ViewGene
Locus Name:
GeneID:
Location:

GeneID:4086
TrivialName: PA14_35890
AnnotatorUID: ericdeziel
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_35890
PAO1 Ortholog Locus:
Sequence Length:1254
Protein Length:417
Start: 3193841
Stop: 3195094
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAK01505.1| (AF241171) diaminobutyric acid aminotransferase [Pseudomonas aeruginosa]
Identities = 417/417 (100%), Positives = 417/417 (100%) to gene #15 of PAGI-1 island.
Sequence:
ATGAACAACATCGAGACGTTCGAAAGGCTCGAGTCCAACGTGCGGATGTACTGCCGCGACTTGCCCCTGGTG
TTCGATACCGCCCAAGGCAGCAGGATCCGCGGCGAAGATGGCCGCGAATACCTGGACTTCTTCGCCGGCGCC
GGCGCCCTGAACTATGGCCACAACGACCCGCGCATGCGCGATGCGCTGATCGCGTATCTGTCGGCCAACGGC
GTGACCCACGCCCTCGACCTGCACACCACCACCAAGCGCCAGTTCCTGGAAGCGATCGAGCGCGACCTGTTC
AAGCCACGCGGCTGGGACTACAAGGTCCAGTTCACCGGCCCGACCGGTGCCGACGCCGTCGAGGCGGCGTTG
AAGCTGGCGCGCAAGGCGACCGGGCGTACCAAGGTCGTATCGTTCTACGGGGCTTACCACGGCATGACCGCC
GGCGCCCTGGCCGTCACCGGCAACCGCACTCGCCGTGGCCCCGGGCTTTCCACCGACGTGGTGTTCGTGCCC
TACGAAGACAGCCCCTATGGCGAATTCGACAGCATTGGCTTCCTCGAGCGCCTGGCCAACGACCAGGGCAGC
GGCTCGGAGCTGCCGGCGGCGGTGATCGTCGAGTCGGTGCAGATCCAGGCCGGCGTCTACCCGGCTTCCAAC
CAATGGCTGCAACGCTTGCGCAAGTGGACCACCGACCACGGCGTGCTGCTTATCTGCGACGAGATCCAGGCC
GGTTGCGGCCGCACCGGCGACTTCTTCGGCTTCGAGCGCTCCGGGGTGGTGCCGGACCTGATCACCTGCGCC
AAATCGATCGGCGGCTTCGGCCTGCCGATGGCGATCGTGTTGCTTCGTGCCGGGCTGGATGTATGGCAACCG
GGCGATCACATCGGCACCTTCCGGGGCAACCAGTTGGCGTTCCTGACCGCCCGCATCGCGCTCAGCTATTGG
CACGATGAGCAGTTCCTGAAATTGCTGGCCGACAACTGCGCGGCCATGGAGCGCGCGGTTGCCGGCTTTGCG
GAAATTCCAGGCGTCGCGTCCGCGCGTTGTCGCGGCATGATCGCCGGCATCGATTTCGGCCGCGGCAACGTC
GAGGTCGCCAAGGACGCCCAGCAGCGCGTGCTGCAAGCGGGCGTGCTGGTCGACCGCTGCGGCCCGAATGGC
GAAATCATCAAGCTGATGCCACCGCTGAACACTCCGACCGACCAACTCGAAGACGGCCTCAGGGCCTTCGGC
GAATCGGTCGCGGCAGCGCTGGGCGAATAG
Translation:
MNNIETFERLESNVRMYCRDLPLVFDTAQGSRIRGEDGREYLDFFAGAGALNYGHNDPRMRDALIAYLSANG
VTHALDLHTTTKRQFLEAIERDLFKPRGWDYKVQFTGPTGADAVEAALKLARKATGRTKVVSFYGAYHGMTA
GALAVTGNRTRRGPGLSTDVVFVPYEDSPYGEFDSIGFLERLANDQGSGSELPAAVIVESVQIQAGVYPASN
QWLQRLRKWTTDHGVLLICDEIQAGCGRTGDFFGFERSGVVPDLITCAKSIGGFGLPMAIVLLRAGLDVWQP
GDHIGTFRGNQLAFLTARIALSYWHDEQFLKLLADNCAAMERAVAGFAEIPGVASARCRGMIAGIDFGRGNV
EVAKDAQQRVLQAGVLVDRCGPNGEIIKLMPPLNTPTDQLEDGLRAFGESVAAALGE*
AnnotationID:4093GeneID:4086
AnnotatorUID: danlee
Modification Date/Time: 2005-10-11 17:05:05
Gene Name:PAGI-1(15)
Confidence Code:3
GeneProduct:Putative aminotransferase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(20) Putative enzymes
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0160, COG4992, pfam00202
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
gi|12698391|gb|AAK01505.1|  diaminobutyric acid aminotransferase [Pseudomonas aeruginosa]
     Length=417
     Score =  843 bits (2179),  Expect = 0.0
     Identities = 417/417 (100%), Positives = 417/417 (100%), Gaps = 0/417 (0%)

gi|24984392|gb|AAN68408.1|  diaminobutyrate-2-oxoglutarate transaminase, putative [Pseudomonas 
putida KT2440]
     Length=417
     Score =  793 bits (2047),  Expect = 0.0
     Identities = 390/415 (93%), Positives = 401/415 (96%), Gaps = 0/415 (0%)






 
Structural Features:
COG0160, GabT, 4-aminobutyrate aminotransferase and related aminotransferases [Amino acid 
transport and metabolism].
             CD-Length = 447 residues,  94.9% aligned
             Score =  347 bits (893), Expect = 1e-96

COG4992, ArgD, Ornithine/acetylornithine aminotransferase [Amino acid transport and metabolism].
             CD-Length = 404 residues,  97.8% aligned
             Score =  264 bits (677), Expect = 1e-71

pfam00202, Aminotran_3, Aminotransferase class-III..
             CD-Length = 402 residues,  99.0% aligned
             Score =  262 bits (670), Expect = 7e-71






   
Genomic Context:
Predicted gene #15 of the PAGI-1 island absent in PAO1 but present in a majority of pathogenic P. aeruginosa isolates.   
The PA14 island at this locus, from PA14_35860 to PA14_36030, corresponds to PAGI-1 genes 17 through 2, respectively.






   
Comment:
The first half of the PA14 island (from PA14_35700 through PA14_35850) diverges from PAGI-1 such that 
genes 18 through 51 are absent.  PA14 has 31 ORFs in 35368 bp (from PA14_35700 to PA14_36030), 
whereas PAGI-1 contains 51 ORFs in 51300 bp


















    ReferenceID:8174




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:25317




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:38205




  
Author/Investigator(s):
        Liang X, Pham XQ, Olson MV, Lory S.





  
Title:
        Identification of a genomic island present in the majority of pathogenic isolates of Pseudomonas aeruginosa.




  
PubMed:
        11208781


MedLine:
        




    
Source:
J Bacteriol. 2001 Feb;183(3):843-53.







    
Reference Type:Journal 







    
Data:


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:4086
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
173988
                           gb|AAK01505.1|
                           (AF241171) diaminobutyric acid aminotransferase [Pseudomonas aeruginosa]
                           417
                           99.99
                           99.99
                           0
                           2179.0
     

     
     
173989
                           gb|AAN68408.1|AE016473_6
                           (AE016784) diaminobutyrate-2-oxoglutarate transaminase, putative [Pseudomonas putida KT2440]
                           417
                           93.52
                           96.40
                           0
                           2047.0
     

     
     
173990
                           dbj|BAB04638.1|
                           (AP001510) diaminobutyric acid aminotransferase [Bacillus halodurans]
                           432
                           46.06
                           63.42
                           20
                           1006.0
     

     
     
173995
                           dbj|BAC74108.1|
                           (AP005046) putative aminotransferase [Streptomyces avermitilis MA-4680]
                           436
                           45.87
                           61.00
                           32
                           954.0
     

     
     
173998
                           emb|CAC38800.1|
                           (AL939110) putative aminotransferase [Streptomyces coelicolor A3(2)]
                           436
                           45.41
                           60.77
                           32
                           943.0
     

     






      

          
GC ORFID: 47088How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: psyr_15may02_Scaffold1_revised_gene915Glimmer Score: 

          
Start: 1760075Stop: 1758822

          
Length: 1254


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: psyr_15may02_Scaffold1_revised_gene915 related to: gi|15613482|ref|NP_241785.1| (NC_002570) diaminobutyric acid aminotransferase [Bacillus halodurans] dbj|BAB04638.1| (AP001510) diaminobutyric acid aminotransferase [Bacillus halodurans]Homolog Bit Score 362.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene915 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAACAACATCGAGACGTTCGAAAGGCTCGAGTCCAACGTGCGGATGTACTGCCGCGACTTGCCCCTGGTG 
TTCGATACCGCCCAAGGCAGCAGGATCCGCGGCGAAGATGGCCGCGAATACCTGGACTTCTTCGCCGGCGCC 
GGCGCCCTGAACTATGGCCACAACGACCCGCGCATGCGCGATGCGCTGATCGCGTATCTGTCGGCCAACGGC 
GTGACCCACGCCCTCGACCTGCACACCACCACCAAGCGCCAGTTCCTGGAAGCGATCGAGCGCGACCTGTTC 
AAGCCACGCGGCTGGGACTACAAGGTCCAGTTCACCGGCCCGACCGGTGCCGACGCCGTCGAGGCGGCGTTG 
AAGCTGGCGCGCAAGGCGACCGGGCGTACCAAGGTCGTATCGTTCTACGGGGCTTACCACGGCATGACCGCC 
GGCGCCCTGGCCGTCACCGGCAACCGCACTCGCCGTGGCCCCGGGCTTTCCACCGACGTGGTGTTCGTGCCC 
TACGAAGACAGCCCCTATGGCGAATTCGACAGCATTGGCTTCCTCGAGCGCCTGGCCAACGACCAGGGCAGC 
GGCTCGGAGCTGCCGGCGGCGGTGATCGTCGAGTCGGTGCAGATCCAGGCCGGCGTCTACCCGGCTTCCAAC 
CAATGGCTGCAACGCTTGCGCAAGTGGACCACCGACCACGGCGTGCTGCTTATCTGCGACGAGATCCAGGCC 
GGTTGCGGCCGCACCGGCGACTTCTTCGGCTTCGAGCGCTCCGGGGTGGTGCCGGACCTGATCACCTGCGCC 
AAATCGATCGGCGGCTTCGGCCTGCCGATGGCGATCGTGTTGCTTCGTGCCGGGCTGGATGTATGGCAACCG 
GGCGATCACATCGGCACCTTCCGGGGCAACCAGTTGGCGTTCCTGACCGCCCGCATCGCGCTCAGCTATTGG 
CACGATGAGCAGTTCCTGAAATTGCTGGCCGACAACTGCGCGGCCATGGAGCGCGCGGTTGCCGGCTTTGCG 
GAAATTCCAGGCGTCGCGTCCGCGCGTTGTCGCGGCATGATCGCCGGCATCGATTTCGGCCGCGGCAACGTC 
GAGGTCGCCAAGGACGCCCAGCAGCGCGTGCTGCAAGCGGGCGTGCTGGTCGACCGCTGCGGCCCGAATGGC 
GAAATCATCAAGCTGATGCCACCGCTGAACACTCCGACCGACCAACTCGAAGACGGCCTCAGGGCCTTCGGC 
GAATCGGTCGCGGCAGCGCTGGGCGAATAG