ViewGene
Locus Name:
GeneID:
Location:

GeneID:2421
TrivialName: PA14_43320
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_43320
PAO1 Ortholog Locus:PA1638
Sequence Length:909
Protein Length:302
Start: 3859392
Stop: 3858484
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05027.1|AE004591_7 (AE004591) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
Identities = 301/302 (99%), Positives = 301/302 (99%)
Sequence:
ATGCAGCAACTGTTGAACGAGATTCTCGATGAAGTCCGCCCGCTGATCGGGCGCGGCAAGGTCGCCGACTAC
ATTCCCGCGCTCGCCGGCGTCGAGCCGAACCAACTGGGGATCGCCGTGTACAGCCGCGACGGCGAGCTGTTC
CATGCCGGCGACGCCCTGCGGCCGTTTTCCATCCAGAGCATTTCCAAGGTGTTCAGCCTGGTCCAGGCGATC
CAGCATTCCGGCGAGGACATCTGGCAACGCCTCGGCCACGAGCCCTCCGGCCAGCCTTTCAACTCCCTGGTG
CAGCTGGAGTTCGAGCGCGGCAAGCCGCGCAATCCGTTCATCAATGCCGGCGCCCTGGTGATCTGCGACATC
AACCAGTCGCGTTTCGCCGCGCCGGCGCAATCGATGCGCGATTTCGTCCGGCGCCTGTGCGGCAACCCGGAA
GTGGTGTCGGACTCGGTGGTGGCGCGCTCGGAGTACCAGCACCGCTCGCGCAACGCGGCGGCGGCCTACCTG
ATGAAATCGTTCGGCAACTTCCACAACGACGTCGAGGCGGTGCTGCTCAGCTATTTCCATCATTGCGCGTTG
CGCATGAGCTGCGTCGACCTGGCGCGGGCGTTCTGCTTCCTCGCCGACAAGGGCTTCTGCAAGCACAGCGGC
GAGCAGGTGCTCAACGAGCGGCAGACCAAGCAGGTCAACGCGATCATGGCCACCAGCGGGCTCTACGACGAG
GCCGGCAACTTTGCCTACCGGGTCGGCCTGCCGGGCAAGAGCGGCGTCGGCGGCGGCATCATCGCGGTGGTG
CCGGGGCGCTTCACGGTCTGCGTCTGGTCGCCGGAGCTGAACGCCGCGGGCAATTCGCTGGCGGGTATCGCC
GCGCTGGAAAAGCTCAGCGAGCGGATCGGCTGGTCGATCTTCTGA
Translation:
MQQLLNEILDEVRPLIGRGKVADYIPALAGVEPNQLGIAVYSRDGELFHAGDALRPFSIQSISKVFSLVQAI
QHSGEDIWQRLGHEPSGQPFNSLVQLEFERGKPRNPFINAGALVICDINQSRFAAPAQSMRDFVRRLCGNPE
VVSDSVVARSEYQHRSRNAAAAYLMKSFGNFHNDVEAVLLSYFHHCALRMSCVDLARAFCFLADKGFCKHSG
EQVLNERQTKQVNAIMATSGLYDEAGNFAYRVGLPGKSGVGGGIIAVVPGRFTVCVWSPELNAAGNSLAGIA
ALEKLSERIGWSIF*
AnnotationID:2429GeneID:2421
AnnotatorUID: diggins
Modification Date/Time: 2005-05-31 07:07:17
Gene Name:
Confidence Code:3
GeneProduct:putative glutaminase
Cell Localization:()
Synonyms:glsA
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam04960
Secondary Functional Category(ies):central intermediary metabolism
EC Number:3.5.1.2
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO55995.1| (AE016864) glutaminase, putative [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 302

 Score =  499 bits (1284), Expect = e-140
 Identities = 244/302 (80%), Positives = 267/302 (88%)

>gb|AAM86886.1|AE013935_3 (AE013935) putative glutaminase [Yersinia pestis KIM]
          Length = 308

 Score =  383 bits (984), Expect = e-106
 Identities = 192/300 (64%), Positives = 238/300 (79%), Gaps = 1/300 (0%)

>gb|AAN80404.1|AE016760_263 (AE016760) Probable glutaminase yneH [Escherichia coli CFT073]
          Length = 308

 Score =  380 bits (975), Expect = e-105
 Identities = 189/300 (63%), Positives = 239/300 (79%), Gaps = 1/300 (0%)








 
Structural Features:
pfam04960, Glutaminase
CD-Length = 286 residues, 100.0% aligned
Score =  427 bits (1100), Expect = 6e-121
Identities = 160/286 (55%), Positives = 204/286 (71%), Gaps = 2/286 (0%)






   
Genomic Context:






   
Comment:
other possible gene name: yneH


















    ReferenceID:4845




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21991




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37222




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2421_cdd.html


    		




 
URL:

















    ReferenceID:38180




  
Author/Investigator(s):
        Calderon J, Huerta-Saquero A, Du Pont G, Duran S.





  
Title:
        Sequence and molecular analysis of the Rhizobium etli glsA gene, encoding a thermolabile glutaminase.




  
PubMed:
        10095071


MedLine:
        




    
Source:
Biochim Biophys Acta. 1999 Mar 19;1444(3):451-6.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10095071&query_hl=4









           
Homologs By Global Alignment 

           

       



     
Gene ID:2421
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4071
                           PA1638_tr
                           translation of PA1638
                           302
                           99.66
                           99.66
                           0
                           1563.0
     

     
     
122110
                           gb|AAG05027.1|AE004591_7
                           (AE004591) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
                           302
                           99.66
                           99.66
                           0
                           1563.0
     

     
     
122111
                           gb|AAO55995.1|
                           (AE016864) glutaminase, putative [Pseudomonas syringae pv. tomato str. DC3000]
                           302
                           80.79
                           88.41
                           0
                           1284.0
     

     
     
122121
                           dbj|BAC60886.1|
                           (AP005082) glutaminase family protein [Vibrio parahaemolyticus]
                           306
                           63.39
                           76.79
                           4
                           974.0
     

     
     
122126
                           gb|AAO09943.1|AE016801_262
                           (AE016801) Glutaminase [Vibrio vulnificus CMCP6]
                           306
                           62.41
                           76.47
                           4
                           966.0
     

     






      

          
GC ORFID: 45423How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1638Glimmer Score: 

          
Start: 1094517Stop: 1095425

          
Length: 909


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1638 translation of PA1638Homolog Bit Score 606.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene1259 (ORF: BLASTX 501.0), t_PA1638 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCAGCAACTGTTGAACGAGATTCTCGATGAAGTCCGCCCGCTGATCGGGCGCGGCAAGGTCGCCGACTAC 
ATTCCCGCGCTCGCCGGCGTCGAGCCGAACCAACTGGGGATCGCCGTGTACAGCCGCGACGGCGAGCTGTTC 
CATGCCGGCGACGCCCTGCGGCCGTTTTCCATCCAGAGCATTTCCAAGGTGTTCAGCCTGGTCCAGGCGATC 
CAGCATTCCGGCGAGGACATCTGGCAACGCCTCGGCCACGAGCCCTCCGGCCAGCCTTTCAACTCCCTGGTG 
CAGCTGGAGTTCGAGCGCGGCAAGCCGCGCAATCCGTTCATCAATGCCGGCGCCCTGGTGATCTGCGACATC 
AACCAGTCGCGTTTCGCCGCGCCGGCGCAATCGATGCGCGATTTCGTCCGGCGCCTGTGCGGCAACCCGGAA 
GTGGTGTCGGACTCGGTGGTGGCGCGCTCGGAGTACCAGCACCGCTCGCGCAACGCGGCGGCGGCCTACCTG 
ATGAAATCGTTCGGCAACTTCCACAACGACGTCGAGGCGGTGCTGCTCAGCTATTTCCATCATTGCGCGTTG 
CGCATGAGCTGCGTCGACCTGGCGCGGGCGTTCTGCTTCCTCGCCGACAAGGGCTTCTGCAAGCACAGCGGC 
GAGCAGGTGCTCAACGAGCGGCAGACCAAGCAGGTCAACGCGATCATGGCCACCAGCGGGCTCTACGACGAG 
GCCGGCAACTTTGCCTACCGGGTCGGCCTGCCGGGCAAGAGCGGCGTCGGCGGCGGCATCATCGCGGTGGTG 
CCGGGGCGCTTCACGGTCTGCGTCTGGTCGCCGGAGCTGAACGCCGCGGGCAATTCGCTGGCGGGTATCGCC 
GCGCTGGAAAAGCTCAGCGAGCGGATCGGCTGGTCGATCTTCTGA