ViewGene
Locus Name:
GeneID:
Location:

GeneID:2882
TrivialName: PA14_06540
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_06540
PAO1 Ortholog Locus:PA0503
Sequence Length:825
Protein Length:274
Start: 572694
Stop: 573518
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03892.1|AE004487_6 (AE004487) probable biotin synthesis protein BioC [Pseudomonas aeruginosa PAO1]
Identities = 270/274 (98%), Positives = 274/274 (100%)
Sequence:
ATGCCTGACGATTCCGCGCCGCTGCTGGCGCCCCATGGCGTTGCCGCGCTGCCCGACAAGCGCCGGGTCGCC
GCGTCGTTTTCCCGCGCGGCGGCCAGCTACGACGCGGTGGCCGAACTCCAGCGCGGCGTCGGCGAGAGCCTG
CTGTCGGCATTGCCGGAAGGCTTCTCGCCGCGCCGCTGGGTCGATCTCGGCTGCGGCACCGGTCATTTCAGC
CGGGCCCTGGAACGCCGCTTTGGCGCGGCCGAGGGCCTGGCTGTGGACATCGCCGAAGGCATGCTGCGGCAT
GCCCGCGCGCGCGGCGGCGCCAGCCATTTCATCGGCGGCGACGCCGAGAGGCTGCCGTTGCGCGACGGCAGT
TGCGACCTGCTGTTCTCCAGCCTGGCCATCCAGTGGTGCGCCGACCTCCCGGCGGTCCTGGCCGAGGCGCGG
CGGGTCCTGCGGCCGGGCGGCGTGCTGGCGTTCAGCAGCCTGTGCGTCGGTACCCTGGGCGAGTTGCGCGAC
AGTTGGCGGGTGGTGGACGGCTTCGTCCACGTCAATCGCTTCCGCGCCTTCGCCGACTACCTGCAACACGCG
GCCGGCAGCGGTCTGCTGCCGCTGACCCTGCGCCACGAGGACCGGGTCCTGCATTTCCCCGACCTGCGCAGC
CTGACCCACGAACTCAAGGCGCTGGGCGCGCACAACCTCAACCCCGGGCGGCCCGACGGCCTGACCGGGCGC
CAGCGCATCCGCGCGCTGGTCGCCGCCTACGAGCGTTTCCGCCAGCCCGAGGGGCTGCCCGCTACCTACCGC
GTCGTCTTCGGCGTGCTGCGCAAGGATTCCTGA
Translation:
MPDDSAPLLAPHGVAALPDKRRVAASFSRAAASYDAVAELQRGVGESLLSALPEGFSPRRWVDLGCGTGHFS
RALERRFGAAEGLAVDIAEGMLRHARARGGASHFIGGDAERLPLRDGSCDLLFSSLAIQWCADLPAVLAEAR
RVLRPGGVLAFSSLCVGTLGELRDSWRVVDGFVHVNRFRAFADYLQHAAGSGLLPLTLRHEDRVLHFPDLRS
LTHELKALGAHNLNPGRPDGLTGRQRIRALVAAYERFRQPEGLPATYRVVFGVLRKDS*
AnnotationID:2890GeneID:2882
AnnotatorUID: diggins
Modification Date/Time: 2004-08-15 09:09:37
Gene Name:
Confidence Code:3
GeneProduct:putative biotin synthesis protein BioC
Cell Localization:()
Synonyms:bioC
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO54041.1| (AE016857) biotin synthesis protein BioC [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 269

 Score =  372 bits (954), Expect = e-102
 Identities = 185/262 (70%), Positives = 210/262 (80%)

>gb|AAN65996.1|AE016228_9 (AE016775) biotin biosynthesis protein BioC [Pseudomonas putida
           KT2440]
          Length = 272

 Score =  362 bits (928), Expect = 2e-99
 Identities = 189/273 (69%), Positives = 209/273 (76%), Gaps = 5/273 (1%)






 
Structural Features:






   
Genomic Context:






   
Comment:


















    ReferenceID:5767




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22910




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33814




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2882_cdd.html


    		




 
URL:

















    ReferenceID:35319




  
Author/Investigator(s):
        Kiyasu T, Nagahashi Y, Hoshino T.





  
Title:
        Cloning and characterization of biotin biosynthetic genes of Kurthia sp.




  
PubMed:
        11255013


MedLine:
        




    
Source:
Gene. 2001 Mar 7;265(1-2):103-13.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11255013

















    ReferenceID:35320




  
Author/Investigator(s):
        Wu CH, Bao YY, Shao CP, Shiuan D.





  
Title:
        Molecular cloning and nucleotide sequencing of bioF (7-keto-8-amino pelargonic acid synthetase), bioC and bioD (dethiobiotin synthetase) genes of Erwinia herbicola.




  
PubMed:
        9063571


MedLine:
        




    
Source:
Biochem Mol Biol Int. 1997 Feb;41(2):311-5.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9063571

















    ReferenceID:35321




  
Author/Investigator(s):
        Sakurai N, Akatsuka H, Kawai E, Imai Y, Komatsubara S.





  
Title:
        Complete sequence and organization of the Serratia marcescens biotin operon.




  
PubMed:
        8969526


MedLine:
        




    
Source:
Microbiology. 1996 Nov;142 ( Pt 11):3295-303.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8969526

















    ReferenceID:35326




  
Author/Investigator(s):
        Otsuka AJ, Buoncristiani MR, Howard PK, Flamm J, Johnson C, Yamamoto R, Uchida K, Cook C, Ruppert J, Matsuzaki J.





  
Title:
        The Escherichia coli biotin biosynthetic enzyme sequences predicted from the nucleotide sequence of the bio operon.




  
PubMed:
        3058702


MedLine:
        




    
Source:
J Biol Chem. 1988 Dec 25;263(36):19577-85.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3058702









           
Homologs By Global Alignment 

           

       



     
Gene ID:2882
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4676
                           PA0503_tr
                           translation of PA0503
                           274
                           98.54
                           99.99
                           0
                           1401.0
     

     
     
134734
                           gb|AAG03892.1|AE004487_6
                           (AE004487) probable biotin synthesis protein BioC [Pseudomonas aeruginosa PAO1]
                           274
                           98.54
                           99.99
                           0
                           1401.0
     

     
     
134735
                           gb|AAO54041.1|
                           (AE016857) biotin synthesis protein BioC [Pseudomonas syringae pv. tomato str. DC3000]
                           274
                           69.34
                           78.10
                           5
                           951.0
     

     
     
134736
                           gb|AAN65996.1|AE016228_9
                           (AE016775) biotin biosynthesis protein BioC [Pseudomonas putida KT2440]
                           276
                           68.47
                           75.72
                           6
                           930.0
     

     
     
134737
                           dbj|BAA04287.1|
                           (D17468) the product of bioC [Serratia marcescens]
                           289
                           41.86
                           55.36
                           49
                           514.0
     

     






      

          
GC ORFID: 45884How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0503Glimmer Score: 

          
Start: 6374036Stop: 6374860

          
Length: 825


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0503 translation of PA0503Homolog Bit Score 544.0

          
Other Homologs:  t_PA0503 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCCTGACGATTCCGCGCCGCTGCTGGCGCCCCATGGCGTTGCCGCGCTGCCCGACAAGCGCCGGGTCGCC 
GCGTCGTTTTCCCGCGCGGCGGCCAGCTACGACGCGGTGGCCGAACTCCAGCGCGGCGTCGGCGAGAGCCTG 
CTGTCGGCATTGCCGGAAGGCTTCTCGCCGCGCCGCTGGGTCGATCTCGGCTGCGGCACCGGTCATTTCAGC 
CGGGCCCTGGAACGCCGCTTTGGCGCGGCCGAGGGCCTGGCTGTGGACATCGCCGAAGGCATGCTGCGGCAT 
GCCCGCGCGCGCGGCGGCGCCAGCCATTTCATCGGCGGCGACGCCGAGAGGCTGCCGTTGCGCGACGGCAGT 
TGCGACCTGCTGTTCTCCAGCCTGGCCATCCAGTGGTGCGCCGACCTCCCGGCGGTCCTGGCCGAGGCGCGG 
CGGGTCCTGCGGCCGGGCGGCGTGCTGGCGTTCAGCAGCCTGTGCGTCGGTACCCTGGGCGAGTTGCGCGAC 
AGTTGGCGGGTGGTGGACGGCTTCGTCCACGTCAATCGCTTCCGCGCCTTCGCCGACTACCTGCAACACGCG 
GCCGGCAGCGGTCTGCTGCCGCTGACCCTGCGCCACGAGGACCGGGTCCTGCATTTCCCCGACCTGCGCAGC 
CTGACCCACGAACTCAAGGCGCTGGGCGCGCACAACCTCAACCCCGGGCGGCCCGACGGCCTGACCGGGCGC 
CAGCGCATCCGCGCGCTGGTCGCCGCCTACGAGCGTTTCCGCCAGCCCGAGGGGCTGCCCGCTACCTACCGC 
GTCGTCTTCGGCGTGCTGCGCAAGGATTCCTGA