ViewGene
Locus Name:
GeneID:
Location:

GeneID:1715
TrivialName: PA14_30570
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_30570
PAO1 Ortholog Locus:PA2592
Sequence Length:1104
Protein Length:367
Start: 2647394
Stop: 2646291
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05980.1|AE004688_2 (AE004688) probable periplasmic spermidine/putrescine-binding protein [Pseudomonas aeruginosa PAO1]
Identities = 367/367 (100%), Positives = 367/367 (100%)
Sequence:
ATGAAAAAAGTTTGTGCACTGGCGTTATCGATCCTGACGACGATCGGTGCGACAGCGGCGGACAGTGCATGG
GCTGCGCAAACCAGCGTCCATCTTTACAACTGGTATGACTTCATCGCCCCGGAAACGCCCAAGGCTTTCCAG
AAGGAAACCGGCACCCGTGTCGTCCTCGACACCTTCGACAGCGCCGAGACCGCGCAGGGCAAACTGATGGTC
GGCCGCTCCGGCTACGACGTGGTGGTGATCACCTCCAACATCCTGCCCGGACTGATCAAGGCAGGCGTCCTC
CAGGAACTCGACCGCGATCGGCTCCCCCACTGGAAGAACCTCGACGCGGACATCCTCGGGAAGCTTCAGGCC
AACGATCCCGGCAATCGCTATGCCGTACCTTATCTCTGGGGAACCACCGGGATCGCCTACGACGTGGACAAG
GTCCGCAAGCTGCTCGGCCCCGACGCGCCGGTCGACTCCTGGGACCTGGTCTTCAAGGAGGAGAATATCTCC
CGCCTCAGCCAGTGCGGCGTGGCCACGCTGGACTCCTCCACCGAGCTGGTTTCCATCGCCCTCAACTACCTG
GGCCTGCCGCACAACAGCCAGAATCCCGAGGATTACCAGAAAGCCCAGGAACTGTTGCTGAAGGTTCGCCCC
TACATTCGCTATTTCGACTCCTCCAGAGTCGACACCGACCTCTCCAACGGCAACGTCTGCGTGGTGGTCGGC
TGGCAGGGCACGGCCTACATGGCCCAGGTCAACAACGAACAGGCCGGGAACGGTCGCCATATCGCCTACAGC
ATTCCCCGGGAAGGCTCGCTGGTCTGGGCCGAGAACATGGTGCTGCTCAAGGATGCACCGCATCCGCAGCAA
GGTTATGCGCTGATCGACTACCTGCTGCGTCCGGAGGTCATCGCCAGGACCTCCAACTACGTGGGCTATCCG
AATGGCAACCAGGCGGCGCTGCCGCTGGTGGAGCGGAAGCTGCGGGAAAACCCGGCGGTTTACCTGAGCAAG
GAAACCATGGCGACCCTCTTCCCGCTGGAAACCCTGCCACTGAAGGTCGAGAGAATCCGTACCCGGGTCTGG
AGCCGAGTCAAGACCGGGAGCTGA
Translation:
MKKVCALALSILTTIGATAADSAWAAQTSVHLYNWYDFIAPETPKAFQKETGTRVVLDTFDSAETAQGKLMV
GRSGYDVVVITSNILPGLIKAGVLQELDRDRLPHWKNLDADILGKLQANDPGNRYAVPYLWGTTGIAYDVDK
VRKLLGPDAPVDSWDLVFKEENISRLSQCGVATLDSSTELVSIALNYLGLPHNSQNPEDYQKAQELLLKVRP
YIRYFDSSRVDTDLSNGNVCVVVGWQGTAYMAQVNNEQAGNGRHIAYSIPREGSLVWAENMVLLKDAPHPQQ
GYALIDYLLRPEVIARTSNYVGYPNGNQAALPLVERKLRENPAVYLSKETMATLFPLETLPLKVERIRTRVW
SRVKTGS*
AnnotationID:1724GeneID:1715
AnnotatorUID: diggins
Modification Date/Time: 2005-03-07 08:08:06
Gene Name:
Confidence Code:3
GeneProduct:putative periplasmic spermidine/putrescine-binding protein
Cell Localization:()
Synonyms:potF
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:putative ABC transporter, periplasmic polyamine-binding protein
Functional Category Confidence Code:5
COGs:COG0687
Secondary Functional Category(ies):amino acid biosynthesis and metabolims
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN69439.1|AE016579_10 (AE016788) ABC transporter, periplasmic polyamine-binding protein,
           putative [Pseudomonas putida KT2440]
          Length = 369

 Score =  489 bits (1260), Expect = e-138
 Identities = 231/360 (64%), Positives = 288/360 (80%)

>gb|AAC15511.1| (L49465) putative putrescine/spermidine binding protein
           [Pseudomonas fluorescens]
          Length = 377

 Score =  392 bits (1007), Expect = e-108
 Identities = 186/358 (51%), Positives = 258/358 (72%), Gaps = 5/358 (1%)

>gb|AAN54338.1|AE015571_1 (AE015571) polyamine ABC transporter, periplasmic polyamine-binding
           protein [Shewanella oneidensis MR-1]
          Length = 367

 Score =  349 bits (895), Expect = 2e-95
 Identities = 175/367 (47%), Positives = 247/367 (67%), Gaps = 2/367 (0%)






 
Structural Features:
COG0687, PotD, Spermidine/putrescine-binding periplasmic protein 
[Amino acid transport and metabolism]
CD-Length = 363 residues,  98.6% aligned
Score =  333 bits (856), Expect = 2e-92
Identities = 147/367 (40%), Positives = 212/367 (57%), Gaps = 10/367 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:3433




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20580




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33157




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1715_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:1715
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3013
                           PA2592_tr
                           translation of PA2592
                           367
                           99.99
                           99.99
                           0
                           1910.0
     

     
     
93672
                           gb|AAG05980.1|AE004688_2
                           (AE004688) probable periplasmic spermidine/putrescine-binding protein [Pseudomonas aeruginosa PAO1]
                           367
                           99.99
                           99.99
                           0
                           1910.0
     

     
     
93673
                           gb|AAN69439.1|AE016579_10
                           (AE016788) ABC transporter, periplasmic polyamine-binding protein, putative [Pseudomonas putida KT2440]
                           369
                           63.14
                           79.13
                           2
                           1262.0
     

     
     
3014
                           PA2711_tr
                           translation of PA2711
                           367
                           50.13
                           69.48
                           4
                           986.0
     

     
     
93675
                           gb|AAG06099.1|AE004699_4
                           (AE004699) probable periplasmic spermidine/putrescine-binding protein [Pseudomonas aeruginosa PAO1]
                           367
                           50.13
                           69.48
                           4
                           986.0
     

     






      

          
GC ORFID: 44717How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2592Glimmer Score: 

          
Start: 2306532Stop: 2307635

          
Length: 1104


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2592 translation of PA2592Homolog Bit Score 740.0

          
Other Homologs:  t_PA2711 (ORF: BLASTX 385.0), t_PA1410 (ORF: BLASTX 379.0), t_PA0301 (ORF: BLASTX 364.0), t_PA0300 (ORF: BLASTX 359.0), psyr_15may02_Scaffold6_revised_gene6511 (ORF: BLASTX 342.0), psyr_15may02_Scaffold6_revised_gene6512 (ORF: BLASTX 338.0), psyr_15may02_Scaffold7_revised_gene7441 (ORF: BLASTX 308.0), t_PA2592 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAAAAAGTTTGTGCACTGGCGTTATCGATCCTGACGACGATCGGTGCGACAGCGGCGGACAGTGCATGG 
GCTGCGCAAACCAGCGTCCATCTTTACAACTGGTATGACTTCATCGCCCCGGAAACGCCCAAGGCTTTCCAG 
AAGGAAACCGGCACCCGTGTCGTCCTCGACACCTTCGACAGCGCCGAGACCGCGCAGGGCAAACTGATGGTC 
GGCCGCTCCGGCTACGACGTGGTGGTGATCACCTCCAACATCCTGCCCGGACTGATCAAGGCAGGCGTCCTC 
CAGGAACTCGACCGCGATCGGCTCCCCCACTGGAAGAACCTCGACGCGGACATCCTCGGGAAGCTTCAGGCC 
AACGATCCCGGCAATCGCTATGCCGTACCTTATCTCTGGGGAACCACCGGGATCGCCTACGACGTGGACAAG 
GTCCGCAAGCTGCTCGGCCCCGACGCGCCGGTCGACTCCTGGGACCTGGTCTTCAAGGAGGAGAATATCTCC 
CGCCTCAGCCAGTGCGGCGTGGCCACGCTGGACTCCTCCACCGAGCTGGTTTCCATCGCCCTCAACTACCTG 
GGCCTGCCGCACAACAGCCAGAATCCCGAGGATTACCAGAAAGCCCAGGAACTGTTGCTGAAGGTTCGCCCC 
TACATTCGCTATTTCGACTCCTCCAGAGTCGACACCGACCTCTCCAACGGCAACGTCTGCGTGGTGGTCGGC 
TGGCAGGGCACGGCCTACATGGCCCAGGTCAACAACGAACAGGCCGGGAACGGTCGCCATATCGCCTACAGC 
ATTCCCCGGGAAGGCTCGCTGGTCTGGGCCGAGAACATGGTGCTGCTCAAGGATGCACCGCATCCGCAGCAA 
GGTTATGCGCTGATCGACTACCTGCTGCGTCCGGAGGTCATCGCCAGGACCTCCAACTACGTGGGCTATCCG 
AATGGCAACCAGGCGGCGCTGCCGCTGGTGGAGCGGAAGCTGCGGGAAAACCCGGCGGTTTACCTGAGCAAG 
GAAACCATGGCGACCCTCTTCCCGCTGGAAACCCTGCCACTGAAGGTCGAGAGAATCCGTACCCGGGTCTGG 
AGCCGAGTCAAGACCGGGAGCTGA