ViewGene
Locus Name:
GeneID:
Location:

GeneID:1389
TrivialName: PA14_35110
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_35110
PAO1 Ortholog Locus:PA2278
Sequence Length:1284
Protein Length:427
Start: 3125970
Stop: 3124687
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05666.1|AE004653_9 (AE004653) ArsB protein [Pseudomonas aeruginosa PAO1]
Identities = 425/427 (99%), Positives = 426/427 (99%)
Sequence:
ATGCTGATCGCCTTCGCCATCTTCCTGTTCACCCTGGTCCTGGTCATCTGGCAGCCCAGGGGCCTCGGCATC
GGCTGGAGCGCCAGCATCGGCGCCCTGCTCGCCCTGGCGCTGGGCAGCGTCGCGCCGGGCGACATCCCGACC
GTCTGGAACATCGTCTGGAATGCGACCGCCACCTTCATCGCGGTGATCGTCATCAGCCTGCTGCTGGACGAG
GCCGGCTTCTTCGAATGGGCCGCGCTGCACGTCGCGCGCTGGGCCGGCGGCGACGGCCGGCGCCTGTTCGCC
TTCTGCGTGCTGCTCGGCGCGGCGGTCTCGGCGCTGTTCGCCAACGACGGCGCGGCGCTGATCCTCACGCCC
ATCGTGATGTCGATGCTGCTGGCCCTGCGCTTCAGCCCGGCGGCGACCCTGGCCTTCGTCATGGCGGCGGGC
TTCATCGCCGACAGCGCCAGCCTGCCGCTGGTGGTCTCGAACCTGGTGAACATCGTCTCGGCCGACTACTTC
GGGCTCGGCTTCGGCGAGTACGCCGCGGTGATGTTGCCGGTAAACCTGGTGAGCGTCGCGACCTCCCTGCTG
GTGCTGTTCCTCTATTTCCGCCGCGACCTGCCGCCGGTCTATGCGCTGGAGCAATTGAAGCCGCCGCGGGCG
GCGATCCGCGACCGCGCGACCTTCGTCGTCGGTGGCTGGATGCTGCTGGTCCTGCTCGCCGGGCTGTTCGCC
CTGGAGCCGCTGGGCGTCCCCGTCAGCGCGGTGGCCGCGACCTGTGCGGCGCTGCTGCTGGCGGTGGCCGGG
CGCGGGCGGGTGATCTCGACCCGGCGGGTGCTGCGCGAAGCGCCCTGGCAGATCGTGGTCTTCTCGCTGGGC
ATGTACCTGGTGGTCTACGGCCTGAAGAACGCCGGTCTCACCGACCTCCTGGCGCAGCTCCTGAACCGCCTG
GCCGAGCACGGCGTATGGGCGGCCGCGCTGGGTACCGGACTGCTTTCCGCCGCCCTCTCGTCGGTAATGAAC
AACATGCCCAGCGTGCTGCTCGGCGCCCTGTCGATCCAGGCCAGCGACACCGCCGGCGCGGTGCGCGAAGCG
ATGATCTACGCCAACGTCATCGGCTGCGACCTGGGGCCGAAGATCACCCCTATCGGCAGCCTGGCGACGCTG
CTCTGGCTGCACGTGCTGGCGCGCAAAGGCATGCGCATCACCTGGGGCTACTACTTCAGGGTCGGCGCCCTG
CTGACCCTGCCGGTCCTGCTGGCGACCCTTTCGGCCCTGGCCCTGCGCCTGGCCATCTGA
Translation:
MLIAFAIFLFTLVLVIWQPRGLGIGWSASIGALLALALGSVAPGDIPTVWNIVWNATATFIAVIVISLLLDE
AGFFEWAALHVARWAGGDGRRLFAFCVLLGAAVSALFANDGAALILTPIVMSMLLALRFSPAATLAFVMAAG
FIADSASLPLVVSNLVNIVSADYFGLGFGEYAAVMLPVNLVSVATSLLVLFLYFRRDLPPVYALEQLKPPRA
AIRDRATFVVGGWMLLVLLAGLFALEPLGVPVSAVAATCAALLLAVAGRGRVISTRRVLREAPWQIVVFSLG
MYLVVYGLKNAGLTDLLAQLLNRLAEHGVWAAALGTGLLSAALSSVMNNMPSVLLGALSIQASDTAGAVREA
MIYANVIGCDLGPKITPIGSLATLLWLHVLARKGMRITWGYYFRVGALLTLPVLLATLSALALRLAI*
AnnotationID:1398GeneID:1389
AnnotatorUID: diggins
Modification Date/Time: 2005-03-04 09:09:49
Gene Name:arsB
Confidence Code:1
GeneProduct:arsenical pump membrane protein
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:arsenite efflux transporter
Functional Category Confidence Code:5
COGs:pfam02040,COG1055
Secondary Functional Category(ies):membrane protein
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN68325.1|AE016466_10 (AE016784) arsenite efflux transporter [Pseudomonas putida KT2440]
          Length = 427

 Score =  698 bits (1802), Expect = 0.0
 Identities = 352/427 (82%), Positives = 388/427 (90%)

>emb|CAB88405.1| (AJ288983) ArsB transmembrane efflux channel [Serratia marcescens]
          Length = 429

 Score =  610 bits (1572), Expect = e-174
 Identities = 311/426 (73%), Positives = 360/426 (84%)

>gb|AAP19195.1| (AE016992) arsenical pump membrane protein [Shigella flexneri 2a
           str. 2457T]
          Length = 429

 Score =  603 bits (1556), Expect = e-172
 Identities = 300/426 (70%), Positives = 360/426 (84%)






 
Structural Features:
pfam02040, ArsB, Arsenical pump membrane protein.
CD-Length = 423 residues, 100.0% aligned
Score =  594 bits (1534), Expect = 4e-171
Identities = 300/423 (70%), Positives = 358/423 (84%)

COG1055, ArsB, Na+/H+ antiporter NhaD and related arsenite permeases 
[Inorganic ion transport and metabolism]
CD-Length = 424 residues,  99.5% aligned
Score =  322 bits (827), Expect = 4e-89
Identities = 185/425 (43%), Positives = 263/425 (61%), Gaps = 4/425 (0%)






   
Genomic Context:






   
Comment:


















    ReferenceID:2781




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19926




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32503




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1389_cdd.html


    		




 
URL:

















    ReferenceID:34455




  
Author/Investigator(s):
        Cai J, Salmon K, DuBow MS.





  
Title:
        A chromosomal ars operon homologue of Pseudomonas aeruginosa confers increased resistance to arsenic and antimony in Escherichia coli.




  
PubMed:
        9802012


MedLine:
        




    
Source:
Microbiology. 1998 Oct;144 ( Pt 10):2705-13.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9802012

















    ReferenceID:34456




  
Author/Investigator(s):
        Diorio C, Cai J, Marmor J, Shinder R, DuBow MS.





  
Title:
        An Escherichia coli chromosomal ars operon homolog is functional in arsenic detoxification and is conserved in gram-negative bacteria.




  
PubMed:
        7721697


MedLine:
        




    
Source:
J Bacteriol. 1995 Apr;177(8):2050-6.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=7721697









           
Homologs By Global Alignment 

           

       



     
Gene ID:1389
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2537
                           PA2278_tr
                           translation of PA2278
                           427
                           99.53
                           99.76
                           0
                           2108.0
     

     
     
80068
                           gb|AAG05666.1|AE004653_9
                           (AE004653) ArsB protein [Pseudomonas aeruginosa PAO1]
                           427
                           99.53
                           99.76
                           0
                           2108.0
     

     
     
80069
                           gb|AAC69643.1|
                           (AF010234) ArsB [Pseudomonas aeruginosa]
                           428
                           96.96
                           97.42
                           4
                           2022.0
     

     
     
80070
                           gb|AAN68325.1|AE016466_10
                           (AE016784) arsenite efflux transporter [Pseudomonas putida KT2440]
                           427
                           82.43
                           90.86
                           0
                           1802.0
     

     
     
80071
                           gb|AAN67546.1|AE016383_8
                           (AE016781) arsenite efflux transporter [Pseudomonas putida KT2440]
                           427
                           75.40
                           88.05
                           0
                           1686.0
     

     






      

          
GC ORFID: 44391How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2278Glimmer Score: 

          
Start: 1827946Stop: 1829229

          
Length: 1284


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2278 translation of PA2278Homolog Bit Score 816.0

          
Other Homologs:  psyr_15may02_Scaffold10_revised_gene1595 (ORF: BLASTX 655.0), psyr_15may02_Scaffold1_revised_gene716 (ORF: BLASTX 610.0), t_PA2278 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCTGATCGCCTTCGCCATCTTCCTGTTCACCCTGGTCCTGGTCATCTGGCAGCCCAGGGGCCTCGGCATC 
GGCTGGAGCGCCAGCATCGGCGCCCTGCTCGCCCTGGCGCTGGGCAGCGTCGCGCCGGGCGACATCCCGACC 
GTCTGGAACATCGTCTGGAATGCGACCGCCACCTTCATCGCGGTGATCGTCATCAGCCTGCTGCTGGACGAG 
GCCGGCTTCTTCGAATGGGCCGCGCTGCACGTCGCGCGCTGGGCCGGCGGCGACGGCCGGCGCCTGTTCGCC 
TTCTGCGTGCTGCTCGGCGCGGCGGTCTCGGCGCTGTTCGCCAACGACGGCGCGGCGCTGATCCTCACGCCC 
ATCGTGATGTCGATGCTGCTGGCCCTGCGCTTCAGCCCGGCGGCGACCCTGGCCTTCGTCATGGCGGCGGGC 
TTCATCGCCGACAGCGCCAGCCTGCCGCTGGTGGTCTCGAACCTGGTGAACATCGTCTCGGCCGACTACTTC 
GGGCTCGGCTTCGGCGAGTACGCCGCGGTGATGTTGCCGGTAAACCTGGTGAGCGTCGCGACCTCCCTGCTG 
GTGCTGTTCCTCTATTTCCGCCGCGACCTGCCGCCGGTCTATGCGCTGGAGCAATTGAAGCCGCCGCGGGCG 
GCGATCCGCGACCGCGCGACCTTCGTCGTCGGTGGCTGGATGCTGCTGGTCCTGCTCGCCGGGCTGTTCGCC 
CTGGAGCCGCTGGGCGTCCCCGTCAGCGCGGTGGCCGCGACCTGTGCGGCGCTGCTGCTGGCGGTGGCCGGG 
CGCGGGCGGGTGATCTCGACCCGGCGGGTGCTGCGCGAAGCGCCCTGGCAGATCGTGGTCTTCTCGCTGGGC 
ATGTACCTGGTGGTCTACGGCCTGAAGAACGCCGGTCTCACCGACCTCCTGGCGCAGCTCCTGAACCGCCTG 
GCCGAGCACGGCGTATGGGCGGCCGCGCTGGGTACCGGACTGCTTTCCGCCGCCCTCTCGTCGGTAATGAAC 
AACATGCCCAGCGTGCTGCTCGGCGCCCTGTCGATCCAGGCCAGCGACACCGCCGGCGCGGTGCGCGAAGCG 
ATGATCTACGCCAACGTCATCGGCTGCGACCTGGGGCCGAAGATCACCCCTATCGGCAGCCTGGCGACGCTG 
CTCTGGCTGCACGTGCTGGCGCGCAAAGGCATGCGCATCACCTGGGGCTACTACTTCAGGGTCGGCGCCCTG 
CTGACCCTGCCGGTCCTGCTGGCGACCCTTTCGGCCCTGGCCCTGCGCCTGGCCATCTGA