCGCCAGATCCTCGAGGCCAGTGGGCAGAAAGTGCCGGACTCGAAGCCGATCTTCGAG
ATCAACCCGCAGCATCCGCTGATCGAGAAGCTGGATGCGGAGCCGGACGAGGACCGCTTTGGCGAGCTGTCG
CATATTCTCTTCGACCAGGCCGCCCTGGCCGCGGGGGACAGCCTCAAGGATCCGGGCGCCTACGTACGCCGA
CTGAACAAGCTGCTGGTGGAGCTGTCCGCTTAA
Translation:
MSVETQKETLGFQTEVKQLLHLMIHSLYSNKEIFLRELISNASDAADKLRFEALANPELLEGGAELKIRVSF
DKEANTVTLEDNGIGMSREDVVTHLGTIAKSGTADFLKNLSGDQKKDSHLIGQFGVGFYSAFIVADKVDVYS
RRAGQPASEGVHWSSKGEGEFDVATIDKPERGTRIVLHLKKGEEEFADGWRLRNVIKKYSDHIALPIELPKE
FHGEEADKPAEPEWETVNRASALWTRPRAEVKDEEYQEFYKHVAHDFENPLSWSHNKVEGKLEYTSLLYVPG
RAPFDLYHREAPRGLKLYVQRVFIMDQADEFLPLYLRFIKGVVDSNDLSLNVSREILQKDPVIDSMKSALTK
RVLDMLEKLAKNEPEQYKTFWKNFGQVLKEGPAEDFGNKEKIAGLLRFASTGDDSGEQSVALADYIGRMKEG
QDKIYYLTGESYSQVKNSPHLEVFRKKGIEVLLLTDRIDEWLMSYLPEFDGKQFVDVARGDLDLGSLDSEED
KKAQEEVAKSKEGLIERLKKVLDEQVSEVRVSHRLTDSPAILAIGEQDLGLQMRQILEASGQKVPDSKPIFE
INPQHPLIEKLDAEPDEDRFGELSHILFDQAALAAGDSLKDPGAYVRRLNKLLVELSA*
AnnotationID:421GeneID:412
AnnotatorUID: diggins
Modification Date/Time: 2005-02-25 15:03:07
Gene Name:htpG
Confidence Code:2
GeneProduct:heat shock protein HtpG
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(9) Chaperones & heat shock proteins
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0326,pfam00183
Secondary Functional Category(ies):posttranslational modification, protein turnover, chaperones
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO55724.1| (AE016863) heat shock protein HtpG [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 635

 Score = 1129 bits (2921), Expect = 0.0
 Identities = 570/637 (89%), Positives = 599/637 (94%), Gaps = 7/637 (1%)

>gb|AAN69760.1|AE016612_6 (AE016789) heat shock protein HtpG [Pseudomonas putida KT2440]
          Length = 634

 Score = 1128 bits (2918), Expect = 0.0
 Identities = 564/634 (88%), Positives = 592/634 (93%)

>gb|AAN79071.1|AE016756_254 (AE016756) Chaperone protein htpG [Escherichia coli CFT073]
          Length = 624

 Score =  784 bits (2024), Expect = 0.0
 Identities = 382/624 (61%), Positives = 490/624 (78%), Gaps = 4/624 (0%)








 
Structural Features:
COG0326, HtpG, Molecular chaperone, HSP90 family 
[Posttranslational modification, protein turnover, chaperones]
CD-Length = 623 residues, 100.0% aligned
Score =  859 bits (2222), Expect = 0.0
Identities = 385/632 (60%), Positives = 492/632 (77%), Gaps = 10/632 (1%)

pfam00183, HSP90, Hsp90 protein.
CD-Length = 529 residues,  92.6% aligned
Score =  365 bits (939), Expect = 7e-102
Identities = 193/495 (38%), Positives = 294/495 (59%), Gaps = 59/495 (11%)










   
Genomic Context:






   
Comment:


















    ReferenceID:827




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17969




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37246




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:412_cdd.html


    		




 
URL:

















    ReferenceID:37902




  
Author/Investigator(s):
        Yoshida K, Seki S, Fujimura M, Miwa Y, Fujita Y.





  
Title:
        Cloning and sequencing of a 36-kb region of the Bacillus subtilis genome between the gnt and iol operons.




  
PubMed:
        7584049


MedLine:
        




    
Source:
DNA Res. 1995;2(2):61-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7584049

















    ReferenceID:37903




  
Author/Investigator(s):
        Spence J, Georgopoulos C.





  
Title:
        Purification and properties of the Escherichia coli heat shock protein, HtpG.




  
PubMed:
        2647735


MedLine:
        




    
Source:
J Biol Chem. 1989 Mar 15;264(8):4398-403.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2647735

















    ReferenceID:37904




  
Author/Investigator(s):
        Tanaka N, Nakamoto H.





  
Title:
        HtpG is essential for the thermal stress management in cyanobacteria.




  
PubMed:
        10481048


MedLine:
        




    
Source:
FEBS Lett. 1999 Sep 17;458(2):117-23.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10481048









           
Homologs By Global Alignment 

           

       



     
Gene ID:412
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
698
                           PA1596_tr
                           translation of PA1596
                           634
                           99.99
                           99.99
                           0
                           3251.0
     

     
     
31615
                           gb|AAG04985.1|AE004587_9
                           (AE004587) heat shock protein HtpG [Pseudomonas aeruginosa PAO1]
                           634
                           99.99
                           99.99
                           0
                           3251.0
     

     
     
31616
                           gb|AAO55724.1|
                           (AE016863) heat shock protein HtpG [Pseudomonas syringae pv. tomato str. DC3000]
                           638
                           89.34
                           93.88
                           7
                           2929.0
     

     
     
31617
                           gb|AAN69760.1|AE016612_6
                           (AE016789) heat shock protein HtpG [Pseudomonas putida KT2440]
                           637
                           89.32
                           93.56
                           6
                           2919.0
     

     
     
31618
                           gb|AAM37379.1|
                           (AE011891) heat shock protein G [Xanthomonas axonopodis pv. citri str. 306]
                           634
                           82.33
                           92.11
                           0
                           2771.0
     

     






      

          
GC ORFID: 43414How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1596Glimmer Score: 

          
Start: 1047937Stop: 1049841

          
Length: 1905


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA1596 translation of PA1596Homolog Bit Score 1256.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene1003 (ORF: BLASTX 1133.0), t_PA1596 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCGTGGAAACCCAAAAAGAGACACTGGGCTTTCAGACTGAAGTGAAGCAACTGCTTCACCTGATGATT 
CATTCTCTCTATTCGAACAAGGAAATCTTCCTCCGCGAACTGATTTCCAACGCCTCGGACGCCGCCGACAAG 
CTGCGTTTCGAAGCCCTGGCCAATCCCGAGCTGCTCGAGGGCGGCGCCGAGCTGAAGATCCGCGTCAGCTTC 
GACAAGGAAGCCAACACCGTCACCCTGGAAGACAACGGCATCGGCATGAGCCGCGAGGATGTGGTGACTCAC 
CTGGGCACCATCGCCAAGTCCGGGACCGCCGACTTCCTGAAGAACTTGTCTGGCGACCAGAAGAAGGATTCG 
CACCTGATCGGCCAGTTCGGCGTCGGTTTCTACAGCGCCTTCATCGTCGCCGACAAGGTCGATGTCTACTCC 
CGTCGCGCCGGCCAGCCGGCCAGCGAAGGCGTGCACTGGTCGTCGAAGGGCGAGGGCGAGTTCGACGTCGCC 
ACCATCGACAAGCCCGAGCGCGGCACCCGCATCGTCCTCCACCTGAAAAAGGGCGAGGAGGAGTTCGCCGAT 
GGCTGGCGCCTGCGCAACGTGATCAAGAAATACTCCGATCACATCGCTTTGCCGATCGAGCTACCCAAGGAA 
TTCCACGGCGAAGAGGCCGACAAGCCGGCCGAGCCGGAATGGGAAACGGTCAACCGCGCCAGCGCGCTGTGG 
ACCCGTCCGCGCGCCGAGGTGAAGGACGAGGAATACCAGGAGTTCTACAAGCACGTCGCCCATGACTTCGAG 
AACCCGCTGAGCTGGAGCCACAACAAGGTCGAAGGCAAGCTGGAATACACCTCGCTGCTCTACGTGCCGGGC 
CGCGCGCCGTTCGACCTGTACCATCGCGAGGCGCCACGAGGCCTGAAGCTGTACGTACAGCGCGTGTTCATC 
ATGGACCAGGCCGACGAGTTCCTGCCGCTGTACCTGCGCTTCATCAAGGGTGTGGTCGACTCCAACGACCTG 
TCGCTGAACGTCTCCCGGGAAATCCTGCAGAAGGATCCGGTGATCGACTCGATGAAGTCGGCGTTGACCAAG 
CGCGTGCTGGACATGCTGGAGAAACTGGCGAAGAACGAGCCGGAGCAGTACAAGACCTTCTGGAAGAACTTC 
GGCCAGGTCCTCAAGGAAGGCCCGGCGGAAGACTTCGGCAACAAGGAGAAGATCGCCGGCCTGCTGCGCTTC 
GCCTCCACCGGCGACGATTCGGGCGAGCAGAGCGTAGCGCTGGCCGACTACATCGGTCGGATGAAGGAAGGC 
CAGGACAAGATCTACTACCTCACCGGCGAGAGCTACTCGCAGGTGAAGAACAGCCCGCACCTGGAAGTCTTC 
CGCAAGAAGGGCATCGAGGTGCTGCTGCTCACCGATCGCATCGACGAGTGGCTGATGAGCTACCTGCCGGAG 
TTCGACGGCAAGCAGTTCGTCGATGTCGCCCGCGGCGACCTGGACCTGGGCAGCCTGGACTCGGAAGAGGAC 
AAGAAGGCCCAGGAAGAAGTCGCCAAGAGCAAGGAAGGCCTGATCGAGCGCCTGAAGAAGGTGCTCGACGAG 
CAGGTCAGCGAAGTCCGCGTCTCCCACCGCCTGACCGATTCGCCGGCGATCCTCGCCATCGGCGAACAGGAC 
CTTGGCCTGCAGATGCGCCAGATCCTCGAGGCCAGTGGGCAGAAAGTGCCGGACTCGAAGCCGATCTTCGAG 
ATCAACCCGCAGCATCCGCTGATCGAGAAGCTGGATGCGGAGCCGGACGAGGACCGCTTTGGCGAGCTGTCG 
CATATTCTCTTCGACCAGGCCGCCCTGGCCGCGGGGGACAGCCTCAAGGATCCGGGCGCCTACGTACGCCGA 
CTGAACAAGCTGCTGGTGGAGCTGTCCGCTTAA