ViewGene
Locus Name:
GeneID:
Location:

GeneID:2056
TrivialName: PA14_03700
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_03700
PAO1 Ortholog Locus:PA0283
Sequence Length:999
Protein Length:332
Start: 331944
Stop: 330946
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03672.1|AE004466_6 (AE004466) sulfate-binding protein precursor [Pseudomonas aeruginosa PAO1]
Identities = 332/332 (100%), Positives = 332/332 (100%)
Sequence:
ATGTCCCTTCGTCGTTTCGCCCTGGCCGCACTCGCCAGCGCCCTGGTCTCCGGCCCGGTTGCCGCGGCCACG
CAACTGCTGAACGTTTCCTACGACCCGACCCGCGAGCTGTACCAGGCCTACAACGCGGCCTTCATCAAGCAC
TGGAAGGCCCAGGGCGGCGAGGACCTGACCGTCCAGCAGTCCCACGGAGGCTCCGGCAAGCAGGCCCGCGCG
GTGATCGACGGGCTCAAGGCCGACGTGGTGACCCTGGCCCTGGCCGGCGACATCGACGAACTGCACAAGCTC
GGCAAGCTGCTGCCGGCCGACTGGCAGGCACGCCTGCCGGACAACAGCACCCCCTACACCTCGACCATCGTG
TTCCTGGTGCGCAAGGGCAACCCCAAGGGAATCAAGGACTGGGGCGACCTGACCAAGGAAGGCGTGGAAGTC
ATCACGCCGAACCCGAAGACCTCCGGCGGCGCGCGCTGGAACTTCCTCGCCGCCTGGGCCTGGGCGAAGAAG
CAGTACGGCAGCGACGAGAAGGCCAAGGACTACGTGCAGGCGCTGTACAAGCACGTACCGGTGCTCGACACC
GGCGCCCGCGGCTCGACCATCACCTTCGTCAACAACCAGATCGGCGACGTGCTGCTGGCCTGGGAGAACGAG
GCGTTCCTGGCGAAGAAGGAACAGGGCGGGGAAAACTTCGAGATCGTCGTACCCTCCATCTCCATCCTCGCC
GAACCACCGGTCGCGGTGGTCGACAAGGTGGTCGAGAAGAAAGGCACCCGCAAGGTCGCCGAGGCCTACCTG
CAATACCTGTACAGCGAGGAAGGCCAGCGCATCGCCGCGCAGAACTTCTACCGGCCGCGCAACCAGAAGGTC
GCCGCCGAGTTCGCCACGCAGTTCCCGAAACTGAACCTGGTCACCGTCGACTCCGATTTCGGCGGCTGGAAG
ACGGCCCAGCCGAAGTTCTTCAACGACGGGGGCATCTTCGACCAGATCTACCAGGCGCAATGA
Translation:
MSLRRFALAALASALVSGPVAAATQLLNVSYDPTRELYQAYNAAFIKHWKAQGGEDLTVQQSHGGSGKQARA
VIDGLKADVVTLALAGDIDELHKLGKLLPADWQARLPDNSTPYTSTIVFLVRKGNPKGIKDWGDLTKEGVEV
ITPNPKTSGGARWNFLAAWAWAKKQYGSDEKAKDYVQALYKHVPVLDTGARGSTITFVNNQIGDVLLAWENE
AFLAKKEQGGENFEIVVPSISILAEPPVAVVDKVVEKKGTRKVAEAYLQYLYSEEGQRIAAQNFYRPRNQKV
AAEFATQFPKLNLVTVDSDFGGWKTAQPKFFNDGGIFDQIYQAQ*
AnnotationID:2064GeneID:2056
AnnotatorUID: danlee
Modification Date/Time: 2006-02-03 15:03:41
Gene Name:sbp
Confidence Code:2
GeneProduct:sulfate-binding protein precursor
Cell Localization:(8) Periplasmic
Synonyms:
Cell Localization Confidence Code:2
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:sulfate starvation-induced protein 2
Functional Category Confidence Code:5
COGs:COG1613,COG4150
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70736.1|AE016717_4 (AE016793) sulfate ABC transporter, periplasmic sulfate-binding
           protein [Pseudomonas putida KT2440]
          Length = 342

 Score =  519 bits (1337), Expect = e-147
 Identities = 261/335 (77%), Positives = 291/335 (86%), Gaps = 3/335 (0%)

>gb|AAO53853.1| (AE016857) sulfate-binding protein [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 338

 Score =  503 bits (1295), Expect = e-142
 Identities = 253/338 (74%), Positives = 286/338 (84%), Gaps = 6/338 (1%)

>gb|AAL41834.1| (AE009049) ABC transporter, substrate binding protein [sulfate]
           [Agrobacterium tumefaciens str. C58 (U. Washington)]
          Length = 402

 Score =  432 bits (1111), Expect = e-120
 Identities = 214/316 (67%), Positives = 250/316 (79%), Gaps = 3/316 (0%)






 
Structural Features:
COG1613, Sbp, ABC-type sulfate transport system, periplasmic component 
[Inorganic ion transport and metabolism]
CD-Length = 348 residues,  92.5% aligned
Score =  537 bits (1384), Expect = 1e-153
Identities = 232/322 (72%), Positives = 265/322 (82%), Gaps = 1/322 (0%)

COG4150, CysP, ABC-type sulfate transport system, periplasmic component 
[Inorganic ion transport and metabolism]
CD-Length = 341 residues,  98.5% aligned
Score =  378 bits (972), Expect = 5e-106
Identities = 167/336 (49%), Positives = 224/336 (66%), Gaps = 6/336 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:4115




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21261




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33635




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2056_cdd.html


    		




 
URL:

















    ReferenceID:35439




  
Author/Investigator(s):
        Hellinga HW, Evans PR.





  
Title:
        Nucleotide sequence and high-level expression of the major Escherichia coli phosphofructokinase.




  
PubMed:
        3158524


MedLine:
        




    
Source:
Eur J Biochem. 1985 Jun 3;149(2):363-73.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3158524

















    ReferenceID:35440




  
Author/Investigator(s):
        Hryniewicz M, Sirko A, Palucha A, Bock A, Hulanicka D.





  
Title:
        Sulfate and thiosulfate transport in Escherichia coli K-12: identification of a gene encoding a novel protein involved in thiosulfate binding.




  
PubMed:
        2188959


MedLine:
        




    
Source:
J Bacteriol. 1990 Jun;172(6):3358-66.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2188959

















    ReferenceID:35441




  
Author/Investigator(s):
        Mansilla MC, de Mendoza D.





  
Title:
        The Bacillus subtilis cysP gene encodes a novel sulphate permease related to the inorganic phosphate transporter (Pit) family.




  
PubMed:
        10784039


MedLine:
        




    
Source:
Microbiology. 2000 Apr;146 ( Pt 4):815-21.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10784039









           
Homologs By Global Alignment 

           

       



     
Gene ID:2056
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3552
                           PA0283_tr
                           translation of PA0283
                           332
                           99.99
                           99.99
                           0
                           1722.0
     

     
     
109543
                           gb|AAG03672.1|AE004466_6
                           (AE004466) sulfate-binding protein precursor [Pseudomonas aeruginosa PAO1]
                           332
                           99.99
                           99.99
                           0
                           1722.0
     

     
     
109544
                           gb|AAN70736.1|AE016717_4
                           (AE016793) sulfate ABC transporter, periplasmic sulfate-binding protein [Pseudomonas putida KT2440]
                           342
                           76.31
                           85.08
                           10
                           1343.0
     

     
     
109545
                           gb|AAO53853.1|
                           (AE016857) sulfate-binding protein [Pseudomonas syringae pv. tomato str. DC3000]
                           338
                           74.85
                           84.61
                           6
                           1304.0
     

     
     
109557
                           emb|CAE26194.1|
                           (BX572595) sulfate ABC transporter, periplasmic binding protein component [Rhodopseudomonas palustris CGA009]
                           333
                           65.16
                           76.57
                           5
                           1096.0
     

     






      

          
GC ORFID: 45058How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0283Glimmer Score: 

          
Start: 6133285Stop: 6132287

          
Length: 999


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0283 translation of PA0283Homolog Bit Score 667.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene4048 (ORF: BLASTX 508.0), t_PA1493 (ORF: BLASTX 388.0), t_PA0283 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCCCTTCGTCGTTTCGCCCTGGCCGCACTCGCCAGCGCCCTGGTCTCCGGCCCGGTTGCCGCGGCCACG 
CAACTGCTGAACGTTTCCTACGACCCGACCCGCGAGCTGTACCAGGCCTACAACGCGGCCTTCATCAAGCAC 
TGGAAGGCCCAGGGCGGCGAGGACCTGACCGTCCAGCAGTCCCACGGAGGCTCCGGCAAGCAGGCCCGCGCG 
GTGATCGACGGGCTCAAGGCCGACGTGGTGACCCTGGCCCTGGCCGGCGACATCGACGAACTGCACAAGCTC 
GGCAAGCTGCTGCCGGCCGACTGGCAGGCACGCCTGCCGGACAACAGCACCCCCTACACCTCGACCATCGTG 
TTCCTGGTGCGCAAGGGCAACCCCAAGGGAATCAAGGACTGGGGCGACCTGACCAAGGAAGGCGTGGAAGTC 
ATCACGCCGAACCCGAAGACCTCCGGCGGCGCGCGCTGGAACTTCCTCGCCGCCTGGGCCTGGGCGAAGAAG 
CAGTACGGCAGCGACGAGAAGGCCAAGGACTACGTGCAGGCGCTGTACAAGCACGTACCGGTGCTCGACACC 
GGCGCCCGCGGCTCGACCATCACCTTCGTCAACAACCAGATCGGCGACGTGCTGCTGGCCTGGGAGAACGAG 
GCGTTCCTGGCGAAGAAGGAACAGGGCGGGGAAAACTTCGAGATCGTCGTACCCTCCATCTCCATCCTCGCC 
GAACCACCGGTCGCGGTGGTCGACAAGGTGGTCGAGAAGAAAGGCACCCGCAAGGTCGCCGAGGCCTACCTG 
CAATACCTGTACAGCGAGGAAGGCCAGCGCATCGCCGCGCAGAACTTCTACCGGCCGCGCAACCAGAAGGTC 
GCCGCCGAGTTCGCCACGCAGTTCCCGAAACTGAACCTGGTCACCGTCGACTCCGATTTCGGCGGCTGGAAG 
ACGGCCCAGCCGAAGTTCTTCAACGACGGGGGCATCTTCGACCAGATCTACCAGGCGCAATGA