ViewGene
Locus Name:
GeneID:
Location:

GeneID:1737
TrivialName: PA14_18760
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_18760
PAO1 Ortholog Locus:PA3523
Sequence Length:1158
Protein Length:385
Start: 1614329
Stop: 1615486
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06911.1|AE004772_9 (AE004772) probable RND efflux membrane fusion protein precursor [Pseudomonas aeruginosa PAO1]
Identities = 382/385 (99%), Positives = 382/385 (99%)
Sequence:
ATGAATCTCAGACACTTCATCCGCATCACCGCGACGCTAGGTGTCGCGGCCCTTATCGCTGGCTGCGGGGAA
TCGGCGCCGCCCGGGGCGGCGAGCGCCCCGCCAAGCGTTCCCGTCGCCGAGGTGGTGGTTCGCCCGGTGACA
CCCTACGCCGAGTTCACCGGCTCGCTGACCGCTGTCGAGCAGGTAGAACTACGGCCGCGCGTCGCCGGCTAC
ATCCAGGACGTTACGGTGCCGGAAGGCCGCCTGGTAGAGAAGGGCCAGCAACTGTTCCTCATCGACCCGCGC
GTGTTCAAGGCGGCGCAGGATGCCGCCAGGGCGCGCCTGCGCGAGGCCGAGGCCGCGGCGTTGCTGGCGCGC
ACCGAACACGAGCGAGCCGAGCTGCTGTATGCGCGGAAGGTCGTCGCCCGGGAGCGGCTCGACAGCGCCATC
GCCTCGCGCAATGCCAGCAAGGCCCAGGTCGATGCGGCCAGGGCCGCCCTCGACGCGGCGCAACTGGATCTC
GGCTTCACGCGGGTGACGGCACCGATCGGCGGGCGTGTCGGGCATATCCAGGTCACCGAGGGCAACTACGTC
ACCAATGGCGTCACCGCGCTGACCAGCATCGTTTCGGTCGATCCGCTGTACGTGTACTTCGATGTCGACGAG
CGCACCTACCTGCAGGCCCTGGCGCCGACCCGCGGCAGGGAAGGCGAGCAGGCCCCCAGGGTCAAGGTGGCG
CTGCTCACCGACGAGTCCTATGGACGAAGCAGTCGCCTCGATTTCCTCGCCAATGCCGCCGACCGCGGGACC
GGCACGGTCCGGGTTCGGGCGGTGGTCGACAACCCGGACGGGCAACTGACGCCGGGGCTGTTCGCCAAGGTC
AGGCTGGAGACCGGCAAGCCTCACGAGCAGGTgctgGTCGCCGACCATTCCATCGGCACCGACCAGGGCCGG
CGCTATGTGCTGGTCGTCGACGAAGGCAACAAGACCCAGTACCGGCCGGTGGAACTCGGCCCGATGGTCGAC
GGCCTGCGGGTCGTCCGCCAGGGCCTGCAGCCGGGCGAGCGCATCGTCGTCAAGGGCCTGGTGCGGCCGGAC
ATGCAGATCACGCCGCTCCTTGCGGAAATCGATGGCACGCCCGTCGACCTGTCGAAGACCGTGGGGGCCGCA
CAATGA
Translation:
MNLRHFIRITATLGVAALIAGCGESAPPGAASAPPSVPVAEVVVRPVTPYAEFTGSLTAVEQVELRPRVAGY
IQDVTVPEGRLVEKGQQLFLIDPRVFKAAQDAARARLREAEAAALLARTEHERAELLYARKVVARERLDSAI
ASRNASKAQVDAARAALDAAQLDLGFTRVTAPIGGRVGHIQVTEGNYVTNGVTALTSIVSVDPLYVYFDVDE
RTYLQALAPTRGREGEQAPRVKVALLTDESYGRSSRLDFLANAADRGTGTVRVRAVVDNPDGQLTPGLFAKV
RLETGKPHEQVLVADHSIGTDQGRRYVLVVDEGNKTQYRPVELGPMVDGLRVVRQGLQPGERIVVKGLVRPD
MQITPLLAEIDGTPVDLSKTVGAAQ*
AnnotationID:1746GeneID:1737
AnnotatorUID: diggins
Modification Date/Time: 2005-07-17 12:12:01
Gene Name:
Confidence Code:3
GeneProduct:putative RND efflux membrane fusion protein precursor
Cell Localization:()
Synonyms:mexE
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:putative RND multidrug efflux membrane fusion protein
Functional Category Confidence Code:5
COGs:pfam00529,COG0845
Secondary Functional Category(ies):membrane protein
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAL19306.1| (AE008711) putative cation efflux pump [Salmonella typhimurium LT2]
          Length = 408

 Score =  344 bits (882), Expect = 6e-94
 Identities = 188/381 (49%), Positives = 249/381 (65%), Gaps = 18/381 (4%)

>gb|AAN69027.1|AE016535_1 (AE016786) multidrug efflux RND membrane fusion protein MexE
           [Pseudomonas putida KT2440]
          Length = 413

 Score =  288 bits (736), Expect = 5e-77
 Identities = 156/350 (44%), Positives = 219/350 (62%), Gaps = 2/350 (0%)

>gb|AAN56486.1|AE015785_9 (AE015785) RND multidrug efflux membrane fusion protein MexE
           [Shewanella oneidensis MR-1]
          Length = 476

 Score =  270 bits (689), Expect = 1e-71
 Identities = 160/367 (43%), Positives = 218/367 (59%), Gaps = 9/367 (2%)







 
Structural Features:
pfam00529, HlyD, HlyD family secretion protein.
CD-Length = 309 residues, 100.0% aligned
Score = 96.3 bits (239), Expect = 5e-21
Identities = 71/310 (22%), Positives = 121/310 (39%), Gaps = 18/310 (5%)

COG0845, AcrA, Membrane-fusion protein 
[Cell envelope biogenesis, outer membrane]
CD-Length = 372 residues,  98.4% aligned
Score =  127 bits (318), Expect = 3e-30
Identities = 124/374 (33%), Positives = 184/374 (49%), Gaps = 12/374 (3%)






   
Genomic Context:






   
Comment:


















    ReferenceID:3477




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20624




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32043




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1737_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:1737
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3051
                           PA3523_tr
                           translation of PA3523
                           385
                           99.22
                           99.22
                           0
                           1898.0
     

     
     
94181
                           gb|AAG06911.1|AE004772_9
                           (AE004772) probable RND efflux membrane fusion protein precursor [Pseudomonas aeruginosa PAO1]
                           385
                           99.22
                           99.22
                           0
                           1898.0
     

     
     
94182
                           gb|AAL19306.1|
                           (AE008711) putative cation efflux pump [Salmonella typhimurium LT2]
                           412
                           46.60
                           62.13
                           31
                           897.0
     

     
     
3052
                           PA2493_tr
                           translation of PA2493
                           414
                           41.30
                           56.76
                           29
                           775.0
     

     
     
94183
                           gb|AAG05881.1|AE004676_13
                           (AE004676) RND multidrug efflux membrane fusion protein MexE precursor [Pseudomonas aeruginosa PAO1]
                           414
                           41.30
                           56.76
                           29
                           775.0
     

     






      

          
GC ORFID: 44739How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3523Glimmer Score: 

          
Start: 3339605Stop: 3338448

          
Length: 1158


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3523 translation of PA3523Homolog Bit Score 735.0

          
Other Homologs:  t_PA3523 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAATCTCAGACACTTCATCCGCATCACCGCGACGCTAGGTGTCGCGGCCCTTATCGCTGGCTGCGGGGAA 
TCGGCGCCGCCCGGGGCGGCGAGCGCCCCGCCAAGCGTTCCCGTCGCCGAGGTGGTGGTTCGCCCGGTGACA 
CCCTACGCCGAGTTCACCGGCTCGCTGACCGCTGTCGAGCAGGTAGAACTACGGCCGCGCGTCGCCGGCTAC 
ATCCAGGACGTTACGGTGCCGGAAGGCCGCCTGGTAGAGAAGGGCCAGCAACTGTTCCTCATCGACCCGCGC 
GTGTTCAAGGCGGCGCAGGATGCCGCCAGGGCGCGCCTGCGCGAGGCCGAGGCCGCGGCGTTGCTGGCGCGC 
ACCGAACACGAGCGAGCCGAGCTGCTGTATGCGCGGAAGGTCGTCGCCCGGGAGCGGCTCGACAGCGCCATC 
GCCTCGCGCAATGCCAGCAAGGCCCAGGTCGATGCGGCCAGGGCCGCCCTCGACGCGGCGCAACTGGATCTC 
GGCTTCACGCGGGTGACGGCACCGATCGGCGGGCGTGTCGGGCATATCCAGGTCACCGAGGGCAACTACGTC 
ACCAATGGCGTCACCGCGCTGACCAGCATCGTTTCGGTCGATCCGCTGTACGTGTACTTCGATGTCGACGAG 
CGCACCTACCTGCAGGCCCTGGCGCCGACCCGCGGCAGGGAAGGCGAGCAGGCCCCCAGGGTCAAGGTGGCG 
CTGCTCACCGACGAGTCCTATGGACGAAGCAGTCGCCTCGATTTCCTCGCCAATGCCGCCGACCGCGGGACC 
GGCACGGTCCGGGTTCGGGCGGTGGTCGACAACCCGGACGGGCAACTGACGCCGGGGCTGTTCGCCAAGGTC 
AGGCTGGAGACCGGCAAGCCTCACGAGCAGGTgctgGTCGCCGACCATTCCATCGGCACCGACCAGGGCCGG 
CGCTATGTGCTGGTCGTCGACGAAGGCAACAAGACCCAGTACCGGCCGGTGGAACTCGGCCCGATGGTCGAC 
GGCCTGCGGGTCGTCCGCCAGGGCCTGCAGCCGGGCGAGCGCATCGTCGTCAAGGGCCTGGTGCGGCCGGAC 
ATGCAGATCACGCCGCTCCTTGCGGAAATCGATGGCACGCCCGTCGACCTGTCGAAGACCGTGGGGGCCGCA 
CAATGA