Mutant Report for Mutant ID: 33288
Strain: PA14 Genetic Background: Wild Type Transposon: MrT7 Library: lib_04_04
Raw Sequence ID: 17064
Chromat Path: H:\Pa14\MrT7\347v1\347v1_F12_F12_101.ab1 Last Transposon Base: 62 Transposon Sequence
Match Quality: 0
Raw Sequence:
(Transposon Sequence in Red)		 
TGTCGTTTGTATACGACTCACTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTAGTGCTCC
ACCACCACGTTCTTCAGTTCCTCGGGTAGGCGATTTACCAGGCGATCGACCTCCGCCATACGGTCGAGGA
TCATCCTGTGGCCGCCTGGGCAGCGGACCACCTCGCCCTTGCTGGCGATCAGCATGGCGATCAGGTTGCC
GCCGGCGTAGCCGCCTCCGTTGCTGCCTGGCGCGTGCATTTCCTCGGCCCAGAGCTTGAGCATTTCATCG
ATGGCTTTGATCACGGTCGTGTCTCCTTTCACTGCCCGCGAGGGCTCGGCATGAGGTCGGTTCAACGGCG
CTCGTGGCGGTCCTGGCAGTCGATGCAGCGACTGCAACCCGGTGCCGCCCGGCGGCGCGCCTGGGGAATG
GGCTCGCCGCAGTCCTCGCAGTCTTCCGCGGACTCGCGGATGGCCAGCGCCGGGCGCGCCGCCAGGAGGC
CGTCGAGGCGAGCCAGGACCAGTTCGTTGGCGTGATCGGCAAGGTCAGCCATGGAAAGCCTCCCTGGCGT
GCACGACGCCCGTCGGCCGAATGGATGGAACATGACAGATGCGCGGCGCCGGTTCGGCGACCTGCGTTCC
TCTGCGCGGAAATGGCGAGTGCGTATCTCGCGACATGGCAATGACTCCCTGTACGACGCCGCCGTACCTG
AAGCAGGTCGGCGACAGAATGAATGAGCGGGACGGAGCGTCTGCATGGACGGCGCTCCGACCCTCCAGGT
GCCGGAGCGCATTTCCGTGGGCCCCGGCACCAAGCAGTCGGCACCTGCAT
Blast against PA14
Processed Seqquence ID: 12092
5' Trim Position: 1 3' Trim Position: 677
Processed Sequence:
(Transposon Sequence in Red)		 
GTCGTTTGTATACGACTCACTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTAGTGCTCCA
CCACCACGTTCTTCAGTTCCTCGGGTAGGCGATTTACCAGGCGATCGACCTCCGCCATACGGTCGAGGAT
CATCCTGTGGCCGCCTGGGCAGCGGACCACCTCGCCCTTGCTGGCGATCAGCATGGCGATCAGGTTGCCG
CCGGCGTAGCCGCCTCCGTTGCTGCCTGGCGCGTGCATTTCCTCGGCCCAGAGCTTGAGCATTTCATCGA
TGGCTTTGATCACGGTCGTGTCTCCTTTCACTGCCCGCGAGGGCTCGGCATGAGGTCGGTTCAACGGCGC
TCGTGGCGGTCCTGGCAGTCGATGCAGCGACTGCAACCCGGTGCCGCCCGGCGGCGCGCCTGGGGAATGG
GCTCGCCGCAGTCCTCGCAGTCTTCCGCGGACTCGCGGATGGCCAGCGCCGGGCGCGCCGCCAGGAGGCC
GTCGAGGCGAGCCAGGACCAGTTCGTTGGCGTGATCGGCAAGGTCAGCCATGGAAAGCCTCCCTGGCGTG
CACGACGCCCGTCGGCCGAATGGATGGAACATGACAGATGCGCGGCGCCGGTTCGGCGACCTGCGTTCCT
CTGCGCGGAAATGGCGAGTGCGTATCTCGCGACATGGCAATGACTC
Blast against PA14
BLAST of Processed Sequence against PA14 And PAO1 Genome Sequence
54609 54609 54610 54610
PA14 BLAST Hit ID: 54609       Show Transposon Map
Identities: 606/616 (98.37%) BLAST Bit Score: 999.9 Percent of Processed Sequence That Aligns: 90.98%
Query Start: 61 Query End: 676 Subject Start: 687589 Subject End: 686974 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 687589 PA14_07980 -
Raw Sequence Start: 687650 PA14_07980 -
Extrapolated Insert Location: 687588 PA14_07980 -
Default Offset Location: 687587 PA14_07980 -
PAO1 BLAST Hit ID: 54610       Show Transposon Map
Identities: 602/616 (97.72%) BLAST Bit Score: 999.9 Percent of Processed Sequence That Aligns: 90.98%
Query Start: 61 Query End: 676 Subject Start: 674899 Subject End: 674284 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 674899 PA0613 -
Raw Sequence Start: 674960 PA0613 -
Extrapolated Insert Location: 674898 PA0613 -
Default Offset Location: 674897 PA0613 -
H PA14 Blast Hit H PAO1 Blast Hit