ViewGene
Locus Name:
GeneID:
Location:

GeneID:2627
TrivialName: PA14_18140
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_18140
PAO1 Ortholog Locus:PA3569
Sequence Length:897
Protein Length:298
Start: 1556038
Stop: 1556934
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06957.1|AE004778_1 (AE004778) 3-hydroxyisobutyrate dehydrogenase [Pseudomonas aeruginosa PAO1]
Identities = 294/298 (98%), Positives = 296/298 (99%)
Sequence:
ATGACCGACATCGCATTCCTCGGCCTGGGCAACATGGGTGGGCCGATGGCCGCCAACCTGCTCAAGGCTGGC
CACCGGGTGAATGTCTTCGACTTGCAGCCCAAGGCCGTGCTGAGCCTGGTCGAGCAGGGCGCGCAGGGCGCC
GATAGCGCCTTGCAGTGCTGCGAAGGCGCCGAAGTGGTGATCAGCATGCTGCCGGCCGGGCAGCACGTGGAA
AGCCTGTATCTCGGCGCCGACGGCCTGCTCGCGCGGGTCGCCGGCAAGCCCCTACTGATCGACTGCTCGACC
ATTGCCCCGGAGACCGCGCGCAAGGTCGCCGAGGCCGCCGCGGCGAAGGGCCTGACCCTGCTCGACGCGCCG
GTTTCCGGCGGCGTCGGCGGCGCCCGCGCCGGCACCCTGAGCTTCATCGTCGGCGGCCCCGCCGAAGGCTTC
GCGCGGGCCCGGCCGATCCTCGAGAACATGGGCCGGAACATCTTCCACGCCGGCGATCACGGCGCCGGCCAG
GTGGCGAAGATCTGCAACAACATGCTCCTCGGCATCCTCATGGCCGGCACCGCCGAGGCCCTGGCGCTGGGG
GTGAAGAACGGCCTCGATCCGGCGGTGCTGTCCGAGGTGATGAAGCAGAGTTCCGGCGGCAACTGGGCGCTG
AACCTCTACAACCCCTGGCCCGGGGTGATGCCGCAGGCGCCGGCGAGCAACGGCTATGCCGGCGGTTTCCAG
GTGCGCCTGATGAACAAGGACCTCGGCCTGGCGCTGGCCAACGCCCAGGCGGTGCAGGCCTCGACGCCACTC
GGCGCGCTGGCGCGCAACCTGTTCAGCTTGCACGCCCAGGCCGATGCCGAGCACGACGGGCTGGACTTCTCC
AGCATCCAGAAGCTCTACCGCGGCAAGGACTGA
Translation:
MTDIAFLGLGNMGGPMAANLLKAGHRVNVFDLQPKAVLSLVEQGAQGADSALQCCEGAEVVISMLPAGQHVE
SLYLGADGLLARVAGKPLLIDCSTIAPETARKVAEAAAAKGLTLLDAPVSGGVGGARAGTLSFIVGGPAEGF
ARARPILENMGRNIFHAGDHGAGQVAKICNNMLLGILMAGTAEALALGVKNGLDPAVLSEVMKQSSGGNWAL
NLYNPWPGVMPQAPASNGYAGGFQVRLMNKDLGLALANAQAVQASTPLGALARNLFSLHAQADAEHDGLDFS
SIQKLYRGKD*
AnnotationID:2635GeneID:2627
AnnotatorUID: diggins
Modification Date/Time: 2005-04-20 06:06:21
Gene Name:mmsB
Confidence Code:1
GeneProduct: 3-hydroxyisobutyrate dehydrogenase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(5) Carbon compound catabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG2084,KOG0409
Secondary Functional Category(ies):
EC Number:1.1.1.31
Status:ACTIVE
Pathway:






 
Homology:
>emb|CAE33125.1| (BX640445) 3-hydroxyisobutyrate dehydrogenase [Bordetella
           bronchiseptica]
          Length = 297

 Score =  380 bits (977), Expect = e-105
 Identities = 196/294 (66%), Positives = 230/294 (78%), Gaps = 3/294 (1%)

>gb|AAM36187.1| (AE011763) 3-hydroxyisobutirate dehydrogenase [Xanthomonas
           axonopodis pv. citri str. 306]
          Length = 295

 Score =  356 bits (914), Expect = 8e-98
 Identities = 181/294 (61%), Positives = 222/294 (75%), Gaps = 2/294 (0%)

>dbj|BAC61968.1| (AP005086) putative 3-hydroxyisobutyrate dehydrogenase [Vibrio
           parahaemolyticus]
          Length = 300

 Score =  314 bits (805), Expect = 3e-85
 Identities = 168/299 (56%), Positives = 207/299 (69%), Gaps = 6/299 (2%)









 
Structural Features:
COG2084, MmsB, 3-hydroxyisobutyrate dehydrogenase and related 
beta-hydroxyacid dehydrogenases 
[Lipid metabolism]
CD-Length = 286 residues, 100.0% aligned
Score =  280 bits (717), Expect = 4e-76
Identities = 148/295 (50%), Positives = 184/295 (62%), Gaps = 10/295 (3%)

KOG0409, Predicted dehydrogenase
CD-Length = 327 residues,  87.8% aligned
Score =  248 bits (635), Expect = 1e-66
Identities = 124/296 (41%), Positives = 174/296 (58%), Gaps = 10/296 (3%)







   
Genomic Context:






   
Comment:


















    ReferenceID:5257




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22402




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31971




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2627_cdd.html


    		




 
URL:

















    ReferenceID:34154




  
Author/Investigator(s):
        Steele MI, Lorenz D, Hatter K, Park A, Sokatch JR.





  
Title:
        Characterization of the mmsAB operon of Pseudomonas aeruginosa PAO encoding methylmalonate-semialdehyde dehydrogenase and 3-hydroxyisobutyrate dehydrogenase.




  
PubMed:
        1339433


MedLine:
        




    
Source:
J Biol Chem. 1992 Jul 5;267(19):13585-92.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1339433

















    ReferenceID:38102




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2627_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:2627
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4342
                           PA3569_tr
                           translation of PA3569
                           298
                           98.65
                           99.32
                           0
                           1501.0
     

     
     
127137
                           gb|AAG06957.1|AE004778_1
                           (AE004778) 3-hydroxyisobutyrate dehydrogenase [Pseudomonas aeruginosa PAO1]
                           298
                           98.65
                           99.32
                           0
                           1501.0
     

     
     
127138
                           gb|AAA25892.1|
                           (M84911) 3-hydroxyisobutyrate dehydrogenase [Pseudomonas aeruginosa]
                           298
                           98.65
                           99.32
                           0
                           1501.0
     

     
     
127140
                           emb|CAE41737.1|
                           (BX640415) 3-hydroxyisobutyrate dehydrogenase [Bordetella pertussis] [Bordetella pertussis Tohama I]
                           300
                           65.33
                           77.00
                           5
                           978.0
     

     
     
127139
                           emb|CAE33125.1|
                           (BX640445) 3-hydroxyisobutyrate dehydrogenase [Bordetella bronchiseptica] [Bordetella bronchiseptica RB50]
                           300
                           65.33
                           77.00
                           5
                           978.0
     

     






      

          
GC ORFID: 45629How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3569Glimmer Score: 

          
Start: 3397898Stop: 3397002

          
Length: 897


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3569 translation of PA3569Homolog Bit Score 582.0

          
Other Homologs:  t_PA0743 (ORF: BLASTX 352.0), psyr_15may02_Scaffold2_revised_gene3515 (ORF: BLASTX 296.0), t_PA3569 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACCGACATCGCATTCCTCGGCCTGGGCAACATGGGTGGGCCGATGGCCGCCAACCTGCTCAAGGCTGGC 
CACCGGGTGAATGTCTTCGACTTGCAGCCCAAGGCCGTGCTGAGCCTGGTCGAGCAGGGCGCGCAGGGCGCC 
GATAGCGCCTTGCAGTGCTGCGAAGGCGCCGAAGTGGTGATCAGCATGCTGCCGGCCGGGCAGCACGTGGAA 
AGCCTGTATCTCGGCGCCGACGGCCTGCTCGCGCGGGTCGCCGGCAAGCCCCTACTGATCGACTGCTCGACC 
ATTGCCCCGGAGACCGCGCGCAAGGTCGCCGAGGCCGCCGCGGCGAAGGGCCTGACCCTGCTCGACGCGCCG 
GTTTCCGGCGGCGTCGGCGGCGCCCGCGCCGGCACCCTGAGCTTCATCGTCGGCGGCCCCGCCGAAGGCTTC 
GCGCGGGCCCGGCCGATCCTCGAGAACATGGGCCGGAACATCTTCCACGCCGGCGATCACGGCGCCGGCCAG 
GTGGCGAAGATCTGCAACAACATGCTCCTCGGCATCCTCATGGCCGGCACCGCCGAGGCCCTGGCGCTGGGG 
GTGAAGAACGGCCTCGATCCGGCGGTGCTGTCCGAGGTGATGAAGCAGAGTTCCGGCGGCAACTGGGCGCTG 
AACCTCTACAACCCCTGGCCCGGGGTGATGCCGCAGGCGCCGGCGAGCAACGGCTATGCCGGCGGTTTCCAG 
GTGCGCCTGATGAACAAGGACCTCGGCCTGGCGCTGGCCAACGCCCAGGCGGTGCAGGCCTCGACGCCACTC 
GGCGCGCTGGCGCGCAACCTGTTCAGCTTGCACGCCCAGGCCGATGCCGAGCACGACGGGCTGGACTTCTCC 
AGCATCCAGAAGCTCTACCGCGGCAAGGACTGA