ViewGene
Locus Name:
GeneID:
Location:

GeneID:3061
TrivialName: PA14_62580
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_62580
PAO1 Ortholog Locus:PA4729
Sequence Length:801
Protein Length:266
Start: 5583394
Stop: 5584194
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08115.1|AE004886_7 (AE004886) 3-methyl-2-oxobutanoate hydroxymethyltransferase [Pseudomonas aeruginosa PAO1]
Identities = 265/266 (99%), Positives = 265/266 (99%)
Sequence:
ATGCCTGATGTAACCCTGACCACCCTGCAAGGGTTGAAGCAGAGCGGCGAGAAGATCGCCATGCTGACCTGC
TACGACGCCACCTTCGCCCACACCGCCAGCCAGGCCGGCGTGGACGTGCTTCTGGTCGGAGATTCGCTCGGA
ATGGTATTGCAGGGTCACGACAGCACCCTGCCGGTCAGCAACGAGGAAATGGCCTATCACACGGCCTGCGTG
AAACGCGGTAACAAAGGTTCCCTGATCGTCACCGACCTGGCCTTCGAATCCAGCCATTCCGTGGCCCAGACC
CTTGCCGACGCGGTCCGCCTGATGCAGGCCGGCGCACACATGGTCAAGCTGGAGGGTGGGGCCTGGCTGGCG
GAGCCGATCGCCCGCCTGGCACAGATGGGCGTGCCGGTCTGCGCGCATCTCGGCCTGACCCCGCAGGCGGTC
AACCTGTTCGGCGGCTTCAAGGTCCAGGGCCGCCAGGAAACCCAGGCGCGCCAGTTGCGCGCCGATGCCATC
GCCCTGGAGCAGGCCGGCGCCGCCATGCTGCTGCTGGAGTGCGTGCCCAGCGTGCTGGCCGAAGAGATCACC
CAGGCGGTGAAGATCCCGGTGATCGGGATTGGCGCCGGTGCGGCGACCGATGGCCAGGTGCTGGTCATGCAC
GACATGCTGGGTCTCTCGCTGACCGGCCGCTCGCCCAAGTTCGTCAAGGATTTCATGCAAGGCCAGGAGAGC
ATCCCAGCCGCCATCGCGGCCTACGTCCGCGCCGTCAAGGACGTGTCCTTCCCCGCCGCCGAACACGGATTC
AACGCATGA
Translation:
MPDVTLTTLQGLKQSGEKIAMLTCYDATFAHTASQAGVDVLLVGDSLGMVLQGHDSTLPVSNEEMAYHTACV
KRGNKGSLIVTDLAFESSHSVAQTLADAVRLMQAGAHMVKLEGGAWLAEPIARLAQMGVPVCAHLGLTPQAV
NLFGGFKVQGRQETQARQLRADAIALEQAGAAMLLLECVPSVLAEEITQAVKIPVIGIGAGAATDGQVLVMH
DMLGLSLTGRSPKFVKDFMQGQESIPAAIAAYVRAVKDVSFPAAEHGFNA*
AnnotationID:3069GeneID:3061
AnnotatorUID: diggins
Modification Date/Time: 2005-04-27 06:06:00
Gene Name:panB
Confidence Code:2
GeneProduct:3-methyl-2-oxobutanoate hydroxymethyltransferase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:ketopantoate hydroxymethyltransferase
Functional Category Confidence Code:5
COGs:COG0413,pfam02548
Secondary Functional Category(ies):central intermediary metabolism
EC Number:2.1.2.11
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70272.1|AE016667_5 (AE016791) 3-methyl-2-oxobutanoate hydroxymethyltransferase
           [Pseudomonas putida KT2440]
          Length = 266

 Score =  400 bits (1027), Expect = e-111
 Identities = 203/266 (76%), Positives = 226/266 (84%)

>gb|AAO54495.1| (AE016859) 3-methyl-2-oxobutanoate hydroxymethyltransferase
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 266

 Score =  397 bits (1019), Expect = e-110
 Identities = 195/266 (73%), Positives = 228/266 (85%)

>gb|AAN53946.1|AE015532_6 (AE015532) 3-methyl-2-oxobutanoate hydroxymethyltransferase
           [Shewanella oneidensis MR-1]
          Length = 264

 Score =  299 bits (765), Expect = 1e-80
 Identities = 150/265 (56%), Positives = 195/265 (73%), Gaps = 1/265 (0%)






 
Structural Features:
COG0413, PanB, Ketopantoate hydroxymethyltransferase 
[Coenzyme metabolism]
CD-Length = 268 residues,  98.5% aligned
Score =  350 bits (899), Expect = 1e-97
Identities = 153/265 (57%), Positives = 181/265 (68%), Gaps = 3/265 (1%)

pfam02548, Pantoate_transf, Ketopantoate hydroxymethyltransferase.
CD-Length = 261 residues,  99.2% aligned
Score =  300 bits (770), Expect = 1e-82
Identities = 140/260 (53%), Positives = 178/260 (68%), Gaps = 3/260 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:6125




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:23267




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37642




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3061_cdd.html


    		




 
URL:

















    ReferenceID:37738




  
Author/Investigator(s):
        Merkel WK, Nichols BP.





  
Title:
        Characterization and sequence of the Escherichia coli panBCD gene cluster.




  
PubMed:
        8837478


MedLine:
        




    
Source:
FEMS Microbiol Lett. 1996 Oct 1;143(2-3):247-52.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8837478

















    ReferenceID:37739




  
Author/Investigator(s):
        Jones CE, Brook JM, Buck D, Abell C, Smith AG.





  
Title:
        Cloning and sequencing of the Escherichia coli panB gene, which encodes ketopantoate hydroxymethyltransferase, and overexpression of the enzyme.




  
PubMed:
        8096212


MedLine:
        




    
Source:
J Bacteriol. 1993 Apr;175(7):2125-30.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8096212









           
Homologs By Global Alignment 

           

       



     
Gene ID:3061
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4940
                           PA4729_tr
                           translation of PA4729
                           266
                           99.62
                           99.62
                           0
                           1330.0
     

     
     
139615
                           gb|AAG08115.1|AE004886_7
                           (AE004886) 3-methyl-2-oxobutanoate hydroxymethyltransferase [Pseudomonas aeruginosa PAO1]
                           266
                           99.62
                           99.62
                           0
                           1330.0
     

     
     
139616
                           gb|AAN70272.1|AE016667_5
                           (AE016791) 3-methyl-2-oxobutanoate hydroxymethyltransferase [Pseudomonas putida KT2440]
                           266
                           76.31
                           84.96
                           0
                           1027.0
     

     
     
139619
                           gb|AAO60160.1|
                           (AY210414) 3-methyl-2-oxobutanoate hydroxymethyltransferase; PanB [Pseudomonas fluorescens]
                           266
                           73.30
                           83.83
                           0
                           1009.0
     

     
     
139618
                           emb|CAA10222.1|
                           (AJ130846) 3-methyl-2-oxobutanoate hydroxymethyltransferase [Pseudomonas fluorescens]
                           266
                           73.30
                           84.96
                           0
                           1011.0
     

     






      

          
GC ORFID: 46063How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4729Glimmer Score: 

          
Start: 4847074Stop: 4847874

          
Length: 801


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4729 translation of PA4729Homolog Bit Score 516.0

          
Other Homologs:  psyr_15may02_Scaffold15_revised_gene2520 (ORF: BLASTX 407.0), t_PA1598 (ORF: BLASTX 185.0), t_PA4729 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCCTGATGTAACCCTGACCACCCTGCAAGGGTTGAAGCAGAGCGGCGAGAAGATCGCCATGCTGACCTGC 
TACGACGCCACCTTCGCCCACACCGCCAGCCAGGCCGGCGTGGACGTGCTTCTGGTCGGAGATTCGCTCGGA 
ATGGTATTGCAGGGTCACGACAGCACCCTGCCGGTCAGCAACGAGGAAATGGCCTATCACACGGCCTGCGTG 
AAACGCGGTAACAAAGGTTCCCTGATCGTCACCGACCTGGCCTTCGAATCCAGCCATTCCGTGGCCCAGACC 
CTTGCCGACGCGGTCCGCCTGATGCAGGCCGGCGCACACATGGTCAAGCTGGAGGGTGGGGCCTGGCTGGCG 
GAGCCGATCGCCCGCCTGGCACAGATGGGCGTGCCGGTCTGCGCGCATCTCGGCCTGACCCCGCAGGCGGTC 
AACCTGTTCGGCGGCTTCAAGGTCCAGGGCCGCCAGGAAACCCAGGCGCGCCAGTTGCGCGCCGATGCCATC 
GCCCTGGAGCAGGCCGGCGCCGCCATGCTGCTGCTGGAGTGCGTGCCCAGCGTGCTGGCCGAAGAGATCACC 
CAGGCGGTGAAGATCCCGGTGATCGGGATTGGCGCCGGTGCGGCGACCGATGGCCAGGTGCTGGTCATGCAC 
GACATGCTGGGTCTCTCGCTGACCGGCCGCTCGCCCAAGTTCGTCAAGGATTTCATGCAAGGCCAGGAGAGC 
ATCCCAGCCGCCATCGCGGCCTACGTCCGCGCCGTCAAGGACGTGTCCTTCCCCGCCGCCGAACACGGATTC 
AACGCATGA