ViewGene
Locus Name:
GeneID:
Location:

GeneID:1809
TrivialName: PA14_40470
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_40470
PAO1 Ortholog Locus:
Sequence Length:1455
Protein Length:484
Start: 3616649
Stop: 3615195
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAN69547.1|AE016588_4 (AE016788) potassium uptake protein, TrkH family [Pseudomonas putida KT2440]
Identities = 362/484 (74%), Positives = 405/484 (83%)
Sequence:
ATGAGCTTTTCCCTGCTCCGCCTGGTGGGATTCATCCTCGGGCTTTTCCTGGTCACCCTGGCGGCCAGCATG
CTGATCCCGATGCTGACGCTGGCGCTCTTCCAGCGCTACGACGATTTCGACGCCTTCCTCTGGTCGAGCCTG
CTCACCGCGGCTGCCGGccTCGCGCTGGTCTTGCCGGGACGGCCGGCTCACGTGCATTTTCGTGCCCGGCAC
ATGTATTTCCTGACCACCGCCAGTTGGGTGGTGGTCTGCGCCTTCGCCGCGCTGCCGATGGTGATGGCTCGG
CACATCAGCTACACCGATGCgTtcttcGAGACCATGTCGGGCATCACCACCACCGGCTCCACCGTGTTCAGC
GGGTTGGACCAGATGTCGCCGGGCATCCTCATCTGGCGCTCGTTGTTGCACTGGCTCGGCGGCATCGGTTTC
ATCGGCATGGCGGTGGCGATCCTGCCGCTGCTGCGTGTCGGCGGCATGCGCCTGTTCCAGACCGAATCCTCC
GACTGGGGCGAGAAGGTGATGCCACGCTCGCACATGGCGGCCAAGTACATCCTCGGCGTCTACGTCGCGCTT
ACGCTGCTGGGCGTGCTGTGCTTCTGGTGGGCGGGCATGACCCCCTTCGAGGCGTTCAACCATTCGATGGCC
GCCATCTCCACGGGCGGCTTCTCCACCTCCGACGCTTCCCTCGCGCACTGGCCGCAACCGGCGGTGCACTGG
GTCGCCGTGTTGTTCATGATCCTCGGCAGCCTGCCGTTCACCCTCTATGTCGCGACCCTGCGCGGCCATCGC
CTGGCCTTGCTCAAGGACCACCAGGTGCATGGCTTCATCGGCTTCCTGGCGGTGACCTGGCTGGTTTTCGCC
TTCTGGCTCTGGCTGAATTCCGATTACGGCTGGTGGGATGCGCTGCGCCTGGTGGCGGTCAATGTGACGTCG
GTGGTGACCACCACCGGTTTCGCCCTGACCGACTACACCACCTGGGGAACCTTCGCGGTCATGCTGTTCTTC
TACCTCACCTTCGTCGGCGGTTGCTCGGGCTCCACCGCCGGCGGGTTGAAGATCTTCCGCTTCCAGGTGGCC
TTCGTGTTGCTCAGGGCCAACCTGCAGAACCTCGTGCATCCGCGGGCGACCATCCGGCAAAGCTACAACGGC
CATCCGCTGGACGAGGAAATCGTCCGCTCGCTGATCACCTTTTCCTTCTTCTTCACCATCACCATCGGCGTG
ATCGCACTGGGGTTGACCATGCTCGGCCTCGACTGGGTCTCCGCGTTCACCGGTGCCGCGACCGCCGTGTGC
AACGTCGGGCCCGGGCTCGGGCCGATTATCGGACCGGCGGGAAACTTTTCCAGCCTGCCGGACATGGCCAAG
TGGCTGCTGACCCTCGGCATGCTGCTTGGTCGGCTAGAGATTCTCACCGTACTGGTGCTGTGCACCCGCGCG
TTCTGGCGCTTCTGA
Translation:
MSFSLLRLVGFILGLFLVTLAASMLIPMLTLALFQRYDDFDAFLWSSLLTAAAGLALVLPGRPAHVHFRARH
MYFLTTASWVVVCAFAALPMVMARHISYTDAFFETMSGITTTGSTVFSGLDQMSPGILIWRSLLHWLGGIGF
IGMAVAILPLLRVGGMRLFQTESSDWGEKVMPRSHMAAKYILGVYVALTLLGVLCFWWAGMTPFEAFNHSMA
AISTGGFSTSDASLAHWPQPAVHWVAVLFMILGSLPFTLYVATLRGHRLALLKDHQVHGFIGFLAVTWLVFA
FWLWLNSDYGWWDALRLVAVNVTSVVTTTGFALTDYTTWGTFAVMLFFYLTFVGGCSGSTAGGLKIFRFQVA
FVLLRANLQNLVHPRATIRQSYNGHPLDEEIVRSLITFSFFFTITIGVIALGLTMLGLDWVSAFTGAATAVC
NVGPGLGPIIGPAGNFSSLPDMAKWLLTLGMLLGRLEILTVLVLCTRAFWRF*
AnnotationID:1818GeneID:1809
AnnotatorUID: danlee
Modification Date/Time: 2006-04-12 15:03:40
Gene Name:
Confidence Code:3
GeneProduct:putative potassium uptake protein, TrkH family
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0168,pfam02386
Secondary Functional Category(ies):membrane protein
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN69547.1|AE016588_4 (AE016788) potassium uptake protein, TrkH family [Pseudomonas
           putida KT2440]
          Length = 484

 Score =  744 bits (1922), Expect = 0.0
 Identities = 362/484 (74%), Positives = 405/484 (83%)

>gb|AAO55321.1| (AE016862) potassium uptake protein TrkH [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 484

 Score =  706 bits (1822), Expect = 0.0
 Identities = 337/483 (69%), Positives = 402/483 (83%)

>gb|AAF93220.1| (AE004095) potassium uptake protein TrkH [Vibrio cholerae]
          Length = 481

 Score =  502 bits (1292), Expect = e-141
 Identities = 256/479 (53%), Positives = 321/479 (67%), Gaps = 3/479 (0%)








 
Structural Features:
COG0168, TrkG, Trk-type K+ transport systems, membrane components 
[Inorganic ion transport and metabolism]
CD-Length = 499 residues,  97.6% aligned
Score =  306 bits (784), Expect = 5e-84
Identities = 184/495 (37%), Positives = 260/495 (52%), Gaps = 23/495 (4%)

pfam02386, TrkH, Cation transport protein. 
CD-Length = 331 residues, 100.0% aligned
Score =  148 bits (374), Expect = 2e-36
Identities = 91/343 (26%), Positives = 138/343 (40%), Gaps = 26/343 (7%)






   
Genomic Context:






   
Comment:
This gene is not present in PAO1.  
It is similar to trkH (PA14_22690, GeneID 864 of PA14, and PA3210 of PAO1).  



















    ReferenceID:3621




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20768




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37088




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1809_cdd.html


    		




 
URL:

















    ReferenceID:37785




  
Author/Investigator(s):
        Nakamura T, Yamamuro N, Stumpe S, Unemoto T, Bakker EP.





  
Title:
        Cloning of the trkAH gene cluster and characterization of the Trk K(+)-uptake system of Vibrio alginolyticus.




  
PubMed:
        9720051


MedLine:
        




    
Source:
Microbiology. 1998 Aug;144 ( Pt 8):2281-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9720051









           
Homologs By Global Alignment 

           

       



     
Gene ID:1809
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
98715
                           gb|AAN69547.1|AE016588_4
                           (AE016788) potassium uptake protein, TrkH family [Pseudomonas putida KT2440]
                           484
                           74.79
                           83.67
                           0
                           1922.0
     

     
     
3179
                           PA3210_tr
                           translation of PA3210
                           484
                           70.86
                           82.85
                           0
                           1857.0
     

     
     
98716
                           gb|AAG06598.1|AE004744_13
                           (AE004744) potassium uptake protein TrkH [Pseudomonas aeruginosa PAO1]
                           484
                           70.86
                           82.85
                           0
                           1857.0
     

     
     
98717
                           gb|AAN70081.1|AE016647_11
                           (AE016790) potassium uptake protein, TrkH family [Pseudomonas putida KT2440]
                           484
                           69.83
                           83.47
                           0
                           1843.0
     

     
     
98718
                           gb|AAO55321.1|
                           (AE016862) potassium uptake protein TrkH [Pseudomonas syringae pv. tomato str. DC3000]
                           484
                           69.62
                           83.05
                           0
                           1821.0
     

     






      

          
GC ORFID: 44811How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3210Glimmer Score: 

          
Start: 1337261Stop: 1338715

          
Length: 1455


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3210 translation of PA3210Homolog Bit Score 720.0

          
Other Homologs:  psyr_15may02_Scaffold8_revised_gene7648 (ORF: BLASTX 708.0), t_PA3210 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCTTTTCCCTGCTCCGCCTGGTGGGATTCATCCTCGGGCTTTTCCTGGTCACCCTGGCGGCCAGCATG 
CTGATCCCGATGCTGACGCTGGCGCTCTTCCAGCGCTACGACGATTTCGACGCCTTCCTCTGGTCGAGCCTG 
CTCACCGCGGCTGCCGGccTCGCGCTGGTCTTGCCGGGACGGCCGGCTCACGTGCATTTTCGTGCCCGGCAC 
ATGTATTTCCTGACCACCGCCAGTTGGGTGGTGGTCTGCGCCTTCGCCGCGCTGCCGATGGTGATGGCTCGG 
CACATCAGCTACACCGATGCgTtcttcGAGACCATGTCGGGCATCACCACCACCGGCTCCACCGTGTTCAGC 
GGGTTGGACCAGATGTCGCCGGGCATCCTCATCTGGCGCTCGTTGTTGCACTGGCTCGGCGGCATCGGTTTC 
ATCGGCATGGCGGTGGCGATCCTGCCGCTGCTGCGTGTCGGCGGCATGCGCCTGTTCCAGACCGAATCCTCC 
GACTGGGGCGAGAAGGTGATGCCACGCTCGCACATGGCGGCCAAGTACATCCTCGGCGTCTACGTCGCGCTT 
ACGCTGCTGGGCGTGCTGTGCTTCTGGTGGGCGGGCATGACCCCCTTCGAGGCGTTCAACCATTCGATGGCC 
GCCATCTCCACGGGCGGCTTCTCCACCTCCGACGCTTCCCTCGCGCACTGGCCGCAACCGGCGGTGCACTGG 
GTCGCCGTGTTGTTCATGATCCTCGGCAGCCTGCCGTTCACCCTCTATGTCGCGACCCTGCGCGGCCATCGC 
CTGGCCTTGCTCAAGGACCACCAGGTGCATGGCTTCATCGGCTTCCTGGCGGTGACCTGGCTGGTTTTCGCC 
TTCTGGCTCTGGCTGAATTCCGATTACGGCTGGTGGGATGCGCTGCGCCTGGTGGCGGTCAATGTGACGTCG 
GTGGTGACCACCACCGGTTTCGCCCTGACCGACTACACCACCTGGGGAACCTTCGCGGTCATGCTGTTCTTC 
TACCTCACCTTCGTCGGCGGTTGCTCGGGCTCCACCGCCGGCGGGTTGAAGATCTTCCGCTTCCAGGTGGCC 
TTCGTGTTGCTCAGGGCCAACCTGCAGAACCTCGTGCATCCGCGGGCGACCATCCGGCAAAGCTACAACGGC 
CATCCGCTGGACGAGGAAATCGTCCGCTCGCTGATCACCTTTTCCTTCTTCTTCACCATCACCATCGGCGTG 
ATCGCACTGGGGTTGACCATGCTCGGCCTCGACTGGGTCTCCGCGTTCACCGGTGCCGCGACCGCCGTGTGC 
AACGTCGGGCCCGGGCTCGGGCCGATTATCGGACCGGCGGGAAACTTTTCCAGCCTGCCGGACATGGCCAAG 
TGGCTGCTGACCCTCGGCATGCTGCTTGGTCGGCTAGAGATTCTCACCGTACTGGTGCTGTGCACCCGCGCG 
TTCTGGCGCTTCTGA