Mutant Report for Mutant ID: 39946
Strain: PA14 Genetic Background: Wild Type Transposon: MrT7 Library: lib_04_04
Raw Sequence ID: 23707
Chromat Path: H:\Pa14\MrT7\417v1\417v1_A10_A10_071.ab1 Last Transposon Base: 64 Transposon Sequence
Match Quality: 1
Raw Sequence:
(Transposon Sequence in Red)		 
TGTTCAGTTTGTATACGACTCACTATAGTATGCTAGAGACCGGGGACTTATCAGCCAACCTGATAAAGAC
CTGCTCGCCAGCGCGACGAACTCCAGGCCTAGATCGACGGCTGGCCACAGGCTCGCGCCGGCCAGGCCCA
CGACGCCGCTGCCTACAAGGCCTTCCTCGAGGAAATCGGCTACCTGCTGCCCGAGGCGGAAGACTTCCAG
GCCGGCACCCAGAACGTCGACGACGAGATCGCCCGCATGGCCGGCCCGCAGCTGGTGGTGCCGGTGATGA
ATGCGCGCTTCGCCCTGAACGCCTCCAACGCCCGCTGGGGCTCGCTGTACGACGCACTCTACGGCACCGA
CGTGATCAGCGAAGAAGGCGGCGCCGAGAAAGGCAAGGGCTACAACAAGGTTCGCGGCGACAAGGTCATC
GCCTTCGCTCGCGCCTTCCTCGACGAAGCCGCGCCGCTGGAGTCCGGCTCCCATGTCGACGCCACTTCCT
ACAGCGTGAAGAACGGCGCGCTGGTCGTCGCGCTGAAGAACGGCAGCGAAACCGGCCTGAAGAACGCTGG
CCAGTTCCTTGCCTTCCAGGGCGACGCCGCCAAGCCGCAAGCGATACTGCTCAAGCACATCATCTGGAGG
GCCTGGAAACCCAAATCGACCCGTAGATGCCCGGGTTCGGCCAATACCATTGCCATCAGGACGAGAAAGA
ACGAGGCTGATTGGAAGCCCGCCCTCACCCCCCATTCATTTCTATGGTCTGGTGCACAGACCCCCGCTGG
CCGAGACCGACGGAGATAGCCGGGGGGTCCTCTCTCGAATTGGGTCGGGCCTGTTGAAAGAGCCACCAGG
CCGCGAGGTATGCAGTTAGGGCGGGGACGCACCTTTTCCCTGTCTCTCTTTTTCTCGCC
Blast against PA14
Processed Seqquence ID: 17827
5' Trim Position: 109 3' Trim Position: 610
Processed Sequence:
(Transposon Sequence in Red)		 
GCTGGCCACAGGCTCGCGCCGGCCAGGCCCACGACGCCGCTGCCTACAAGGCCTTCCTCGAGGAAATCGG
CTACCTGCTGCCCGAGGCGGAAGACTTCCAGGCCGGCACCCAGAACGTCGACGACGAGATCGCCCGCATG
GCCGGCCCGCAGCTGGTGGTGCCGGTGATGAATGCGCGCTTCGCCCTGAACGCCTCCAACGCCCGCTGGG
GCTCGCTGTACGACGCACTCTACGGCACCGACGTGATCAGCGAAGAAGGCGGCGCCGAGAAAGGCAAGGG
CTACAACAAGGTTCGCGGCGACAAGGTCATCGCCTTCGCTCGCGCCTTCCTCGACGAAGCCGCGCCGCTG
GAGTCCGGCTCCCATGTCGACGCCACTTCCTACAGCGTGAAGAACGGCGCGCTGGTCGTCGCGCTGAAGA
ACGGCAGCGAAACCGGCCTGAAGAACGCTGGCCAGTTCCTTGCCTTCCAGGGCGACGCCGCCAAGCCGCA
AGCGATACTGC
Blast against PA14
BLAST of Processed Sequence against PA14 And PAO1 Genome Sequence
80899 80899 80900 80900
PA14 BLAST Hit ID: 80899       Show Transposon Map
Identities: 491/493 (99.59%) BLAST Bit Score: 961.0 Percent of Processed Sequence That Aligns: 98.20%
Query Start: 9 Query End: 501 Subject Start: 556931 Subject End: 556439 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 556931 PA14_06290 +
Raw Sequence Start: 557048 PA14_06290 +
Extrapolated Insert Location: 556984 PA14_06290 +
Default Offset Location: 556985 PA14_06290 +
PAO1 BLAST Hit ID: 80900       Show Transposon Map
Identities: 487/493 (98.78%) BLAST Bit Score: 930.0 Percent of Processed Sequence That Aligns: 98.20%
Query Start: 9 Query End: 501 Subject Start: 544143 Subject End: 543651 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 544143 PA0482 +
Raw Sequence Start: 544260 PA0482 +
Extrapolated Insert Location: 544196 PA0482 +
Default Offset Location: 544197 PA0482 +
H PA14 Blast Hit H PAO1 Blast Hit