ViewGene
Locus Name:
GeneID:
Location:

GeneID:1834
TrivialName: PA14_63120
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_63120
PAO1 Ortholog Locus:PA4774
Sequence Length:1050
Protein Length:349
Start: 5634070
Stop: 5635119
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08160.1|AE004891_2 (AE004891) hypothetical protein [Pseudomonas aeruginosa PAO1]
Identities = 348/349 (99%), Positives = 348/349 (99%)
Sequence:
GTGCCGGCGGAGGTCGCCGCCGGGTTGCCGCCGCTGCCGCCGAACCACTTCTATTTCTATCCGCCGGACCCG
CTGCTGGAAGGCGACGGCGCGATCACCGCGATCGAGGACAGCGACCCCTTCGACCAGTACGTCTACCGCCTG
CGCCGGGTGCTCTACCAGGGCCGCACGCGCTGGCAGAACGTGCTGATCGCCGACACCTACAACTACGACCGC
GTGCTGATGCTCGACGGCGCGATCCAGAGCGCCGAGAGCGATGAGTCGCTGTACCACGAGCTGCTGGTGCAG
CCGGCCATGCTCGCCCACGACGAGCCGCGCGACGTACTGATCATCGGCGGCGGCGAAGGCGCCACCCTGCGC
GAGGTGCTCTCCCATGCCAGCGTGCGGCGGGCGGTGATGGTCGATCTCGACCGGGAGCTGGTGGAACTGTGT
CGCGAACACCTGTTCCAGTGGCACCAGGGCGCCTTCGACGACCCACGCTGCGAACTGCTCGCCGAGGACGGC
CGGGCCTATCTCGAGCGCGACCCGTCGCTCTATGACGTGGTCATCATCGACGTCGTCGACATGCTCGACAAC
GGCCCGGCCCAGGCTCTCTATACCCGCCAGTTCTACGAGCTGCTGCATTCGCGCCTGCGACCGGGCGGGGTG
GTCGCGGTGCAGGGGCTGGAGTTCTCCCACAGCGACGACAAGCCCCACGCGGCCCTCGCCCGTACCCTGCGC
AGTGTCTTCTCCCAGGTCCACAGCTACCGCGCCACGGTGCCCTCGTTCCTCTCCTCCTGGGGCTTCCTGCTG
GCTTCCGACTGGCTCGACGCGAACCACTGGCAGGCCGAGGACATCGACCGACGCATCGAACGCAAGCTCGGC
CCGCTGTGGCTCGACCACCTCGACGGCGACTACCTCAAGGCCTGCTTCGTGATGGACCGGGAAACCCGCTTC
CTGCTCGCCCAGCCGGGACCGGTACTGGAAGACGGCGTACCCTTCGTCGCACCGCCGGATATCGAGGAGATC
GAGTTCGGCCCGGCACAATTGCCGGCCCTGGCGAGGACCTGA
Translation:
MPAEVAAGLPPLPPNHFYFYPPDPLLEGDGAITAIEDSDPFDQYVYRLRRVLYQGRTRWQNVLIADTYNYDR
VLMLDGAIQSAESDESLYHELLVQPAMLAHDEPRDVLIIGGGEGATLREVLSHASVRRAVMVDLDRELVELC
REHLFQWHQGAFDDPRCELLAEDGRAYLERDPSLYDVVIIDVVDMLDNGPAQALYTRQFYELLHSRLRPGGV
VAVQGLEFSHSDDKPHAALARTLRSVFSQVHSYRATVPSFLSSWGFLLASDWLDANHWQAEDIDRRIERKLG
PLWLDHLDGDYLKACFVMDRETRFLLAQPGPVLEDGVPFVAPPDIEEIEFGPAQLPALART*
AnnotationID:1843GeneID:1834
AnnotatorUID: diggins
Modification Date/Time: 2005-05-23 08:08:24
Gene Name:
Confidence Code:3
GeneProduct:putative spermidine synthase
Cell Localization:()
Synonyms:PA4774
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0421,pfam01564
Secondary Functional Category(ies):
EC Number:2.5.1.16
Status:ACTIVE
Pathway:






 
Homology:
>dbj|BAA79745.1| (AP000060) 306aa long hypothetical spermidine synthase [Aeropyrum
           pernix]
          Length = 306

 Score =  220 bits (561), Expect = 9e-57
 Identities = 109/218 (50%), Positives = 148/218 (67%), Gaps = 2/218 (0%)

>gb|AAL63322.1| (AE009809) spermidine synthase [Pyrobaculum aerophilum str. IM2]
          Length = 291

 Score =  181 bits (459), Expect = 6e-45
 Identities = 103/260 (39%), Positives = 153/260 (58%), Gaps = 4/260 (1%)







 
Structural Features:
COG0421, SpeE, Spermidine synthase 
[Amino acid transport and metabolism] 
CD-Length = 282 residues,  95.0% aligned
Score =  241 bits (616), Expect = 1e-64
Identities = 102/275 (37%), Positives = 156/275 (56%), Gaps = 8/275 (2%)

pfam01564, Spermine_synth, Spermine/spermidine synthase.
CD-Length = 239 residues,  97.5% aligned
Score =  185 bits (471), Expect = 8e-48
Identities = 81/243 (33%), Positives = 138/243 (56%), Gaps = 17/243 (6%)






   
Genomic Context:






   
Comment:


















    ReferenceID:14441




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20818




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37682




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1834_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:1834
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3218
                           PA4774_tr
                           translation of PA4774
                           349
                           99.71
                           99.71
                           0
                           1842.0
     

     
     
99827
                           gb|AAG08160.1|AE004891_2
                           (AE004891) hypothetical protein PA4774 [Pseudomonas aeruginosa PAO1]
                           349
                           99.42
                           99.71
                           0
                           1839.0
     

     
     
99828
                           dbj|BAA79745.1|
                           (AP000060) 306aa long hypothetical spermidine synthase [Aeropyrum pernix]
                           381
                           34.64
                           46.19
                           107
                           550.0
     

     
     
99829
                           gb|AAC14108.1|
                           (AF055854) spermidine synthase [Synechococcus sp. PCC 7002]
                           361
                           14.68
                           21.32
                           248
                           254.0
     

     






      

          
GC ORFID: 44836How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4774Glimmer Score: 

          
Start: 4897750Stop: 4898799

          
Length: 1050


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4774 translation of PA4774Homolog Bit Score 714.0

          
Other Homologs:  t_PA4774 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGCCGGCGGAGGTCGCCGCCGGGTTGCCGCCGCTGCCGCCGAACCACTTCTATTTCTATCCGCCGGACCCG 
CTGCTGGAAGGCGACGGCGCGATCACCGCGATCGAGGACAGCGACCCCTTCGACCAGTACGTCTACCGCCTG 
CGCCGGGTGCTCTACCAGGGCCGCACGCGCTGGCAGAACGTGCTGATCGCCGACACCTACAACTACGACCGC 
GTGCTGATGCTCGACGGCGCGATCCAGAGCGCCGAGAGCGATGAGTCGCTGTACCACGAGCTGCTGGTGCAG 
CCGGCCATGCTCGCCCACGACGAGCCGCGCGACGTACTGATCATCGGCGGCGGCGAAGGCGCCACCCTGCGC 
GAGGTGCTCTCCCATGCCAGCGTGCGGCGGGCGGTGATGGTCGATCTCGACCGGGAGCTGGTGGAACTGTGT 
CGCGAACACCTGTTCCAGTGGCACCAGGGCGCCTTCGACGACCCACGCTGCGAACTGCTCGCCGAGGACGGC 
CGGGCCTATCTCGAGCGCGACCCGTCGCTCTATGACGTGGTCATCATCGACGTCGTCGACATGCTCGACAAC 
GGCCCGGCCCAGGCTCTCTATACCCGCCAGTTCTACGAGCTGCTGCATTCGCGCCTGCGACCGGGCGGGGTG 
GTCGCGGTGCAGGGGCTGGAGTTCTCCCACAGCGACGACAAGCCCCACGCGGCCCTCGCCCGTACCCTGCGC 
AGTGTCTTCTCCCAGGTCCACAGCTACCGCGCCACGGTGCCCTCGTTCCTCTCCTCCTGGGGCTTCCTGCTG 
GCTTCCGACTGGCTCGACGCGAACCACTGGCAGGCCGAGGACATCGACCGACGCATCGAACGCAAGCTCGGC 
CCGCTGTGGCTCGACCACCTCGACGGCGACTACCTCAAGGCCTGCTTCGTGATGGACCGGGAAACCCGCTTC 
CTGCTCGCCCAGCCGGGACCGGTACTGGAAGACGGCGTACCCTTCGTCGCACCGCCGGATATCGAGGAGATC 
GAGTTCGGCCCGGCACAATTGCCGGCCCTGGCGAGGACCTGA