ViewGene
Locus Name:
GeneID:
Location:

GeneID:2184
TrivialName: PA14_37810
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_37810
PAO1 Ortholog Locus:PA2064
Sequence Length:1056
Protein Length:351
Start: 3371040
Stop: 3372095
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05452.1|AE004633_3 (AE004633) copper resistance protein B precursor [Pseudomonas aeruginosa PAO1]
Identities = 320/351 (91%), Positives = 322/351 (91%), Gaps = 26/351 (7%)
Sequence:
ATGAGCCGCCAGCCGAAACCCGCCCGCCTGGCCGGCGGTGCCCTCGCCCTGGGTCTGCTGCTGGGCCTGCCG
AATCCCGTCGAGGCCGCGCAGGACAATGGCATGGAGCACTCGGCCATGGACCACGGCGCGATGCCTGGCATG
GACCACGGCCAGAGGACCTCCGCCCCGGCGAAACCCATGGACCACCAGGGGATGGGGCATGGCCGGATGCCG
GGGATGGACCACGGCGCCGCATCGCCCGCCCCGGCACAGCCCATGGAGCACCAGGGAATGGACCATGGCCGG
ATGCCGGGGATGAGCCACGCTGCGCGGCAACCTCCGGGGCAGGCCGCGCCCAGCCAGAGCCGTACGCCGATC
CCGGTGCCGACCGAGGCCGACCGCCGTGCCGCCTTTCCGCCGCTGGAGGGGCACAAGGTGCACGACAGCGCG
CTGAACAGCTTCTTCCTGCTCGACCAGTTGGAATACCAGGACGCCGACGACGGCAGCGCGCTGGCCTGGGAC
GCCAGCGGCTGGATCGGCGGCGACATCAACCGCCTGTGGCTGCGCAGCGAAGGCGAACGGCTCGACGGCAAG
ACCGAGGACGCCGAGGTCCAGGCGCTGTTCGGGCACGCCATCGGTCCCTGGTGGGACCTGGTCGCCGGCGTG
CGCCAGGATTTCAAGCCCGGCTCGCCGCAGACCTGGGCGGCGTTCGGCGTGCAGGGCCTGGCGCTCTACGAC
TTCGAGGCGGAAGTCACCGCCTTCCTCGGCGAGAACGGCCAGAGCGCGCTGCGCCTGGAGGGCGAGTACGAC
ATCCTGTTGACCAACCGGCTGATCCTCCAGCCCAGCGCCGAGGTCAACCTGTACGGCAGGAGCGATCCCGCC
CGCGGCATCGGTTCGGGCCTTGCCGATAGCGAGCTGGGCCTGCGCCTGCGCTACGAGATCCGTCGCGAGTTC
GCGCCCTACATCGGGGTGACCTGGAACCGCTCCTACGGCAACAGCGCCGACCTGGCCCGCGCCGAGGGCGAG
GACGACGACGAGGCGCGCTTCGTCGCCGGTATCCGCATGTGGTTCTGA
Translation:
MSRQPKPARLAGGALALGLLLGLPNPVEAAQDNGMEHSAMDHGAMPGMDHGQRTSAPAKPMDHQGMGHGRMP
GMDHGAASPAPAQPMEHQGMDHGRMPGMSHAARQPPGQAAPSQSRTPIPVPTEADRRAAFPPLEGHKVHDSA
LNSFFLLDQLEYQDADDGSALAWDASGWIGGDINRLWLRSEGERLDGKTEDAEVQALFGHAIGPWWDLVAGV
RQDFKPGSPQTWAAFGVQGLALYDFEAEVTAFLGENGQSALRLEGEYDILLTNRLILQPSAEVNLYGRSDPA
RGIGSGLADSELGLRLRYEIRREFAPYIGVTWNRSYGNSADLARAEGEDDDEARFVAGIRMWF*
AnnotationID:2192GeneID:2184
AnnotatorUID: diggins
Modification Date/Time: 2005-03-03 06:06:54
Gene Name:pcoB
Confidence Code:2
GeneProduct:copper resistance protein B precursor
Cell Localization:()
Synonyms:copB
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(1) Adaptation, protection
Alternate Gene Product Name:copper-binding protein
Functional Category Confidence Code:5
COGs:COG3667,pfam05275
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70944.1|AE016738_6 (AE016794) copper resistance protein B [Pseudomonas putida KT2440]
          Length = 369

 Score =  452 bits (1164), Expect = e-126
 Identities = 230/352 (65%), Positives = 270/352 (76%), Gaps = 31/352 (8%)

>emb|CAA58526.1| (X83541) pcoB [Escherichia coli]
          Length = 296

 Score =  338 bits (866), Expect = 4e-92
 Identities = 159/274 (58%), Positives = 203/274 (74%)








 
Structural Features:
COG3667, PcoB, Uncharacterized protein involved in copper resistance 
[Inorganic ion transport and metabolism]
CD-Length = 321 residues, 100.0% aligned
Score =  414 bits (1066), Expect = 6e-117
Identities = 185/351 (52%), Positives = 218/351 (62%), Gaps = 30/351 (8%)

pfam05275, CopB, Copper resistance protein B precursor (CopB). 
CD-Length = 245 residues, 100.0% aligned
Score =  331 bits (851), Expect = 6e-92
Identities = 148/245 (60%), Positives = 183/245 (74%)






   
Genomic Context:






   
Comment:


















    ReferenceID:4371




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21517




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32638




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2184_cdd.html


    		




 
URL:

















    ReferenceID:34408




  
Author/Investigator(s):
        Riise E, Molin S.





  
Title:
        Purification and characterization of the CopB replication control protein, and precise mapping of its target site in the R1 plasmid.




  
PubMed:
        3520615


MedLine:
        




    
Source:
Plasmid. 1986 May;15(3):163-71.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3520615

















    ReferenceID:34409




  
Author/Investigator(s):
        Cha JS, Cooksey DA.





  
Title:
        Copper resistance in Pseudomonas syringae mediated by periplasmic and outer membrane proteins.




  
PubMed:
        1924351


MedLine:
        




    
Source:
Proc Natl Acad Sci U S A. 1991 Oct 15;88(20):8915-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1924351

















    ReferenceID:34410




  
Author/Investigator(s):
        Silver S, Ji G.





  
Title:
        Newer systems for bacterial resistances to toxic heavy metals.




  
PubMed:
        7843081


MedLine:
        




    
Source:
Environ Health Perspect. 1994 Sep;102 Suppl 3:107-13.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7843081









           
Homologs By Global Alignment 

           

       



     
Gene ID:2184
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3727
                           PA2064_tr
                           translation of PA2064
                           351
                           91.45
                           92.02
                           26
                           1681.0
     

     
     
114689
                           gb|AAG05452.1|AE004633_3
                           (AE004633) copper resistance protein B precursor [Pseudomonas aeruginosa PAO1]
                           351
                           91.45
                           92.02
                           26
                           1681.0
     

     
     
114691
                           gb|AAA25807.1|
                           (M19930) copB peptide [Pseudomonas syringae]
                           364
                           59.89
                           70.32
                           49
                           1115.0
     

     
     
114690
                           gb|AAN70944.1|AE016738_6
                           (AE016794) copper resistance protein B [Pseudomonas putida KT2440]
                           395
                           59.74
                           70.12
                           70
                           1192.0
     

     
     
114693
                           dbj|BAA85308.1|
                           (AB033419) ORFB [Pseudomonas syringae]
                           355
                           57.74
                           68.16
                           54
                           1041.0
     

     






      

          
GC ORFID: 45186How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2064Glimmer Score: 

          
Start: 1582873Stop: 1581818

          
Length: 1056


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2064 translation of PA2064Homolog Bit Score 644.0

          
Other Homologs:  psyr_15may02_Scaffold10_revised_gene1698 (ORF: BLASTX 409.0), psyr_15may02_Scaffold10_revised_gene1539 (ORF: BLASTX 383.0), t_PA2064 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCCGCCAGCCGAAACCCGCCCGCCTGGCCGGCGGTGCCCTCGCCCTGGGTCTGCTGCTGGGCCTGCCG 
AATCCCGTCGAGGCCGCGCAGGACAATGGCATGGAGCACTCGGCCATGGACCACGGCGCGATGCCTGGCATG 
GACCACGGCCAGAGGACCTCCGCCCCGGCGAAACCCATGGACCACCAGGGGATGGGGCATGGCCGGATGCCG 
GGGATGGACCACGGCGCCGCATCGCCCGCCCCGGCACAGCCCATGGAGCACCAGGGAATGGACCATGGCCGG 
ATGCCGGGGATGAGCCACGCTGCGCGGCAACCTCCGGGGCAGGCCGCGCCCAGCCAGAGCCGTACGCCGATC 
CCGGTGCCGACCGAGGCCGACCGCCGTGCCGCCTTTCCGCCGCTGGAGGGGCACAAGGTGCACGACAGCGCG 
CTGAACAGCTTCTTCCTGCTCGACCAGTTGGAATACCAGGACGCCGACGACGGCAGCGCGCTGGCCTGGGAC 
GCCAGCGGCTGGATCGGCGGCGACATCAACCGCCTGTGGCTGCGCAGCGAAGGCGAACGGCTCGACGGCAAG 
ACCGAGGACGCCGAGGTCCAGGCGCTGTTCGGGCACGCCATCGGTCCCTGGTGGGACCTGGTCGCCGGCGTG 
CGCCAGGATTTCAAGCCCGGCTCGCCGCAGACCTGGGCGGCGTTCGGCGTGCAGGGCCTGGCGCTCTACGAC 
TTCGAGGCGGAAGTCACCGCCTTCCTCGGCGAGAACGGCCAGAGCGCGCTGCGCCTGGAGGGCGAGTACGAC 
ATCCTGTTGACCAACCGGCTGATCCTCCAGCCCAGCGCCGAGGTCAACCTGTACGGCAGGAGCGATCCCGCC 
CGCGGCATCGGTTCGGGCCTTGCCGATAGCGAGCTGGGCCTGCGCCTGCGCTACGAGATCCGTCGCGAGTTC 
GCGCCCTACATCGGGGTGACCTGGAACCGCTCCTACGGCAACAGCGCCGACCTGGCCCGCGCCGAGGGCGAG 
GACGACGACGAGGCGCGCTTCGTCGCCGGTATCCGCATGTGGTTCTGA