Mutant Report for Mutant ID: 48714
Strain: PA14 Genetic Background: Wild Type Transposon: MrT7 Library: lib_04_04
Raw Sequence ID: 37083
Chromat Path: H:\Pa14\MrT7\508v1\508v1_D06_D6_047.ab1 Last Transposon Base: 63 Transposon Sequence
Match Quality: 0
Raw Sequence:
(Transposon Sequence in Red)		 
GTTCAGTTTGTATACGACTCACTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTAGGCATC
GGCGCCCTGGAAGAAGTCCACGTCGGTGAGCACCGACAGGCACGCCGCGCCACCCGCCTCGTAGCTGCGG
GCGATCTCCGCCGGGACGAAGTGTTCGCGCAGCACGCCCTTGCTCGGCGATGCCTTCTTGATCTCGGCGA
TCACTGCCGGCTCCTTGCGCTTGGCCCGCTCCAGCAGGGCATTGGCGAAGCCGCGCGGCGCATCGGCGCT
ACGCGCCAGCCGCTCGACCTCGGCCAGGTTGACGCGCGCTCGGCGCTCGGCGACCTCCTCGGCCTTGCGG
GCGAGGATCTTCTGCAGAACCGTCGGCACACTCACTGTGCGTTCTCCTCTCTGTAAACGGCGGTGAAGGC
CACCAGTTCGTCCATCTTCTCCCGTGCCAGCCCGGTGTGCAGGGCATCGTGGGCCAGTTGAATGCCCTCG
TGCAGGCTGGTCGCCAGATCGGCAGCGTACAGTGCCGGGCCGGCATTCATCACGATCAGCTCGGCGGCCT
TCTGCCCAGCCTCGGTCTTGCGCCGCCCCAAAGCGTCGCGGATCAGTTCCAGCGAGGCCTGCGGACTGTC
GACCTCCAGCCCCATCAGGGTCTGGCTCTTGATCCCGAAGTCCTCGGGACGCACTTCGTACTCGCGTACC
TCGCCGGCTCATCATCGGGAAGGGCCTGGGCCAAATNTNTNTTTTGTTANGAATCATCCTTTTCTTCTCC
ATCATTTGCTCCCATCTATTCCATGTCATCTTACCCGATCAAACTGCTATTGTTAACTTATACCAACTAT
TCTATTTATATTGCTTGCTCTATTCTATC
Blast against PA14
Processed Seqquence ID: 28304
5' Trim Position: 4 3' Trim Position: 701
Processed Sequence:
(Transposon Sequence in Red)		 
AGTTTGTATACGACTCACTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTAGGCATCGGCG
CCCTGGAAGAAGTCCACGTCGGTGAGCACCGACAGGCACGCCGCGCCACCCGCCTCGTAGCTGCGGGCGA
TCTCCGCCGGGACGAAGTGTTCGCGCAGCACGCCCTTGCTCGGCGATGCCTTCTTGATCTCGGCGATCAC
TGCCGGCTCCTTGCGCTTGGCCCGCTCCAGCAGGGCATTGGCGAAGCCGCGCGGCGCATCGGCGCTACGC
GCCAGCCGCTCGACCTCGGCCAGGTTGACGCGCGCTCGGCGCTCGGCGACCTCCTCGGCCTTGCGGGCGA
GGATCTTCTGCAGAACCGTCGGCACACTCACTGTGCGTTCTCCTCTCTGTAAACGGCGGTGAAGGCCACC
AGTTCGTCCATCTTCTCCCGTGCCAGCCCGGTGTGCAGGGCATCGTGGGCCAGTTGAATGCCCTCGTGCA
GGCTGGTCGCCAGATCGGCAGCGTACAGTGCCGGGCCGGCATTCATCACGATCAGCTCGGCGGCCTTCTG
CCCAGCCTCGGTCTTGCGCCGCCCCAAAGCGTCGCGGATCAGTTCCAGCGAGGCCTGCGGACTGTCGACC
TCCAGCCCCATCAGGGTCTGGCTCTTGATCCCGAAGTCCTCGGGACGCACTTCGTACTCGCGTACCT
Blast against PA14
BLAST of Processed Sequence against PA14 And PAO1 Genome Sequence
120049 120049 120050 120050 120051 120051 120052 120052 120053 120053
PA14 BLAST Hit ID: 120049       Show Transposon Map
Identities: 639/639 (100.00%) BLAST Bit Score: 999.9 Percent of Processed Sequence That Aligns: 91.54%
Query Start: 59 Query End: 697 Subject Start: 716436 Subject End: 715798 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 716436 PA14_08360 -
Raw Sequence Start: 716498 PA14_08360 -
Extrapolated Insert Location: 716435 PA14_08360 -
Default Offset Location: 716435 PA14_08360 -
PA14 BLAST Hit ID: 120050       Show Transposon Map
Identities: 22/22 (100.00%) BLAST Bit Score: 44.1 Percent of Processed Sequence That Aligns: 3.01%
Query Start: 594 Query End: 615 Subject Start: 4288340 Subject End: 4288319 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 4288340 PA14_48160 -
Raw Sequence Start: 4288937 PA14_48160 -
Extrapolated Insert Location: 4288874 PA14_48160 -
Default Offset Location: 4288874 PA14_48160 -
PA14 BLAST Hit ID: 120051       Show Transposon Map
Identities: 20/20 (100.00%) BLAST Bit Score: 40.1 Percent of Processed Sequence That Aligns: 2.73%
Query Start: 133 Query End: 152 Subject Start: 1318624 Subject End: 1318605 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 1318624 PA14_15580 -
Raw Sequence Start: 1318760 PA14_15580 -
Extrapolated Insert Location: 1318697 PA14_15580 -
Default Offset Location: 1318697 PA14_15580 -
PAO1 BLAST Hit ID: 120052       Show Transposon Map
Identities: 637/639 (99.68%) BLAST Bit Score: 999.9 Percent of Processed Sequence That Aligns: 91.54%
Query Start: 59 Query End: 697 Subject Start: 705452 Subject End: 704814 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 705452 PA0651 -
Raw Sequence Start: 705514 PA0651 -
Extrapolated Insert Location: 705451 PA0651 -
Default Offset Location: 705451 PA0651 -
PAO1 BLAST Hit ID: 120053       Show Transposon Map
Identities: 22/22 (100.00%) BLAST Bit Score: 44.1 Percent of Processed Sequence That Aligns: 3.01%
Query Start: 594 Query End: 615 Subject Start: 1346256 Subject End: 1346277 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 1346256 PA1243 -
Raw Sequence Start: 1345659 PA1243 -
Extrapolated Insert Location: 1345722 PA1243 -
Default Offset Location: 1345722 PA1243 -
H PA14 Blast Hit H PAO1 Blast Hit