Mutant Report for Mutant ID: 31946
Strain: PA14 Genetic Background: Wild Type Transposon: MrT7 Library: lib_04_04
Raw Sequence ID: 14666
Chromat Path: H:\Pa14\MrT7\333v1\333v1_G02_G2_018.ab1 Last Transposon Base: 62 Transposon Sequence
Match Quality: 0
Raw Sequence:
(Transposon Sequence in Red)		 
TGTCAGTTTGTATACGACTCCTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTATACGCGC
CGCGGATCTTGTCTGAGGCATGGGCAGCAGGTCCTCGCGCTTGAGCAGCACCCGGTTGCCCAGGCGCTGG
GACAACTGCGGCGCGACCTGCAAGGGGGTTTCCACGGCGACGTCATACACCGGCGCGGCCAGGATGCTTT
GCACGTAGACAGCCAGCAGGGGTTTGCTTGCGCTGAGGGTCGAGGCGCTCATCGGCTGGCCTCCGCGCTC
GCCGCCACCGAGGCCAGCAGGTCGGTGCCGTAGGAGTTGTCCACCGCGCAGCGCCACCGGCCATCGGCCT
CCTGGCGGAACACGTAGGTGGCCCGACGTTGCAGGGGTTGGGCATTCGACTCCATCACCGCCTCGGCCAT
GACCAGCGCCATATCTCCTGCCTCTATCATCTGCGGGTCGTGCCCGCCCCCTTCCCGGGTTTGCTTCGTA
AATCCAATCACTTACTATCCCACAATAGACCAACCTGTCCCTTATCTCTCGTTTTTAACTTCCACTTCTC
TTTCACCATATCAACCTTAACACCATATATCCAGATTACCAAATAACCTACCATTAAATTATCAACCAAT
TTCACCACCCTTCTTCTATACTCATCTTCCGCCCCTTTTTAATCCCCTCTTCGACTAATCATTTTCCCCC
TACAAAATAAATTATTAATTTCCCCTTATTTTCCTTTTTTTACGTGGCCCACTTTCTATTCCTCTTATAT
TTCACTCTGTCGTTAGATCCACCTACTCTCAATTTATCTTTTTCACTTAAATATATATATTTTGAATACA
CAAATAACCTTATAAGCATAATAGAT
Blast against PA14
Processed Seqquence ID: 10232
5' Trim Position: 12 3' Trim Position: 366
Processed Sequence:
(Transposon Sequence in Red)		 
TACGACTCCTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTATACGCGCCGCGGATCTTGT
CTGAGGCATGGGCAGCAGGTCCTCGCGCTTGAGCAGCACCCGGTTGCCCAGGCGCTGGGACAACTGCGGC
GCGACCTGCAAGGGGGTTTCCACGGCGACGTCATACACCGGCGCGGCCAGGATGCTTTGCACGTAGACAG
CCAGCAGGGGTTTGCTTGCGCTGAGGGTCGAGGCGCTCATCGGCTGGCCTCCGCGCTCGCCGCCACCGAG
GCCAGCAGGTCGGTGCCGTAGGAGTTGTCCACCGCGCAGCGCCACCGGCCATCGGCCTCCTGGCGGAACA
CGTA
Blast against PA14
BLAST of Processed Sequence against PA14 And PAO1 Genome Sequence
46757 46757 46758 46758 46759 46759 46760 46760
PA14 BLAST Hit ID: 46757       Show Transposon Map
Identities: 266/270 (98.51%) BLAST Bit Score: 504.0 Percent of Processed Sequence That Aligns: 75.99%
Query Start: 85 Query End: 354 Subject Start: 4197401 Subject End: 4197670 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 4197401 PA14_47100 -
Raw Sequence Start: 4197305 PA14_47100 -
Extrapolated Insert Location: 4197367 PA14_47100 -
Default Offset Location: 4197368 PA14_47100 -
PA14 BLAST Hit ID: 46758       Show Transposon Map
Identities: 77/91 (84.61%) BLAST Bit Score: 69.9 Percent of Processed Sequence That Aligns: 25.42%
Query Start: 85 Query End: 175 Subject Start: 386392 Subject End: 386302 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 386392 PA14_04320 -
Raw Sequence Start: 386488 PA14_04320 -
Extrapolated Insert Location: 386426 PA14_04320 -
Default Offset Location: 386425 PA14_04320 -
PAO1 BLAST Hit ID: 46759       Show Transposon Map
Identities: 265/270 (98.14%) BLAST Bit Score: 496.0 Percent of Processed Sequence That Aligns: 75.99%
Query Start: 85 Query End: 354 Subject Start: 1437232 Subject End: 1436963 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 1437232 PA1326 -
Raw Sequence Start: 1437328 PA1326 -
Extrapolated Insert Location: 1437266 PA1326 -
Default Offset Location: 1437265 PA1326 -
PAO1 BLAST Hit ID: 46760       Show Transposon Map
Identities: 47/52 (90.38%) BLAST Bit Score: 63.9 Percent of Processed Sequence That Aligns: 14.41%
Query Start: 85 Query End: 136 Subject Start: 372223 Subject End: 372172 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 372223 PA0331 -
Raw Sequence Start: 372319 PA0331 -
Extrapolated Insert Location: 372257 PA0331 -
Default Offset Location: 372256 PA0331 -
H PA14 Blast Hit H PAO1 Blast Hit