Mutant Report for Mutant ID: 30874
Strain: PA14 Genetic Background: Wild Type Transposon: MrT7 Library: lib_04_04
Raw Sequence ID: 15322
Chromat Path: H:\Pa14\MrT7\322v1\322v1_E10_E10_083.ab1 Last Transposon Base: 64 Transposon Sequence
Match Quality: 0
Raw Sequence:
(Transposon Sequence in Red)		 
TGTTCAGTTTGTATACGACTCACTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTACGACC
CGCAGCGCCACTTCACCGACCACAGCGGCGTGCGCTTTCCCTTCGAGCAGTACGATCCGGAGGAAATGAT
CGCGCGGATGGTCGAGCTGGCCGAGGCCGACAAAGGACCGTTCCGCACCCTGTTGCCGGAAGCTTTCGTC
GAGGTGGTGAAGGACGATCATGTGCAGGCCTGGCAGCGCCGCCTGTAGCGGTCTGCCGCTGCGCCGGGGA
GGGCGGCGGCCGGATCGGCGGCCGCCGCTTCGCTCAGCCCCTGCGCAGCAGGACCCGGGTCACCCGGCGT
TCTTCCACTTCCACCACCCGCAGTTCCCAGTCCTGCCACTGCAGGCGGTCGCCGATCACCGGCAGGCGGT
CGAGCAGGCTCATCACCAGCCCGGCGAGGGTCTGGTAGTCCTCCGTGGCGCGCGCGTCGAACCCCACGCG
TCGACGCACCTGGGTGAGGTTCATGGCACCGCTGACCAGGTAGCCGTCGTTCTCCTCGACCACGTCCGGA
CCGTCTATCTCGCTGGCGTCAGGCAGCTCGCCGGCGATGGATTCGAGAATGTCGGTCATGTTCAGCCAGG
CCATGAAATTCCCGAAATCCTTGACCCCGAACGCCACCTGGGCCGAGGCCTTCCGGCATCTTCTCCAGGG
GCATTATAAAATTTAAAAAGTCCTTTATAAAGAGATAGCCGTATTTGCTGTGCTAGGTTTTTCCAGTGTC
CCGGCAGGGGCCCCCAGAAAAGTAACCTCCTTTGAAGACTTTTCCCTTTTTTGTTCAATCTAAACCACTA
ACGGACTTATCTCTNACTCTACCCCGTGACTCGATACTGACTGGTCTAGCGTAACTATGTATGAGAGCTA
TTGTCAAGCGCAGTGTCCTGTTCCGGGAATCGATCCTCN
Blast against PA14
Processed Seqquence ID: 10743
5' Trim Position: 4 3' Trim Position: 571
Processed Sequence:
(Transposon Sequence in Red)		 
CAGTTTGTATACGACTCACTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTACGACCCGCA
GCGCCACTTCACCGACCACAGCGGCGTGCGCTTTCCCTTCGAGCAGTACGATCCGGAGGAAATGATCGCG
CGGATGGTCGAGCTGGCCGAGGCCGACAAAGGACCGTTCCGCACCCTGTTGCCGGAAGCTTTCGTCGAGG
TGGTGAAGGACGATCATGTGCAGGCCTGGCAGCGCCGCCTGTAGCGGTCTGCCGCTGCGCCGGGGAGGGC
GGCGGCCGGATCGGCGGCCGCCGCTTCGCTCAGCCCCTGCGCAGCAGGACCCGGGTCACCCGGCGTTCTT
CCACTTCCACCACCCGCAGTTCCCAGTCCTGCCACTGCAGGCGGTCGCCGATCACCGGCAGGCGGTCGAG
CAGGCTCATCACCAGCCCGGCGAGGGTCTGGTAGTCCTCCGTGGCGCGCGCGTCGAACCCCACGCGTCGA
CGCACCTGGGTGAGGTTCATGGCACCGCTGACCAGGTAGCCGTCGTTCTCCTCGACCACGTCCGGACCGT
CTATCTC
Blast against PA14
BLAST of Processed Sequence against PA14 And PAO1 Genome Sequence
48883 48883 48884 48884 48885 48885 48886 48886
PA14 BLAST Hit ID: 48883       Show Transposon Map
Identities: 503/508 (99.01%) BLAST Bit Score: 967.0 Percent of Processed Sequence That Aligns: 89.42%
Query Start: 60 Query End: 567 Subject Start: 4191985 Subject End: 4191478 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 4191985 PA14_47050*,PA14_47060 -,+
Raw Sequence Start: 4192048 PA14_47050*,PA14_47060 -,+
Extrapolated Insert Location: 4191984 PA14_47050*,PA14_47060 -,+
Default Offset Location: 4191985 PA14_47050*,PA14_47060 -,+
PA14 BLAST Hit ID: 48884       Show Transposon Map
Identities: 20/20 (100.00%) BLAST Bit Score: 40.1 Percent of Processed Sequence That Aligns: 3.35%
Query Start: 284 Query End: 303 Subject Start: 4903722 Subject End: 4903703 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 4903722 PA14_55220,PA14_55210* -,-
Raw Sequence Start: 4904009 PA14_55220,PA14_55210* -,-
Extrapolated Insert Location: 4903945 PA14_55220,PA14_55210* -,-
Default Offset Location: 4903946 PA14_55220,PA14_55210* -,-
PAO1 BLAST Hit ID: 48885       Show Transposon Map
Identities: 497/508 (97.83%) BLAST Bit Score: 920.0 Percent of Processed Sequence That Aligns: 89.42%
Query Start: 60 Query End: 567 Subject Start: 1442654 Subject End: 1443161 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 1442654 PA1330 +
Raw Sequence Start: 1442591 PA1330 +
Extrapolated Insert Location: 1442655 PA1330 +
Default Offset Location: 1442654 PA1330 +
PAO1 BLAST Hit ID: 48886       Show Transposon Map
Identities: 20/20 (100.00%) BLAST Bit Score: 40.1 Percent of Processed Sequence That Aligns: 3.35%
Query Start: 284 Query End: 303 Subject Start: 776675 Subject End: 776694 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 776675 PA0703 -
Raw Sequence Start: 776388 PA0703 -
Extrapolated Insert Location: 776452 PA0703 -
Default Offset Location: 776451 PA0703 -
H PA14 Blast Hit H PAO1 Blast Hit