Mutant Report for Mutant ID: 23548
Strain: PA14 Genetic Background: Wild Type Transposon: MrT7 Library: lib_09_03
Raw Sequence ID: 7324
Chromat Path: H:\Pa14\MrT7\246v1\246v1_C04_a_024.ab1 Last Transposon Base: 63 Transposon Sequence
Match Quality: 0
Raw Sequence:
(Transposon Sequence in Red)		 
TGTTCAGTTTGTATACGACTCCTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTATGCCCT
GCACCTGCTCACTCTGGACGGGTAGGAAAACCACGGCTACGCCGAGCAGCAGTACCGCAGCCTCACCGCC
GACGCCAACCCGAAGATCGCCGACCTCTACATCCCGGTGGCCGGCGGCGAGGTGAACGCCTTCGGCAACA
CGGTGAAGGAGATCGGCACGGTGTTCGCCGACCTGGTCCACGACGCCGGCAACCGCAAGCCGCAGGCGCC
GCGCTTCGACGCCGCGCCAAGCGTGGCGAGCAAGTCGGCGGCCATCGGCTACGCCATGCAGATGGAATTC
CTCGGTCGCCGCGACCCGGTGCGCGCGCCGCAGGTCGTCACCGCCTGGACCGCCGACCGCGACCTGACCA
ACCCGGCGCTGCCGGCGTTCCAGGTCTGCGTGCTGCTGAGCAAGCTGCAACTCAACGAACTGCAACAGTC
GCTGAAACTGATCGTCGACGCGGCCAAACGCACCCAGACCTCGCCCAAAGACTTCTTCCAGGAAATCGCC
AGCGGCAGCGTCTACATGAGCCGCGAACCCGGCGCAACTGGTGAAGGGCAACAACCTGGGGTCATAAGGG
CTTGGCTCGGCGAAAACCTGGGAAGGCCTGCCGTAACGCCACTAGGCTCTTGAACTTAACCCAGAACTTC
CGGCTTGTTCTTAAACGTTCCCCAAACACAGGGCCTCTTTTAAGAAATGGAGTCAAAAAACCCCCTTTTT
AAAACTTTCCTAGAGGAATTTCGCCAAATGGGGCCCGGGCGGGGCTACCCTCAATCCCGGAGATAGCGCT
TTACCCCGGGGGCCCATGGGAACCCGTCCAAAAAACTGTGCCCGGAA
Blast against PA14
Processed Seqquence ID: 3692
5' Trim Position: 7 3' Trim Position: 466
Processed Sequence:
(Transposon Sequence in Red)		 
TTTGTATACGACTCCTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTATGCCCTGCACCTG
CTCACTCTGGACGGGTAGGAAAACCACGGCTACGCCGAGCAGCAGTACCGCAGCCTCACCGCCGACGCCA
ACCCGAAGATCGCCGACCTCTACATCCCGGTGGCCGGCGGCGAGGTGAACGCCTTCGGCAACACGGTGAA
GGAGATCGGCACGGTGTTCGCCGACCTGGTCCACGACGCCGGCAACCGCAAGCCGCAGGCGCCGCGCTTC
GACGCCGCGCCAAGCGTGGCGAGCAAGTCGGCGGCCATCGGCTACGCCATGCAGATGGAATTCCTCGGTC
GCCGCGACCCGGTGCGCGCGCCGCAGGTCGTCACCGCCTGGACCGCCGACCGCGACCTGACCAACCCGGC
GCTGCCGGCGTTCCAGGTCTGCGTGCTGCTGAGCAAGCT
Blast against PA14
BLAST of Processed Sequence against PA14 And PAO1 Genome Sequence
15379 15379 15380 15380 15381 15381 15382 15382 15383 15383 15384 15384 15385 15385 15386 15386
PA14 BLAST Hit ID: 15379       Show Transposon Map
Identities: 397/404 (98.26%) BLAST Bit Score: 745.0 Percent of Processed Sequence That Aligns: 87.80%
Query Start: 56 Query End: 459 Subject Start: 85442 Subject End: 85039 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 85442 PA14_00870* +
Raw Sequence Start: 85504 PA14_00870* +
Extrapolated Insert Location: 85441 PA14_00870* +
Default Offset Location: 85441 PA14_00870* +
PA14 BLAST Hit ID: 15380       Show Transposon Map
Identities: 24/25 (96.00%) BLAST Bit Score: 42.1 Percent of Processed Sequence That Aligns: 5.23%
Query Start: 423 Query End: 447 Subject Start: 5404352 Subject End: 5404376 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 5404352 PA14_60630 +
Raw Sequence Start: 5403923 PA14_60630 +
Extrapolated Insert Location: 5403986 PA14_60630 +
Default Offset Location: 5403986 PA14_60630 +
PA14 BLAST Hit ID: 15381       Show Transposon Map
Identities: 20/20 (100.00%) BLAST Bit Score: 40.1 Percent of Processed Sequence That Aligns: 4.14%
Query Start: 418 Query End: 437 Subject Start: 6193361 Subject End: 6193342 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 6193361 PA14_69440 +
Raw Sequence Start: 6193785 PA14_69450,PA14_69460* +,-
Extrapolated Insert Location: 6193722 PA14_69450,PA14_69460* +,-
Default Offset Location: 6193722 PA14_69450,PA14_69460* +,-
PA14 BLAST Hit ID: 15382       Show Transposon Map
Identities: 20/20 (100.00%) BLAST Bit Score: 40.1 Percent of Processed Sequence That Aligns: 4.14%
Query Start: 236 Query End: 255 Subject Start: 1345033 Subject End: 1345014 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 1345033 PA14_15780 +
Raw Sequence Start: 1345275 PA14_15790 +
Extrapolated Insert Location: 1345212 PA14_15790 +
Default Offset Location: 1345212 PA14_15790 +
PAO1 BLAST Hit ID: 15383       Show Transposon Map
Identities: 396/404 (98.01%) BLAST Bit Score: 737.0 Percent of Processed Sequence That Aligns: 87.80%
Query Start: 56 Query End: 459 Subject Start: 87797 Subject End: 87394 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 87797 PA0074 +
Raw Sequence Start: 87859 PA0074 +
Extrapolated Insert Location: 87796 PA0074 +
Default Offset Location: 87796 PA0074 +
PAO1 BLAST Hit ID: 15384       Show Transposon Map
Identities: 24/25 (96.00%) BLAST Bit Score: 42.1 Percent of Processed Sequence That Aligns: 5.23%
Query Start: 423 Query End: 447 Subject Start: 5131884 Subject End: 5131908 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 5131884 PA4582 +
Raw Sequence Start: 5131455 PA4582 +
Extrapolated Insert Location: 5131518 PA4582 +
Default Offset Location: 5131518 PA4582 +
PAO1 BLAST Hit ID: 15385       Show Transposon Map
Identities: 20/20 (100.00%) BLAST Bit Score: 40.1 Percent of Processed Sequence That Aligns: 4.14%
Query Start: 418 Query End: 437 Subject Start: 5921146 Subject End: 5921127 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 5921146 PA5259 +
Raw Sequence Start: 5921570 PA5260 +
Extrapolated Insert Location: 5921507 PA5260 +
Default Offset Location: 5921507 PA5260 +
PAO1 BLAST Hit ID: 15386       Show Transposon Map
Identities: 20/20 (100.00%) BLAST Bit Score: 40.1 Percent of Processed Sequence That Aligns: 4.14%
Query Start: 236 Query End: 255 Subject Start: 4212970 Subject End: 4212989 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 4212970 PA3761 +
Raw Sequence Start: 4212728 PA3760 +
Extrapolated Insert Location: 4212791 PA3760 +
Default Offset Location: 4212791 PA3760 +
H PA14 Blast Hit H PAO1 Blast Hit