ViewGene
Locus Name:
GeneID:
Location:

GeneID:2910
TrivialName: PA14_19140
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_19140
PAO1 Ortholog Locus:PA3475
Sequence Length:807
Protein Length:268
Start: 1652656
Stop: 1653462
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06863.1|AE004768_8 (AE004768) cyclohexadienyl dehydratase precursor [Pseudomonas aeruginosa PAO1]
Identities = 267/268 (99%), Positives = 268/268 (100%)
Sequence:
ATGCCGAAGTCATTCCGCCATCTCGTCCAGGCCCTGGCCTGCCTTGCGCTGCTGGCCAGCGCCAGCCTCCAG
GCGCAGGAGAGCCGCCTCGACCGCATCCTCGAAAGCGGCGTGCTGCGCGTCGCCACCACGGGCGACTACAAG
CCCTTCAGCTACCGCACGGAAGAGGGCGGTTACGCCGGTTTCGACGTGGACATGGCGCAGCGCCTGGCCGAG
AGCCTGGGTGCCAAGCTGGTAGTGGTGCCGACCAGTTGGCCGAACCTGATGCGCGATTTCGCCGACGACCGC
TTCGACATCGCCATGAGCGGCATCTCGATCAACCTGGAGCGCCAGCGCCAGGCGTATTTCTCGATTCCCTAC
CTGCGCGACGGCAAGACGCCGATCACCCTCTGTAGCGAAGAAGCGCGTTTCCAGACCCTGGAGCAGATCGAC
CAGCCGGGCGTGACGGCCATCGTCAACCCCGGTGGCACCAACGAGAAGTTCGCCCGGGCGAACCTGAAGGAG
GCCCGGATCCTGGTGCATCCGGACAACGTGACGATCTTCCAGCAGATCGTCGACGGCAAGGCCGACCTGATG
ATGACCGACGCCATCGAGGCCCGCCTGCAGTCGCGGTTGCACCCGGAACTCTGCGCCGTGCATCCGCAGCAA
CCCTTCGACTTCGCCGAGAAGGCCTACCTGCTGCCGCGCGACGAGGCCTTCAAGCGCTACGTCGACCAGTGG
TTGCACATCGCTGAGCAGAGCGGCTTGTTGCGCCAGCGCATGGAGCACTGGCTCGAATACCGCTGGCCCACC
GCGCACGGCAAGTAG
Translation:
MPKSFRHLVQALACLALLASASLQAQESRLDRILESGVLRVATTGDYKPFSYRTEEGGYAGFDVDMAQRLAE
SLGAKLVVVPTSWPNLMRDFADDRFDIAMSGISINLERQRQAYFSIPYLRDGKTPITLCSEEARFQTLEQID
QPGVTAIVNPGGTNEKFARANLKEARILVHPDNVTIFQQIVDGKADLMMTDAIEARLQSRLHPELCAVHPQQ
PFDFAEKAYLLPRDEAFKRYVDQWLHIAEQSGLLRQRMEHWLEYRWPTAHGK*
AnnotationID:2918GeneID:2910
AnnotatorUID: diggins
Modification Date/Time: 2005-04-19 10:10:06
Gene Name:pheC
Confidence Code:1
GeneProduct:cyclohexadienyl dehydratase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:cd00134,smart00062,pfam00497,COG0834
Secondary Functional Category(ies):
EC Number:4.2.1.51
Status:ACTIVE
Pathway:






 
Homology:
>emb|CAD16219.1| (AL646070) PUTATIVE CYCLOHEXADIENYL DEHYDRATASE PRECURSOR SIGNAL
           PEPTIDE PROTEIN [Ralstonia solanacearum]
          Length = 267

 Score =  339 bits (869), Expect = 1e-92
 Identities = 162/253 (64%), Positives = 198/253 (78%), Gaps = 1/253 (0%)

>gb|AAM42807.1| (AE012474) cyclohexadienyl dehydratase [Xanthomonas campestris pv.
           campestris str. ATCC 33913]
          Length = 256

 Score =  243 bits (621), Expect = 6e-64
 Identities = 121/244 (49%), Positives = 169/244 (69%), Gaps = 1/244 (0%)

>gb|AAO54857.1| (AE016860) cyclohexadienyl dehydratase, putative [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 263

 Score =  236 bits (603), Expect = 8e-62
 Identities = 121/256 (47%), Positives = 170/256 (66%), Gaps = 2/256 (0%)






 
Structural Features:
cd00134, PBPb, periplasmic binding proteins (PBPs)
CD-Length = 218 residues, 100.0% aligned
Score =  158 bits (400), Expect = 8e-40
Identities = 58/222 (26%), Positives = 95/222 (42%), Gaps = 6/222 (2%)

smart00062, PBPb, Bacterial periplasmic substrate-binding proteins
CD-Length = 222 residues,  98.6% aligned
Score =  162 bits (412), Expect = 3e-41
Identities = 63/224 (28%), Positives = 107/224 (47%), Gaps = 8/224 (3%)

pfam00497, SBP_bac_3, Bacterial extracellular solute-binding proteins, family 3.
CD-Length = 224 residues,  99.1% aligned
Score =  124 bits (314), Expect = 8e-30
Identities = 60/225 (26%), Positives = 104/225 (46%), Gaps = 8/225 (3%)

COG0834, HisJ, ABC-type amino acid transport
CD-Length = 275 residues,  97.8% aligned
Score =  127 bits (319), Expect = 1e-30
Identities = 71/273 (26%), Positives = 118/273 (43%), Gaps = 14/273 (5%)






   
Genomic Context:






   
Comment:


















    ReferenceID:5823




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22965




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32084




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2910_cdd.html


    		




 
URL:

















    ReferenceID:34076




  
Author/Investigator(s):
        Zhao G, Xia T, Aldrich H, Jensen RA.





  
Title:
        Cyclohexadienyl dehydratase from Pseudomonas aeruginosa is a periplasmic protein.




  
PubMed:
        8515238


MedLine:
        




    
Source:
J Gen Microbiol. 1993 Apr;139 ( Pt 4):807-13.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8515238

















    ReferenceID:34077




  
Author/Investigator(s):
        Zhao GS, Xia TH, Fischer RS, Jensen RA.





  
Title:
        Cyclohexadienyl dehydratase from Pseudomonas aeruginosa. Molecular cloning of the gene and characterization of the gene product.




  
PubMed:
        1733946


MedLine:
        




    
Source:
J Biol Chem. 1992 Feb 5;267(4):2487-93.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1733946









           
Homologs By Global Alignment 

           

       



     
Gene ID:2910
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4711
                           PA3475_tr
                           translation of PA3475
                           268
                           99.62
                           99.99
                           0
                           1388.0
     

     
     
135511
                           gb|AAG06863.1|AE004768_8
                           (AE004768) cyclohexadienyl dehydratase precursor [Pseudomonas aeruginosa PAO1]
                           268
                           99.62
                           99.99
                           0
                           1388.0
     

     
     
135512
                           gb|AAC08596.1|
                           (AF054868) cyclohexadienyl dehydratase [Pseudomonas aeruginosa]
                           268
                           98.13
                           99.25
                           0
                           1368.0
     

     
     
135513
                           emb|CAD16219.1|
                           (AL646070) PUTATIVE CYCLOHEXADIENYL DEHYDRATASE PRECURSOR SIGNAL PEPTIDE PROTEIN [Ralstonia solanacearum]
                           271
                           60.88
                           75.27
                           7
                           865.0
     

     
     
135514
                           gb|AAQ57903.1|
                           (AE016910) probable carboxycyclohexadienyl dehydratase [Chromobacterium violaceum ATCC 12472]
                           269
                           55.76
                           65.05
                           14
                           731.0
     

     






      

          
GC ORFID: 45912How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3475Glimmer Score: 

          
Start: 3301277Stop: 3300471

          
Length: 807


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA3475 translation of PA3475Homolog Bit Score 539.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene4925 (ORF: BLASTX 244.0), t_PA3475 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCCGAAGTCATTCCGCCATCTCGTCCAGGCCCTGGCCTGCCTTGCGCTGCTGGCCAGCGCCAGCCTCCAG 
GCGCAGGAGAGCCGCCTCGACCGCATCCTCGAAAGCGGCGTGCTGCGCGTCGCCACCACGGGCGACTACAAG 
CCCTTCAGCTACCGCACGGAAGAGGGCGGTTACGCCGGTTTCGACGTGGACATGGCGCAGCGCCTGGCCGAG 
AGCCTGGGTGCCAAGCTGGTAGTGGTGCCGACCAGTTGGCCGAACCTGATGCGCGATTTCGCCGACGACCGC 
TTCGACATCGCCATGAGCGGCATCTCGATCAACCTGGAGCGCCAGCGCCAGGCGTATTTCTCGATTCCCTAC 
CTGCGCGACGGCAAGACGCCGATCACCCTCTGTAGCGAAGAAGCGCGTTTCCAGACCCTGGAGCAGATCGAC 
CAGCCGGGCGTGACGGCCATCGTCAACCCCGGTGGCACCAACGAGAAGTTCGCCCGGGCGAACCTGAAGGAG 
GCCCGGATCCTGGTGCATCCGGACAACGTGACGATCTTCCAGCAGATCGTCGACGGCAAGGCCGACCTGATG 
ATGACCGACGCCATCGAGGCCCGCCTGCAGTCGCGGTTGCACCCGGAACTCTGCGCCGTGCATCCGCAGCAA 
CCCTTCGACTTCGCCGAGAAGGCCTACCTGCTGCCGCGCGACGAGGCCTTCAAGCGCTACGTCGACCAGTGG 
TTGCACATCGCTGAGCAGAGCGGCTTGTTGCGCCAGCGCATGGAGCACTGGCTCGAATACCGCTGGCCCACC 
GCGCACGGCAAGTAG