ViewGene
Locus Name:
GeneID:
Location:

GeneID:1562
TrivialName: PA14_19430
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_19430
PAO1 Ortholog Locus:PA3454
Sequence Length:1185
Protein Length:394
Start: 1680525
Stop: 1681709
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06842.1|AE004766_3 (AE004766) probable acyl-CoA thiolase [Pseudomonas aeruginosa PAO1]
Identities = 393/394 (99%), Positives = 393/394 (99%)
Sequence:
ATGCGTGAAGTGGTGATCGTCGACAGCGTCCGGACCGGCCTGGCCAAGTCCTTCCGCGGCAAGTTCAACCTG
ACCCGGCCGGACGACATGGCCGCCCACTGTGTCGACGCGCTGCTGGCGCGCAACGACCTCGACCCGCTGCTG
GTGGATGACTGCATCGTCGGCGCCGGCTCCAACGAAGGCGCCCAGGGCCACAACATCGGGCGCAACGTGGCG
GTGCTCTCCGGCCTCGGCATCCAGGTGCCGGGGATGACCCTCAACCGCTACTGTTCCTCCGGCCTGCAGGCG
ATCGCCATCGCCGCCAACCAGATCGCTTCCGGTTGCAGCGAGGTGATCGTCGCCGGCGGCGTCGAGTCGATC
ACCCTGACCCTGAAGAGCGTCAACACCGACCACCTGGTGAACCCGCTGCTGCAAAGGGAGGTGCCGGGCATC
TACTACCCCATGGGGCAGACCGCCGAGATCGTCGCCCGTCGCTACGGCATCACCCGCGAGGCCCAGGATGCC
TACGCCCTGCAGAGCCAGCAGCGGATGGCGCGAGCCCAGGCGGACGGGCTGTTCGCCGACGAGATCGTGCCG
ATGACCACCCGCTACGCGGTGGAGGACAAGGCCAGCGGCGAGAAGCAGGTGCTCGACGGAGTGGTCGACCGC
GACGACTGCAACCGCCCGGACACCACCCTCGAAGGCCTGGCCTCGCTGAAGCCGGCGTTCGCCGAGGACGGT
TCGGTCACCGCCGGCAACGCCTCGCAACTCTCCGACGGCGCCTCGATGACCCTGCTGATGAGCCTGGAGAAA
GCGCTGGCGCTGGGCCTGGAGCCGAAGGCCTTCTTCCGTGGCTTCACCGTGGCCGGCTGCGAGCCGGACGAA
ATGGGCATCGGTCCGGTGTTCTCGGTGCCGAAGCTGCTCAAGGCGAAGGGGCTGAAGATCGCCGACGTCGAT
CTCTGGGAACTCAACGAAGCCTTCGCCTCGCAGTGCCTGTACTGCCGCGACCGGCTGGAGATCGACAACGAG
AAGTACAACGTCAATGGCGGCTCCATCGCCATCGGCCACCCGTTCGGCATGACCGGCTCGCGCCAGGTCGGT
CATCTGGTCCGCGAACTGCGCCGGCGCAACCTGCGCTACGGCGTGGTGACCATGTGCGTCGGCGGCGGCATG
GGCGCCAGCGGGCTGTTCGAAGCCGTACGCTGA
Translation:
MREVVIVDSVRTGLAKSFRGKFNLTRPDDMAAHCVDALLARNDLDPLLVDDCIVGAGSNEGAQGHNIGRNVA
VLSGLGIQVPGMTLNRYCSSGLQAIAIAANQIASGCSEVIVAGGVESITLTLKSVNTDHLVNPLLQREVPGI
YYPMGQTAEIVARRYGITREAQDAYALQSQQRMARAQADGLFADEIVPMTTRYAVEDKASGEKQVLDGVVDR
DDCNRPDTTLEGLASLKPAFAEDGSVTAGNASQLSDGASMTLLMSLEKALALGLEPKAFFRGFTVAGCEPDE
MGIGPVFSVPKLLKAKGLKIADVDLWELNEAFASQCLYCRDRLEIDNEKYNVNGGSIAIGHPFGMTGSRQVG
HLVRELRRRNLRYGVVTMCVGGGMGASGLFEAVR*
AnnotationID:1571GeneID:1562
AnnotatorUID: diggins
Modification Date/Time: 2005-08-21 08:08:53
Gene Name:
Confidence Code:3
GeneProduct:putative acyl-CoA thiolase
Cell Localization:()
Synonyms:PA3454
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(13) Fatty acid and phospholipid metabolism
Alternate Gene Product Name:putative acetyl-CoA acetyltransferase
Functional Category Confidence Code:5
COGs:pfam00108,pfam02803,COG0183
Secondary Functional Category(ies):central intermediary metabolism
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAF10997.1|AE001987_5 (AE001987) acetyl-CoA acetyltransferase [Deinococcus radiodurans]
          Length = 402

 Score =  425 bits (1093), Expect = e-118
 Identities = 221/394 (56%), Positives = 277/394 (70%), Gaps = 2/394 (0%)

>emb|CAE31165.1| (BX640439) probable thiolase [Bordetella bronchiseptica]
          Length = 394

 Score =  400 bits (1029), Expect = e-111
 Identities = 205/394 (52%), Positives = 270/394 (68%)

>dbj|BAC69094.1| (AP005026) putative acyl-CoA thiolase [Streptomyces avermitilis
           MA-4680]
          Length = 399

 Score =  395 bits (1015), Expect = e-109
 Identities = 213/399 (53%), Positives = 271/399 (67%), Gaps = 6/399 (1%)








 
Structural Features:
pfam00108, Thiolase_N, Thiolase, N-terminal domain.
CD-Length = 264 residues,  99.2% aligned
Score =  216 bits (552), Expect = 3e-57
Identities = 114/273 (41%), Positives = 153/273 (56%), Gaps = 23/273 (8%)

pfam02803, Thiolase_C, Thiolase, C-terminal domain.
CD-Length = 123 residues, 100.0% aligned
Score =  159 bits (405), Expect = 4e-40
Identities = 60/123 (48%), Positives = 85/123 (69%)

COG0183, PaaJ, Acetyl-CoA acetyltransferase [Lipid metabolism]
CD-Length = 392 residues, 100.0% aligned
Score =  328 bits (841), Expect = 9e-91
Identities = 162/410 (39%), Positives = 216/410 (52%), Gaps = 35/410 (8%)







   
Genomic Context:






   
Comment:


















    ReferenceID:3127




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20274




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32116




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1562_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:1562
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2772
                           PA3454_tr
                           translation of PA3454
                           394
                           99.74
                           99.74
                           0
                           2005.0
     

     
     
86997
                           gb|AAG06842.1|AE004766_3
                           (AE004766) probable acyl-CoA thiolase [Pseudomonas aeruginosa PAO1]
                           394
                           99.74
                           99.74
                           0
                           2005.0
     

     
     
86998
                           gb|AAK25424.1|
                           (AE006005) thiolase family protein [Caulobacter crescentus CB15]
                           396
                           57.82
                           71.46
                           3
                           1148.0
     

     
     
87000
                           dbj|BAC52665.1|
                           (AP005962) acetyl-CoA acetyltransferase [Bradyrhizobium japonicum USDA 110]
                           398
                           56.28
                           69.59
                           12
                           1074.0
     

     
     
86999
                           gb|AAF10997.1|AE001987_5
                           (AE001987) acetyl-CoA acetyltransferase [Deinococcus radiodurans] [Deinococcus radiodurans R1]
                           404
                           54.70
                           68.81
                           12
                           1097.0
     

     






      

          
GC ORFID: 44564How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3454Glimmer Score: 

          
Start: 3273407Stop: 3272223

          
Length: 1185


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3454 translation of PA3454Homolog Bit Score 776.0

          
Other Homologs:  t_PA0228 (ORF: BLASTX 271.0), psyr_15may02_Scaffold9_revised_gene8045 (ORF: BLASTX 271.0), t_PA2940 (ORF: BLASTX 270.0), psyr_15may02_Scaffold15_revised_gene2524 (ORF: BLASTX 261.0), t_PA2001 (ORF: BLASTX 258.0), t_PA3013 (ORF: BLASTX 256.0), psyr_15may02_Scaffold5_revised_gene6193 (ORF: BLASTX 248.0), t_PA3454 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCGTGAAGTGGTGATCGTCGACAGCGTCCGGACCGGCCTGGCCAAGTCCTTCCGCGGCAAGTTCAACCTG 
ACCCGGCCGGACGACATGGCCGCCCACTGTGTCGACGCGCTGCTGGCGCGCAACGACCTCGACCCGCTGCTG 
GTGGATGACTGCATCGTCGGCGCCGGCTCCAACGAAGGCGCCCAGGGCCACAACATCGGGCGCAACGTGGCG 
GTGCTCTCCGGCCTCGGCATCCAGGTGCCGGGGATGACCCTCAACCGCTACTGTTCCTCCGGCCTGCAGGCG 
ATCGCCATCGCCGCCAACCAGATCGCTTCCGGTTGCAGCGAGGTGATCGTCGCCGGCGGCGTCGAGTCGATC 
ACCCTGACCCTGAAGAGCGTCAACACCGACCACCTGGTGAACCCGCTGCTGCAAAGGGAGGTGCCGGGCATC 
TACTACCCCATGGGGCAGACCGCCGAGATCGTCGCCCGTCGCTACGGCATCACCCGCGAGGCCCAGGATGCC 
TACGCCCTGCAGAGCCAGCAGCGGATGGCGCGAGCCCAGGCGGACGGGCTGTTCGCCGACGAGATCGTGCCG 
ATGACCACCCGCTACGCGGTGGAGGACAAGGCCAGCGGCGAGAAGCAGGTGCTCGACGGAGTGGTCGACCGC 
GACGACTGCAACCGCCCGGACACCACCCTCGAAGGCCTGGCCTCGCTGAAGCCGGCGTTCGCCGAGGACGGT 
TCGGTCACCGCCGGCAACGCCTCGCAACTCTCCGACGGCGCCTCGATGACCCTGCTGATGAGCCTGGAGAAA 
GCGCTGGCGCTGGGCCTGGAGCCGAAGGCCTTCTTCCGTGGCTTCACCGTGGCCGGCTGCGAGCCGGACGAA 
ATGGGCATCGGTCCGGTGTTCTCGGTGCCGAAGCTGCTCAAGGCGAAGGGGCTGAAGATCGCCGACGTCGAT 
CTCTGGGAACTCAACGAAGCCTTCGCCTCGCAGTGCCTGTACTGCCGCGACCGGCTGGAGATCGACAACGAG 
AAGTACAACGTCAATGGCGGCTCCATCGCCATCGGCCACCCGTTCGGCATGACCGGCTCGCGCCAGGTCGGT 
CATCTGGTCCGCGAACTGCGCCGGCGCAACCTGCGCTACGGCGTGGTGACCATGTGCGTCGGCGGCGGCATG 
GGCGCCAGCGGGCTGTTCGAAGCCGTACGCTGA