ViewGene
Locus Name:
GeneID:
Location:

GeneID:1124
TrivialName: PA14_38510
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_38510
PAO1 Ortholog Locus:PA2009
Sequence Length:1299
Protein Length:432
Start: 3435469
Stop: 3436767
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05397.1|AE004627_5 (AE004627) homogentisate 1,2-dioxygenase [Pseudomonas aeruginosa PAO1]
Identities = 430/432 (99%), Positives = 430/432 (99%)
Sequence:
ATGAACCTCGACTCCACTGCCCTCGCCTATCAATCGGGCTTCGGCAACGAATTCAGCAGCGAAGCGCTCCCC
GGCGCCCTGCCGGTCGGCCAGAACTCCCCGCAGAAAGCGCCCTACGGCCTGTACGCCGAACTGCTCTCCGGC
ACCGCCTTCACCATGGCTCGCAGCGAGGCCCGGCGCACCTGGCTATACCGCATCACGCCGTCGGCCAAGCAT
CCGCCGTTCCGCCGCCTGGAACGACAGATCGCCGGTGCCGAACTGGATGCGCCGACCCCCAACCGCCTGCGC
TGGGACCCGCTGGCACTGCCCGAGCAGCCCACCGACTTCCTCGACGGCCTGCTGCGCATGGCCGCCAACGCG
CCCGGCGACAAGCCCGCCGGCGTGAGCATCTACCAGTACCTGGCCAACCGCTCGATGGAGCGTTGCTTCTAC
GACGCCGACGGCGAACTGCTGCTGGTCCCGCAGTTGGGCCGCCTGCGCCTGTGCACCGAACTCGGCGCGCTG
CAGGTCGAACCGCTGGAGATCGCGGTGATCCCGCGCGGGATGAAGTTCCGCGTCGAGCTGCTCGACGGCGAG
GCACGCGGCTATATCGCCGAGAACCACGGCGCGCCGCTGCGCCTGCCCGACCTCGGCCCGATCGGCAGCAAT
GGCCTGGCCAATCCGCGCGACTTCCTGGCCCCGGTGGCGCGCTACGAAGACAGCCGCCAGCCGCTGCAACTG
GTGCAGAAATACCTCGGCGAGCTGTGGGCCTGCGAGCTTGACCACTCGCCGCTGGACGTGGTCGCCTGGCAC
GGCAACAACGTGCCCTACAAGTACGACCTGCGCCGCTTCAACACCATCGGCACGGTCAGCTTCGACCACCCG
GACCCGTCGATCTTCACCGTGCTGACCTCCCCCACCAGCGTCCATGGCCTGGCCAACATCGACTTCGTGATC
TTCCCGCCGCGCTGGATGGTGGCCGAGAACACCTTCCGTCCGCCATGGTTCCACCGCAACCTGATGAACGAG
TTCATGGGCCTGATCCAGGGCGCCTATGACGCCAAGGCCGGCGGCTTCGTGCCTGGCGGCGCCTCGCTGCAC
AGTTGCATGAGCGCCCACGGCCCGGACGCGGAAAGCTGCGACAAGGCCATCGCCGCCGACCTCAAGCCGCAC
AGGATCGACCAGACCATGGCCTTCATGTTCGAGACCAGCCAGGTCCTCCGGCCGAGCCGTGCCGCCCTCGAG
ACGCCGGCCCTGCAGAATGACTACGATGCCTGCTGGGCGTCGCTCGTATCCACCTTCAACCCGCAACGGAGA
TAA
Translation:
MNLDSTALAYQSGFGNEFSSEALPGALPVGQNSPQKAPYGLYAELLSGTAFTMARSEARRTWLYRITPSAKH
PPFRRLERQIAGAELDAPTPNRLRWDPLALPEQPTDFLDGLLRMAANAPGDKPAGVSIYQYLANRSMERCFY
DADGELLLVPQLGRLRLCTELGALQVEPLEIAVIPRGMKFRVELLDGEARGYIAENHGAPLRLPDLGPIGSN
GLANPRDFLAPVARYEDSRQPLQLVQKYLGELWACELDHSPLDVVAWHGNNVPYKYDLRRFNTIGTVSFDHP
DPSIFTVLTSPTSVHGLANIDFVIFPPRWMVAENTFRPPWFHRNLMNEFMGLIQGAYDAKAGGFVPGGASLH
SCMSAHGPDAESCDKAIAADLKPHRIDQTMAFMFETSQVLRPSRAALETPALQNDYDACWASLVSTFNPQRR
*
AnnotationID:1133GeneID:1124
AnnotatorUID: diggins
Modification Date/Time: 2005-04-28 17:05:38
Gene Name:hmgA
Confidence Code:2
GeneProduct:homogentisate 1,2-dioxygenase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(5) Carbon compound catabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam04209,COG3508
Secondary Functional Category(ies):central intermediary metabolism
EC Number:1.13.11.5
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70194.1|AE016658_11 (AE016791) homogentisate 1,2-dioxygenase [Pseudomonas putida
           KT2440]
          Length = 433

 Score =  728 bits (1879), Expect = 0.0
 Identities = 353/433 (81%), Positives = 381/433 (87%), Gaps = 1/433 (0%)

>gb|AAK24503.1| (AE005921) homogentisate 1,2-dioxygenase [Caulobacter crescentus
           CB15]
          Length = 425

 Score =  558 bits (1439), Expect = e-158
 Identities = 271/415 (65%), Positives = 320/415 (77%), Gaps = 3/415 (0%)

>gb|AAM39756.1| (AE012141) homogentisate 1,2-dioxygenase [Xanthomonas campestris
           pv. campestris str. ATCC 33913]
          Length = 455

 Score =  546 bits (1406), Expect = e-154
 Identities = 267/422 (63%), Positives = 315/422 (74%), Gaps = 4/422 (0%)






 
Structural Features:
pfam04209, HgmA, homogentisate 1,2-dioxygenase.
CD-Length = 425 residues, 100.0% aligned
Score =  647 bits (1671), Expect = 0.0
Identities = 269/426 (63%), Positives = 318/426 (74%), Gaps = 6/426 (1%)

COG3508, HmgA, Homogentisate 1,2-dioxygenase 
[Secondary metabolites biosynthesis, transport, and catabolism]
CD-Length = 427 residues,  99.1% aligned
Score =  633 bits (1635), Expect = 0.0
Identities = 300/426 (70%), Positives = 332/426 (77%), Gaps = 6/426 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:2251




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19393




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32720




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1124_cdd.html


    		




 
URL:

















    ReferenceID:34383




  
Author/Investigator(s):
        Milcamps A, de Bruijn FJ.





  
Title:
        Identification of a novel nutrient-deprivation-induced Sinorhizobium meliloti gene (hmgA) involved in the degradation of tyrosine.




  
PubMed:
        10220173


MedLine:
        




    
Source:
Microbiology. 1999 Apr;145 ( Pt 4):935-47.






    
Reference Type:Journal 







    
Data:


    		




 
URL:ttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10220173

















    ReferenceID:34384




  
Author/Investigator(s):
        Bochar DA, Brown JR, Doolittle WF, Klenk HP, Lam W, Schenk ME, Stauffacher CV, Rodwell VW.





  
Title:
        3-hydroxy-3-methylglutaryl coenzyme A reductase of Sulfolobus solfataricus: DNA sequence, phylogeny, expression in Escherichia coli of the hmgA gene, and purification and kinetic characterization of the gene product.




  
PubMed:
        9171410


MedLine:
        




    
Source:
J Bacteriol. 1997 Jun;179(11):3632-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=9171410









           
Homologs By Global Alignment 

           

       



     
Gene ID:1124
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2019
                           PA2009_tr
                           translation of PA2009
                           432
                           99.53
                           99.53
                           0
                           2285.0
     

     
     
68319
                           gb|AAG05397.1|AE004627_5
                           (AE004627) homogentisate 1,2-dioxygenase [Pseudomonas aeruginosa PAO1]
                           432
                           99.53
                           99.53
                           0
                           2285.0
     

     
     
68320
                           gb|AAN70194.1|AE016658_11
                           (AE016791) homogentisate 1,2-dioxygenase [Pseudomonas putida KT2440]
                           433
                           81.75
                           87.75
                           1
                           1881.0
     

     
     
68321
                           gb|AAO57026.1|
                           (AE016868) homogentisate 1,2-dioxygenase [Pseudomonas syringae pv. tomato str. DC3000]
                           434
                           70.73
                           82.02
                           2
                           1674.0
     

     
     
68322
                           gb|AAK24503.1|
                           (AE005921) homogentisate 1,2-dioxygenase [Caulobacter crescentus CB15]
                           436
                           62.61
                           74.08
                           15
                           1442.0
     

     






      

          
GC ORFID: 44126How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2009Glimmer Score: 

          
Start: 1518442Stop: 1517144

          
Length: 1299


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA2009 translation of PA2009Homolog Bit Score 884.0

          
Other Homologs:  psyr_15may02_Scaffold9_revised_gene8018 (ORF: BLASTX 662.0), t_PA2009 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAACCTCGACTCCACTGCCCTCGCCTATCAATCGGGCTTCGGCAACGAATTCAGCAGCGAAGCGCTCCCC 
GGCGCCCTGCCGGTCGGCCAGAACTCCCCGCAGAAAGCGCCCTACGGCCTGTACGCCGAACTGCTCTCCGGC 
ACCGCCTTCACCATGGCTCGCAGCGAGGCCCGGCGCACCTGGCTATACCGCATCACGCCGTCGGCCAAGCAT 
CCGCCGTTCCGCCGCCTGGAACGACAGATCGCCGGTGCCGAACTGGATGCGCCGACCCCCAACCGCCTGCGC 
TGGGACCCGCTGGCACTGCCCGAGCAGCCCACCGACTTCCTCGACGGCCTGCTGCGCATGGCCGCCAACGCG 
CCCGGCGACAAGCCCGCCGGCGTGAGCATCTACCAGTACCTGGCCAACCGCTCGATGGAGCGTTGCTTCTAC 
GACGCCGACGGCGAACTGCTGCTGGTCCCGCAGTTGGGCCGCCTGCGCCTGTGCACCGAACTCGGCGCGCTG 
CAGGTCGAACCGCTGGAGATCGCGGTGATCCCGCGCGGGATGAAGTTCCGCGTCGAGCTGCTCGACGGCGAG 
GCACGCGGCTATATCGCCGAGAACCACGGCGCGCCGCTGCGCCTGCCCGACCTCGGCCCGATCGGCAGCAAT 
GGCCTGGCCAATCCGCGCGACTTCCTGGCCCCGGTGGCGCGCTACGAAGACAGCCGCCAGCCGCTGCAACTG 
GTGCAGAAATACCTCGGCGAGCTGTGGGCCTGCGAGCTTGACCACTCGCCGCTGGACGTGGTCGCCTGGCAC 
GGCAACAACGTGCCCTACAAGTACGACCTGCGCCGCTTCAACACCATCGGCACGGTCAGCTTCGACCACCCG 
GACCCGTCGATCTTCACCGTGCTGACCTCCCCCACCAGCGTCCATGGCCTGGCCAACATCGACTTCGTGATC 
TTCCCGCCGCGCTGGATGGTGGCCGAGAACACCTTCCGTCCGCCATGGTTCCACCGCAACCTGATGAACGAG 
TTCATGGGCCTGATCCAGGGCGCCTATGACGCCAAGGCCGGCGGCTTCGTGCCTGGCGGCGCCTCGCTGCAC 
AGTTGCATGAGCGCCCACGGCCCGGACGCGGAAAGCTGCGACAAGGCCATCGCCGCCGACCTCAAGCCGCAC 
AGGATCGACCAGACCATGGCCTTCATGTTCGAGACCAGCCAGGTCCTCCGGCCGAGCCGTGCCGCCCTCGAG 
ACGCCGGCCCTGCAGAATGACTACGATGCCTGCTGGGCGTCGCTCGTATCCACCTTCAACCCGCAACGGAGA 
TAA