Mutant Report for Mutant ID: 56584
Strain: PA14 Genetic Background: Wild Type Transposon: MrT7 Library: lib_04_04
Raw Sequence ID: 31129
Chromat Path: H:\Pa14\MrT7\590v1\590v1_D04_D4_032.ab1 Last Transposon Base: Transposon Sequence
Match Quality:
Raw Sequence:
(Transposon Sequence in Red)		 
TTATCCGCCAACCTGTTACTACGGCCTGAAGGGCGGCCCGGTGCACGACGCCACGGTGGTCGCCTACCTG
CTCAAGCCCTCGCTGTTCAAGGGCAAGCGGATCAACGTGCAGGTCGACAGCCGCGAGGGCATCACCTTCG
GCCAGACCGTGGCCGACTGGTACGGCGGCCTCAAGCAGCCGGCCAACGTCGAGTGGATCAACGAGGGCGA
TGCCCAGGGGTTCTTCGACCTGCTCACCGAGCGTATCGCCCGCCTGCCCTGAGCGGGCTTCCCGCGGCGC
CCGGCGTGGGCGCCGCGCTCAGAACGCGTAGCCGACGTTGAAGTAGTGGCCGATCCCGCTGGAGTCGGTG
CGGCGTCCGTCCAGTTGCTCGCCGTCGCGCCACTGGGTCATGTTGCGGTAGACCTTGGCGCCGTAGCCGA
ACTTCCAGTGTTCGGTGTGATACCAGAGCCCCTCATCATCTGCTTGGCCTGGCCAAACTTTTTTACCGCC
CCCTTTTTCTCCCTTCTCTCCTCTTTTCAGTGTTGTGTTTCAAGTTTGCCCCCCCACCGCCCGAGTGTTC
TTCTTTGTTCCCTGTGATTCTCTGCCTTTTTACCCCTGCCCACCCTTTCATATTTTTTTCATTTCTTCCC
ACGTCTTCATTTCCCCTTCTCCCCCTCTTTCTTTGCCTTCCTCCCCCTTCTTGCTACGTTTTCCTTCCTA
CCCCCCCTCCCCCCTTTTATCCGCTCATTTTTTTCTTTTCTTTTTCTCTCTCTTTTCTTATTTTTTTCTC
TTCTGTTTTTTCTTTCTTCTCTCTCAGTTACACTCCCCCCTTCTTCCCTTCTTTTTACTCCTTTCTTCCC
CCCCTCGCTT
Blast against PA14
Processed Seqquence ID: 23934
5' Trim Position: 5 3' Trim Position: 404
Processed Sequence:
(Transposon Sequence in Red)		 
CGCCAACCTGTTACTACGGCCTGAAGGGCGGCCCGGTGCACGACGCCACGGTGGTCGCCTACCTGCTCAA
GCCCTCGCTGTTCAAGGGCAAGCGGATCAACGTGCAGGTCGACAGCCGCGAGGGCATCACCTTCGGCCAG
ACCGTGGCCGACTGGTACGGCGGCCTCAAGCAGCCGGCCAACGTCGAGTGGATCAACGAGGGCGATGCCC
AGGGGTTCTTCGACCTGCTCACCGAGCGTATCGCCCGCCTGCCCTGAGCGGGCTTCCCGCGGCGCCCGGC
GTGGGCGCCGCGCTCAGAACGCGTAGCCGACGTTGAAGTAGTGGCCGATCCCGCTGGAGTCGGTGCGGCG
TCCGTCCAGTTGCTCGCCGTCGCGCCACTGGGTCATGTTGCGGTAGACC
Blast against PA14
BLAST of Processed Sequence against PA14 And PAO1 Genome Sequence
104737 104737 104738 104738
PA14 BLAST Hit ID: 104737       Show Transposon Map
Identities: 388/388 (100.00%) BLAST Bit Score: 769.0 Percent of Processed Sequence That Aligns: 96.99%
Query Start: 12 Query End: 399 Subject Start: 162131 Subject End: 162518 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 162131 PA14_01760 +
Raw Sequence Start: 162115 PA14_01760 +
Extrapolated Insert Location:
Default Offset Location: 162178 PA14_01760 +
PAO1 BLAST Hit ID: 104738       Show Transposon Map
Identities: 247/247 (100.00%) BLAST Bit Score: 490.0 Percent of Processed Sequence That Aligns: 61.65%
Query Start: 12 Query End: 258 Subject Start: 164170 Subject End: 164416 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 164170 PA0143 +
Raw Sequence Start: 164154 PA0143 +
Extrapolated Insert Location:
Default Offset Location: 164217 PA0143 +
H PA14 Blast Hit H PAO1 Blast Hit