Mutant Report for Mutant ID: 27884
Strain: PA14 Genetic Background: Wild Type Transposon: MrT7 Library: lib_09_03
Raw Sequence ID: 11660
Chromat Path: H:\Pa14\MrT7\291v1\291v1_D08_D8_074.ab1 Last Transposon Base: 62 Transposon Sequence
Match Quality: 0
Raw Sequence:
(Transposon Sequence in Red)		 
TGTCGTTTGTATACGACTCACTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTACAGGGCA
GCTGGGCGCTGGAGCACCCGCAAGCGGTGCCCGGCGAGGCGACCTTCCATATCGTCATGGCCGGCACCTG
CTATTGCGAATTCCTCGATGGCGCTCGCCTCGACCTGCATCCCGGCGACCTGATCCTGTTGCCACGCGGC
ACCCCGCACCTCCTGCGCAGCGACAGCCCGGCGCAACCCTGCGAACCCACCGTGGAGCACCGCGGCAGCA
TCCCGCTGTACCAGTTGAACGGCCCCGGCGAGGCCCTCGACATGCTTTGCGGCAGCTACCGCTATCACGC
CGGTGCCAGCCTGTTCGGCGCGCTCCCCGAGAGCCTGCTGGTGCATATGGACGAGTCGACCCAGCAGCCC
CTGCGCGCGCTGATCGGGTTGATGCGCGAGGAAGCCGAGAGCACCCGCAGCGGCGCCCGCTCGATCATCG
ACGCGCTGGCCACCGCGCTGTTCGCCCTGGCCCTGCGCGCCTACCTGGACCGCCAACCGCTGGGCGACGG
CCTGTTCGGCCTGCTGAGTGACACCCGCCTGGAACGAGCCCTGCGGGCCATGGCTGGACGCCCGGAGCAT
GCCTGGACCCTGGAACAGCTTGCCCAGCAAGCGGGCAATTTCGCGGGCCATTTTCGTCCGCGCCTTCTCC
GACCTCGGCGGAAACCCTCCCTGAGTACTCCTAACCCCATACTCTGAAGGATTGGCAAAATTCTTATAAT
AAACATTAGAAAACCGTCCAATACTATATTATAAAAAACATAATCTAATAGATTATAGCATACATCACTA
ATGTTTATAGTGCGCACCTCGCCCATACATCTTTT
Blast against PA14
Processed Seqquence ID: 7605
5' Trim Position: 1 3' Trim Position: 565
Processed Sequence:
(Transposon Sequence in Red)		 
GTCGTTTGTATACGACTCACTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTACAGGGCAG
CTGGGCGCTGGAGCACCCGCAAGCGGTGCCCGGCGAGGCGACCTTCCATATCGTCATGGCCGGCACCTGC
TATTGCGAATTCCTCGATGGCGCTCGCCTCGACCTGCATCCCGGCGACCTGATCCTGTTGCCACGCGGCA
CCCCGCACCTCCTGCGCAGCGACAGCCCGGCGCAACCCTGCGAACCCACCGTGGAGCACCGCGGCAGCAT
CCCGCTGTACCAGTTGAACGGCCCCGGCGAGGCCCTCGACATGCTTTGCGGCAGCTACCGCTATCACGCC
GGTGCCAGCCTGTTCGGCGCGCTCCCCGAGAGCCTGCTGGTGCATATGGACGAGTCGACCCAGCAGCCCC
TGCGCGCGCTGATCGGGTTGATGCGCGAGGAAGCCGAGAGCACCCGCAGCGGCGCCCGCTCGATCATCGA
CGCGCTGGCCACCGCGCTGTTCGCCCTGGCCCTGCGCGCCTACCTGGACCGCCAACCGCTGGGCGACGGC
CTGT
Blast against PA14
BLAST of Processed Sequence against PA14 And PAO1 Genome Sequence
34265 34265 34266 34266 34267 34267 34268 34268 34269 34269 34270 34270
PA14 BLAST Hit ID: 34265       Show Transposon Map
Identities: 503/504 (99.80%) BLAST Bit Score: 991.0 Percent of Processed Sequence That Aligns: 89.18%
Query Start: 61 Query End: 564 Subject Start: 631485 Subject End: 630982 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 631485 PA14_07320*,PA14_07340 -,+
Raw Sequence Start: 631546 PA14_07340 +
Extrapolated Insert Location: 631484 PA14_07320*,PA14_07340 -,+
Default Offset Location: 631483 PA14_07320*,PA14_07340 -,+
PA14 BLAST Hit ID: 34266       Show Transposon Map
Identities: 25/25 (100.00%) BLAST Bit Score: 50.1 Percent of Processed Sequence That Aligns: 4.26%
Query Start: 498 Query End: 522 Subject Start: 5760798 Subject End: 5760822 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 5760798 PA14_64680 +
Raw Sequence Start: 5760300 PA14_64670 +
Extrapolated Insert Location: 5760362 PA14_64670 +
Default Offset Location: 5760363 PA14_64670 +
PA14 BLAST Hit ID: 34267       Show Transposon Map
Identities: 21/21 (100.00%) BLAST Bit Score: 42.1 Percent of Processed Sequence That Aligns: 3.55%
Query Start: 504 Query End: 524 Subject Start: 4595465 Subject End: 4595445 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 4595465 PA14_51720 +
Raw Sequence Start: 4595969 PA14_51730 +
Extrapolated Insert Location: 4595907 PA14_51730 +
Default Offset Location: 4595906 PA14_51730 +
PAO1 BLAST Hit ID: 34268       Show Transposon Map
Identities: 499/504 (99.00%) BLAST Bit Score: 959.0 Percent of Processed Sequence That Aligns: 89.18%
Query Start: 61 Query End: 564 Subject Start: 621510 Subject End: 621007 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 621510 PA0564 +
Raw Sequence Start: 621571 PA0564 +
Extrapolated Insert Location: 621509 PA0564 +
Default Offset Location: 621508 PA0564 +
PAO1 BLAST Hit ID: 34269       Show Transposon Map
Identities: 25/25 (100.00%) BLAST Bit Score: 50.1 Percent of Processed Sequence That Aligns: 4.26%
Query Start: 498 Query End: 522 Subject Start: 5489391 Subject End: 5489415 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 5489391 PA4894 +
Raw Sequence Start: 5488893 PA4893 +
Extrapolated Insert Location: 5488955 PA4893 +
Default Offset Location: 5488956 PA4893 +
PAO1 BLAST Hit ID: 34270       Show Transposon Map
Identities: 21/21 (100.00%) BLAST Bit Score: 42.1 Percent of Processed Sequence That Aligns: 3.55%
Query Start: 504 Query End: 524 Subject Start: 1056070 Subject End: 1056090 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 1056070 PA0972 +
Raw Sequence Start: 1055566 PA0971 +
Extrapolated Insert Location: 1055628 PA0971 +
Default Offset Location: 1055629 PA0971 +
H PA14 Blast Hit H PAO1 Blast Hit