ViewGene
Locus Name:
GeneID:
Location:

GeneID:3927
TrivialName: PA14_38400
AnnotatorUID: danlee
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_38400
PAO1 Ortholog Locus:
Sequence Length:1179
Protein Length:392
Start: 3421697
Stop: 3422876
Strand: +
Type:
ChromosomeID:2
Status: INACTIVE
Frame Discrepancy: TRUE
Missense Discrepancy:TRUE
Comments:
Inactivated 9-26-05. Replaced by 7358 (which combined 5866 and 3927). Homology: gb|AAC64521.1| (AF073776) MexG [Pseudomonas aeruginosa]
Identities = 216/238 (90%), Positives = 218/238 (91%), Gaps = 2/238 (0%)
Sequence:
ATGGACCGGCTCGCTGCGCGGGCTGCTGGCGGCCCTGGTCGCCCTATTCCTGCTGGGCTGCGAAGAAGCAGC
GGACACCGGGAAGACTGCGGAGGCCCCCGCCGAGGTCGGCGTGATCGTCGCCAGGCCGGCGCCCATCGGCAT
CACCAGCGAGCTGCCCGGACGCCTGGAAGCGTACCGCCAGGCCGAGGTGCGGGCGCGCGTCGCCGGCATCGT
CACCCGTCGCCTGTACGaggAaggccagGACGTCCGCGCCGGCACCGTGCTGTTCcaGATCGACCCGGCGCC
CTTgaaggcgGCCCTGGACATCAGCCGCGGCGCCCTGGCCCGGGCcgaggcCAGCCACGCGGCGGCGGCCGA
CAAGCTCAAGCGCTACGCCGACCTGATCAagGACCGCGCCATCAGCGAACGCGAGTACACCGAAGCGCAGAC
CGACGCGCGCCAGGCCCTGGCGCAGATCGCCTCGgCcaagGCCGAACtggagcAgGCCCGCCTGCGCCTGGG
CTACGCCACGGTCACCGCGCCGATCGACGGCCGCGCGCGGCGCGCGCTGGTcacCGaagcgcgctggtcGGc
gaggacTcGCCGACACCGCTGACCCGCGTCGAGCAGATCGATCCGATCTACGTGAACTTCTCCCAGCCGGCC
GGCGAAGTCGCCGCCATGCAGCGGGCGATCCGCGAAGGCCAGGTGAAGGGTGTCGCCGACAAGGACATCGCC
GTGCGCCTGGTCCTGGCCGACGGCAGCGAGTACCCGCTGGCCGGCGAGCTGCTGTTCTCCGACCTCGCGGTC
GACCCCGGCACCGACACCATCGCCATGCGTGCCCTGTTCCGCAATCCGCATCGCGAATTGCTGCCCGGCGGC
TACGTGCAGGTGCGCCTGCAGCGCGCGGTGAACCCGCAGGCGATCACCGTCCCGCGCGACGCGCTGATCCGT
ACCGCCCAGTCCGCCGTGGTCAAGGTGGTCAACCCACAGGGCGTGGTGGAAGACGTGGAGGTCCGCGCCGAC
ACCCTGCAGGGCCGCGACTGGATCATCAGCCGCGGGCTCAAGGGCGGCGAGCGGGTGATCGTCGAGAACGCC
GcccagcaTGCCGCCGGCTCCAGCGTCCAGGCGGTGGTCCGCCAGCCGGCCAGCGCCGACGCCCCCTCACCG
CTGGCCGCCTCGCCGGCGGGCCAGTGA
Translation:
MDRLAARAAGGPGRPIPAGLRRSSGHREDCGGPRRGRRDRRQAGAHRHHQRAARTPGSVPPGRGAGARRRHR
HPSPVRGRPGRPRRHRAVPDRPGALEGGPGHQPRRPGPGRGQPRGGGRQAQALRRPDQGPRHQRTRVHRSAD
RRAPGPGADRLGQGRTGAGPPAPGLRHGHRADRRPRAARAGHRSALVGEDSPTPLTRVEQIDPIYVNFSQPA
GEVAAMQRAIREGQVKGVADKDIAVRLVLADGSEYPLAGELLFSDLAVDPGTDTIAMRALFRNPHRELLPGG
YVQVRLQRAVNPQAITVPRDALIRTAQSAVVKVVNPQGVVEDVEVRADTLQGRDWIISRGLKGGERVIVENA
AQHAAGSSVQAVVRQPASADAPSPLAASPAGQ*
AnnotationID:3934GeneID:3927
AnnotatorUID: danlee
Modification Date/Time: 2005-09-26 14:02:17
Gene Name:amrA
Confidence Code:
GeneProduct:putative membrane fusion protein
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:()
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:
Secondary Functional Category(ies):transport of small molecules
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAC27753.1| (AF072887) putative membrane fusion protein AmrA [Burkholderia
           pseudomallei]
          Length = 399

 Score =  237 bits (604), Expect = 1e-61
 Identities = 120/204 (58%), Positives = 151/204 (74%)

>emb|CAD85024.1| (BX321859) HlyD family secretion protein [Nitrosomonas europaea
           ATCC 19718]
          Length = 386

 Score =  224 bits (570), Expect = 9e-58
 Identities = 109/185 (58%), Positives = 142/185 (76%)








 
Structural Features:






   
Genomic Context:






   
Comment:
possible frameshift; see gene #5866


















    ReferenceID:7857




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:24999




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32677




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3927_cdd.html


    		




 
URL:

















    ReferenceID:34388




  
Author/Investigator(s):
        Wang W, Zhang W, Chen H, Chiao J, Zhao G, Jiang W.





  
Title:
        Molecular and biochemical characterization of a novel two-component signal transduction system, amrA- amkA, involved in rifamycin SV production in Amycolatopsis mediterranei U32.




  
PubMed:
        12375106


MedLine:
        




    
Source:
Arch Microbiol. 2002 Nov;178(5):376-86. Epub 2002 Aug 22.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12375106

















    ReferenceID:34389




  
Author/Investigator(s):
        Ribardo DA, McIver KS.





  
Title:
        amrA encodes a putative membrane protein necessary for maximal exponential phase expression of the Mga virulence regulon in Streptococcus pyogenes.




  
PubMed:
        14617188


MedLine:
        




    
Source:
Mol Microbiol. 2003 Oct;50(2):673-85.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=14617188









           
Homologs By Global Alignment 

           

       



     
Gene ID:3927
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
171071
                           gb|AAC64521.1|
                           (AF073776) MexG [Pseudomonas aeruginosa]
                           438
                           57.99
                           61.64
                           88
                           1048.0
     

     
     
6166
                           PA2019_tr
                           translation of PA2019
                           452
                           54.20
                           58.62
                           116
                           994.0
     

     
     
171072
                           gb|AAG05407.1|AE004628_5
                           (AE004628) RND multidrug efflux membrane fusion protein precursor [Pseudomonas aeruginosa PAO1]
                           452
                           54.20
                           58.62
                           116
                           994.0
     

     
     
171073
                           gb|AAF14522.1|AF147719_2
                           (AF147719) putative membrane fusion protein; AmrA [Pseudomonas aeruginosa]
                           452
                           54.20
                           58.62
                           116
                           994.0
     

     
     
171074
                           dbj|BAA34299.1|
                           (AB015853) MexX [Pseudomonas aeruginosa]
                           451
                           48.78
                           53.65
                           121
                           833.0
     

     






      

          
GC ORFID: 46929How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2019Glimmer Score: 

          
Start: 1532213Stop: 1531035

          
Length: 1179


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2019 translation of PA2019Homolog Bit Score 389.0

          
Other Homologs:  t_PA2019 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGACCGGCTCGCTGCGCGGGCTGCTGGCGGCCCTGGTCGCCCTATTCCTGCTGGGCTGCGAAGAAGCAGC 
GGACACCGGGAAGACTGCGGAGGCCCCCGCCGAGGTCGGCGTGATCGTCGCCAGGCCGGCGCCCATCGGCAT 
CACCAGCGAGCTGCCCGGACGCCTGGAAGCGTACCGCCAGGCCGAGGTGCGGGCGCGCGTCGCCGGCATCGT 
CACCCGTCGCCTGTACGaggAaggccagGACGTCCGCGCCGGCACCGTGCTGTTCcaGATCGACCCGGCGCC 
CTTgaaggcgGCCCTGGACATCAGCCGCGGCGCCCTGGCCCGGGCcgaggcCAGCCACGCGGCGGCGGCCGA 
CAAGCTCAAGCGCTACGCCGACCTGATCAagGACCGCGCCATCAGCGAACGCGAGTACACCGAAGCGCAGAC 
CGACGCGCGCCAGGCCCTGGCGCAGATCGCCTCGgCcaagGCCGAACtggagcAgGCCCGCCTGCGCCTGGG 
CTACGCCACGGTCACCGCGCCGATCGACGGCCGCGCGCGGCGCGCGCTGGTcacCGaagcgcgctggtcGGc 
gaggacTcGCCGACACCGCTGACCCGCGTCGAGCAGATCGATCCGATCTACGTGAACTTCTCCCAGCCGGCC 
GGCGAAGTCGCCGCCATGCAGCGGGCGATCCGCGAAGGCCAGGTGAAGGGTGTCGCCGACAAGGACATCGCC 
GTGCGCCTGGTCCTGGCCGACGGCAGCGAGTACCCGCTGGCCGGCGAGCTGCTGTTCTCCGACCTCGCGGTC 
GACCCCGGCACCGACACCATCGCCATGCGTGCCCTGTTCCGCAATCCGCATCGCGAATTGCTGCCCGGCGGC 
TACGTGCAGGTGCGCCTGCAGCGCGCGGTGAACCCGCAGGCGATCACCGTCCCGCGCGACGCGCTGATCCGT 
ACCGCCCAGTCCGCCGTGGTCAAGGTGGTCAACCCACAGGGCGTGGTGGAAGACGTGGAGGTCCGCGCCGAC 
ACCCTGCAGGGCCGCGACTGGATCATCAGCCGCGGGCTCAAGGGCGGCGAGCGGGTGATCGTCGAGAACGCC 
GcccagcaTGCCGCCGGCTCCAGCGTCCAGGCGGTGGTCCGCCAGCCGGCCAGCGCCGACGCCCCCTCACCG 
CTGGCCGCCTCGCCGGCGGGCCAGTGA