ViewGene
Locus Name:
GeneID:
Location:

GeneID:2491
TrivialName: PA14_42700
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_42700
PAO1 Ortholog Locus:PA1686
Sequence Length:894
Protein Length:297
Start: 3798594
Stop: 3799487
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05075.1|AE004596_1 (AE004596) DNA-3-methyladenine glycosidase II [Pseudomonas aeruginosa PAO1]
Identities = 296/297 (99%), Positives = 297/297 (100%)
Sequence:
ATGAGCCAGCCTACCTCTCCCCTCGTCCTGCACCTGCCCTACCAGTCACCCTGGGAATGGCGCCAGTTCCAC
CAGCATTTCGCCCTGCGCCTGCTCGCCGGTGTCGAAAGCCTGGGCGACGACCACTATGCCCGCAGCTTCCGG
GCCAACGGCCGCCCGGCCTGGTTCGAGGTGCGACCGCTGGCGGAACGCCAGGTCCTGGCGCTGAGCCTCAGC
CCGTCCGCCCATGCCCTGGCCGCCGAACTCGAGGCACGGGTCCGCCGGATGTTCGACCTGGACAGCGACCCG
GCCGCCATCGCCCGGCATTTCGCCGGGGATCCCCTGCTCGGCCCGCTGGTGGCCGCCAATCCGGGGCTGCGC
CTGCCGGTGGCCTTCGACCCCTTCGAACAGGCGGTGCGCGCCATCGTCGGCCAGCAGGTCACGGTGAAGGCC
GCGGTGACCATCACCGGGCGCCTGATCCAGCGCCTCGGCGAGCCGCTGGAAAATCTCGGCTACGACGGCATC
AGCCACCTGTTCCCGACACCCGCCGCGCTGGCGCAAGCCAACCTCGACGGCATCGGCATGCCCGGCAAGCGG
GTACAGACGCTGCAACGCTTCGCCGCGGCGATCGCCTCGGGAGAGCTGAGCCTGGACCTGGCGGACGGCCCC
GAGGCGCTGGTGGAGCGCCTCTGCGCGTTGCCCGGCATCGGCCCCTGGACCGCCGAATACATAGCCCTGCGT
GCCATGGGCGAAGCCGACGCCTTCCCCGCCGCCGACCTCGGCCTGCTGAAATCCACGGTCTGGGGGCCGCAA
GGCATCGATGCGCGCAGCCTCAAGGCCCGCGCCGAGGCCTGGCGGCCTTGGCGCGCCTACGCCGCCATCCAC
CTGTGGCACCACTACGCCGCAGGCGGCTAG
Translation:
MSQPTSPLVLHLPYQSPWEWRQFHQHFALRLLAGVESLGDDHYARSFRANGRPAWFEVRPLAERQVLALSLS
PSAHALAAELEARVRRMFDLDSDPAAIARHFAGDPLLGPLVAANPGLRLPVAFDPFEQAVRAIVGQQVTVKA
AVTITGRLIQRLGEPLENLGYDGISHLFPTPAALAQANLDGIGMPGKRVQTLQRFAAAIASGELSLDLADGP
EALVERLCALPGIGPWTAEYIALRAMGEADAFPAADLGLLKSTVWGPQGIDARSLKARAEAWRPWRAYAAIH
LWHHYAAGG*
AnnotationID:2499GeneID:2491
AnnotatorUID: diggins
Modification Date/Time: 2005-04-28 07:07:35
Gene Name:alkA
Confidence Code:2
GeneProduct:DNA-3-methyladenine glycosidase II
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(11) DNA replication, recombination, modification and repair
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0122,pfam06029
Secondary Functional Category(ies):
EC Number:3.2.2.21
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAM41924.1| (AE012377) DNA methylation and regulatory protein Ada or
           [Xanthomonas campestris pv. campestris str. ATCC 33913]
          Length = 487

 Score =  211 bits (537), Expect = 4e-54
 Identities = 116/288 (40%), Positives = 159/288 (55%), Gaps = 2/288 (0%)






 
Structural Features:
COG0122, AlkA, 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase 
[DNA replication, recombination, and repair]
CD-Length = 285 residues,  97.2% aligned
Score =  219 bits (560), Expect = 2e-58
Identities = 116/288 (40%), Positives = 150/288 (52%), Gaps = 19/288 (6%)

pfam06029, AlkA_N, AlkA N-terminal domain.
CD-Length = 116 residues, 100.0% aligned
Score =  135 bits (341), Expect = 6e-33
Identities = 57/118 (48%), Positives = 65/118 (55%), Gaps = 2/118 (1%)







   
Genomic Context:






   
Comment:


















    ReferenceID:4985




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22131




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37254




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2491_cdd.html


    		




 
URL:

















    ReferenceID:37871




  
Author/Investigator(s):
        Nakabeppu Y, Miyata T, Kondo H, Iwanaga S, Sekiguchi M.





  
Title:
        Structure and expression of the alkA gene of Escherichia coli involved in adaptive response to alkylating agents.




  
PubMed:
        6094528


MedLine:
        




    
Source:
 J Biol Chem. 1984 Nov 25;259(22):13730-6.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6094528

















    ReferenceID:37872




  
Author/Investigator(s):
        Morohoshi F, Hayashi K, Munkata N.





  
Title:
        Bacillus subtilis alkA gene encoding inducible 3-methyladenine DNA glycosylase is adjacent to the ada operon.




  
PubMed:
        8376346


MedLine:
        




    
Source:
J Bacteriol. 1993 Sep;175(18):6010-7.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8376346









           
Homologs By Global Alignment 

           

       



     
Gene ID:2491
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4172
                           PA1686_tr
                           translation of PA1686
                           297
                           99.66
                           99.99
                           0
                           1544.0
     

     
     
123607
                           gb|AAG05075.1|AE004596_1
                           (AE004596) DNA-3-methyladenine glycosidase II [Pseudomonas aeruginosa PAO1]
                           297
                           99.66
                           99.99
                           0
                           1544.0
     

     
     
123611
                           gb|AAB06751.1|
                           (U65786) 3-methyladenine glycosidase [Mycobacterium tuberculosis]
                           309
                           29.44
                           40.77
                           87
                           386.0
     

     
     
123612
                           dbj|BAA15921.1|
                           (D90844) DNA-3-methyladenine glycosidase II (EC 3.2.2.21) (3-methyladenine-DNA glycosylase I, inducible) (TAG II). [Escherichia coli]
                           306
                           27.12
                           35.29
                           115
                           326.0
     

     
     
123608
                           dbj|BAC91550.1|
                           (AP006580) AraC family transcriptional regulatory protein [Gloeobacter violaceus PCC 7421]
                           535
                           26.16
                           33.45
                           238
                           611.0
     

     






      

          
GC ORFID: 45493How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1686Glimmer Score: 

          
Start: 1155315Stop: 1154422

          
Length: 894


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA1686 translation of PA1686Homolog Bit Score 599.0

          
Other Homologs:  t_PA1686 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCCAGCCTACCTCTCCCCTCGTCCTGCACCTGCCCTACCAGTCACCCTGGGAATGGCGCCAGTTCCAC 
CAGCATTTCGCCCTGCGCCTGCTCGCCGGTGTCGAAAGCCTGGGCGACGACCACTATGCCCGCAGCTTCCGG 
GCCAACGGCCGCCCGGCCTGGTTCGAGGTGCGACCGCTGGCGGAACGCCAGGTCCTGGCGCTGAGCCTCAGC 
CCGTCCGCCCATGCCCTGGCCGCCGAACTCGAGGCACGGGTCCGCCGGATGTTCGACCTGGACAGCGACCCG 
GCCGCCATCGCCCGGCATTTCGCCGGGGATCCCCTGCTCGGCCCGCTGGTGGCCGCCAATCCGGGGCTGCGC 
CTGCCGGTGGCCTTCGACCCCTTCGAACAGGCGGTGCGCGCCATCGTCGGCCAGCAGGTCACGGTGAAGGCC 
GCGGTGACCATCACCGGGCGCCTGATCCAGCGCCTCGGCGAGCCGCTGGAAAATCTCGGCTACGACGGCATC 
AGCCACCTGTTCCCGACACCCGCCGCGCTGGCGCAAGCCAACCTCGACGGCATCGGCATGCCCGGCAAGCGG 
GTACAGACGCTGCAACGCTTCGCCGCGGCGATCGCCTCGGGAGAGCTGAGCCTGGACCTGGCGGACGGCCCC 
GAGGCGCTGGTGGAGCGCCTCTGCGCGTTGCCCGGCATCGGCCCCTGGACCGCCGAATACATAGCCCTGCGT 
GCCATGGGCGAAGCCGACGCCTTCCCCGCCGCCGACCTCGGCCTGCTGAAATCCACGGTCTGGGGGCCGCAA 
GGCATCGATGCGCGCAGCCTCAAGGCCCGCGCCGAGGCCTGGCGGCCTTGGCGCGCCTACGCCGCCATCCAC 
CTGTGGCACCACTACGCCGCAGGCGGCTAG