ViewGene
Locus Name:
GeneID:
Location:

GeneID:385
TrivialName: PA14_18150
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_18150
PAO1 Ortholog Locus:PA3568
Sequence Length:1887
Protein Length:628
Start: 1557036
Stop: 1558922
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06956.1|AE004777_9 (AE004777) probable acetyl-coa synthetase [Pseudomonas aeruginosa PAO1]
Identities = 625/628 (99%), Positives = 625/628 (99%)
Sequence:
ATGACGTACCTGCAGCAGCACGCCCGCTCGATCGCCGAGCCGGCCGCGTTCTGGGCCGAACAGGCCCGTTCC
CTGGCCTGGTACCAGGCGCCCGCGAATATCCTCGAAAGCCTGCCCGACGGCACCCATCGCTGGTTCGCCGAT
GGCCGCCTGAACAGCGCCTACCTGGCCCTCGACCGGCAGATCGAGGAGGGGCGCGGCGAACAGACCGCGCTG
ATCTACGACTCGCCGGTGACCGGCACCCAGGACCGCTACAGCTACCTGCGGCTGCGCGACGAAGTGGCGCGC
CTGGCCGGCGCCCTGCGGGCCCTCGGGGTCGGCAAGGGCGACCGGGTGATCATCTACATGCCGATGGTGCCG
CAGGCGGCCATGGCGATGCTCGCTTGCGCGCGCCTGGGCGCGGTGCATTCGGTGGTGTTCGGTGGCTTCGCT
CCCTACGAGCTGGCCCTGCGCATCGACGACGCCACGCCGAAGCTGGTGCTCACCGCTTCCTGCGGGCTGGAG
TTCGACCGGGTGATCGAATACAAGCCGCTGGTGGACAAGGCCCTCGAACTGGCGATCCACCAGCCTGCCCAC
GTCATGGTCTGGCAACGCCCGCAGGCGCTCGCGCGCCTGCACCCGGGCCGCGACCTGGACTGGCAGGACTGC
CTCGCCGCCGCCGAGCCGGCCGATCCGCTGCCGGTGGCCAGCGGCGATCCGCTGTACATCATGTACACCTCG
GGCACCACCGGTAAGCCCAAGGGCATCGTCCGCGACAACGGTGGCCACGCGGTGGCGGTGCGCTACGCGGTC
CGCACTATCTACGGCATGCAGGCCGGCGACGTCTGGTGGGGGATCTCCGACGTCGGCTGGGTGGTCGGCCAC
TCGCTGATCGTCTACGGCCCGCTGATGTGCGGCTGTACCACAGTGTTCTACGAAGGCAAGCCGGTGCGTACG
CCGGACGCCGGCGCCTACTGGCGGGTGATCGAAGAGCACAGGGTGAACAGCCTGTTCTGCGCGCCGACGGCG
ATCCGCGCGATCCGCAAGGAAGACCCGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCAC
CTGTTCCTGGCCGGCGAGAAGCTCGaCAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTC
CacgaCCATTGGTGGCaaaCCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGAC
CTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCG
CTGGGAGCGAACCGCCAGGGCACGATCGTCATCGCCCTGCCGTTGCCGCCGGGCTGCGCGCAGACCCTCTGG
GGCGACCACCCGCGCTACCTGCAGGCCTACCTGGGCAACTATCCCGGCTACTACCACACCGGCGACGGCGGT
TATCTCGACGAGGACGGCTTCGTCTACATCATGGGGCGCACCGACGACGTGATAAACGTTTCCGGGCATCGC
CTCTCCACCGGCGAGATGGAGGAACGCGTGGCGCAGCACCAGGCGGTTGCCGAGTGCGCGGTGATCGGCGTG
CGGGACGAACTCAAGGGCCACGTGCCGCTGGGCCTGGTGGTGCTCAAGGACGATGCCGGGATCGCCGCCGAG
CAACTGCAGCGCGAGCTGGTGGCGCTGGTCCGCGAACAGATCGGCGCGCTGGCCTGCTTCCAGCGGGTGGTG
GTGGTCAAGCGGCTGCCGAAGACCCGCTCCGGGAAGATCCTCCGCGCGGTGCTGCGCAAGATCGCCGATGGC
GAGGATTACGCGGCGCCATCGACCATCGACGACCCGACGATCCTTGGCGAGATCGAGGCTGCGCTGAGGGTG
CGCAAGGCGGGATAG
Translation:
MTYLQQHARSIAEPAAFWAEQARSLAWYQAPANILESLPDGTHRWFADGRLNSAYLALDRQIEEGRGEQTAL
IYDSPVTGTQDRYSYLRLRDEVARLAGALRALGVGKGDRVIIYMPMVPQAAMAMLACARLGAVHSVVFGGFA
PYELALRIDDATPKLVLTASCGLEFDRVIEYKPLVDKALELAIHQPAHVMVWQRPQALARLHPGRDLDWQDC
LAAAEPADPLPVASGDPLYIMYTSGTTGKPKGIVRDNGGHAVAVRYAVRTIYGMQAGDVWWGISDVGWVVGH
SLIVYGPLMCGCTTVFYEGKPVRTPDAGAYWRVIEEHRVNSLFCAPTAIRAIRKEDPHGERVKRYDLSSLRH
LFLAGEKLDSSTQHWLEEHTGRPVHDHWWQTETGWPVTAPCIGIAGHDLRAGSTNRAVPGYHVQVLDDEGRP
LGANRQGTIVIALPLPPGCAQTLWGDHPRYLQAYLGNYPGYYHTGDGGYLDEDGFVYIMGRTDDVINVSGHR
LSTGEMEERVAQHQAVAECAVIGVRDELKGHVPLGLVVLKDDAGIAAEQLQRELVALVREQIGALACFQRVV
VVKRLPKTRSGKILRAVLRKIADGEDYAAPSTIDDPTILGEIEAALRVRKAG*
AnnotationID:394GeneID:385
AnnotatorUID: diggins
Modification Date/Time: 2005-04-20 06:06:13
Gene Name:
Confidence Code:3
GeneProduct:putative acetyl-coa synthetase
Cell Localization:()
Synonyms:acsL
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(13) Fatty acid and phospholipid metabolism
Alternate Gene Product Name:putative Propionate--CoA ligase
Functional Category Confidence Code:5
COGs:COG0365,pfam00501,COG0318
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN67964.1|AE016428_12 (AE016782) acetyl-CoA synthetase, putative [Pseudomonas putida
           KT2440]
          Length = 629

 Score = 1003 bits (2593), Expect = 0.0
 Identities = 486/622 (78%), Positives = 539/622 (86%)

>gb|AAF94498.1| (AE004213) prpE protein [Vibrio cholerae]
          Length = 632

 Score =  789 bits (2038), Expect = 0.0
 Identities = 371/617 (60%), Positives = 473/617 (76%)

>emb|CAE35702.1| (BX640448) propionate--CoA ligase [Bordetella bronchiseptica]
          Length = 629

 Score =  527 bits (1358), Expect = e-149
 Identities = 279/627 (44%), Positives = 396/627 (63%), Gaps = 15/627 (2%)






 
Structural Features:
COG0365, Acs, Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases 
[Lipid metabolism]
CD-Length = 528 residues,  98.9% aligned
Score =  563 bits (1452), Expect = 2e-161
Identities = 215/558 (38%), Positives = 293/558 (52%), Gaps = 37/558 (6%)

pfam00501, AMP-binding, AMP-binding enzyme.
CD-Length = 412 residues, 100.0% aligned
Score =  290 bits (744), Expect = 3e-79
Identities = 141/441 (31%), Positives = 203/441 (46%), Gaps = 30/441 (6%)

COG0318, CaiC, Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II 
[Lipid metabolism / Secondary metabolites biosynthesis, transport, and catabolism]
CD-Length = 534 residues,  96.8% aligned
Score =  257 bits (657), Expect = 3e-69
Identities = 163/551 (29%), Positives = 239/551 (43%), Gaps = 37/551 (6%)






   
Genomic Context:






   
Comment:
other possible gene name: prpE


















    ReferenceID:773




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17915




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31973




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:385_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:385
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
643
                           PA3568_tr
                           translation of PA3568
                           628
                           99.52
                           99.52
                           0
                           3318.0
     

     
     
29581
                           gb|AAG06956.1|AE004777_9
                           (AE004777) probable acetyl-coa synthetase [Pseudomonas aeruginosa PAO1]
                           628
                           99.52
                           99.52
                           0
                           3318.0
     

     
     
29582
                           gb|AAN67964.1|AE016428_12
                           (AE016782) acetyl-CoA synthetase, putative [Pseudomonas putida KT2440]
                           629
                           77.58
                           86.32
                           1
                           2593.0
     

     
     
29583
                           gb|AAA25893.1|
                           (M84911) ORF located downstream of mmsAB operon, has sequence similarity to an acetyl-CoA synthetase; ORF1; putative [Pseudomonas aeruginosa]
                           628
                           73.40
                           73.40
                           164
                           2486.0
     

     
     
29586
                           dbj|BAC94297.1|
                           (AP005336) PrpE protein [Vibrio vulnificus YJ016]
                           629
                           58.98
                           74.08
                           5
                           2005.0
     

     






      

          
GC ORFID: 43387How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3568Glimmer Score: 

          
Start: 3396900Stop: 3395014

          
Length: 1887


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA3568 translation of PA3568Homolog Bit Score 1282.0

          
Other Homologs:  t_PA3568 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACGTACCTGCAGCAGCACGCCCGCTCGATCGCCGAGCCGGCCGCGTTCTGGGCCGAACAGGCCCGTTCC 
CTGGCCTGGTACCAGGCGCCCGCGAATATCCTCGAAAGCCTGCCCGACGGCACCCATCGCTGGTTCGCCGAT 
GGCCGCCTGAACAGCGCCTACCTGGCCCTCGACCGGCAGATCGAGGAGGGGCGCGGCGAACAGACCGCGCTG 
ATCTACGACTCGCCGGTGACCGGCACCCAGGACCGCTACAGCTACCTGCGGCTGCGCGACGAAGTGGCGCGC 
CTGGCCGGCGCCCTGCGGGCCCTCGGGGTCGGCAAGGGCGACCGGGTGATCATCTACATGCCGATGGTGCCG 
CAGGCGGCCATGGCGATGCTCGCTTGCGCGCGCCTGGGCGCGGTGCATTCGGTGGTGTTCGGTGGCTTCGCT 
CCCTACGAGCTGGCCCTGCGCATCGACGACGCCACGCCGAAGCTGGTGCTCACCGCTTCCTGCGGGCTGGAG 
TTCGACCGGGTGATCGAATACAAGCCGCTGGTGGACAAGGCCCTCGAACTGGCGATCCACCAGCCTGCCCAC 
GTCATGGTCTGGCAACGCCCGCAGGCGCTCGCGCGCCTGCACCCGGGCCGCGACCTGGACTGGCAGGACTGC 
CTCGCCGCCGCCGAGCCGGCCGATCCGCTGCCGGTGGCCAGCGGCGATCCGCTGTACATCATGTACACCTCG 
GGCACCACCGGTAAGCCCAAGGGCATCGTCCGCGACAACGGTGGCCACGCGGTGGCGGTGCGCTACGCGGTC 
CGCACTATCTACGGCATGCAGGCCGGCGACGTCTGGTGGGGGATCTCCGACGTCGGCTGGGTGGTCGGCCAC 
TCGCTGATCGTCTACGGCCCGCTGATGTGCGGCTGTACCACAGTGTTCTACGAAGGCAAGCCGGTGCGTACG 
CCGGACGCCGGCGCCTACTGGCGGGTGATCGAAGAGCACAGGGTGAACAGCCTGTTCTGCGCGCCGACGGCG 
ATCCGCGCGATCCGCAAGGAAGACCCGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCAC 
CTGTTCCTGGCCGGCGAGAAGCTCGaCAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTC 
CacgaCCATTGGTGGCaaaCCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGAC 
CTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCG 
CTGGGAGCGAACCGCCAGGGCACGATCGTCATCGCCCTGCCGTTGCCGCCGGGCTGCGCGCAGACCCTCTGG 
GGCGACCACCCGCGCTACCTGCAGGCCTACCTGGGCAACTATCCCGGCTACTACCACACCGGCGACGGCGGT 
TATCTCGACGAGGACGGCTTCGTCTACATCATGGGGCGCACCGACGACGTGATAAACGTTTCCGGGCATCGC 
CTCTCCACCGGCGAGATGGAGGAACGCGTGGCGCAGCACCAGGCGGTTGCCGAGTGCGCGGTGATCGGCGTG 
CGGGACGAACTCAAGGGCCACGTGCCGCTGGGCCTGGTGGTGCTCAAGGACGATGCCGGGATCGCCGCCGAG 
CAACTGCAGCGCGAGCTGGTGGCGCTGGTCCGCGAACAGATCGGCGCGCTGGCCTGCTTCCAGCGGGTGGTG 
GTGGTCAAGCGGCTGCCGAAGACCCGCTCCGGGAAGATCCTCCGCGCGGTGCTGCGCAAGATCGCCGATGGC 
GAGGATTACGCGGCGCCATCGACCATCGACGACCCGACGATCCTTGGCGAGATCGAGGCTGCGCTGAGGGTG 
CGCAAGGCGGGATAG