ViewGene
Locus Name:
GeneID:
Location:

GeneID:1396
TrivialName: PA14_31970
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_31970
PAO1 Ortholog Locus:PA2522
Sequence Length:1287
Protein Length:428
Start: 2784041
Stop: 2785327
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05910.1|AE004679_4 (AE004679) outer membrane protein precursor CzcC [Pseudomonas aeruginosa PAO1]
Identities = 427/428 (99%), Positives = 428/428 (100%)
Sequence:
ATGCCGATACTTCGGCCACTGGCCAGCGCGGGGAAACGCGCCTGCTGGCTACTGATGGGACTCTGCCTGGGC
CTGCCGGCCCTGGCGAACGAGGCGCCGGTGAGTTTCAACGGCACTTCGATCAGCCTCGAGCAGGCGTTGGAG
CGGGCCCTGCGGAGCAACCCCGAGCTGGCGGCGGTGGGGCGCGAGACCGAGATCGCCAGCGGCGCGCGGCAG
CAGGCCGGGCTGATCCCCAACCCGGATCTGTCCTGGAGCGTCGAGGACACCCGCCAGGGCAATCGCCAGACC
AGCGTCAGCATCGCCCAGCCGCTGGAGCTGGGCGGCAAGCGCGGCGCGCGGGTGGAGGTGGCGAAACGCGGC
AGCGAGATCGCCTGGACCCAACTGGAAGTCCGTCGCGCCGAACTGCGCGCCCAGGTCCGTGGCGCCTACTAC
GCGGCGCTGACCGCGCAGGAGCGGGTGCGCCTGGCGAAGACCTCCCTGGACCTGGCCAGGCGTGCGCTGCAG
GCCGCCGACCGGCGGGTCAAGGCCGGCAGCATCTCTTCGGTGGAACGGGTCCGCGCCCAGGTCCTGGCGGAC
AACGCCCAGCTCGACCTGAGCCAGGCGGAGCTGGAGCAGCAGCGCACCTATGTGCAGCTGTCGAGCACCTGG
GACGAGCCGCAGCCAGGCTTCGCCAAGGTCGGCGGCGCGCTCGACGCGGTGCCGGCGAGCATCACCCGCGGC
GCCTTGCTGCGCCATCTCGACGAGTCGCCGACCTTGCGCCTGGCGGCCCAGGAGGTAGCGCGCGGCGAGGCC
CAGGTCGACCTGGAGAAGCGCCAGCGCATCCCCAACCTGACGGTCAGCATCGGCAGCAAGTACGACCAGACC
GCCCGCGACGGACGTGGCGAACGGGTCAACCTGATCGGCCTGTCGATGCCGCTGCCGCTGTTCGACCGCAAC
CAGGGCAACATCTACGCCGCCCAGAGCCGCGCCGACCAGGCCCGCGACCTGCAGCGCGCGACCCTGCTGCGC
CTGCGCAGCGAAGCGGTACAGGCCTACGACCAGTTGCGCACCTCGGAACAGGAGCTGGCGCTGGTCCGTCGC
GACCTGCTGCCCGGCGCACAGAGCGCGCTGGACTCGATGACCCGCGGCTTCGAGATGGGCAAGTTCAACTTC
CTCGACGTGCTCGACGCCCAGCGCACCCTGGTCGGCGTCCGTGCCCAGTACGTGCGCGCGCTGGACGCCGCG
GCGCAGGCGCGGGTGAGCATGGAGCGCCTGCTCGGCGAAGACATCGGCCACCTCGGCCAGTGA
Translation:
MPILRPLASAGKRACWLLMGLCLGLPALANEAPVSFNGTSISLEQALERALRSNPELAAVGRETEIASGARQ
QAGLIPNPDLSWSVEDTRQGNRQTSVSIAQPLELGGKRGARVEVAKRGSEIAWTQLEVRRAELRAQVRGAYY
AALTAQERVRLAKTSLDLARRALQAADRRVKAGSISSVERVRAQVLADNAQLDLSQAELEQQRTYVQLSSTW
DEPQPGFAKVGGALDAVPASITRGALLRHLDESPTLRLAAQEVARGEAQVDLEKRQRIPNLTVSIGSKYDQT
ARDGRGERVNLIGLSMPLPLFDRNQGNIYAAQSRADQARDLQRATLLRLRSEAVQAYDQLRTSEQELALVRR
DLLPGAQSALDSMTRGFEMGKFNFLDVLDAQRTLVGVRAQYVRALDAAAQARVSMERLLGEDIGHLGQ*
AnnotationID:1405GeneID:1396
AnnotatorUID: diggins
Modification Date/Time: 2005-03-06 18:06:19
Gene Name:czcC
Confidence Code:2
GeneProduct:cobalt/zinc/cadmium efflux RND transporter, outer membrane protein, CzcC family
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1538,pfam02321
Secondary Functional Category(ies):membrane proteins
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAG09627.1| (AY007258) CztC [Pseudomonas fluorescens]
          Length = 406

 Score =  348 bits (893), Expect = 4e-95
 Identities = 191/382 (50%), Positives = 252/382 (65%), Gaps = 4/382 (1%)

>gb|AAN65679.1|AE016194_9 (AE016774) cobalt/zinc/cadmium efflux RND transporter, outer
           membrane protein, CzcC family [Pseudomonas putida
           KT2440]
          Length = 423

 Score =  341 bits (875), Expect = 4e-93
 Identities = 198/406 (48%), Positives = 259/406 (63%), Gaps = 7/406 (1%)

>gb|AAO53921.1| (AE016857) metal ion efflux outer membrane protein, putative
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 408

 Score =  332 bits (852), Expect = 2e-90
 Identities = 189/390 (48%), Positives = 252/390 (64%), Gaps = 4/390 (1%)








 
Structural Features:
COG1538, TolC, Outer membrane protein 
[Cell envelope biogenesis, outer membrane / Intracellular trafficking and secretion]
CD-Length = 457 residues,  87.5% aligned
Score =  145 bits (367), Expect = 9e-36
Identities = 102/401 (25%), Positives = 170/401 (42%), Gaps = 23/401 (5%)

pfam02321, OEP, Outer membrane efflux protein.
CD-Length = 188 residues,  95.7% aligned
Score = 73.9 bits (181), Expect = 4e-14
Identities = 55/187 (29%), Positives = 93/187 (49%), Gaps = 13/187 (6%)






   
Genomic Context:






   
Comment:


















    ReferenceID:2795




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19940




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33084




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1396_cdd.html


    		




 
URL:

















    ReferenceID:34525




  
Author/Investigator(s):
        Grosse C, Grass G, Anton A, Franke S, Santos AN, Lawley B, Brown NL, Nies DH.





  
Title:
        Transcriptional organization of the czc heavy-metal homeostasis determinant from Alcaligenes eutrophus.




  
PubMed:
        10198000


MedLine:
        




    
Source:
J Bacteriol. 1999 Apr;181(8):2385-93.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10198000









           
Homologs By Global Alignment 

           

       



     
Gene ID:1396
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2544
                           PA2522_tr
                           translation of PA2522
                           428
                           99.76
                           99.99
                           0
                           2105.0
     

     
     
80217
                           gb|AAG05910.1|AE004679_4
                           (AE004679) outer membrane protein precursor CzcC [Pseudomonas aeruginosa PAO1]
                           428
                           99.76
                           99.99
                           0
                           2105.0
     

     
     
80218
                           emb|CAB56469.1|
                           (Y14018) czrC protein [Pseudomonas aeruginosa]
                           431
                           87.70
                           89.09
                           8
                           1777.0
     

     
     
80219
                           gb|AAG09627.1|
                           (AY007258) CztC [Pseudomonas fluorescens]
                           429
                           47.08
                           62.00
                           24
                           893.0
     

     
     
80221
                           gb|AAN68020.1|AE016434_11
                           (AE016783) cobalt-zinc-cadmium resistance protein CzcC, putative [Pseudomonas putida KT2440]
                           446
                           46.41
                           59.19
                           39
                           878.0
     

     






      

          
GC ORFID: 44398How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2522Glimmer Score: 

          
Start: 2169885Stop: 2168599

          
Length: 1287


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2522 translation of PA2522Homolog Bit Score 815.0

          
Other Homologs:  psyr_15may02_Scaffold6_revised_gene6875 (ORF: BLASTX 336.0), t_PA2522 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCCGATACTTCGGCCACTGGCCAGCGCGGGGAAACGCGCCTGCTGGCTACTGATGGGACTCTGCCTGGGC 
CTGCCGGCCCTGGCGAACGAGGCGCCGGTGAGTTTCAACGGCACTTCGATCAGCCTCGAGCAGGCGTTGGAG 
CGGGCCCTGCGGAGCAACCCCGAGCTGGCGGCGGTGGGGCGCGAGACCGAGATCGCCAGCGGCGCGCGGCAG 
CAGGCCGGGCTGATCCCCAACCCGGATCTGTCCTGGAGCGTCGAGGACACCCGCCAGGGCAATCGCCAGACC 
AGCGTCAGCATCGCCCAGCCGCTGGAGCTGGGCGGCAAGCGCGGCGCGCGGGTGGAGGTGGCGAAACGCGGC 
AGCGAGATCGCCTGGACCCAACTGGAAGTCCGTCGCGCCGAACTGCGCGCCCAGGTCCGTGGCGCCTACTAC 
GCGGCGCTGACCGCGCAGGAGCGGGTGCGCCTGGCGAAGACCTCCCTGGACCTGGCCAGGCGTGCGCTGCAG 
GCCGCCGACCGGCGGGTCAAGGCCGGCAGCATCTCTTCGGTGGAACGGGTCCGCGCCCAGGTCCTGGCGGAC 
AACGCCCAGCTCGACCTGAGCCAGGCGGAGCTGGAGCAGCAGCGCACCTATGTGCAGCTGTCGAGCACCTGG 
GACGAGCCGCAGCCAGGCTTCGCCAAGGTCGGCGGCGCGCTCGACGCGGTGCCGGCGAGCATCACCCGCGGC 
GCCTTGCTGCGCCATCTCGACGAGTCGCCGACCTTGCGCCTGGCGGCCCAGGAGGTAGCGCGCGGCGAGGCC 
CAGGTCGACCTGGAGAAGCGCCAGCGCATCCCCAACCTGACGGTCAGCATCGGCAGCAAGTACGACCAGACC 
GCCCGCGACGGACGTGGCGAACGGGTCAACCTGATCGGCCTGTCGATGCCGCTGCCGCTGTTCGACCGCAAC 
CAGGGCAACATCTACGCCGCCCAGAGCCGCGCCGACCAGGCCCGCGACCTGCAGCGCGCGACCCTGCTGCGC 
CTGCGCAGCGAAGCGGTACAGGCCTACGACCAGTTGCGCACCTCGGAACAGGAGCTGGCGCTGGTCCGTCGC 
GACCTGCTGCCCGGCGCACAGAGCGCGCTGGACTCGATGACCCGCGGCTTCGAGATGGGCAAGTTCAACTTC 
CTCGACGTGCTCGACGCCCAGCGCACCCTGGTCGGCGTCCGTGCCCAGTACGTGCGCGCGCTGGACGCCGCG 
GCGCAGGCGCGGGTGAGCATGGAGCGCCTGCTCGGCGAAGACATCGGCCACCTCGGCCAGTGA