Mutant Report for Mutant ID: 30310
Strain: PA14 Genetic Background: Wild Type Transposon: MrT7 Library: lib_04_04
Raw Sequence ID: 14086
Chromat Path: H:\Pa14\MrT7\316v1\316v1_F10_F10_091.ab1 Last Transposon Base: 60 Transposon Sequence
Match Quality: 0
Raw Sequence:
(Transposon Sequence in Red)		 
TGTCGTTTGTATCGACTCCTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTATCCAGCGGC
ATCTCCGCGCCCTCCAGGCGAGGCGTCATGCGCGCCCCGCACACGGTCACCAGGGCGGCGCACTGGAACT
CCAGCTGCGGACCCAGCAGCGCGCACTCCAGCACGGCGGCGGTCGGTGCGTTGCCCACCAGCAGGTTGGC
CGCGCGCAGGGCGTACTGGTCGAGCGCGCCGGACGGCGGGATACCCAGGTGATAGTGGCCTTCGCGGCCG
GCGTCCTGCACCGAGGTGGCAAGGCCGGGCTTGAGCACCTTAATCGACATACAGCACCTCCTGCTCATCT
GGCGTGCTGGCTAGCGGATCGGCGAGGAACGCGTCAAGATCGAAACTCACCGGACGGATGCGCAGGTCGA
ATGTGCCCGCTTCGACTTCGGCCGCCATGCGCTCGTATTCGGTCCGCTCGATGGGGCGGAACTGGACGAT
GTCCCCAGCGCGGAAGAACACCATCGATTCCTCGAGATAAGGCAGGCCCTGGCGGGGATCGAAGATCGGC
GCCGGAGTCACGCCTAACATCTGGTAGACGCCGGTACCGTGCACCGAATAGATGCAACCGAAGCACCGCC
GTGGCCCAAGGTCCACTTCCGCGTGTCCATGCCCGGACACAGGTACTTGGGCCCTGAACCTGCCGATCGC
GCTCCACCATTAGGACATGAACGGACAGTCGGCCAAAAACACCTACCATCTAAACAAACACCGCCAGCCC
TTTGTCGCCGTGATAAACACCTCCCATCCGTCTTACCCCTTATTCCCCCCGCTATCTCTAGGTCCGGGAA
TACTGGGTCCTGTAACATTTATGCAAATCCCCTAAGTGTTCTAAGAGCCCAGGAATCTTTTACCAACCT
Blast against PA14
Processed Seqquence ID: 9748
5' Trim Position: 13 3' Trim Position: 485
Processed Sequence:
(Transposon Sequence in Red)		 
GACTCCTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTATCCAGCGGCATCTCCGCGCCCT
CCAGGCGAGGCGTCATGCGCGCCCCGCACACGGTCACCAGGGCGGCGCACTGGAACTCCAGCTGCGGACC
CAGCAGCGCGCACTCCAGCACGGCGGCGGTCGGTGCGTTGCCCACCAGCAGGTTGGCCGCGCGCAGGGCG
TACTGGTCGAGCGCGCCGGACGGCGGGATACCCAGGTGATAGTGGCCTTCGCGGCCGGCGTCCTGCACCG
AGGTGGCAAGGCCGGGCTTGAGCACCTTAATCGACATACAGCACCTCCTGCTCATCTGGCGTGCTGGCTA
GCGGATCGGCGAGGAACGCGTCAAGATCGAAACTCACCGGACGGATGCGCAGGTCGAATGTGCCCGCTTC
GACTTCGGCCGCCATGCGCTCGTATTCGGTCCGCTCGATGGGGCGGAACTGG
Blast against PA14
BLAST of Processed Sequence against PA14 And PAO1 Genome Sequence
44480 44480 44481 44481 44482 44482
PA14 BLAST Hit ID: 44480       Show Transposon Map
Identities: 414/426 (97.18%) BLAST Bit Score: 749.0 Percent of Processed Sequence That Aligns: 90.04%
Query Start: 47 Query End: 472 Subject Start: 568309 Subject End: 567884 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 568309 PA14_06470*,PA14_06480 +,-
Raw Sequence Start: 568368 PA14_06470*,PA14_06480 +,-
Extrapolated Insert Location: 568308 PA14_06470*,PA14_06480 +,-
Default Offset Location: 568305 PA14_06470*,PA14_06480 +,-
PAO1 BLAST Hit ID: 44481       Show Transposon Map
Identities: 403/426 (94.60%) BLAST Bit Score: 662.0 Percent of Processed Sequence That Aligns: 90.04%
Query Start: 47 Query End: 472 Subject Start: 555521 Subject End: 555096 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 555521 PA0496 -
Raw Sequence Start: 555580 PA0496 -
Extrapolated Insert Location: 555520 PA0496 -
Default Offset Location: 555517 PA0496 -
PAO1 BLAST Hit ID: 44482       Show Transposon Map
Identities: 20/20 (100.00%) BLAST Bit Score: 40.1 Percent of Processed Sequence That Aligns: 4.03%
Query Start: 162 Query End: 181 Subject Start: 4951701 Subject End: 4951682 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 4951701 PA4417 +
Raw Sequence Start: 4951875 PA4417 +
Extrapolated Insert Location: 4951815 PA4417 +
Default Offset Location: 4951812 PA4417 +
H PA14 Blast Hit H PAO1 Blast Hit