Mutant Report for Mutant ID: 33139
Strain: PA14 Genetic Background: Wild Type Transposon: MrT7 Library: lib_04_04
Raw Sequence ID: 16915
Chromat Path: H:\Pa14\MrT7\346v1\346v1_B07_B7_061.ab1 Last Transposon Base: 59 Transposon Sequence
Match Quality: 0
Raw Sequence:
(Transposon Sequence in Red)		 
ACGTTTGTATACGACTCCTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTATTGACCAGCT
ATATCCGCGCGAAGATGAACCATTCGCGCACCCGCCCGCATGGCTCCAAGGTCTGGGCCAACGAAATCAT
GCATGGCGCGCCGCTGCTCGGCGCCACCCTCGACGACAGCCTCTACGAATGGGCCAAGATGAAGGAGGCG
AAGATCCGCCAGTGGGTCGAGGAAAAGCGCATCCTGTCGATCGAGCCGAGCAGCCTGCTCTACATGATCT
GGGCCTCCACCCAGCACTACGCCGACTTCGGCTACCAGGTCGAGGTGCTCAACGGCCACCAGCCGCTCAG
CGACATGCAGTTCGAAAGGGCGGTACAGACGGTGACCAGCGTGGTCCTGCGCGGAATCGGATTGGAGCCC
TGAGCAGCGGAGCCTTCGCTAGAAGGCTCCGGCGATGGCTCAGCTGGCGCGGGTGAAGTCCACCGCTTCC
GCCTGTTCGAGGAACACCGTGGTCTGCGCCGGGACCGTCTCGGCGAGCAGCCGTCCGCCGCGGATCGAGT
AGCGCACCGGGACCTGCCGGCGCACCGCGTCGAAGCCGTCGCGGGCCGGCAGCACCAGCAGGTTGGCCGG
ATGGCCGACCTCGATGCCGTATTCCCGCAGGCCGAGGGTGCGGGCGCTGTTGCGGGTGATCAGGTCGAGG
CCGCCGTCGATCTCGTCGTACCCACCGGGGGGGGGGGGTCGTGGCAAANNNNNNNNNNNNNNNNNNNNNN
TNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNTTNCNNNTNNNNGNTNTNN
NNTCTTCTTTTNTNNTTTCGTCGTGTTTTTTCTCTCGGTGCTTGTTTGTTTTTT
Blast against PA14
Processed Seqquence ID: 11981
5' Trim Position: 3 3' Trim Position: 722
Processed Sequence:
(Transposon Sequence in Red)		 
TTTGTATACGACTCCTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTATTGACCAGCTATA
TCCGCGCGAAGATGAACCATTCGCGCACCCGCCCGCATGGCTCCAAGGTCTGGGCCAACGAAATCATGCA
TGGCGCGCCGCTGCTCGGCGCCACCCTCGACGACAGCCTCTACGAATGGGCCAAGATGAAGGAGGCGAAG
ATCCGCCAGTGGGTCGAGGAAAAGCGCATCCTGTCGATCGAGCCGAGCAGCCTGCTCTACATGATCTGGG
CCTCCACCCAGCACTACGCCGACTTCGGCTACCAGGTCGAGGTGCTCAACGGCCACCAGCCGCTCAGCGA
CATGCAGTTCGAAAGGGCGGTACAGACGGTGACCAGCGTGGTCCTGCGCGGAATCGGATTGGAGCCCTGA
GCAGCGGAGCCTTCGCTAGAAGGCTCCGGCGATGGCTCAGCTGGCGCGGGTGAAGTCCACCGCTTCCGCC
TGTTCGAGGAACACCGTGGTCTGCGCCGGGACCGTCTCGGCGAGCAGCCGTCCGCCGCGGATCGAGTAGC
GCACCGGGACCTGCCGGCGCACCGCGTCGAAGCCGTCGCGGGCCGGCAGCACCAGCAGGTTGGCCGGATG
GCCGACCTCGATGCCGTATTCCCGCAGGCCGAGGGTGCGGGCGCTGTTGCGGGTGATCAGGTCGAGGCCG
CCGTCGATCTCGTCGTACC
Blast against PA14
BLAST of Processed Sequence against PA14 And PAO1 Genome Sequence
54109 54109 54110 54110 54111 54111 54112 54112 54113 54113 54114 54114 54115 54115 54116 54116 54117 54117 54118 54118 54119 54119 54120 54120 54121 54121
PA14 BLAST Hit ID: 54109       Show Transposon Map
Identities: 662/662 (100.00%) BLAST Bit Score: 999.9 Percent of Processed Sequence That Aligns: 91.93%
Query Start: 56 Query End: 717 Subject Start: 503737 Subject End: 504398 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 503737 PA14_05660 +
Raw Sequence Start: 503679 PA14_05660 +
Extrapolated Insert Location: 503738 PA14_05660 +
Default Offset Location: 503742 PA14_05660 +
PA14 BLAST Hit ID: 54110       Show Transposon Map
Identities: 43/49 (87.75%) BLAST Bit Score: 50.1 Percent of Processed Sequence That Aligns: 6.68%
Query Start: 103 Query End: 151 Subject Start: 4010614 Subject End: 4010662 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 4010614 PA14_44980 +
Raw Sequence Start: 4010509 PA14_44980 +
Extrapolated Insert Location: 4010568 PA14_44980 +
Default Offset Location: 4010572 PA14_44980 +
PA14 BLAST Hit ID: 54111       Show Transposon Map
Identities: 23/23 (100.00%) BLAST Bit Score: 46.1 Percent of Processed Sequence That Aligns: 3.06%
Query Start: 285 Query End: 307 Subject Start: 191078 Subject End: 191100 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 191078 PA14_02090 +
Raw Sequence Start: 190791 PA14_02090 +
Extrapolated Insert Location: 190850 PA14_02090 +
Default Offset Location: 190854 PA14_02090 +
PA14 BLAST Hit ID: 54112       Show Transposon Map
Identities: 21/21 (100.00%) BLAST Bit Score: 42.1 Percent of Processed Sequence That Aligns: 2.78%
Query Start: 606 Query End: 626 Subject Start: 74785 Subject End: 74805 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 74785 PA14_00760 -
Raw Sequence Start: 74177 PA14_00760 -
Extrapolated Insert Location: 74236 PA14_00760 -
Default Offset Location: 74240 PA14_00760 -
PA14 BLAST Hit ID: 54113       Show Transposon Map
Identities: 20/20 (100.00%) BLAST Bit Score: 40.1 Percent of Processed Sequence That Aligns: 2.64%
Query Start: 608 Query End: 627 Subject Start: 2189003 Subject End: 2188984 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 2189003 PA14_25010*,PA14_25020 +,-
Raw Sequence Start: 2189613 PA14_25010* +
Extrapolated Insert Location: 2189554 PA14_25010* +
Default Offset Location: 2189550 PA14_25010* +
PA14 BLAST Hit ID: 54114       Show Transposon Map
Identities: 23/24 (95.83%) BLAST Bit Score: 40.1 Percent of Processed Sequence That Aligns: 3.20%
Query Start: 529 Query End: 552 Subject Start: 1822008 Subject End: 1822031 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 1822008
Raw Sequence Start: 1821477 PA14_21060 -
Extrapolated Insert Location: 1821536 PA14_21060 -
Default Offset Location: 1821540 PA14_21060 -
PA14 BLAST Hit ID: 54115       Show Transposon Map
Identities: 20/20 (100.00%) BLAST Bit Score: 40.1 Percent of Processed Sequence That Aligns: 2.64%
Query Start: 571 Query End: 590 Subject Start: 1067361 Subject End: 1067380 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 1067361 PA14_12380*,PA14_12390 +,-
Raw Sequence Start: 1066788 PA14_12380*,PA14_12390 +,-
Extrapolated Insert Location: 1066847 PA14_12380*,PA14_12390 +,-
Default Offset Location: 1066851 PA14_12380*,PA14_12390 +,-
PAO1 BLAST Hit ID: 54116       Show Transposon Map
Identities: 657/662 (99.24%) BLAST Bit Score: 999.9 Percent of Processed Sequence That Aligns: 91.93%
Query Start: 56 Query End: 717 Subject Start: 488986 Subject End: 489647 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 488986 PA0436 +
Raw Sequence Start: 488928 PA0436 +
Extrapolated Insert Location: 488987 PA0436 +
Default Offset Location: 488991 PA0436 +
PAO1 BLAST Hit ID: 54117       Show Transposon Map
Identities: 43/49 (87.75%) BLAST Bit Score: 50.1 Percent of Processed Sequence That Aligns: 6.68%
Query Start: 103 Query End: 151 Subject Start: 1634201 Subject End: 1634153 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 1634201 PA1504 +
Raw Sequence Start: 1634306 PA1504 +
Extrapolated Insert Location: 1634247 PA1504 +
Default Offset Location: 1634243 PA1504 +
PAO1 BLAST Hit ID: 54118       Show Transposon Map
Identities: 23/23 (100.00%) BLAST Bit Score: 46.1 Percent of Processed Sequence That Aligns: 3.06%
Query Start: 285 Query End: 307 Subject Start: 192218 Subject End: 192240 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 192218 PA0167 +
Raw Sequence Start: 191931 PA0167 +
Extrapolated Insert Location: 191990 PA0167 +
Default Offset Location: 191994 PA0167 +
PAO1 BLAST Hit ID: 54119       Show Transposon Map
Identities: 21/21 (100.00%) BLAST Bit Score: 42.1 Percent of Processed Sequence That Aligns: 2.78%
Query Start: 606 Query End: 626 Subject Start: 77141 Subject End: 77161 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 77141 PA0064 -
Raw Sequence Start: 76533 PA0064 -
Extrapolated Insert Location: 76592 PA0064 -
Default Offset Location: 76596 PA0064 -
PAO1 BLAST Hit ID: 54120       Show Transposon Map
Identities: 23/24 (95.83%) BLAST Bit Score: 40.1 Percent of Processed Sequence That Aligns: 3.20%
Query Start: 529 Query End: 552 Subject Start: 3725225 Subject End: 3725202 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 3725225
Raw Sequence Start: 3725756 PA3323 -
Extrapolated Insert Location: 3725697 PA3323 -
Default Offset Location: 3725693 PA3323 -
PAO1 BLAST Hit ID: 54121       Show Transposon Map
Identities: 20/20 (100.00%) BLAST Bit Score: 40.1 Percent of Processed Sequence That Aligns: 2.64%
Query Start: 608 Query End: 627 Subject Start: 3380352 Subject End: 3380371 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 3380352 PA3019 -
Raw Sequence Start: 3379742
Extrapolated Insert Location: 3379801
Default Offset Location: 3379805
H PA14 Blast Hit H PAO1 Blast Hit