Mutant Report for Mutant ID: 56469
Strain: PA14 Genetic Background: Wild Type Transposon: MrT7 Library: lib_04_04
Raw Sequence ID: 31014
Chromat Path: H:\Pa14\MrT7\589v1\589v1_B09_B9_075.ab1 Last Transposon Base: 64 Transposon Sequence
Match Quality: 4
Raw Sequence:
(Transposon Sequence in Red)		 
TGTCAGTTACGAACCGACCAGGCATCATCTGCTGGCGTACCGCACACTTATCAGCCAACCTGATATAGGA
CCGCAACTACGCCCCCGCCGCCTTCATTCCTCTGCTGCGCAGCCTGATCACGCGTCTGGAACCAGAGCGG
CCGCGAACTGATCGATTTCGCCGGCGGCATCGCCGTGACCTCCCTGGGCCACGCGCATCCGGCGCTGGTC
AAGGCGCTCACCGAGCAGGCCCAGCGCATCTGGCACGTTTCCAACGTCTTCACCAACGAGCCGGCCCTGC
GCCTGGCGCGCAAGCTGGTGGACGCCACCTTCGCCGAGCGGGTGTTCCTCGCCAACTCCGGCGCCGAAGC
CAACGAGGCGGCCTTCAAGCTGGCTCGCCGCTACGCCAACGATGTCTACGGCCCGCAGAAGTACGAGATC
ATCGCGGCCAGCAACAGCTTCCACGGGCGCACCCTGTTCACCGTCAACGTCCGCGGCCAGCCGAAGTATT
CCGATGGCTTCGGGCCCAAGTTCGAGGGTATCACCCACGTTCCCTACAACGACCTGGAAGCCCTGGAAGG
GGCGATCTCCGACAAGACCTGCGCCGTGGTGCTGGAACCGATCCAGGGCGAGGGCGGCGTGCTGCCCGCT
CAACATGCCTACCTGGAAGGGGCGCGCCAGCTGCTGCACGAACACAATCGGCTGCTGGGCTTCGACGAGG
GTCAGAGCGGCATGGGCCGGGTCCGGCGAACTGTTCCCCTACATTCACTACTGGTGGACCCCGGAAATCC
TCCTCCGCCGCCAAAAACCTGGGCGGTGGCTTCCCTATTCGGGCAAAGGTGAACCCCCGGCGAAATCGCC
CAGGACCCTGCGGGTCTGCACCCCAGGGCACCCCTATTGGGGGTGACCCGCCTGACTTGGTGGATGG
Blast against PA14
Processed Seqquence ID: 23843
5' Trim Position: 77 3' Trim Position: 471
Processed Sequence:
(Transposon Sequence in Red)		 
TACGCCCCCGCCGCCTTCATTCCTCTGCTGCGCAGCCTGATCACGCGTCTGGAACCAGAGCGGCCGCGAA
CTGATCGATTTCGCCGGCGGCATCGCCGTGACCTCCCTGGGCCACGCGCATCCGGCGCTGGTCAAGGCGC
TCACCGAGCAGGCCCAGCGCATCTGGCACGTTTCCAACGTCTTCACCAACGAGCCGGCCCTGCGCCTGGC
GCGCAAGCTGGTGGACGCCACCTTCGCCGAGCGGGTGTTCCTCGCCAACTCCGGCGCCGAAGCCAACGAG
GCGGCCTTCAAGCTGGCTCGCCGCTACGCCAACGATGTCTACGGCCCGCAGAAGTACGAGATCATCGCGG
CCAGCAACAGCTTCCACGGGCGCACCCTGTTCACCGTCAACGTC
Blast against PA14
BLAST of Processed Sequence against PA14 And PAO1 Genome Sequence
104352 104352 104353 104353 104354 104354 104355 104355 104356 104356 104357 104357 104358 104358 104359 104359
PA14 BLAST Hit ID: 104352       Show Transposon Map
Identities: 354/356 (99.43%) BLAST Bit Score: 690.0 Percent of Processed Sequence That Aligns: 90.10%
Query Start: 39 Query End: 394 Subject Start: 4673584 Subject End: 4673229 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 4673584 PA14_52720,PA14_52710* +,-
Raw Sequence Start: 4673699 PA14_52710* -
Extrapolated Insert Location: 4673635 PA14_52720,PA14_52710* +,-
Default Offset Location: 4673636 PA14_52720,PA14_52710* +,-
PA14 BLAST Hit ID: 104353       Show Transposon Map
Identities: 26/27 (96.29%) BLAST Bit Score: 46.1 Percent of Processed Sequence That Aligns: 6.60%
Query Start: 74 Query End: 100 Subject Start: 315491 Subject End: 315517 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 315491 PA14_03440*,PA14_03450 -,+
Raw Sequence Start: 315341 PA14_03440* -
Extrapolated Insert Location: 315405 PA14_03440*,PA14_03450 -,+
Default Offset Location: 315404 PA14_03440*,PA14_03450 -,+
PA14 BLAST Hit ID: 104354       Show Transposon Map
Identities: 23/24 (95.83%) BLAST Bit Score: 40.1 Percent of Processed Sequence That Aligns: 5.84%
Query Start: 54 Query End: 77 Subject Start: 5067385 Subject End: 5067408 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 5067385 PA14_56880 +
Raw Sequence Start: 5067255 PA14_56880 +
Extrapolated Insert Location: 5067319 PA14_56880 +
Default Offset Location: 5067318 PA14_56880 +
PA14 BLAST Hit ID: 104355       Show Transposon Map
Identities: 20/20 (100.00%) BLAST Bit Score: 40.1 Percent of Processed Sequence That Aligns: 4.82%
Query Start: 81 Query End: 100 Subject Start: 5023736 Subject End: 5023717 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 5023736 PA14_56260,PA14_56270* +,+
Raw Sequence Start: 5023893 PA14_56280,PA14_56270* +,+
Extrapolated Insert Location: 5023829 PA14_56280,PA14_56270* +,+
Default Offset Location: 5023830 PA14_56280,PA14_56270* +,+
PAO1 BLAST Hit ID: 104356       Show Transposon Map
Identities: 351/356 (98.59%) BLAST Bit Score: 666.0 Percent of Processed Sequence That Aligns: 90.10%
Query Start: 39 Query End: 394 Subject Start: 978007 Subject End: 978362 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 978007 PA0895 +
Raw Sequence Start: 977892
Extrapolated Insert Location: 977956 PA0895 +
Default Offset Location: 977955 PA0895 +
PAO1 BLAST Hit ID: 104357       Show Transposon Map
Identities: 26/27 (96.29%) BLAST Bit Score: 46.1 Percent of Processed Sequence That Aligns: 6.60%
Query Start: 74 Query End: 100 Subject Start: 301350 Subject End: 301376 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 301350 PA0266 +
Raw Sequence Start: 301200
Extrapolated Insert Location: 301264 PA0266 +
Default Offset Location: 301263 PA0266 +
PAO1 BLAST Hit ID: 104358       Show Transposon Map
Identities: 23/24 (95.83%) BLAST Bit Score: 40.1 Percent of Processed Sequence That Aligns: 5.84%
Query Start: 54 Query End: 77 Subject Start: 4903832 Subject End: 4903855 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 4903832 PA4374 +
Raw Sequence Start: 4903702 PA4374 +
Extrapolated Insert Location: 4903766 PA4374 +
Default Offset Location: 4903765 PA4374 +
PAO1 BLAST Hit ID: 104359       Show Transposon Map
Identities: 20/20 (100.00%) BLAST Bit Score: 40.1 Percent of Processed Sequence That Aligns: 4.82%
Query Start: 81 Query End: 100 Subject Start: 4860184 Subject End: 4860165 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 4860184 PA4331 +
Raw Sequence Start: 4860341 PA4332 +
Extrapolated Insert Location: 4860277 PA4332 +
Default Offset Location: 4860278 PA4332 +
H PA14 Blast Hit H PAO1 Blast Hit