ViewGene
Locus Name:
GeneID:
Location:

GeneID:2236
TrivialName: PA14_58130
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_58130
PAO1 Ortholog Locus:PA4480
Sequence Length:993
Protein Length:330
Start: 5175905
Stop: 5174913
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07868.1|AE004862_2 (AE004862) rod shape-determining protein MreC [Pseudomonas aeruginosa PAO1]
Identities = 330/330 (100%), Positives = 330/330 (100%)
Sequence:
GTGCTGGCCGTGCTGTCGGTCAGCCTGATGGTGGTGGACGCCCGGTTCGACTATCTGGAGCCCGTCCGCAGC
AAGCTGGGGATGGTGCTCACGCCTTTCTACGGCCTGGCGGAAATGCCGGTGCGCGCCTGGGAAGGCGTGCGC
GACCAGTTCAGCAGCCGTAGCGAGCTGATCGCCGAGAACGAACGCCTCAAGGCCGAGTCCCTGCTGATGCAG
CGCCGGGTGCAGAAACTCGCCGCGCTCACCGAGCAGAACGTGCGCCTGCGCGAGCTGCTCAATTCCGCCGCG
CTGGTCGACGACAAGGTACTGGTCAGCGAGCTGATCGGCGTCGATCCGAACCCCTTCACCCAACGCATCATG
ATCGACAAGGGCGAGAACGACGGCGTCTTCGTCGGCCAGCCGGTGCTCGATGCCAGCGGCCTGATGGGCCAG
GTGGTCGAGGTGATGCCCTATACCGCGCGGGTCCTGCTGCTCACCGACACCACCCACAGTATCCCGGTGCAG
GTCAATCGCAACGGCCTGCGCGCCATCGCCGTCGGCACCGGCAATCCCGAGCGCTTGGAATTGCGCTACGTC
GCCGACACCGCCGACATCAAGGAAGGCGACCTACTGGTCAGCTCCGGCCTGGGGCAACGCTTCCCCGCCGGC
TATCCGGTGGCCACGGTCAAGGAAGTGATCCACGATTCCGGCCAGCCGTTCGCCGTGGTGCGCGCGGTGCCG
ACCGCGAAGATGAACCGTAGCCGCTACGTGCTGCTGGTGTTCAGCGACAGCCGCACCCCGGAACAGCGCGCC
AACGACGCCGCCGAGGCCCAGGAAGAGGCGGACAAGAAAGCCGCCGCCGGCGCCCAGGCGCCCCAGCCTGCC
GCGCAACCGGCCGCCGCGCCGTCGCCGGCCACCCCGGCAGCCCAGGGTGCTGCGCAGCAGCCCGCCGCCGCG
CCCGCGCCAGCTCCCACGCAGCCTGCCGCTCCGGCGGCGAACGGGGGACGCCGCTGA
Translation:
MLAVLSVSLMVVDARFDYLEPVRSKLGMVLTPFYGLAEMPVRAWEGVRDQFSSRSELIAENERLKAESLLMQ
RRVQKLAALTEQNVRLRELLNSAALVDDKVLVSELIGVDPNPFTQRIMIDKGENDGVFVGQPVLDASGLMGQ
VVEVMPYTARVLLLTDTTHSIPVQVNRNGLRAIAVGTGNPERLELRYVADTADIKEGDLLVSSGLGQRFPAG
YPVATVKEVIHDSGQPFAVVRAVPTAKMNRSRYVLLVFSDSRTPEQRANDAAEAQEEADKKAAAGAQAPQPA
AQPAAAPSPATPAAQGAAQQPAAAPAPAPTQPAAPAANGGRR*
AnnotationID:2244GeneID:2236
AnnotatorUID: diggins
Modification Date/Time: 2005-05-03 14:02:34
Gene Name:mreC
Confidence Code:2
GeneProduct:rod shape-determining protein MreC
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(7) Cell wall / LPS / capsule
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1792,pfam04085
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66559.1|AE016284_7 (AE016777) rod shape determining protein MreC [Pseudomonas putida
           KT2440]
          Length = 332

 Score =  468 bits (1205), Expect = e-131
 Identities = 247/332 (74%), Positives = 280/332 (84%), Gaps = 17/332 (5%)

>gb|AAF22468.1|AF063934_2 (AF063934) rod shape determining protein MreC [Pseudomonas
           fluorescens]
          Length = 366

 Score =  451 bits (1159), Expect = e-126
 Identities = 233/324 (71%), Positives = 269/324 (83%)

>gb|AAC76282.1| (AE000404) rod shape-determining protein [Escherichia coli K12]
          Length = 367

 Score =  223 bits (568), Expect = 1e-57
 Identities = 132/333 (39%), Positives = 195/333 (58%), Gaps = 16/333 (4%)






 
Structural Features:
COG1792, MreC, Cell shape-determining protein 
[Cell envelope biogenesis, outer membrane]
CD-Length = 284 residues,  93.7% aligned
Score =  258 bits (660), Expect = 2e-69
Identities = 104/266 (39%), Positives = 150/266 (56%), Gaps = 1/266 (0%)

pfam04085, MreC, rod shape-determining protein MreC.
CD-Length = 153 residues, 100.0% aligned
Score =  192 bits (489), Expect = 1e-49
Identities = 74/153 (48%), Positives = 98/153 (64%)






   
Genomic Context:






   
Comment:


















    ReferenceID:14545




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21621




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31519




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2236_cdd.html


    		




 
URL:

















    ReferenceID:32873




  
Author/Investigator(s):
        Wachi M, Doi M, Ueda T, Ueki M, Tsuritani K, Nagai K, Matsuhashi M.





  
Title:
        Sequence of the downstream flanking region of the shape-determining genes mreBCD of Escherichia coli.




  
PubMed:
        1937035


MedLine:
        




    
Source:
Gene. 1991 Sep 30;106(1):135-6.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1937035

















    ReferenceID:32874




  
Author/Investigator(s):
        Wachi M, Doi M, Okada Y, Matsuhashi M.





  
Title:
        New mre genes mreC and mreD, responsible for formation of the rod shape of Escherichia coli cells.




  
PubMed:
        2687239


MedLine:
        




    
Source:
J Bacteriol. 1989 Dec;171(12):6511-6.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2687239

















    ReferenceID:32875




  
Author/Investigator(s):
        Kubo M, Pierro DJ, Mochizuki Y, Kojima T, Yamazaki T, Satoh S, Takizawa N, Kiyohara H.





  
Title:
        Bacillus stearothermophilus cell shape determinant gene, mreC and mreD, and their stimulation of protease production in Bacillus subtilis.




  
PubMed:
        9063975


MedLine:
        




    
Source:
Biosci Biotechnol Biochem. 1996 Feb;60(2):271-6.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9063975









           
Homologs By Global Alignment 

           

       



     
Gene ID:2236
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3794
                           PA4480_tr
                           translation of PA4480
                           330
                           99.99
                           99.99
                           0
                           1640.0
     

     
     
116960
                           gb|AAG07868.1|AE004862_2
                           (AE004862) rod shape-determining protein MreC [Pseudomonas aeruginosa PAO1]
                           330
                           99.69
                           99.99
                           0
                           1637.0
     

     
     
116961
                           gb|AAN66559.1|AE016284_7
                           (AE016777) rod shape determining protein MreC [Pseudomonas putida KT2440]
                           342
                           72.80
                           82.45
                           22
                           1213.0
     

     
     
116963
                           gb|AAO57920.1|
                           (AE016872) rod shape-determining protein MreC [Pseudomonas syringae pv. tomato str. DC3000]
                           361
                           67.59
                           77.28
                           33
                           1160.0
     

     
     
116962
                           gb|AAF22468.1|AF063934_2
                           (AF063934) rod shape determining protein MreC [Pseudomonas fluorescens]
                           367
                           66.75
                           76.83
                           38
                           1166.0
     

     






      

          
GC ORFID: 45238How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4480Glimmer Score: 

          
Start: 4439584Stop: 4438592

          
Length: 993


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4480 translation of PA4480Homolog Bit Score 636.0

          
Other Homologs:  psyr_15may02_Scaffold4_revised_gene5505 (ORF: BLASTX 449.0), t_PA4480 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGCTGGCCGTGCTGTCGGTCAGCCTGATGGTGGTGGACGCCCGGTTCGACTATCTGGAGCCCGTCCGCAGC 
AAGCTGGGGATGGTGCTCACGCCTTTCTACGGCCTGGCGGAAATGCCGGTGCGCGCCTGGGAAGGCGTGCGC 
GACCAGTTCAGCAGCCGTAGCGAGCTGATCGCCGAGAACGAACGCCTCAAGGCCGAGTCCCTGCTGATGCAG 
CGCCGGGTGCAGAAACTCGCCGCGCTCACCGAGCAGAACGTGCGCCTGCGCGAGCTGCTCAATTCCGCCGCG 
CTGGTCGACGACAAGGTACTGGTCAGCGAGCTGATCGGCGTCGATCCGAACCCCTTCACCCAACGCATCATG 
ATCGACAAGGGCGAGAACGACGGCGTCTTCGTCGGCCAGCCGGTGCTCGATGCCAGCGGCCTGATGGGCCAG 
GTGGTCGAGGTGATGCCCTATACCGCGCGGGTCCTGCTGCTCACCGACACCACCCACAGTATCCCGGTGCAG 
GTCAATCGCAACGGCCTGCGCGCCATCGCCGTCGGCACCGGCAATCCCGAGCGCTTGGAATTGCGCTACGTC 
GCCGACACCGCCGACATCAAGGAAGGCGACCTACTGGTCAGCTCCGGCCTGGGGCAACGCTTCCCCGCCGGC 
TATCCGGTGGCCACGGTCAAGGAAGTGATCCACGATTCCGGCCAGCCGTTCGCCGTGGTGCGCGCGGTGCCG 
ACCGCGAAGATGAACCGTAGCCGCTACGTGCTGCTGGTGTTCAGCGACAGCCGCACCCCGGAACAGCGCGCC 
AACGACGCCGCCGAGGCCCAGGAAGAGGCGGACAAGAAAGCCGCCGCCGGCGCCCAGGCGCCCCAGCCTGCC 
GCGCAACCGGCCGCCGCGCCGTCGCCGGCCACCCCGGCAGCCCAGGGTGCTGCGCAGCAGCCCGCCGCCGCG 
CCCGCGCCAGCTCCCACGCAGCCTGCCGCTCCGGCGGCGAACGGGGGACGCCGCTGA