Mutant Report for Mutant ID: 35448
Strain: PA14 Genetic Background: Wild Type Transposon: MrT7 Library: lib_04_04
Raw Sequence ID: 19209
Chromat Path: H:\Pa14\MrT7\370v1\370v1_B12_B12_101.ab1 Last Transposon Base: 25 Transposon Sequence
Match Quality: 1
Raw Sequence:
(Transposon Sequence in Red)		 
CCGGGGACTTATCAGCCAACCTGTTAGTAATCCAGGGCCAGCGCTTGCAGGGTGGGGCAGGCCGCCAGGC
CGCCATCGGCGCACTGGCTGACACCGTCGGCGATGGCCAGCAGGTGTCCCTTGCTGGCGGCCAGGGCCGG
CGCCGGGGTGACTACCCGCAGCGCGTCCTGGTTTTCCGTGCGAGGCCCGCAGGCGCTGGCCTCGCCGATG
CTCAGTCGCAGCCCCACGCTCAGACCCGCGCGGCGGTGACCGCGGCCGAGCCCCAGGTGGTGCGCCAGCG
CCGCTTCACCCCGTGGAGGCCGAACCAGGCCAGCACGCCGAGACTGGCGAACAGCCACAGGCCGAGCTGG
TAGACGCCGGTGCTTTGCTTGATCGCGCCAAGGTCGACTGTCAGGCATAATCCGCCGATGCCGCCGGTCA
TGCCGATCAGCCCGGTCATCACGCCGATTTCCTTGTGGAATCGCTGCGTTACCAACTGGAACACCGCGCC
ATTGCCGGCACCGATACTGATCATGCTGACCACCAACAGAGCTAGATCGGCCATCGCCCTGGGTAATTGG
AAACCGACCGCGGCGATGTACATGTTGGTCAGGGTGTCCACCATCATTATGCTGTCGATGCCTCCCATGC
TATCTGCTAGATCGCCCACAATTGTCGCTTCCAGGCTTCCGGTCAAAAACCAAGTGGCCGCGTAATATCC
CGGCGTCACCAGGTTCAGGCCCTAATGGATTTGGAAATTAACAGTCCGAGCTTAGCATACTCCTGTAACA
AAAGCAATAAATCGTTTTTATACCTAATTTCTCCTTTTAATAACATCACCAAAACTTATTTTACTTTATC
CTGTTATTCATCAAA
Blast against PA14
Processed Seqquence ID: 13987
5' Trim Position: 1 3' Trim Position: 460
Processed Sequence:
(Transposon Sequence in Red)		 
CGGGGACTTATCAGCCAACCTGTTAGTAATCCAGGGCCAGCGCTTGCAGGGTGGGGCAGGCCGCCAGGCC
GCCATCGGCGCACTGGCTGACACCGTCGGCGATGGCCAGCAGGTGTCCCTTGCTGGCGGCCAGGGCCGGC
GCCGGGGTGACTACCCGCAGCGCGTCCTGGTTTTCCGTGCGAGGCCCGCAGGCGCTGGCCTCGCCGATGC
TCAGTCGCAGCCCCACGCTCAGACCCGCGCGGCGGTGACCGCGGCCGAGCCCCAGGTGGTGCGCCAGCGC
CGCTTCACCCCGTGGAGGCCGAACCAGGCCAGCACGCCGAGACTGGCGAACAGCCACAGGCCGAGCTGGT
AGACGCCGGTGCTTTGCTTGATCGCGCCAAGGTCGACTGTCAGGCATAATCCGCCGATGCCGCCGGTCAT
GCCGATCAGCCCGGTCATCACGCCGATTTCCTTGTGGAA
Blast against PA14
BLAST of Processed Sequence against PA14 And PAO1 Genome Sequence
62840 62840 62841 62841 62842 62842 62843 62843
PA14 BLAST Hit ID: 62840       Show Transposon Map
Identities: 423/436 (97.01%) BLAST Bit Score: 761.0 Percent of Processed Sequence That Aligns: 94.77%
Query Start: 24 Query End: 459 Subject Start: 3704314 Subject End: 3703879 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 3704314 PA14_41520 -
Raw Sequence Start: 3704338 PA14_41520 -
Extrapolated Insert Location: 3704313 PA14_41520 -
Default Offset Location: 3704275 PA14_41520 -
PA14 BLAST Hit ID: 62841       Show Transposon Map
Identities: 21/21 (100.00%) BLAST Bit Score: 42.1 Percent of Processed Sequence That Aligns: 4.36%
Query Start: 57 Query End: 77 Subject Start: 4231154 Subject End: 4231134 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 4231154 PA14_47500 -
Raw Sequence Start: 4231211 PA14_47500 -
Extrapolated Insert Location: 4231186 PA14_47500 -
Default Offset Location: 4231148 PA14_47500 -
PAO1 BLAST Hit ID: 62842       Show Transposon Map
Identities: 419/436 (96.10%) BLAST Bit Score: 730.0 Percent of Processed Sequence That Aligns: 94.77%
Query Start: 24 Query End: 459 Subject Start: 1930359 Subject End: 1930794 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 1930359 PA1782 -
Raw Sequence Start: 1930335 PA1782 -
Extrapolated Insert Location: 1930360 PA1782 -
Default Offset Location: 1930398 PA1782 -
PAO1 BLAST Hit ID: 62843       Show Transposon Map
Identities: 21/21 (100.00%) BLAST Bit Score: 42.1 Percent of Processed Sequence That Aligns: 4.36%
Query Start: 57 Query End: 77 Subject Start: 1403450 Subject End: 1403470 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 1403450 PA1292 -
Raw Sequence Start: 1403393 PA1292 -
Extrapolated Insert Location: 1403418 PA1292 -
Default Offset Location: 1403456 PA1292 -
H PA14 Blast Hit H PAO1 Blast Hit