ViewGene
Locus Name:
GeneID:
Location:

GeneID:1375
TrivialName: PA14_31870
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_31870
PAO1 Ortholog Locus:PA2528
Sequence Length:1281
Protein Length:426
Start: 2772055
Stop: 2773335
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05916.1|AE004680_4 (AE004680) probable RND efflux membrane fusion protein precursor [Pseudomonas aeruginosa PAO1]
Identities = 425/426 (99%), Positives = 425/426 (99%)
Sequence:
ATGACTCCAACGACCGGTAAATCCAAGTTCCGTACCCTGCGCCCGTGGCTGATCACCGCCCTGGCCTTCGCC
GCCGTGATCGGCCTGGTGATGTGGCTGGCGGCGCCCGCCTCGGCACCGTCCTCCGACGGGCGACCCGGTCGC
GGCGGCAAGCCGGGCGCCGCGCTGCCCAAGGCCAACGCGCTCACCGTCGGCGTGGCCAGGGTGGAGCAGGGC
GACCTGGCGCTGCATTTCAACGCGCTTGGCACCGTCACCGCCTTCAACACGGTGAACGTCAAGCCGCGGGTC
AACGGCGAGCTGGTCAAGGTGCTGTTCCAGGAGGGGCAGGAGGTCAAGGCCGGCGACCTGCTGGCGGTGGTC
GACCCGCGTACCTACAAGGCGGCGCTGGCCCAGGCCGAGGGCACGCTGATGCAGAACCAGGCGCAACTGAAG
AACGCCGAGATCGACCTGCAGCGCTACAAGGGGCTGTACGCCGAGGACTCGATCGCCAAGCAGACCCTGGAT
ACCCAGGAAGCCCAGGTCCGCCAGTTGCAGGGCACCATCCGTACCAACCAGGGCCAGGTCGACGACGCCCGC
CTCAACCTGACCTTCACCGAGGTCCGCGCACCGATTTCCGGGCGCCTCGGCCTGCGCCAGGTGGACATCGGC
AACCTGGTCACCAGCGGCGATACCACGCCGCTGGTGGTGATCACCCAGGTCAAGCCGATCTCGGTGGTGTTC
AGCCTGCCGCAGCAGCAGATCGGCACCGTCGTCGAGCAGATGAACGGCCCCGGCAAGCTGGCGGTCACCGCG
CTGGACCGCAACCAGGACAAGGTTCTCGCCGAAGGCACCCTGACCACCCTGGACAACCAGATCGACACCACC
ACCGGCACGGTCAAGCTCAAGGCACGCTTCGAGAACGCCGACGGCAAGCTGTTCCCCAACCAGTTCGTCAAC
GTGCGCCTGCTGGCGCAGACCCTCAAGGGCGTGCTGACCATTCCGGCCAACGCCGTGCAGCGCGGCACCAAC
GGTATCTATGTGTACGTGGTCGGCGCCGACAACAAGGTCAGCCAGCGCAGCGTCGCCATCGGCACCAGCGAG
AACGAGCGGGTGGTGGTGGAAAGCGGCCTGAAGGCCGGCGAGCAGGTGGTGGTGGAAGGCACCGACCGCCTG
CGCGACGGTATGGAAGTGCGTGTCGCCGAGGCCTCCCCGCAGGTCCTCGAGGGCGAGCCGCAGAAACCGCAG
ACTGGCCGCCCCAGCGGCCTCCAGGGCGACTCGGTGGGTAGCGGGAGCGCTGAATGA
Translation:
MTPTTGKSKFRTLRPWLITALAFAAVIGLVMWLAAPASAPSSDGRPGRGGKPGAALPKANALTVGVARVEQG
DLALHFNALGTVTAFNTVNVKPRVNGELVKVLFQEGQEVKAGDLLAVVDPRTYKAALAQAEGTLMQNQAQLK
NAEIDLQRYKGLYAEDSIAKQTLDTQEAQVRQLQGTIRTNQGQVDDARLNLTFTEVRAPISGRLGLRQVDIG
NLVTSGDTTPLVVITQVKPISVVFSLPQQQIGTVVEQMNGPGKLAVTALDRNQDKVLAEGTLTTLDNQIDTT
TGTVKLKARFENADGKLFPNQFVNVRLLAQTLKGVLTIPANAVQRGTNGIYVYVVGADNKVSQRSVAIGTSE
NERVVVESGLKAGEQVVVEGTDRLRDGMEVRVAEASPQVLEGEPQKPQTGRPSGLQGDSVGSGSAE*
AnnotationID:1384GeneID:1375
AnnotatorUID: diggins
Modification Date/Time: 2005-03-06 18:06:32
Gene Name:
Confidence Code:3
GeneProduct:putative RND efflux membrane fusion protein precursor
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:putative secretion protein
Functional Category Confidence Code:5
COGs:COG0845,pfam00529
Secondary Functional Category(ies):protein secretion/export apparatus
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN69185.1|AE016552_7 (AE016787) RND transporter, membrane fusion protein [Pseudomonas
           putida KT2440]
          Length = 435

 Score =  430 bits (1106), Expect = e-120
 Identities = 235/433 (54%), Positives = 294/433 (67%), Gaps = 16/433 (3%)

>gb|AAO56254.1| (AE016865) HlyD family secretion protein [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 444

 Score =  413 bits (1061), Expect = e-114
 Identities = 226/420 (53%), Positives = 279/420 (66%), Gaps = 6/420 (1%)

>emb|CAD18348.1| (AL646083) PUTATIVE RESISTANCE TRANSMEMBRANE PROTEIN [Ralstonia
           solanacearum]
          Length = 449

 Score =  369 bits (946), Expect = e-101
 Identities = 201/415 (48%), Positives = 274/415 (66%), Gaps = 9/415 (2%)






 
Structural Features:
COG0845, AcrA, Membrane-fusion protein [Cell envelope biogenesis, outer membrane]
CD-Length = 372 residues,  92.5% aligned
Score =  142 bits (358), Expect = 8e-35
Identities = 126/350 (36%), Positives = 174/350 (49%), Gaps = 10/350 (2%)

pfam00529, HlyD, HlyD family secretion protein.
CD-Length = 309 residues, 100.0% aligned
Score = 85.9 bits (212), Expect = 7e-18
Identities = 64/309 (20%), Positives = 112/309 (36%), Gaps = 18/309 (5%)







   
Genomic Context:






   
Comment:


















    ReferenceID:2753




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19898




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33090




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1375_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:1375
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2510
                           PA2528_tr
                           translation of PA2528
                           426
                           99.76
                           99.76
                           0
                           2117.0
     

     
     
79374
                           gb|AAG05916.1|AE004680_4
                           (AE004680) probable RND efflux membrane fusion protein precursor [Pseudomonas aeruginosa PAO1]
                           426
                           99.76
                           99.76
                           0
                           2117.0
     

     
     
79375
                           gb|AAN69185.1|AE016552_7
                           (AE016787) RND transporter, membrane fusion protein [Pseudomonas putida KT2440]
                           443
                           53.72
                           67.04
                           25
                           1119.0
     

     
     
79376
                           gb|AAO56254.1|
                           (AE016865) HlyD family secretion protein [Pseudomonas syringae pv. tomato str. DC3000]
                           449
                           50.55
                           62.80
                           28
                           1064.0
     

     
     
79377
                           emb|CAE39242.1|
                           (BX640435) HlyD family secretion protein [Bordetella parapertussis]
                           442
                           47.51
                           61.76
                           42
                           963.0
     

     






      

          
GC ORFID: 44377How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2528Glimmer Score: 

          
Start: 2181871Stop: 2180591

          
Length: 1281


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2528 translation of PA2528Homolog Bit Score 820.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene1337 (ORF: BLASTX 411.0), t_PA1435 (ORF: BLASTX 272.0), t_PA2528 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACTCCAACGACCGGTAAATCCAAGTTCCGTACCCTGCGCCCGTGGCTGATCACCGCCCTGGCCTTCGCC 
GCCGTGATCGGCCTGGTGATGTGGCTGGCGGCGCCCGCCTCGGCACCGTCCTCCGACGGGCGACCCGGTCGC 
GGCGGCAAGCCGGGCGCCGCGCTGCCCAAGGCCAACGCGCTCACCGTCGGCGTGGCCAGGGTGGAGCAGGGC 
GACCTGGCGCTGCATTTCAACGCGCTTGGCACCGTCACCGCCTTCAACACGGTGAACGTCAAGCCGCGGGTC 
AACGGCGAGCTGGTCAAGGTGCTGTTCCAGGAGGGGCAGGAGGTCAAGGCCGGCGACCTGCTGGCGGTGGTC 
GACCCGCGTACCTACAAGGCGGCGCTGGCCCAGGCCGAGGGCACGCTGATGCAGAACCAGGCGCAACTGAAG 
AACGCCGAGATCGACCTGCAGCGCTACAAGGGGCTGTACGCCGAGGACTCGATCGCCAAGCAGACCCTGGAT 
ACCCAGGAAGCCCAGGTCCGCCAGTTGCAGGGCACCATCCGTACCAACCAGGGCCAGGTCGACGACGCCCGC 
CTCAACCTGACCTTCACCGAGGTCCGCGCACCGATTTCCGGGCGCCTCGGCCTGCGCCAGGTGGACATCGGC 
AACCTGGTCACCAGCGGCGATACCACGCCGCTGGTGGTGATCACCCAGGTCAAGCCGATCTCGGTGGTGTTC 
AGCCTGCCGCAGCAGCAGATCGGCACCGTCGTCGAGCAGATGAACGGCCCCGGCAAGCTGGCGGTCACCGCG 
CTGGACCGCAACCAGGACAAGGTTCTCGCCGAAGGCACCCTGACCACCCTGGACAACCAGATCGACACCACC 
ACCGGCACGGTCAAGCTCAAGGCACGCTTCGAGAACGCCGACGGCAAGCTGTTCCCCAACCAGTTCGTCAAC 
GTGCGCCTGCTGGCGCAGACCCTCAAGGGCGTGCTGACCATTCCGGCCAACGCCGTGCAGCGCGGCACCAAC 
GGTATCTATGTGTACGTGGTCGGCGCCGACAACAAGGTCAGCCAGCGCAGCGTCGCCATCGGCACCAGCGAG 
AACGAGCGGGTGGTGGTGGAAAGCGGCCTGAAGGCCGGCGAGCAGGTGGTGGTGGAAGGCACCGACCGCCTG 
CGCGACGGTATGGAAGTGCGTGTCGCCGAGGCCTCCCCGCAGGTCCTCGAGGGCGAGCCGCAGAAACCGCAG 
ACTGGCCGCCCCAGCGGCCTCCAGGGCGACTCGGTGGGTAGCGGGAGCGCTGAATGA