Mutant Report for Mutant ID: 56601
Strain: PA14 Genetic Background: Wild Type Transposon: MrT7 Library: lib_04_04
Raw Sequence ID: 31146
Chromat Path: H:\Pa14\MrT7\590v1\590v1_E09_E9_069.ab1 Last Transposon Base: 61 Transposon Sequence
Match Quality: 1
Raw Sequence:
(Transposon Sequence in Red)		 
TGTCGTTTGTAACGACTCAGTATAGTGTGCTAGAGACCGGAGACTTATCAGCCAACCTGTTACACCACCT
GGGCCGAGGCCGGCTGGAATTGCAGGCCGACCGCGCGGCGCGCGAGCTTCATCCATTTCTTCACGCGCTT
GGCGGCGTAGTGGATGCCGTGCAGGCTGGGCATGATCTCGGCGAGCAGGGTCTCGTCGGCGGAGCGCGCG
CTGAAGTCCCGGTCGATGGCCTCGATCAGTGCCTGCTGTTCCTTGAACAGCAGCTCGCGCAGGGCCTTCA
GCCACTGCACGCGCTGCGTGGCGTCGGGCATCGGCTGCGCCAGGTAGGCGGCGCGCTGGCGGGCGAAGAG
GGCTTCGAGCTGGTTGATTTCCTGCTGGCTCTGCTGCAGGTAGGCGATACCGGCGACCATCATCATCTGC
AGGCCCTGGCCAGGGGNGCGGCTGCTTGGTCGCGCGTCTGTCGTTATGGCGGACCTGGGTCGGTCCGATG
AATTTCGCTGCGTCCCTGGATTTCCAGAGTAATTACTCTTAAGTGTCATACAGCATGCCGTGTCGCCCCG
GGTGCTTGCGTACCCTGACATATGGGGGGTCGTTTCGCTTGCCGCCCCCCCTGGGGGCTCCCCCGGCCCG
CCCGTTTTTTTTCCCTCCTTCCCTTTCCCGCCAGGGTCTCTCCCCGTCTGTTTTCCTTATTTGGTCTTCC
TCCTCCTTTCACCCTTTTTCTTTTCTCCCCCTCTCATCTCCTGTTTTGTTGGGCTTTTTTGTTTTTCTGC
TTTTTTTTTCGCTCTACTTCTCACTTTTGTTTTTTTTTCTTCTCTTTTTTCTCTTTCCTGCTCTTTCCTT
GTTCTTCTTTTTTCTTTCCTGTCTCTGT
Blast against PA14
Processed Seqquence ID: 23948
5' Trim Position: 36 3' Trim Position: 424
Processed Sequence:
(Transposon Sequence in Red)		 
CCGGAGACTTATCAGCCAACCTGTTACACCACCTGGGCCGAGGCCGGCTGGAATTGCAGGCCGACCGCGC
GGCGCGCGAGCTTCATCCATTTCTTCACGCGCTTGGCGGCGTAGTGGATGCCGTGCAGGCTGGGCATGAT
CTCGGCGAGCAGGGTCTCGTCGGCGGAGCGCGCGCTGAAGTCCCGGTCGATGGCCTCGATCAGTGCCTGC
TGTTCCTTGAACAGCAGCTCGCGCAGGGCCTTCAGCCACTGCACGCGCTGCGTGGCGTCGGGCATCGGCT
GCGCCAGGTAGGCGGCGCGCTGGCGGGCGAAGAGGGCTTCGAGCTGGTTGATTTCCTGCTGGCTCTGCTG
CAGGTAGGCGATACCGGCGACCATCATCATCTGCAGGC
Blast against PA14
BLAST of Processed Sequence against PA14 And PAO1 Genome Sequence
104801 104801 104802 104802 104803 104803 104804 104804
PA14 BLAST Hit ID: 104801       Show Transposon Map
Identities: 348/350 (99.42%) BLAST Bit Score: 678.0 Percent of Processed Sequence That Aligns: 89.95%
Query Start: 25 Query End: 374 Subject Start: 425334 Subject End: 425683 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 425334 PA14_04800*,PA14_04810 -,-
Raw Sequence Start: 425274 PA14_04800*,PA14_04810 -,-
Extrapolated Insert Location: 425335 PA14_04800*,PA14_04810 -,-
Default Offset Location: 425337 PA14_04800*,PA14_04810 -,-
PA14 BLAST Hit ID: 104802       Show Transposon Map
Identities: 23/24 (95.83%) BLAST Bit Score: 40.1 Percent of Processed Sequence That Aligns: 5.93%
Query Start: 337 Query End: 360 Subject Start: 157140 Subject End: 157163 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 157140 PA14_01700*,PA14_01720 -,+
Raw Sequence Start: 156768 PA14_01700* -
Extrapolated Insert Location: 156829 PA14_01700* -
Default Offset Location: 156831 PA14_01700* -
PAO1 BLAST Hit ID: 104803       Show Transposon Map
Identities: 347/350 (99.14%) BLAST Bit Score: 670.0 Percent of Processed Sequence That Aligns: 89.95%
Query Start: 25 Query End: 374 Subject Start: 410585 Subject End: 410934 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 410585 PA0366 -
Raw Sequence Start: 410525 PA0366 -
Extrapolated Insert Location: 410586 PA0366 -
Default Offset Location: 410588 PA0366 -
PAO1 BLAST Hit ID: 104804       Show Transposon Map
Identities: 23/24 (95.83%) BLAST Bit Score: 40.1 Percent of Processed Sequence That Aligns: 5.93%
Query Start: 337 Query End: 360 Subject Start: 159175 Subject End: 159198 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 159175 PA0140 +
Raw Sequence Start: 158803
Extrapolated Insert Location: 158864
Default Offset Location: 158866
H PA14 Blast Hit H PAO1 Blast Hit