ViewGene
Locus Name:
GeneID:
Location:

GeneID:2454
TrivialName: PA14_10660
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_10660
PAO1 Ortholog Locus:PA4120
Sequence Length:912
Protein Length:303
Start: 918509
Stop: 919420
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07507.1|AE004828_8 (AE004828) probable transcriptional regulator [Pseudomonas aeruginosa PAO1]
Identities = 301/303 (99%), Positives = 301/303 (99%)
Sequence:
ATGCCAGAGCGCCAGCCGATCCCCAACATCAACATTGGGCAGGTCTACGACCAGCGCTATGCCGACGCCGAG
GTGCACTACGATGCGCTGGGCAACCTGGCCGGATTCTTCGGCCGTAACATGCCGGCGCACCGGCACGACCGT
TTCTTCCAGGTGCACTACGTGAAGAACGGCGCGGTGCGGGTCTATCTCGACGAGCGCCAGTACCTGGAGTCG
GGGCCGATGTTCTTCCTCACCCCGCCGACCGTGCCGCACGCCTTCGTCACCGAGGCGGACGCCGATGGCCAT
GTGCTGACCGTGCGCCAGCAACTGGTGTGGAGCCTCACCGAGGCCGAGCCGGGCCTGGCGCCCGGCCCGCAG
GTGGCGCCGGCCTGCGTGGCGCTGGGCAACCTGGAGGGCGGCGCGGCCGAGGAGGCGGCGCGCCTGGCGCTG
CTCTTCGAGCAGTTGCGCAGCGAGTCGATGGCCAGCCGTCCCGGCCGCCAGCAGACGCTGCTGGCGCTGACC
CGCCTGGTCATGATCAGCCTGCTGCGTCTCTCGGCCAATTCGGTGGCTGCGCGGCCGATGCGCCACGAAGAC
CTGCAGATCTTCCAGCGCTTCAACGCGCTGATCGAGGAGCGCTATGCCGAGCACTGGCCGCTGTCGCTCTAC
GCCAGCCGCATCGGCGTCACCGAGGCGCGCCTCAACGACGTCTGCCGGCGCATCGCCGACCTGCCGTCGAAG
CGCCTGGTGTACGAACGGCTTATGCAGGAGTCCAAGCGCCTGCTGCTGTTCACCGGCGGCTCGGTGAACGAG
ATCTGCTACCAGCTCGGCTTCAAGGACCCGGCCTACTTCAGTCGTTTCTTCGTGCGTTACGCGGGGCTTACC
CCCAGCGCTTACCGCCAGCGACAGGCCGACGGGGAGTCCCGTCGCTGA
Translation:
MPERQPIPNINIGQVYDQRYADAEVHYDALGNLAGFFGRNMPAHRHDRFFQVHYVKNGAVRVYLDERQYLES
GPMFFLTPPTVPHAFVTEADADGHVLTVRQQLVWSLTEAEPGLAPGPQVAPACVALGNLEGGAAEEAARLAL
LFEQLRSESMASRPGRQQTLLALTRLVMISLLRLSANSVAARPMRHEDLQIFQRFNALIEERYAEHWPLSLY
ASRIGVTEARLNDVCRRIADLPSKRLVYERLMQESKRLLLFTGGSVNEICYQLGFKDPAYFSRFFVRYAGLT
PSAYRQRQADGESRR*
AnnotationID:2462GeneID:2454
AnnotatorUID: diggins
Modification Date/Time: 2005-06-07 09:09:57
Gene Name:
Confidence Code:3
GeneProduct:putative transcriptional regulator
Cell Localization:()
Synonyms:hpaA
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(25) Transcriptional regulators
Alternate Gene Product Name:putative 4-hydroxyphenylacetate 3-monooxygenase operon regulatory protein
Functional Category Confidence Code:5
COGs:smart00342
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAL20040.1| (AE008748) 4-hydroxyphenylacetate catabolism protein [Salmonella
           typhimurium LT2]
          Length = 298

 Score =  263 bits (673), Expect = 7e-70
 Identities = 134/295 (45%), Positives = 193/295 (65%), Gaps = 7/295 (2%)

>gb|AAD53494.1|AF144422_1 (AF144422) HpaA [Salmonella enterica subsp. enterica serovar
           Dublin]
          Length = 298

 Score =  257 bits (657), Expect = 5e-68
 Identities = 132/295 (44%), Positives = 193/295 (65%), Gaps = 7/295 (2%)

>emb|CAA86047.1| (Z37980) regulator of the 4HPA-hydroxylase operon [Escherichia
           coli]
          Length = 295

 Score =  255 bits (652), Expect = 2e-67
 Identities = 133/298 (44%), Positives = 194/298 (65%), Gaps = 9/298 (3%)






 
Structural Features:
smart00342, HTH_ARAC, helix_turn_helix, arabinose operon control protein
CD-Length = 84 residues,  98.8% aligned
Score = 69.1 bits (169), Expect = 6e-13
Identities = 28/83 (33%), Positives = 45/83 (54%)






   
Genomic Context:






   
Comment:


















    ReferenceID:4911




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22057




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36853




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2454_cdd.html


    		




 
URL:

















    ReferenceID:36955




  
Author/Investigator(s):
        Prieto MA, Garcia JL.





  
Title:
        Identification of a novel positive regulator of the 4-hydroxyphenylacetate catabolic pathway of Escherichia coli.




  
PubMed:
        9126350


MedLine:
        




    
Source:
Biochem Biophys Res Commun. 1997 Mar 27;232(3):759-65.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9126350

















    ReferenceID:36956




  
Author/Investigator(s):
        Prieto MA, Diaz E, Garcia JL.





  
Title:
        Molecular characterization of the 4-hydroxyphenylacetate catabolic pathway of Escherichia coli W: engineering a mobile aromatic degradative cluster.




  
PubMed:
        8550403


MedLine:
        




    
Source:
J Bacteriol. 1996 Jan;178(1):111-20.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8550403









           
Homologs By Global Alignment 

           

       



     
Gene ID:2454
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4119
                           PA4120_tr
                           translation of PA4120
                           303
                           99.33
                           99.33
                           0
                           1555.0
     

     
     
122953
                           gb|AAG07507.1|AE004828_8
                           (AE004828) probable transcriptional regulator [Pseudomonas aeruginosa PAO1]
                           303
                           99.33
                           99.33
                           0
                           1555.0
     

     
     
122954
                           gb|AAL20040.1|
                           (AE008748) 4-hydroxyphenylacetate catabolism protein [Salmonella typhimurium LT2]
                           308
                           45.12
                           63.96
                           15
                           676.0
     

     
     
122955
                           gb|AAO69433.1|
                           (AE016840) 4-hydroxyphenylacetate 3-monooxygenase operon regulatory protein [Salmonella enterica subsp. enterica serovar Typhi Ty2]
                           308
                           45.12
                           63.96
                           15
                           676.0
     

     
     
122956
                           emb|CAD08234.1|
                           (AL627269) 4-hydroxyphenylacetate 3-monooxygenase operon regulatory protein [Salmonella enterica subsp. enterica serovar Typhi]
                           308
                           45.12
                           63.96
                           15
                           676.0
     

     






      

          
GC ORFID: 45456How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4120Glimmer Score: 

          
Start: 4035428Stop: 4034517

          
Length: 912


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4120 translation of PA4120Homolog Bit Score 603.0

          
Other Homologs:  t_PA4120 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCCAGAGCGCCAGCCGATCCCCAACATCAACATTGGGCAGGTCTACGACCAGCGCTATGCCGACGCCGAG 
GTGCACTACGATGCGCTGGGCAACCTGGCCGGATTCTTCGGCCGTAACATGCCGGCGCACCGGCACGACCGT 
TTCTTCCAGGTGCACTACGTGAAGAACGGCGCGGTGCGGGTCTATCTCGACGAGCGCCAGTACCTGGAGTCG 
GGGCCGATGTTCTTCCTCACCCCGCCGACCGTGCCGCACGCCTTCGTCACCGAGGCGGACGCCGATGGCCAT 
GTGCTGACCGTGCGCCAGCAACTGGTGTGGAGCCTCACCGAGGCCGAGCCGGGCCTGGCGCCCGGCCCGCAG 
GTGGCGCCGGCCTGCGTGGCGCTGGGCAACCTGGAGGGCGGCGCGGCCGAGGAGGCGGCGCGCCTGGCGCTG 
CTCTTCGAGCAGTTGCGCAGCGAGTCGATGGCCAGCCGTCCCGGCCGCCAGCAGACGCTGCTGGCGCTGACC 
CGCCTGGTCATGATCAGCCTGCTGCGTCTCTCGGCCAATTCGGTGGCTGCGCGGCCGATGCGCCACGAAGAC 
CTGCAGATCTTCCAGCGCTTCAACGCGCTGATCGAGGAGCGCTATGCCGAGCACTGGCCGCTGTCGCTCTAC 
GCCAGCCGCATCGGCGTCACCGAGGCGCGCCTCAACGACGTCTGCCGGCGCATCGCCGACCTGCCGTCGAAG 
CGCCTGGTGTACGAACGGCTTATGCAGGAGTCCAAGCGCCTGCTGCTGTTCACCGGCGGCTCGGTGAACGAG 
ATCTGCTACCAGCTCGGCTTCAAGGACCCGGCCTACTTCAGTCGTTTCTTCGTGCGTTACGCGGGGCTTACC 
CCCAGCGCTTACCGCCAGCGACAGGCCGACGGGGAGTCCCGTCGCTGA