Mutant Report for Mutant ID: 24023
Strain: PA14 Genetic Background: Wild Type Transposon: MrT7 Library: lib_09_03
Raw Sequence ID: 7799
Chromat Path: H:\Pa14\MrT7\251v1\251v1_B11_a_093.ab1 Last Transposon Base: 63 Transposon Sequence
Match Quality: 0
Raw Sequence:
(Transposon Sequence in Red)		 
TGTTCAGTTTGTATACGACTCCTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTACAACTA
TTCGATGGCCGGGGTGCTGAAGAATGCCATCGACTGGGCCTCGCGGCCGCCGGAGCAGCCGTTCTTCGGC
AAGCCGGCGGCGATCCTCGGCGCCAGCGCCGGGCGTTTCGGCACCGCGCGGGCGCAGTATCACCTGCGCC
AGACGCTGGTGTTCCTCGACGTTCATCCGCTGAACAAGCCGGAAGTGATGATCTCCAGCGCGCAGAACGC
CTTCGATGCCCAGGGCCGGCTGCTCGACGACAAGGCGCGCGAGCTGATCCAGCAGCAGTTGCAGGCCCTG
CAACTATGGGTGCGCCGCCTGCGCGGTTGAATGCCTCGTCACCGGAGAGCCTGAACATGAGCAATACCGA
AGAGCGTTGCGACCTGTCCTCCTCGCGGGACTGTCCGGAGCGCCGCGAACACCTGCTGCAACGAGTCACC
GCGCGCGCCGCGGAAATCGGCGGCGGGATCACCGTCAGTCGGCTGATGCCGTCGCGCCAGCGGCGGATGA
TCGGCGCCTGGTGCTTCCTCGACCATGCCGGGCCCGCCCAAATCTGCCCGGGCGGCCACTGGGCGGGGGG
CCTCACCACGACATGGGATTGGTGACCGAACAATATATGACTAAGAGATTAGTGTTTATAACAAGTAATA
TATTATTATATAATATATCTTCACTATTATATATTTGCAACCTAAACTTTAATATGTGTATCATTACATT
TATATAAGTGAACATATTGCGGACATCCACTTGCCTATATGTACTCATTTCATCTTGTATCAGATATAAA
TGAAACGTGAGCGTATAACATTTAAATGCGATTAACCATATCAAAACCATCCTGTGAACAGATATAATCG
GGACAGACTAACG
Blast against PA14
Processed Seqquence ID: 4116
5' Trim Position: 3 3' Trim Position: 588
Processed Sequence:
(Transposon Sequence in Red)		 
TCAGTTTGTATACGACTCCTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTACAACTATTC
GATGGCCGGGGTGCTGAAGAATGCCATCGACTGGGCCTCGCGGCCGCCGGAGCAGCCGTTCTTCGGCAAG
CCGGCGGCGATCCTCGGCGCCAGCGCCGGGCGTTTCGGCACCGCGCGGGCGCAGTATCACCTGCGCCAGA
CGCTGGTGTTCCTCGACGTTCATCCGCTGAACAAGCCGGAAGTGATGATCTCCAGCGCGCAGAACGCCTT
CGATGCCCAGGGCCGGCTGCTCGACGACAAGGCGCGCGAGCTGATCCAGCAGCAGTTGCAGGCCCTGCAA
CTATGGGTGCGCCGCCTGCGCGGTTGAATGCCTCGTCACCGGAGAGCCTGAACATGAGCAATACCGAAGA
GCGTTGCGACCTGTCCTCCTCGCGGGACTGTCCGGAGCGCCGCGAACACCTGCTGCAACGAGTCACCGCG
CGCGCCGCGGAAATCGGCGGCGGGATCACCGTCAGTCGGCTGATGCCGTCGCGCCAGCGGCGGATGATCG
GCGCCTGGTGCTTCCTCGACCATGC
Blast against PA14
BLAST of Processed Sequence against PA14 And PAO1 Genome Sequence
17618 17618 17619 17619
PA14 BLAST Hit ID: 17618       Show Transposon Map
Identities: 525/526 (99.80%) BLAST Bit Score: 999.9 Percent of Processed Sequence That Aligns: 89.74%
Query Start: 60 Query End: 585 Subject Start: 4334216 Subject End: 4333691 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 4334216 PA14_48740 +
Raw Sequence Start: 4334278 PA14_48740 +
Extrapolated Insert Location: 4334215 PA14_48740 +
Default Offset Location: 4334215 PA14_48740 +
PAO1 BLAST Hit ID: 17619       Show Transposon Map
Identities: 518/526 (98.47%) BLAST Bit Score: 979.0 Percent of Processed Sequence That Aligns: 89.74%
Query Start: 60 Query End: 585 Subject Start: 1304132 Subject End: 1304657 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 1304132 PA1204 +
Raw Sequence Start: 1304070 PA1204 +
Extrapolated Insert Location: 1304133 PA1204 +
Default Offset Location: 1304133 PA1204 +
H PA14 Blast Hit H PAO1 Blast Hit