ViewGene
Locus Name:
GeneID:
Location:

GeneID:1052
TrivialName: PA14_35490
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_35490
PAO1 Ortholog Locus:PA2250
Sequence Length:1395
Protein Length:464
Start: 3156056
Stop: 3154662
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05638.1|AE004650_9 (AE004650) lipoamide dehydrogenase-Val [Pseudomonas aeruginosa PAO1]
Identities = 462/464 (99%), Positives = 463/464 (99%)
Sequence:
ATGAGCCAGATCCTGAAGACTTCCCTGCTGATCGTCGGCGGCGGTCCCGGCGGCTACGTCGCGGCGATCCGT
GCCGGGCAACTGGGCATTCCCACCGTACTGGTGGAGGGCGCCGCCCTCGGCGGCACCTGCCTGAACGTCGGC
TGCATCCCGTCGAAGGCGCTGATCCACGCCGCCGAGGAATACCTCAAGGCCCGCCACTATGCCGGCCGGTCG
GCGCTGGGCATCCAGGTACAGGCGCCGAGCATCGACATCGCCCGCACCGTGGAATGGAAGGACGCCATCGTC
GACCGCCTCACCAGCGGCGTCGCCGCGCTGCTGAAGAAACACGGGGTCGATGTCGTCCAGGGCTGGGCGAGG
ATCCTCGACGGCAAAAGCGTGGCGGTCGAACTCGCCGGCGGCGGCAGCCAGCGCATCGAGTGCGAGCACCTG
CTGCTGGCCGCAGGCTCGCAGAGCGTCGAGCTACCGATCCTGCCGCTGGGCGGCAAGGTGATCTCCTCCACC
GAGGCGCTGGCGCCCGGCAGCCTGCCCAAGCGCCTGGTGGTGGTCGGCGGCGGCTACATCGGCCTGGAGCTG
GGTACCGCCTACCGCAAGCTCGGCGTCGAGGTGGCGGTGGTGGAAGCGCAACCGCGCATCCTGCCGGGCTAC
GACGAAGAACTGACCAAGCCGGTGGCCCAGGCCTTGCGCAAGCTGGGCGTCGAGCTGTACCTCGGGCACAGC
CTGCTGGGCCCGAGCGAGAACGGCGTGCGGGTCCGCGACGGCGCCGGCGAGGAGCGCGAGATCGCCGCCGAC
CAGGTACTGGTGGCGGTCGGCCGCAAGCCGCGCAGCGAAGGCTGGAACCTGGAAAGCCTGGGCCTGGACATG
AACGGCCGGGCGGTGAAGGTCGACGACCAGTGCCGCACCTCGATGCGCAATGTCTGGGCCATCGGCGATCTC
GCCGGCGAGCCGATGCTCGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAGCTGATCGCCGGCAAG
CGTCGCCAGTTCGCCCCGGTGGCGATCCCCGCGGTGTGCTTCACCGATCCGGAAGTGGTGGTCGCCGGGTTG
TCCCCGGAGCAGGCGAAGGATGCCGGCCTGGACTGCCTGGTGGCGAGCTTCCCGTTCGCCGCCAACGGCCGC
GCCATGACCCTGGAGGCCAACGAAGGCTTCGTCCGCGTGGTGGCGCGTCGCGACAACCACCTGGTCGTCGGC
TGGCAGGCGGTGGGCAAGGCGGTCTCGGAACTGTCCACGGCCTTCGCCCAGTCGCTGGAGATGGGCGCCCGC
CTGGAAGACATCGCCGGCACCATCCACGCCCATCCGACCCTCGGCGAAGCGGTCCAGGAAGCCGCCCTGCGC
GCGCTGGGACACGCCCTGCACATCTGA
Translation:
MSQILKTSLLIVGGGPGGYVAAIRAGQLGIPTVLVEGAALGGTCLNVGCIPSKALIHAAEEYLKARHYAGRS
ALGIQVQAPSIDIARTVEWKDAIVDRLTSGVAALLKKHGVDVVQGWARILDGKSVAVELAGGGSQRIECEHL
LLAAGSQSVELPILPLGGKVISSTEALAPGSLPKRLVVVGGGYIGLELGTAYRKLGVEVAVVEAQPRILPGY
DEELTKPVAQALRKLGVELYLGHSLLGPSENGVRVRDGAGEEREIAADQVLVAVGRKPRSEGWNLESLGLDM
NGRAVKVDDQCRTSMRNVWAIGDLAGEPMLAHRAMAQGEMVAELIAGKRRQFAPVAIPAVCFTDPEVVVAGL
SPEQAKDAGLDCLVASFPFAANGRAMTLEANEGFVRVVARRDNHLVVGWQAVGKAVSELSTAFAQSLEMGAR
LEDIAGTIHAHPTLGEAVQEAALRALGHALHI*
AnnotationID:1061GeneID:1052
AnnotatorUID: diggins
Modification Date/Time: 2005-04-29 06:06:46
Gene Name:lpdV
Confidence Code:2
GeneProduct:lipoamide dehydrogenase-Val
Cell Localization:()
Synonyms:pdhD
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(12) Energy metabolism
Alternate Gene Product Name:dihydrolipoamide dehydrogenase E3 subunit
Functional Category Confidence Code:5
COGs:COG1249
Secondary Functional Category(ies):amino acid biosynthesis and metabolims
EC Number:1.8.1.4
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN69982.1|AE016636_5 (AE016790) 2-oxoisovalerate dehydrogenase, lipoamide dehydrogenase
           component [Pseudomonas putida KT2440]
          Length = 459

 Score =  679 bits (1751), Expect = 0.0
 Identities = 341/464 (73%), Positives = 391/464 (84%), Gaps = 5/464 (1%)

>dbj|BAB51120.1| (AP003004) dihydrolipoamide dehydrogenase [Mesorhizobium loti]
          Length = 464

 Score =  561 bits (1446), Expect = e-159
 Identities = 291/463 (62%), Positives = 347/463 (74%), Gaps = 5/463 (1%)

>gb|AAA62684.1| (M57435) dihydrolipoamide dehydrogenase E3 subunit [Bacillus
           subtilis]
          Length = 470

 Score =  391 bits (1005), Expect = e-108
 Identities = 209/465 (44%), Positives = 304/465 (65%), Gaps = 9/465 (1%)








 
Structural Features:
COG1249, Lpd, Pyruvate/2-oxoglutarate dehydrogenase complex, 
dihydrolipoamide dehydrogenase (E3) component, 
and related enzymes 
[Energy production and conversion]
CD-Length = 454 residues,  98.7% aligned
Score =  447 bits (1152), Expect = 1e-126
Identities = 216/452 (47%), Positives = 294/452 (65%), Gaps = 10/452 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:2107




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19249




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32525




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1052_cdd.html


    		




 
URL:

















    ReferenceID:34444




  
Author/Investigator(s):
        Burns G, Brown T, Hatter K, Sokatch JR.





  
Title:
        Sequence analysis of the lpdV gene for lipoamide dehydrogenase of branched-chain-oxoacid dehydrogenase of Pseudomonas putida.




  
PubMed:
        2917566


MedLine:
        




    
Source:
Eur J Biochem. 1989 Jan 15;179(1):61-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2917566









           
Homologs By Global Alignment 

           

       



     
Gene ID:1052
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1904
                           PA2250_tr
                           translation of PA2250
                           464
                           99.56
                           99.78
                           0
                           2326.0
     

     
     
65639
                           gb|AAG05638.1|AE004650_9
                           (AE004650) lipoamide dehydrogenase-Val [Pseudomonas aeruginosa PAO1]
                           464
                           99.56
                           99.78
                           0
                           2326.0
     

     
     
65640
                           gb|AAN69982.1|AE016636_5
                           (AE016790) 2-oxoisovalerate dehydrogenase, lipoamide dehydrogenase component [Pseudomonas putida KT2440]
                           465
                           73.97
                           84.73
                           7
                           1756.0
     

     
     
65641
                           gb|AAA65618.1|
                           (M57613) lipoamide dehydrogenase [Pseudomonas putida]
                           465
                           73.33
                           84.73
                           7
                           1746.0
     

     
     
65642
                           dbj|BAB51120.1|
                           (AP003004) dihydrolipoamide dehydrogenase [Mesorhizobium loti]
                           467
                           62.74
                           74.73
                           6
                           1453.0
     

     






      

          
GC ORFID: 44054How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2250Glimmer Score: 

          
Start: 1797860Stop: 1799254

          
Length: 1395


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2250 translation of PA2250Homolog Bit Score 900.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene999 (ORF: BLASTX 332.0), t_PA4829 (ORF: BLASTX 326.0), t_PA1587 (ORF: BLASTX 325.0), t_PA2250 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCCAGATCCTGAAGACTTCCCTGCTGATCGTCGGCGGCGGTCCCGGCGGCTACGTCGCGGCGATCCGT 
GCCGGGCAACTGGGCATTCCCACCGTACTGGTGGAGGGCGCCGCCCTCGGCGGCACCTGCCTGAACGTCGGC 
TGCATCCCGTCGAAGGCGCTGATCCACGCCGCCGAGGAATACCTCAAGGCCCGCCACTATGCCGGCCGGTCG 
GCGCTGGGCATCCAGGTACAGGCGCCGAGCATCGACATCGCCCGCACCGTGGAATGGAAGGACGCCATCGTC 
GACCGCCTCACCAGCGGCGTCGCCGCGCTGCTGAAGAAACACGGGGTCGATGTCGTCCAGGGCTGGGCGAGG 
ATCCTCGACGGCAAAAGCGTGGCGGTCGAACTCGCCGGCGGCGGCAGCCAGCGCATCGAGTGCGAGCACCTG 
CTGCTGGCCGCAGGCTCGCAGAGCGTCGAGCTACCGATCCTGCCGCTGGGCGGCAAGGTGATCTCCTCCACC 
GAGGCGCTGGCGCCCGGCAGCCTGCCCAAGCGCCTGGTGGTGGTCGGCGGCGGCTACATCGGCCTGGAGCTG 
GGTACCGCCTACCGCAAGCTCGGCGTCGAGGTGGCGGTGGTGGAAGCGCAACCGCGCATCCTGCCGGGCTAC 
GACGAAGAACTGACCAAGCCGGTGGCCCAGGCCTTGCGCAAGCTGGGCGTCGAGCTGTACCTCGGGCACAGC 
CTGCTGGGCCCGAGCGAGAACGGCGTGCGGGTCCGCGACGGCGCCGGCGAGGAGCGCGAGATCGCCGCCGAC 
CAGGTACTGGTGGCGGTCGGCCGCAAGCCGCGCAGCGAAGGCTGGAACCTGGAAAGCCTGGGCCTGGACATG 
AACGGCCGGGCGGTGAAGGTCGACGACCAGTGCCGCACCTCGATGCGCAATGTCTGGGCCATCGGCGATCTC 
GCCGGCGAGCCGATGCTCGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAGCTGATCGCCGGCAAG 
CGTCGCCAGTTCGCCCCGGTGGCGATCCCCGCGGTGTGCTTCACCGATCCGGAAGTGGTGGTCGCCGGGTTG 
TCCCCGGAGCAGGCGAAGGATGCCGGCCTGGACTGCCTGGTGGCGAGCTTCCCGTTCGCCGCCAACGGCCGC 
GCCATGACCCTGGAGGCCAACGAAGGCTTCGTCCGCGTGGTGGCGCGTCGCGACAACCACCTGGTCGTCGGC 
TGGCAGGCGGTGGGCAAGGCGGTCTCGGAACTGTCCACGGCCTTCGCCCAGTCGCTGGAGATGGGCGCCCGC 
CTGGAAGACATCGCCGGCACCATCCACGCCCATCCGACCCTCGGCGAAGCGGTCCAGGAAGCCGCCCTGCGC 
GCGCTGGGACACGCCCTGCACATCTGA