ViewGene
Locus Name:
GeneID:
Location:

GeneID:2706
TrivialName: PA14_25190
AnnotatorUID: danlee
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_25190
PAO1 Ortholog Locus:
Sequence Length:996
Protein Length:331
Start: 2202589
Stop: 2203583
Strand: +
Type:
ChromosomeID:2
Status: INACTIVE
Frame Discrepancy: TRUE
Missense Discrepancy:TRUE
Comments:
Inactivated 2/01/06. PA14_25190 and PA14_25200 replaced by PA14_25195 (GeneID 7376). Homology: gb|AAG06393.1|AE004725_6 (AE004725) beta-N-acetyl-D-glucosaminidase [Pseudomonas aeruginosa PAO1]
Identities = 296/329 (89%), Positives = 301/329 (91%), Gaps = 2/329 (0%)
Sequence:
ATGCAAGGCTCTCTGATGCTCGACATCGGCGGCACCTGGCTGACCGCCGAGGACCGCCAAATCCTGCGCCAC
CCCGAGGTGGGCGGGCTGATCATCTTCGCTCGCAACATCGAGCATCCTGCCCAGGTTCGCGAACTGTGTGCG
GCCATTCGCGCCATCCGTCCCGACCTGTTGCTGGCCGTCGACCAGGAAGGAGGGCGCGTGCAGCGCCTGCGC
CAGGGCTTCGTGCGCCTGCCGGCGATGCGCGCGATCGCCGACAACCCCAATGCCGAAGAGCTGGCCGAGCAC
TGTGGCTGGTTGATGGCGACCGAGGTGCAGGCGGTAGGCCTCGACCTGAGTTTCGCGCCGGTGCTGGATCTC
GATCACCAGCGCAGCGCCGTGGTCGGTAGCCGCGCCTTCGAGGGCGACCCGGAGCGTGCCGCGCTGCTTGCC
GGTGCTTTCATTCGCGGCATGCATGCCGCCGGGATGGCCGCCACCGGCAAGCATTTTCCCGGTCACGGCTGG
GCCGAGGCCGACTCCCATGTGGCGATTCCCGAAGACGCGCGCAGCCTCGAGGAAATCCGGCGCAGCGACCTG
GTTCCGTTCGCCCGCCTGGCCGGACAGCTCGACGCGCTGATGCCGGCCCATGTCATCTATCCGCAGGTCGAC
CCGCAGCCCGCCGGCTTTTCCCGGCGCTGGTTGCAGGAGATCCTGCGCGGCGAGCTGAAGTTCGACGGGGTG
ATCTTCAGCGACGACCTGTCGATGGCCGGCGCCCATGTGGTCGGCGACGCCGCCAGCCGCATCGAGGCCGCC
CTGGCCGCGGGCTGCGACATGGGTCTGGTGTGCAACGACCGGGCTTCCGCCGAGCTGGCCCTGGCTGccCCT
GCAACGCCTGAAGGTGACCCCGCCGTCGCGTCTGCAACGCATGCGCGGCAAGGGCTACGCGAATACCGACTA
TCGCCAGCAGCCGCGCTGGCTGGAAGCACTGTCCGCGTTGCGCGCCGCGCAACTGATTGA
Translation:
MQGSLMLDIGGTWLTAEDRQILRHPEVGGLIIFARNIEHPAQVRELCAAIRAIRPDLLLAVDQEGGRVQRLR
QGFVRLPAMRAIADNPNAEELAEHCGWLMATEVQAVGLDLSFAPVLDLDHQRSAVVGSRAFEGDPERAALLA
GAFIRGMHAAGMAATGKHFPGHGWAEADSHVAIPEDARSLEEIRRSDLVPFARLAGQLDALMPAHVIYPQVD
PQPAGFSRRWLQEILRGELKFDGVIFSDDLSMAGAHVVGDAASRIEAALAAGCDMGLVCNDRASAELALAAP
ATPEGDPAVASATHARQGLREYRLSPAAALAGSTVRVARRATD*
AnnotationID:2714GeneID:2706
AnnotatorUID: danlee
Modification Date/Time: 2006-02-01 13:01:17
Gene Name:
Confidence Code:4
GeneProduct:
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:()
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam00933
Secondary Functional Category(ies):
EC Number:3.2.1.52
Status:INACTIVE
Pathway:






 
Homology:
>gb|AAM52310.1|AF502251_3 (AF502251) beta-N-acetylglucosaminidase-like protein [Pseudomonas
           chlororaphis]
          Length = 332

 Score =  517 bits (1331), Expect = e-146
 Identities = 263/316 (83%), Positives = 282/316 (89%), Gaps = 2/316 (0%)

>gb|AAO56982.1| (AE016868) beta-hexosaminidase [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 336

 Score =  505 bits (1300), Expect = e-142
 Identities = 260/329 (79%), Positives = 280/329 (85%), Gaps = 2/329 (0%)

>emb|CAC90437.1| (AJ414149) beta-hexosaminidase [Yersinia pestis CO92]
          Length = 343

 Score =  301 bits (771), Expect = 4e-81
 Identities = 161/285 (56%), Positives = 199/285 (69%), Gaps = 7/285 (2%)








 
Structural Features:
pfam00933, Glyco_hydro_3, Glycosyl hydrolase family 3 N terminal domain.
CD-Length = 226 residues,  95.6% aligned
Score =  162 bits (412), Expect = 4e-41
Identities = 71/221 (32%), Positives = 100/221 (45%), Gaps = 8/221 (3%)






   
Genomic Context:






   
Comment:


















    ReferenceID:5415




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22560




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36043




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2706_cdd.html


    		




 
URL:

















    ReferenceID:36475




  
Author/Investigator(s):
        Cheng Q, Li H, Merdek K, Park JT.





  
Title:
        Molecular characterization of the beta-N-acetylglucosaminidase of Escherichia coli and its role in cell wall recycling.




  
PubMed:
        10940025


MedLine:
        




    
Source:
J Bacteriol. 2000 Sep;182(17):4836-40.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10940025









           
Homologs By Global Alignment 

           

       



     
Gene ID:2706
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4432
                           PA3005_tr
                           translation of PA3005
                           339
                           87.90
                           89.08
                           15
                           1471.0
     

     
     
128787
                           gb|AAG06393.1|AE004725_6
                           (AE004725) beta-N-acetyl-D-glucosaminidase [Pseudomonas aeruginosa PAO1]
                           339
                           87.90
                           89.08
                           15
                           1471.0
     

     
     
128788
                           gb|AAM52310.1|AF502251_3
                           (AF502251) beta-N-acetylglucosaminidase-like protein [Pseudomonas chlororaphis]
                           333
                           79.57
                           86.18
                           3
                           1328.0
     

     
     
128789
                           gb|AAO56982.1|
                           (AE016868) beta-hexosaminidase [Pseudomonas syringae pv. tomato str. DC3000]
                           337
                           77.15
                           83.08
                           7
                           1298.0
     

     
     
128790
                           gb|AAN67758.1|AE016407_2
                           (AE016782) beta-hexosaminidase [Pseudomonas putida KT2440]
                           337
                           76.55
                           83.67
                           7
                           1287.0
     

     






      

          
GC ORFID: 45708How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3005Glimmer Score: 

          
Start: 2751338Stop: 2750343

          
Length: 996


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3005 translation of PA3005Homolog Bit Score 571.0

          
Other Homologs:  psyr_15may02_Scaffold9_revised_gene8052 (ORF: BLASTX 503.0), t_PA3005 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCAAGGCTCTCTGATGCTCGACATCGGCGGCACCTGGCTGACCGCCGAGGACCGCCAAATCCTGCGCCAC 
CCCGAGGTGGGCGGGCTGATCATCTTCGCTCGCAACATCGAGCATCCTGCCCAGGTTCGCGAACTGTGTGCG 
GCCATTCGCGCCATCCGTCCCGACCTGTTGCTGGCCGTCGACCAGGAAGGAGGGCGCGTGCAGCGCCTGCGC 
CAGGGCTTCGTGCGCCTGCCGGCGATGCGCGCGATCGCCGACAACCCCAATGCCGAAGAGCTGGCCGAGCAC 
TGTGGCTGGTTGATGGCGACCGAGGTGCAGGCGGTAGGCCTCGACCTGAGTTTCGCGCCGGTGCTGGATCTC 
GATCACCAGCGCAGCGCCGTGGTCGGTAGCCGCGCCTTCGAGGGCGACCCGGAGCGTGCCGCGCTGCTTGCC 
GGTGCTTTCATTCGCGGCATGCATGCCGCCGGGATGGCCGCCACCGGCAAGCATTTTCCCGGTCACGGCTGG 
GCCGAGGCCGACTCCCATGTGGCGATTCCCGAAGACGCGCGCAGCCTCGAGGAAATCCGGCGCAGCGACCTG 
GTTCCGTTCGCCCGCCTGGCCGGACAGCTCGACGCGCTGATGCCGGCCCATGTCATCTATCCGCAGGTCGAC 
CCGCAGCCCGCCGGCTTTTCCCGGCGCTGGTTGCAGGAGATCCTGCGCGGCGAGCTGAAGTTCGACGGGGTG 
ATCTTCAGCGACGACCTGTCGATGGCCGGCGCCCATGTGGTCGGCGACGCCGCCAGCCGCATCGAGGCCGCC 
CTGGCCGCGGGCTGCGACATGGGTCTGGTGTGCAACGACCGGGCTTCCGCCGAGCTGGCCCTGGCTGccCCT 
GCAACGCCTGAAGGTGACCCCGCCGTCGCGTCTGCAACGCATGCGCGGCAAGGGCTACGCGAATACCGACTA 
TCGCCAGCAGCCGCGCTGGCTGGAAGCACTGTCCGCGTTGCGCGCCGCGCAACTGATTGA