Mutant Report for Mutant ID: 40094
Strain: PA14 Genetic Background: Wild Type Transposon: MrT7 Library: lib_04_04
Raw Sequence ID: 23855
Chromat Path: H:\Pa14\MrT7\418v1\418v1_F02_F2_016.ab1 Last Transposon Base: 62 Transposon Sequence
Match Quality: 0
Raw Sequence:
(Transposon Sequence in Red)		 
TGTTCAGTTTGAATCGACTCCTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTACCTGGGC
GAAACCGCTGGGGACTGCCCGCTGCTCGGCGGCGTCGCGGTGTCGGTGCCGTTCCGCCTGGACGAGTGCG
CCGACCGCATCGGCCTGGGCTTTTCCCGGGTCTACCAGGCGCACTTCATGAAGGCCATGCTGGCCTACGT
GCAGGACAAGCAGCGGCTGTTCGGCGAGCAGGGCCAGGCGGAAGGCCTGGCCGCCCTGCAACGCCTCGGT
CCGCTGGAGGGCATGCGCACCTTCTGGGACTTCGACGGCCGGGTCACCGCGCCGCTGCACGGCTTCGCCG
ACGCCAGGGATTACTATCGGCGCGCGTCCAGCCGCTACTACCTGCCGGACATACGCACGCCGAGCCTGAT
CTGCCAGTTCTGGCCACGACCCCTTCGTGTTCGCCCGCAGACTGCCCGATCGCAACGAACTGGCACCCTG
CACCGAGCTGGAACTGCACGCCCGCGGGGGTCACGTCGGCTTCTTCGACGGCTCTCCGCGCCAGCCCACC
TACACTTGGAACAACGCACCCCCGAATGGTTGGAGCCCCGCCTGCCGCAGCACTGAACTGGCCGGCATCC
GGCGAAGGGCGGCAAAAAAGGAAAGGCTGAAATGAAAAAGGGGGCCAAAGGGAAATGAACCTTGCGGGCA
CAACCCCATATGCTCGAACAACCCCTAATGGCGAAGGGGAATCCCTGGGGAAACCCCCCCGGCGGATTCC
GCTTGGTAACCCGTCGCAATGGCCCCCACTGTCCGAGATTAACGCAAACCTGTTAACAATAATATTCCTG
TGTTTATTTCCCTTGGTTGGCCCTAAAACCGGTAAAAGTCACCTACAGGTCAGCGATCGTGGTTTACACA
CCCAAAAGAAA
Blast against PA14
Processed Seqquence ID: 17962
5' Trim Position: 14 3' Trim Position: 476
Processed Sequence:
(Transposon Sequence in Red)		 
CGACTCCTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTACCTGGGCGAAACCGCTGGGGA
CTGCCCGCTGCTCGGCGGCGTCGCGGTGTCGGTGCCGTTCCGCCTGGACGAGTGCGCCGACCGCATCGGC
CTGGGCTTTTCCCGGGTCTACCAGGCGCACTTCATGAAGGCCATGCTGGCCTACGTGCAGGACAAGCAGC
GGCTGTTCGGCGAGCAGGGCCAGGCGGAAGGCCTGGCCGCCCTGCAACGCCTCGGTCCGCTGGAGGGCAT
GCGCACCTTCTGGGACTTCGACGGCCGGGTCACCGCGCCGCTGCACGGCTTCGCCGACGCCAGGGATTAC
TATCGGCGCGCGTCCAGCCGCTACTACCTGCCGGACATACGCACGCCGAGCCTGATCTGCCAGTTCTGGC
CACGACCCCTTCGTGTTCGCCCGCAGACTGCCCGATCGCAAC
Blast against PA14
BLAST of Processed Sequence against PA14 And PAO1 Genome Sequence
81637 81637 81638 81638
PA14 BLAST Hit ID: 81637       Show Transposon Map
Identities: 405/413 (98.06%) BLAST Bit Score: 747.0 Percent of Processed Sequence That Aligns: 89.18%
Query Start: 48 Query End: 460 Subject Start: 427631 Subject End: 427220 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 427631 PA14_04840 +
Raw Sequence Start: 427692 PA14_04840 +
Extrapolated Insert Location: 427630 PA14_04840 +
Default Offset Location: 427629 PA14_04840 +
PAO1 BLAST Hit ID: 81638       Show Transposon Map
Identities: 405/413 (98.06%) BLAST Bit Score: 747.0 Percent of Processed Sequence That Aligns: 89.18%
Query Start: 48 Query End: 460 Subject Start: 412883 Subject End: 412472 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 412883 PA0368 +
Raw Sequence Start: 412944 PA0368 +
Extrapolated Insert Location: 412882 PA0368 +
Default Offset Location: 412881 PA0368 +
H PA14 Blast Hit H PAO1 Blast Hit