Mutant Report for Mutant ID: 43390
Strain: PA14 Genetic Background: Wild Type Transposon: MrT7 Library: lib_04_04
Raw Sequence ID: 27151
Chromat Path: H:\Pa14\MrT7\452v1\452v1_H10_H10_090.ab1 Last Transposon Base: 120 Transposon Sequence
Match Quality: 6
Raw Sequence:
(Transposon Sequence in Red)		 
TTTTTTTTTTTTTTTTNTTTTTTTTTTTTTTCTTTTTTTNNNNTNNTTNCNTTTNNNNATTGTTCAGTTT
GTATACGACTCACTATAGGGGTCTAGAGACCGGGGCACTTATCAGCCAACCTGTTACAACAAGGTCGCGC
AGCTCACCGTAGGAGGAGAAGGCCCGCGGGGTGATCGCCGCCTCGGCCGGCAACCACGCCCAGGGCCTGG
CCCTGGCGGCCAAGCGGCAGGGGATCAGGGCGGTCATCGTGATGCCCAAGACCACCCCCGAGATCAAGGT
CCAGGCGGTGCGCGCCCACGGCGCCAAGGCGGTGCTGCACGGCGACGCCTTCCCCGAGGCGCTGGCCCAC
GCGCTGAAGCTGGTCGACGAGAAGGGCTACACCTTCGTCCATCCATACTACTATCCTTCATCATCTGCAG
GCCTGGCCATTTTTTTTTTTTTTTTTTTTTCTCTCTCTTTCTCCCCTCTCCTTCCCCCCTTTTTCATTCC
TCTCTTTCTCTTTTTTTTCTCCCCCCCTCCTTTCATTCTTCTTTGCTTGTCGTACTTTTATTTTCCTCTA
CCCATTTTTTTTTTACGCCCCACCCTCCTCCCGTCCTTTTTCCTCCTCCCCCTTGTTTACTCTTGTCTCT
TGTTTGACTTCGTTGTCCTCCACCCCCACTCCATATTTTCTGCCGACGCATTTTTTCTCCTCCCTAATTT
TCCTTTCTTCGTGCGTCCTTCGTTTTCTTTCGTTTTCTTTCCCCTCCTTTTATTCTCTCTTCTTGTTCTT
ATTCCCGCCCGCTCCACTATAGTAGGACGCGCTCTGCCGTACTAATTTCCTTGTAGTCTTTTGTGTCTGT
CTCTTATTTTTTTTATTTTCTCTTATTTTTTCTTTTATTGCCCCTAGTGTCTCTTTTCATAGGATATATT
TATCTATTAATTGCTATGTTTTGACTTATGACTACCCTTCTTACTTTCGCCTACTTT
Blast against PA14
Processed Seqquence ID: 20740
5' Trim Position: 17 3' Trim Position: 427
Processed Sequence:
(Transposon Sequence in Red)		 
TTTTTTTTTTTTTTCTTTTTTTNNNNTNNTTNCNTTTNNNNATTGTTCAGTTTGTATACGACTCACTATA
GGGGTCTAGAGACCGGGGCACTTATCAGCCAACCTGTTACAACAAGGTCGCGCAGCTCACCGTAGGAGGA
GAAGGCCCGCGGGGTGATCGCCGCCTCGGCCGGCAACCACGCCCAGGGCCTGGCCCTGGCGGCCAAGCGG
CAGGGGATCAGGGCGGTCATCGTGATGCCCAAGACCACCCCCGAGATCAAGGTCCAGGCGGTGCGCGCCC
ACGGCGCCAAGGCGGTGCTGCACGGCGACGCCTTCCCCGAGGCGCTGGCCCACGCGCTGAAGCTGGTCGA
CGAGAAGGGCTACACCTTCGTCCATCCATACTACTATCCTTCATCATCTGCAGGCCTGGC
Blast against PA14
BLAST of Processed Sequence against PA14 And PAO1 Genome Sequence
93460 93460 93461 93461 93462 93462 93463 93463 93464 93464 93465 93465
PA14 BLAST Hit ID: 93460       Show Transposon Map
Identities: 269/270 (99.62%) BLAST Bit Score: 519.0 Percent of Processed Sequence That Aligns: 65.61%
Query Start: 108 Query End: 377 Subject Start: 386425 Subject End: 386693 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 386425 PA14_04320 +
Raw Sequence Start: 386301 PA14_04320 +
Extrapolated Insert Location: 386421 PA14_04320 +
Default Offset Location: 386364 PA14_04320 +
PA14 BLAST Hit ID: 93461       Show Transposon Map
Identities: 163/193 (84.45%) BLAST Bit Score: 145.0 Percent of Processed Sequence That Aligns: 46.83%
Query Start: 140 Query End: 332 Subject Start: 4197337 Subject End: 4197145 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 4197337 PA14_47100 +
Raw Sequence Start: 4197493 PA14_47100 +
Extrapolated Insert Location: 4197373 PA14_47100 +
Default Offset Location: 4197430 PA14_47100 +
PA14 BLAST Hit ID: 93462       Show Transposon Map
Identities: 20/20 (100.00%) BLAST Bit Score: 40.1 Percent of Processed Sequence That Aligns: 4.63%
Query Start: 188 Query End: 207 Subject Start: 2959844 Subject End: 2959863 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 2959844 PA14_33610 +
Raw Sequence Start: 2959640 PA14_33610 +
Extrapolated Insert Location: 2959760 PA14_33610 +
Default Offset Location: 2959703 PA14_33610 +
PAO1 BLAST Hit ID: 93463       Show Transposon Map
Identities: 269/270 (99.62%) BLAST Bit Score: 519.0 Percent of Processed Sequence That Aligns: 65.61%
Query Start: 108 Query End: 377 Subject Start: 372256 Subject End: 372524 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 372256 PA0331 +
Raw Sequence Start: 372132 PA0331 +
Extrapolated Insert Location: 372252 PA0331 +
Default Offset Location: 372195 PA0331 +
PAO1 BLAST Hit ID: 93464       Show Transposon Map
Identities: 163/193 (84.45%) BLAST Bit Score: 145.0 Percent of Processed Sequence That Aligns: 46.83%
Query Start: 140 Query End: 332 Subject Start: 1437296 Subject End: 1437488 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 1437296 PA1326 +
Raw Sequence Start: 1437140 PA1326 +
Extrapolated Insert Location: 1437260 PA1326 +
Default Offset Location: 1437203 PA1326 +
PAO1 BLAST Hit ID: 93465       Show Transposon Map
Identities: 20/20 (100.00%) BLAST Bit Score: 40.1 Percent of Processed Sequence That Aligns: 4.63%
Query Start: 188 Query End: 207 Subject Start: 2683948 Subject End: 2683929 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 2683948 PA2402 +
Raw Sequence Start: 2684152 PA2402 +
Extrapolated Insert Location: 2684032 PA2402 +
Default Offset Location: 2684089 PA2402 +
H PA14 Blast Hit H PAO1 Blast Hit