ViewGene
Locus Name:
GeneID:
Location:

GeneID:2060
TrivialName: PA14_25300
AnnotatorUID: danlee
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_25300
PAO1 Ortholog Locus:
Sequence Length:1245
Protein Length:414
Start: 2213186
Stop: 2214429
Strand: +
Type:
ChromosomeID:2
Status: INACTIVE
Frame Discrepancy: TRUE
Missense Discrepancy:TRUE
Comments:
Inactivated 1/02/06. PA14_25300 and PA14_25310 replaced by PA14_25305 (GeneID 7375). Homology: gb|AAG06386.1|AE004724_15 (AE004724) Na+-translocating NADH:ubiquinone oxidoreductase subunit Nrq2 [Pseudomonas aeruginosa PAO1]
Identities = 324/324 (100%), Positives = 324/324 (100%)
Sequence:
ATGGGTTTGCGTAACCTGCTCGACAAGGTCGAGCATCATTTCGAGAAGGGCGGGCGCTACGAGAAGTGGTAC
CCGCTGTACGAAGCCGTGGACACCTTCCTCTATCGTCCCGGCAGCGTCACCCGCACCACTGCACACGTGCGC
GACGGCATCGACCTGAAGCGCATGATGATCATCGTCTGGCTGTGCACCTTCCCGGCGATGTTCTTTGGCATG
TACAACGTCGGCCACCAGGCCAACCTGATCTTCGCCCAGAGCCCCGACCTGTTGAGCGCCCAGGATGGCTGG
CGCTTCGCCCTGATCGGCGCGCTGGCCGGGTTCGACCCGAACAGCCTGTGGGATTGCCTGGTCCAGGGCGCC
GCGTACTTCCTGCCGGTGTACCTGACCACCTTCATCGTCGGCGGTTTCTGGGAGGTGCTGTTCGCCAGCATC
CGCCGCCACGAGGTCAACGAAGGCTTCTTCGTCACCTCGGTGCTGTTCGCCCTGACCCTGCCGCCGTCCGTC
CCGCTGTGGCAGGTAGCCCTGGGGATCAGCTTCGGCGTGGTGCTGGGCAAGGAAGTGTTCGGCGGCACCGGG
AAGAACTTCCTCAATCCGGCACTGGTCGGCCGGGCCTTCCTGTTCTTCGCCTACCCGGCGCAGATGTCCGGC
GACGCGGTATGGACCTCGGTGGACGGCTTCGCCGGCGCCACCTCGCTGAGCCTGGCCGCCGCCGGCGGGGTG
GACAACATCCTCGGCCACGGCCTGACCTGGATGGATGCCTTCCTCGGCCACATGCAGGGTTCGATGGGCGAG
ACCAGCACCCTGGCGATCTTCATCGGCGGCGCCGTGCTGCTGCTGACCCGCATCGCCTCCTGGCGCATCGTC
GCCGGGGTGATGCTGGGGATGGTCGCCATGAGCTACCTGTTCAACGCCATCGGCTCGGCCAGCAACCCGATG
TTCGCCATGCCCTGGTATTGGCACCTGGTCACcGGGCGGCTTCGCCTTTGGCATGATCTTCATGGCCACCGA
CCCGGTCTCGGCGTCCATGACCGATACCGGCAAGTGGCTGTTCGGTGCCCTGATCGGAGTGATGGTCATGCT
GATCCGGGTGGTCAACCCGGCGTTCCCGGAAGGCATGATGCTGGCGATCCTGTTCGCCAACCTGTTCGCACC
GCTGATCGACCATTTCGTCGTTCAGGCCAACATCAAGCGGAGGCTGGCGCGCAATGGCTAATCAAGAATCCA
CCACCCGCACACTGCTGGTAG
Translation:
MGLRNLLDKVEHHFEKGGRYEKWYPLYEAVDTFLYRPGSVTRTTAHVRDGIDLKRMMIIVWLCTFPAMFFGM
YNVGHQANLIFAQSPDLLSAQDGWRFALIGALAGFDPNSLWDCLVQGAAYFLPVYLTTFIVGGFWEVLFASI
RRHEVNEGFFVTSVLFALTLPPSVPLWQVALGISFGVVLGKEVFGGTGKNFLNPALVGRAFLFFAYPAQMSG
DAVWTSVDGFAGATSLSLAAAGGVDNILGHGLTWMDAFLGHMQGSMGETSTLAIFIGGAVLLLTRIASWRIV
AGVMLGMVAMSYLFNAIGSASNPMFAMPWYWHLVTGRLRLWHDLHGHRPGLGVHDRYRQVAVRCPDRSDGHA
DPGGQPGVPGRHDAGDPVRQPVRTADRPFRRSGQHQAEAGAQWLIKNPPPAHCW*
AnnotationID:2068GeneID:2060
AnnotatorUID: danlee
Modification Date/Time: 2006-02-01 13:01:34
Gene Name:
Confidence Code:4
GeneProduct:
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:()
Alternate Gene Product Name:NADH-ubiquinone oxidoreductase subunit B
Functional Category Confidence Code:5
COGs:COG1805
Secondary Functional Category(ies):
EC Number:1.6.5.-
Status:INACTIVE
Pathway:






 
Homology:
>emb|CAB84034.1| (AL162754) putative Na(+)-translocating NADH-ubiquinone reductase
           subunit B [Neisseria meningitidis Z2491]
          Length = 410

 Score =  434 bits (1116), Expect = e-121
 Identities = 214/333 (64%), Positives = 260/333 (78%), Gaps = 12/333 (3%)

>emb|CAC92474.1| (AJ414156) NADH-ubiquinone oxidoreductase subunit B [Yersinia
           pestis CO92]
          Length = 413

 Score =  431 bits (1108), Expect = e-120
 Identities = 205/334 (61%), Positives = 256/334 (76%), Gaps = 10/334 (2%)

>dbj|BAC60613.1| (AP005081) sodium-translocating NADH-quinone reductase, subunit B
           [Vibrio parahaemolyticus]
          Length = 414

 Score =  417 bits (1071), Expect = e-116
 Identities = 198/334 (59%), Positives = 251/334 (75%), Gaps = 10/334 (2%)








 
Structural Features:
COG1805, NqrB, Na+-transporting NADH:ubiquinone oxidoreductase, 
subunit NqrB 
[Energy production and conversion]
CD-Length = 400 residues,  81.2% aligned
Score =  422 bits (1087), Expect = 3e-119
Identities = 196/327 (59%), Positives = 239/327 (73%), Gaps = 6/327 (1%)






   
Genomic Context:






   
Comment:
Inactivated 1/02/06. PA14_25300 and PA14_25310 replaced by PA14_25305 (GeneID 7375).


















    ReferenceID:4123




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21269




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36056




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2060_cdd.html


    		




 
URL:

















    ReferenceID:36459




  
Author/Investigator(s):
        Hayashi M, Hirai K, Unemoto T.





  
Title:
        Sequencing and the alignment of structural genes in the nqr operon encoding the Na(+)-translocating NADH-quinone reductase from Vibrio alginolyticus.




  
PubMed:
        7729558


MedLine:
        




    
Source:
FEBS Lett. 1995 Apr 17;363(1-2):75-7.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=95246889&dopt=Abstract

















    ReferenceID:36466




  
Author/Investigator(s):
        Beattie P, Tan K, Bourne RM, Leach D, Rich PR, Ward FB.





  
Title:
        Cloning and sequencing of four structural genes for the Na(+)-translocating NADH-ubiquinone oxidoreductase of Vibrio alginolyticus.




  
PubMed:
        7805867


MedLine:
        




    
Source:
FEBS Lett. 1994 Dec 19;356(2-3):333-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=95104445&dopt=Abstract

















    ReferenceID:36471




  
Author/Investigator(s):
        Hayashi M, Hirai K, Unemoto T.





  
Title:
        Cloning of the Na(+)-translocating NADH-quinone reductase gene from the marine bacterium Vibrio alginolyticus and the expression of the beta-subunit in Escherichia coli.




  
PubMed:
        7805866


MedLine:
        




    
Source:
FEBS Lett. 1994 Dec 19;356(2-3):330-2.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=95104444&dopt=Abstract









           
Homologs By Global Alignment 

           

       



     
Gene ID:2060
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3558
                           PA2998_tr
                           translation of PA2998
                           469
                           70.57
                           71.21
                           121
                           1685.0
     

     
     
109683
                           gb|AAG06386.1|AE004724_15
                           (AE004724) Na+-translocating NADH:ubiquinone oxidoreductase subunit Nrq2 [Pseudomonas aeruginosa PAO1]
                           469
                           70.57
                           71.21
                           121
                           1685.0
     

     
     
109684
                           emb|CAE13491.1|
                           (BX571862) Na(+)-translocating NADH-quinone reductase, chain B [Photorhabdus luminescens subsp. laumondii TTO1]
                           471
                           49.25
                           58.81
                           116
                           1140.0
     

     
     
109685
                           emb|CAB84034.1|
                           (AL162754) putative Na(+)-translocating NADH-ubiquinone reductase subunit B [Neisseria meningitidis Z2491]
                           472
                           47.45
                           58.89
                           120
                           1104.0
     

     
     
109686
                           gb|AAF40996.1|
                           (AE002412) Na(+)-translocating NADH-quinone reductase, subunit B [Neisseria meningitidis MC58]
                           473
                           47.14
                           58.77
                           122
                           1101.0
     

     






      

          
GC ORFID: 45062How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2998Glimmer Score: 

          
Start: 2740740Stop: 2739496

          
Length: 1245


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA2998 translation of PA2998Homolog Bit Score 666.0

          
Other Homologs:  t_PA2998 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGGTTTGCGTAACCTGCTCGACAAGGTCGAGCATCATTTCGAGAAGGGCGGGCGCTACGAGAAGTGGTAC 
CCGCTGTACGAAGCCGTGGACACCTTCCTCTATCGTCCCGGCAGCGTCACCCGCACCACTGCACACGTGCGC 
GACGGCATCGACCTGAAGCGCATGATGATCATCGTCTGGCTGTGCACCTTCCCGGCGATGTTCTTTGGCATG 
TACAACGTCGGCCACCAGGCCAACCTGATCTTCGCCCAGAGCCCCGACCTGTTGAGCGCCCAGGATGGCTGG 
CGCTTCGCCCTGATCGGCGCGCTGGCCGGGTTCGACCCGAACAGCCTGTGGGATTGCCTGGTCCAGGGCGCC 
GCGTACTTCCTGCCGGTGTACCTGACCACCTTCATCGTCGGCGGTTTCTGGGAGGTGCTGTTCGCCAGCATC 
CGCCGCCACGAGGTCAACGAAGGCTTCTTCGTCACCTCGGTGCTGTTCGCCCTGACCCTGCCGCCGTCCGTC 
CCGCTGTGGCAGGTAGCCCTGGGGATCAGCTTCGGCGTGGTGCTGGGCAAGGAAGTGTTCGGCGGCACCGGG 
AAGAACTTCCTCAATCCGGCACTGGTCGGCCGGGCCTTCCTGTTCTTCGCCTACCCGGCGCAGATGTCCGGC 
GACGCGGTATGGACCTCGGTGGACGGCTTCGCCGGCGCCACCTCGCTGAGCCTGGCCGCCGCCGGCGGGGTG 
GACAACATCCTCGGCCACGGCCTGACCTGGATGGATGCCTTCCTCGGCCACATGCAGGGTTCGATGGGCGAG 
ACCAGCACCCTGGCGATCTTCATCGGCGGCGCCGTGCTGCTGCTGACCCGCATCGCCTCCTGGCGCATCGTC 
GCCGGGGTGATGCTGGGGATGGTCGCCATGAGCTACCTGTTCAACGCCATCGGCTCGGCCAGCAACCCGATG 
TTCGCCATGCCCTGGTATTGGCACCTGGTCACcGGGCGGCTTCGCCTTTGGCATGATCTTCATGGCCACCGA 
CCCGGTCTCGGCGTCCATGACCGATACCGGCAAGTGGCTGTTCGGTGCCCTGATCGGAGTGATGGTCATGCT 
GATCCGGGTGGTCAACCCGGCGTTCCCGGAAGGCATGATGCTGGCGATCCTGTTCGCCAACCTGTTCGCACC 
GCTGATCGACCATTTCGTCGTTCAGGCCAACATCAAGCGGAGGCTGGCGCGCAATGGCTAATCAAGAATCCA 
CCACCCGCACACTGCTGGTAG