Mutant Report for Mutant ID: 40395
Strain: PA14 Genetic Background: Wild Type Transposon: MrT7 Library: lib_04_04
Raw Sequence ID: 24156
Chromat Path: H:\Pa14\MrT7\421v1\421v1_G03_G3_021.ab1 Last Transposon Base: Transposon Sequence
Match Quality:
Raw Sequence:
(Transposon Sequence in Red)		 
ATCTATACATATATCTCTTTCTTTTTATTTAAGTTTGATTTGTTTTTATATTTTATCTTCCTTNNNNNNN
GTGTTCAGTTTGTAATACGACTACAGTATCATCTGCTGGAGTCCGGAGAACTTATCAGCCAACCTGTTAA
TCCATCGAGGGTTCCTTGTAACGAATCGGCGTGTCGGTCGGCGGAGTTTACCTGCCTGCCGGCACGGATG
GGCGTGCATCCGTCGTCACGGCAGGAAATGTTGCAGCAGGTCGTTGAGGAACAGCTGGCCGCGTTCGCTG
GGACGCAGGCGCTGCGGGTCGTCGGCGAGCAGCCCGGCGTCGCGCGCGGCGGCGCAGGCATCGGCGATCG
CCGCCAGCGGTTGGCCGGTGCGCTGCGGGTAGAGCGCGGCGGGGACGCCGTCGACCAGTCGCAGCACGTT
CATCATGAATTCGAATGGCAGGTCGGCCGCTTCCAGCGGGCGCTCGCCGGCCTGGAAGGCCTTTTGCGGG
TTGAGGTAGTCCTTCAGCAGGCGGGTCTTCCAGGTCCGCACGATGCGTCCGTCCCGGCGCGCTCAACTTG
GCGTGGGCGCCGGCGCCTATGCCGAGGAAGTCGCCGAAGCTCCAGTAGTTCAGGTTGTGCCGGGCGCGCA
GGTCTTCCCCGTGCGTAAGGCGAAGGTCTCCTTACTGGCGGGTAACCATTATACGTCAAGGCTGGGCCAG
GCACATATTAATACTTAAAAAAACTAAAACATAAATATCCTTCCACTAGTTGCAGCATCTCTCGCACAGT
CAAATTTAAAAAAAAATCTCCCACTTTTTAACCATCCTATTAAATTGCTAACATCTAACTAATTCAAAAT
TTCACAAACATGACACAATAACTAAGAAACTAAAGGAAACACTAGTACTGTATAATATTACAAATTATTA
CCTTATCACCGTTCATAATGTCGAGACCAAGATTATATGCGTGTATTCAGATATGGACGTTCGAATTCAT
ACCATCACTAACCGATAGAATCCGAATATAAGAGACAAATAAACACTATTAAACTGCCAAATCTGATTCC
AATTTATAT
Blast against PA14
Processed Seqquence ID: 18250
5' Trim Position: 117 3' Trim Position: 538
Processed Sequence:
(Transposon Sequence in Red)		 
GAACTTATCAGCCAACCTGTTAATCCATCGAGGGTTCCTTGTAACGAATCGGCGTGTCGGTCGGCGGAGT
TTACCTGCCTGCCGGCACGGATGGGCGTGCATCCGTCGTCACGGCAGGAAATGTTGCAGCAGGTCGTTGA
GGAACAGCTGGCCGCGTTCGCTGGGACGCAGGCGCTGCGGGTCGTCGGCGAGCAGCCCGGCGTCGCGCGC
GGCGGCGCAGGCATCGGCGATCGCCGCCAGCGGTTGGCCGGTGCGCTGCGGGTAGAGCGCGGCGGGGACG
CCGTCGACCAGTCGCAGCACGTTCATCATGAATTCGAATGGCAGGTCGGCCGCTTCCAGCGGGCGCTCGC
CGGCCTGGAAGGCCTTTTGCGGGTTGAGGTAGTCCTTCAGCAGGCGGGTCTTCCAGGTCCGCACGATGCG
T
Blast against PA14
BLAST of Processed Sequence against PA14 And PAO1 Genome Sequence
83006 83006 83007 83007 83008 83008 83009 83009 83010 83010 83011 83011
PA14 BLAST Hit ID: 83006       Show Transposon Map
Identities: 400/401 (99.75%) BLAST Bit Score: 787.0 Percent of Processed Sequence That Aligns: 95.01%
Query Start: 21 Query End: 421 Subject Start: 442234 Subject End: 442634 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 442234 PA14_05030 -
Raw Sequence Start: 442097 PA14_05030 -
Extrapolated Insert Location:
Default Offset Location: 442160 PA14_05030 -
PA14 BLAST Hit ID: 83007       Show Transposon Map
Identities: 27/29 (93.10%) BLAST Bit Score: 42.1 Percent of Processed Sequence That Aligns: 6.65%
Query Start: 168 Query End: 196 Subject Start: 3793272 Subject End: 3793244 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 3793272 PA14_42650*,PA14_42640 -,-
Raw Sequence Start: 3793556 PA14_42650*,PA14_42660 -,-
Extrapolated Insert Location:
Default Offset Location: 3793493 PA14_42650*,PA14_42660 -,-
PA14 BLAST Hit ID: 83008       Show Transposon Map
Identities: 21/21 (100.00%) BLAST Bit Score: 42.1 Percent of Processed Sequence That Aligns: 4.75%
Query Start: 254 Query End: 274 Subject Start: 1953122 Subject End: 1953102 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 1953122 PA14_22430*,PA14_22440 -,+
Raw Sequence Start: 1953492 PA14_22430*,PA14_22440 -,+
Extrapolated Insert Location:
Default Offset Location: 1953429 PA14_22430*,PA14_22440 -,+
PAO1 BLAST Hit ID: 83009       Show Transposon Map
Identities: 395/401 (98.50%) BLAST Bit Score: 747.0 Percent of Processed Sequence That Aligns: 95.01%
Query Start: 21 Query End: 421 Subject Start: 427498 Subject End: 427898 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 427498 PA0385 -
Raw Sequence Start: 427361 PA0385 -
Extrapolated Insert Location:
Default Offset Location: 427424 PA0385 -
PAO1 BLAST Hit ID: 83010       Show Transposon Map
Identities: 21/21 (100.00%) BLAST Bit Score: 42.1 Percent of Processed Sequence That Aligns: 4.75%
Query Start: 254 Query End: 274 Subject Start: 3615989 Subject End: 3616009 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 3615989 PA3228 +
Raw Sequence Start: 3615619 PA3228 +
Extrapolated Insert Location:
Default Offset Location: 3615682 PA3228 +
PAO1 BLAST Hit ID: 83011       Show Transposon Map
Identities: 27/29 (93.10%) BLAST Bit Score: 42.1 Percent of Processed Sequence That Aligns: 6.65%
Query Start: 168 Query End: 196 Subject Start: 1841540 Subject End: 1841568 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 1841540 PA1691 -
Raw Sequence Start: 1841256 PA1690 -
Extrapolated Insert Location:
Default Offset Location: 1841319 PA1690 -
H PA14 Blast Hit H PAO1 Blast Hit