ViewGene
Locus Name:
GeneID:
Location:

GeneID:1075
TrivialName: PA14_60710
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_60710
PAO1 Ortholog Locus:PA4588
Sequence Length:1338
Protein Length:445
Start: 5411222
Stop: 5409885
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07976.1|AE004872_7 (AE004872) glutamate dehydrogenase [Pseudomonas aeruginosa PAO1]
Identities = 445/445 (100%), Positives = 445/445 (100%)
Sequence:
ATGACGCAATCCGTCGACGCTTTCCTCGAGCGCCTGAAGCGGCGCGATCCCGATCAGCCCGAATTCCACCAG
GCGGTGGAAGAGGTGCTGCGTTCCCTCTGGCCGTTCCTCGAGGCCAACCCGCACTACCTGGAAGCCGGCATC
ATCGAACGCATCGTCGAACCCGAGCGGGCGATCCTGTTCCGCGTACCCTGGGTCGACGACCAGGGCCGGGTA
CGGGTCAACCGTGGCTACCGGGTGCAGATGAGCAGCGCCATCGGCCCCTACAAGGGCGGCCTGCGCTTCCAT
CCCTCGGTCAACCTCGGGGTGCTGAAGTTCCTCGCCTTCGAGCAGGTCTTCAAGAACTCCCTGACCACTCTG
CCCATGGGCGGCGGCAAGGGCGGCTCCGACTTCGATCCGAAGGGCAAGAGCGACGCCGAGGTGATGCGTTTC
TGCCAGTCGTTCATGAGCGAGCTGTACCGCCACGTCGGCGCCGACCTGGACGTGCCGGCCGGCGACATCGGC
GTCGGCGCGCGTGAGATCGGCTACCTGTTCGGCCAGTACAAGCGCCTGTCCAACCAGTTCACCTCGGTGCTG
ACCGGCAAGGGCCTGAGCTACGGCGGCAGCCTGATCCGCCCGGAGGCGACCGGCTTCGGCTGCGTCTACTTC
GCCCAGGAAATGCTCAAGGATCGTGGCCGCGGCTTCGACGGCCAGCGGGTGGCGATCTCCGGCTCGGGCAAC
GTCGCCCAGTACGCGGCGCGCAAGGTCATGGAAATGGGCGGCAAGGTGATCTCGCTGTCCGACTCCGAAGGC
ACCCTGTATGCCGAGGCGGGGCTCTCCGACGAGCAGTGGGAATACCTGATGGAGCTGAAGAACGTGCGCCGC
GGGCGCATCCGCGAGATGGCGGAGCAGTTTTCCCTGCAGTTCCTCGAAGGGCGCCGGCCCTGGGGCCTGGCC
TGCGATATCGCGCTGCCCTGCGCGACCCAGAACGAACTGGATGCCGAGGATGCGCGGCGCCTGCTGGCCAAC
GGCTGCGTCTGTGTCGCCGAAGGCGCCAACATGCCCTCGACCCTGGAAGCCGTCGACCTGTTCCTCGAGGCC
GGCATCCTCTACGCGCCGGGCAAGGCGTCGAACGCCGGCGGGGTGGCGGTGAGCGGTCTGGAGATGTCGCAG
AACGCCATGCGCCTGCGTTGGAGCGAGGGCGAGGTGGATACCAAGCTGCACGGCATCATGCAGTCGATCCAC
CATGCCTGCCTGCTGTACGGCGAGGAGCAGGGGCGGGTCAACTACGTGAAGGGGGCGAACATCGCCGGCTTC
GTCAAGGTCGCCGATGCGATGCTGGCGCAGGGCGTGGTCTGA
Translation:
MTQSVDAFLERLKRRDPDQPEFHQAVEEVLRSLWPFLEANPHYLEAGIIERIVEPERAILFRVPWVDDQGRV
RVNRGYRVQMSSAIGPYKGGLRFHPSVNLGVLKFLAFEQVFKNSLTTLPMGGGKGGSDFDPKGKSDAEVMRF
CQSFMSELYRHVGADLDVPAGDIGVGAREIGYLFGQYKRLSNQFTSVLTGKGLSYGGSLIRPEATGFGCVYF
AQEMLKDRGRGFDGQRVAISGSGNVAQYAARKVMEMGGKVISLSDSEGTLYAEAGLSDEQWEYLMELKNVRR
GRIREMAEQFSLQFLEGRRPWGLACDIALPCATQNELDAEDARRLLANGCVCVAEGANMPSTLEAVDLFLEA
GILYAPGKASNAGGVAVSGLEMSQNAMRLRWSEGEVDTKLHGIMQSIHHACLLYGEEQGRVNYVKGANIAGF
VKVADAMLAQGVV*
AnnotationID:1084GeneID:1075
AnnotatorUID: diggins
Modification Date/Time: 2005-05-06 05:05:39
Gene Name:gdhA
Confidence Code:2
GeneProduct:NADP-glutamate dehydrogenase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0334,KOG2250,pfam00208
Secondary Functional Category(ies):
EC Number:1.4.1.4
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAF42057.1| (AE002521) glutamate dehydrogenase, NADP-specific [Neisseria
           meningitidis MC58]
          Length = 444

 Score =  638 bits (1645), Expect = 0.0
 Identities = 312/440 (70%), Positives = 370/440 (84%), Gaps = 1/440 (0%)

>gb|AAG56747.1|AE005399_1 (AE005399) NADP-specific glutamate dehydrogenase [Escherichia coli
           O157:H7 EDL933]
          Length = 447

 Score =  606 bits (1563), Expect = e-173
 Identities = 289/444 (65%), Positives = 358/444 (80%)







 
Structural Features:
COG0334, GdhA, Glutamate dehydrogenase/leucine dehydrogenase 
[Amino acid transport and metabolism]
CD-Length = 411 residues, 100.0% aligned
Score =  521 bits (1344), Expect = 1e-148
Identities = 188/428 (43%), Positives = 253/428 (59%), Gaps = 18/428 (4%)

KOG2250, Glutamate/leucine/phenylalanine/valine dehydrogenases 
[Amino acid transport and metabolism]
CD-Length = 514 residues,  87.2% aligned
Score =  434 bits (1118), Expect = 2e-122
Identities = 186/450 (41%), Positives = 254/450 (56%), Gaps = 24/450 (5%)

pfam00208, GLFV_dehydrog, Glutamate/Leucine/Phenylalanine/Valine dehydrogenase.
CD-Length = 238 residues, 100.0% aligned
Score =  297 bits (762), Expect = 3e-81
Identities = 120/244 (49%), Positives = 155/244 (63%), Gaps = 6/244 (2%)







   
Genomic Context:






   
Comment:


















    ReferenceID:2153




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19295




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31632




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1075_cdd.html


    		




 
URL:

















    ReferenceID:33209




  
Author/Investigator(s):
        Lu CD, Yang Z, Li W.





  
Title:
        Transcriptome analysis of the ArgR regulon in Pseudomonas aeruginosa.




  
PubMed:
        15175299


MedLine:
        




    
Source:
J Bacteriol. 2004 Jun;186(12):3855-61.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15175299

















    ReferenceID:33210




  
Author/Investigator(s):
        Hashim S, Kwon DH, Abdelal A, Lu CD.





  
Title:
        The arginine regulatory protein mediates repression by arginine of the operons encoding glutamate synthase and anabolic glutamate dehydrogenase in Pseudomonas aeruginosa.




  
PubMed:
        15175298


MedLine:
        




    
Source:
J Bacteriol. 2004 Jun;186(12):3848-54.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15175298

















    ReferenceID:33211




  
Author/Investigator(s):
        Joannou CL, Brown PR, Tata R.





  
Title:
        Mutations affecting the synthesis of NADP-dependent glutamate dehydrogenase in Pseudomonas aeruginosa.




  
PubMed:
        2844962


MedLine:
        




    
Source:
J Gen Microbiol. 1988 Feb;134 ( Pt 2):441-52.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2844962









           
Homologs By Global Alignment 

           

       



     
Gene ID:1075
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1937
                           PA4588_tr
                           translation of PA4588
                           445
                           99.99
                           99.99
                           0
                           2308.0
     

     
     
66802
                           gb|AAG07976.1|AE004872_7
                           (AE004872) glutamate dehydrogenase [Pseudomonas aeruginosa PAO1]
                           445
                           99.99
                           99.99
                           0
                           2308.0
     

     
     
66803
                           emb|CAA77192.1|
                           (Y18494) NADP-glutamate dehydrogenase [Pseudomonas aeruginosa]
                           445
                           97.97
                           98.20
                           0
                           2256.0
     

     
     
66804
                           emb|CAA75437.1|
                           (Y15166) NADP-glutamate dehydrogenase [Pseudomonas aeruginosa]
                           445
                           97.97
                           98.20
                           0
                           2256.0
     

     
     
66805
                           gb|AAN66300.1|AE016258_6
                           (AE016776) glutamate dehydrogenase [Pseudomonas putida KT2440]
                           449
                           83.51
                           92.87
                           4
                           2002.0
     

     






      

          
GC ORFID: 44077How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4588Glimmer Score: 

          
Start: 4674902Stop: 4673565

          
Length: 1338


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4588 translation of PA4588Homolog Bit Score 893.0

          
Other Homologs:  t_PA4588 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACGCAATCCGTCGACGCTTTCCTCGAGCGCCTGAAGCGGCGCGATCCCGATCAGCCCGAATTCCACCAG 
GCGGTGGAAGAGGTGCTGCGTTCCCTCTGGCCGTTCCTCGAGGCCAACCCGCACTACCTGGAAGCCGGCATC 
ATCGAACGCATCGTCGAACCCGAGCGGGCGATCCTGTTCCGCGTACCCTGGGTCGACGACCAGGGCCGGGTA 
CGGGTCAACCGTGGCTACCGGGTGCAGATGAGCAGCGCCATCGGCCCCTACAAGGGCGGCCTGCGCTTCCAT 
CCCTCGGTCAACCTCGGGGTGCTGAAGTTCCTCGCCTTCGAGCAGGTCTTCAAGAACTCCCTGACCACTCTG 
CCCATGGGCGGCGGCAAGGGCGGCTCCGACTTCGATCCGAAGGGCAAGAGCGACGCCGAGGTGATGCGTTTC 
TGCCAGTCGTTCATGAGCGAGCTGTACCGCCACGTCGGCGCCGACCTGGACGTGCCGGCCGGCGACATCGGC 
GTCGGCGCGCGTGAGATCGGCTACCTGTTCGGCCAGTACAAGCGCCTGTCCAACCAGTTCACCTCGGTGCTG 
ACCGGCAAGGGCCTGAGCTACGGCGGCAGCCTGATCCGCCCGGAGGCGACCGGCTTCGGCTGCGTCTACTTC 
GCCCAGGAAATGCTCAAGGATCGTGGCCGCGGCTTCGACGGCCAGCGGGTGGCGATCTCCGGCTCGGGCAAC 
GTCGCCCAGTACGCGGCGCGCAAGGTCATGGAAATGGGCGGCAAGGTGATCTCGCTGTCCGACTCCGAAGGC 
ACCCTGTATGCCGAGGCGGGGCTCTCCGACGAGCAGTGGGAATACCTGATGGAGCTGAAGAACGTGCGCCGC 
GGGCGCATCCGCGAGATGGCGGAGCAGTTTTCCCTGCAGTTCCTCGAAGGGCGCCGGCCCTGGGGCCTGGCC 
TGCGATATCGCGCTGCCCTGCGCGACCCAGAACGAACTGGATGCCGAGGATGCGCGGCGCCTGCTGGCCAAC 
GGCTGCGTCTGTGTCGCCGAAGGCGCCAACATGCCCTCGACCCTGGAAGCCGTCGACCTGTTCCTCGAGGCC 
GGCATCCTCTACGCGCCGGGCAAGGCGTCGAACGCCGGCGGGGTGGCGGTGAGCGGTCTGGAGATGTCGCAG 
AACGCCATGCGCCTGCGTTGGAGCGAGGGCGAGGTGGATACCAAGCTGCACGGCATCATGCAGTCGATCCAC 
CATGCCTGCCTGCTGTACGGCGAGGAGCAGGGGCGGGTCAACTACGTGAAGGGGGCGAACATCGCCGGCTTC 
GTCAAGGTCGCCGATGCGATGCTGGCGCAGGGCGTGGTCTGA